FastQCFastQC Report
Mon 16 Jan 2017
ERR1146560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028412
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT615231.5272271058670266No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG441251.0953447661262055No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA191130.47445494651490466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA164480.4082998461924947No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA151410.37585529980548166No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT143660.3566169498055313No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC126060.31292727754757954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113870.2826672147734641No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA108330.26891489748317704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106430.26419839877351176No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA103610.2571981217412717No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG96030.23838177425744936No Hit
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG65460.16249579238667744No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG57780.1434312081286621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.1430092056125342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT120600.021.90061812-13
TACCTGG41650.021.58032
GTACCCG5050.021.4339681
CTAACGC4950.020.8936463
CATGGGG70650.020.0951614
AGTACTT128750.019.85724412-13
GTACTTT134950.019.39767514-15
TAACGCC5750.018.804284
ACCTGGG46900.018.6439043
GTACCTG50550.018.2473981
TGCGCGA9450.017.4070594
ACTTTTT153350.017.13160516-17
ATGGGGG43350.017.025795
TAGTACT20000.016.6887994
GTCCTAC14250.016.512821
TACTTTT155550.016.4511114-15
GAGTACG13500.016.3843861
TCTAACG5800.016.2271082
GGGCTAT18700.015.8337016
CATGGGT20250.015.7863094
Produced by FastQC (version 0.11.5)