##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4028412
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.442103488918214	33.0	31.0	34.0	31.0	34.0
2	32.49655199120646	34.0	31.0	34.0	31.0	34.0
3	32.69386646648853	34.0	31.0	34.0	31.0	34.0
4	36.14163149151577	37.0	35.0	37.0	35.0	37.0
5	36.16132858307442	37.0	35.0	37.0	35.0	37.0
6	36.277216928159284	37.0	37.0	37.0	35.0	37.0
7	36.28725463036055	37.0	37.0	37.0	35.0	37.0
8	36.29723325220956	37.0	37.0	37.0	35.0	37.0
9	38.114819189298416	39.0	39.0	39.0	37.0	39.0
10-11	38.089317204893646	39.0	38.0	39.0	37.0	39.0
12-13	38.07352103012303	39.0	38.0	39.0	37.0	39.0
14-15	39.474596813831354	41.0	39.0	41.0	37.0	41.0
16-17	39.45931709566946	41.0	39.0	41.0	36.5	41.0
18-19	39.56404074359822	41.0	39.5	41.0	37.0	41.0
20-21	39.61114416797488	41.0	40.0	41.0	37.0	41.0
22-23	39.57247520859336	41.0	39.5	41.0	37.0	41.0
24-25	39.549643383050196	41.0	39.0	41.0	37.0	41.0
26-27	39.43586976704468	41.0	39.0	41.0	36.0	41.0
28-29	39.32710904445722	41.0	39.0	41.0	36.0	41.0
30-31	39.28095636692572	41.0	39.0	41.0	36.0	41.0
32-33	39.17952731746405	41.0	39.0	41.0	35.0	41.0
34-35	39.09341174636557	41.0	39.0	41.0	35.0	41.0
36-37	39.005895251031916	40.5	39.0	41.0	35.0	41.0
38-39	38.910428848886355	40.0	39.0	41.0	35.0	41.0
40-41	38.84478362689814	40.0	38.0	41.0	35.0	41.0
42-43	38.767485426018986	40.0	38.0	41.0	35.0	41.0
44-45	38.66656898549602	40.0	38.0	41.0	35.0	41.0
46-47	38.48124943526133	40.0	38.0	41.0	35.0	41.0
48-49	38.29206756905699	40.0	38.0	41.0	34.0	41.0
50-51	38.12802848864516	40.0	38.0	41.0	34.0	41.0
52-53	38.03172912800379	40.0	38.0	41.0	34.0	41.0
54-55	37.915959067741824	40.0	37.0	41.0	34.0	41.0
56-57	37.68383497020662	40.0	37.0	41.0	33.0	41.0
58-59	37.53035898512863	40.0	36.5	41.0	33.0	41.0
60-61	37.307928161270496	39.0	36.0	41.0	33.0	41.0
62-63	37.0575144746863	39.0	35.5	41.0	32.0	41.0
64-65	36.772974313451556	39.0	35.0	41.0	32.0	41.0
66-67	36.4520649824298	38.0	35.0	40.0	31.5	41.0
68-69	36.09539850938782	37.0	35.0	40.0	31.0	41.0
70-71	35.689997448125965	37.0	35.0	39.0	31.0	41.0
72-73	35.327074539545606	36.0	35.0	39.0	31.0	41.0
74-75	35.00090879483032	36.0	35.0	38.5	31.0	40.0
76-77	34.64508632682059	35.0	34.5	37.0	30.5	39.5
78-79	34.31707047342725	35.0	34.0	37.0	30.0	39.0
80-81	34.028747183753794	35.0	34.0	36.0	30.0	39.0
82-83	33.75547200733192	35.0	34.0	36.0	29.5	37.0
84-85	33.56991501862272	35.0	34.0	36.0	29.0	37.0
86-87	33.431040941194695	35.0	34.0	35.0	29.0	37.0
88-89	33.24923133482871	35.0	34.0	35.0	29.0	36.0
90-91	33.12661043607258	35.0	34.0	35.0	29.0	36.0
92-93	33.03406789077185	35.0	34.0	35.0	29.0	36.0
94-95	32.917805576986666	35.0	34.0	35.0	29.0	36.0
96-97	32.85903291421036	35.0	34.0	35.0	29.0	35.0
98-99	32.8477562622691	35.0	34.0	35.0	29.0	35.0
100	32.206373628119465	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23249001163565453
1101	2	0.254647204986874
1101	3	0.28380434254304987
1101	4	0.18313766152768096
1101	5	0.1892188200468894
1101	6	0.10756695561303786
1101	7	0.11898098130377832
1101	8	0.15511201577066913
1101	9	0.22739792246038348
1101	10-11	0.22584455377036505
1101	12-13	0.2133866120419512
1101	14-15	0.2903345285618997
1101	16-17	0.302957884858877
1101	18-19	0.3114381562832733
1101	20-21	0.33299926937736046
1101	22-23	0.3185784810444119
1101	24-25	0.28980395011215165
1101	26-27	0.2790529400616464
1101	28-29	0.3437913897949798
1101	30-31	0.3556526643462803
1101	32-33	0.3276565587049518
1101	34-35	0.3365568941719559
1101	36-37	0.4338363300935413
1101	38-39	0.4318442157541824
1101	40-41	0.42444922190714607
1101	42-43	0.4093393792419775
1101	44-45	0.41495056010037956
1101	46-47	0.4243658461413844
1101	48-49	0.39355386969737793
1101	50-51	0.3782904497390618
1101	52-53	0.4485018913712153
1101	54-55	0.4056803586220852
1101	56-57	0.4050351792076867
1101	58-59	0.3604237141276343
1101	60-61	0.3806443284488026
1101	62-63	0.3736534918981036
1101	64-65	0.4697448332996714
1101	66-67	0.38047052658798464
1101	68-69	0.43658528308392164
1101	70-71	0.39370114405519274
1101	72-73	0.32102729733988156
1101	74-75	0.3373857362354826
1101	76-77	0.3544838876954657
1101	78-79	0.3216017201378989
1101	80-81	0.2729092582193715
1101	82-83	0.2890334027994683
1101	84-85	0.29948577272139687
1101	86-87	0.2733893985282947
1101	88-89	0.3195963503028949
1101	90-91	0.3003017232744156
1101	92-93	0.28030184193031715
1101	94-95	0.24235599755628812
1101	96-97	0.25684323782785157
1101	98-99	0.23739196663078133
1101	100	0.29450687586531643
1102	1	0.2102443436026462
1102	2	0.23873311264333097
1102	3	0.25368035160563096
1102	4	0.17306164898053567
1102	5	0.09522165491416956
1102	6	0.13396099100279457
1102	7	0.13918704732844134
1102	8	0.16368004877659104
1102	9	0.1965486969578052
1102	10-11	0.22757241163992603
1102	12-13	0.23080913646766277
1102	14-15	0.3208575474715474
1102	16-17	0.2890917288192938
1102	18-19	0.3216618480232398
1102	20-21	0.3211964737453741
1102	22-23	0.3137992904757212
1102	24-25	0.3495601708341667
1102	26-27	0.3431756717284742
1102	28-29	0.35907275448347065
1102	30-31	0.32477049763457444
1102	32-33	0.28703595613009725
1102	34-35	0.31579867163510045
1102	36-37	0.33027048851710106
1102	38-39	0.3841497572160222
1102	40-41	0.36754480104226417
1102	42-43	0.33569371547793025
1102	44-45	0.3193860382841791
1102	46-47	0.3342809539867915
1102	48-49	0.29133865540679693
1102	50-51	0.2868826484310887
1102	52-53	0.27864025425459005
1102	54-55	0.2647864326340894
1102	56-57	0.31475124446378544
1102	58-59	0.3632457028504703
1102	60-61	0.3597605635133476
1102	62-63	0.4133542295926276
1102	64-65	0.3700015673162369
1102	66-67	0.33955326457228097
1102	68-69	0.3195151296597203
1102	70-71	0.34326555077515053
1102	72-73	0.28817269323740646
1102	74-75	0.23846579306557913
1102	76-77	0.2667536980332912
1102	78-79	0.2202750888352938
1102	80-81	0.26216780307850485
1102	82-83	0.2817713463734961
1102	84-85	0.3289284444205194
1102	86-87	0.23918748577351323
1102	88-89	0.21035509854146284
1102	90-91	0.21280212207611982
1102	92-93	0.22702696909243514
1102	94-95	0.25594723497592753
1102	96-97	0.22742645524156302
1102	98-99	0.23236561328636185
1102	100	0.14305292594093544
1103	1	0.1793509718830819
1103	2	0.21470342260691666
1103	3	0.24599314645347903
1103	4	0.14957377600150323
1103	5	0.1635473669883254
1103	6	0.11228094987617254
1103	7	0.07489382682361878
1103	8	0.089766173430462
1103	9	0.13166166684968061
1103	10-11	0.1501654766330489
1103	12-13	0.17862858906084256
1103	14-15	0.2572992856082408
1103	16-17	0.25736706802997844
1103	18-19	0.19288030553974522
1103	20-21	0.2074684373603617
1103	22-23	0.2213181411017331
1103	24-25	0.2552722435107242
1103	26-27	0.24977326325328875
1103	28-29	0.25438968307152265
1103	30-31	0.24922042870193195
1103	32-33	0.27067006350646494
1103	34-35	0.23870065962149312
1103	36-37	0.24631609818295885
1103	38-39	0.21438939847780603
1103	40-41	0.24197100635040414
1103	42-43	0.23516359489443062
1103	44-45	0.23462404396252623
1103	46-47	0.2556524899834187
1103	48-49	0.21417929514733913
1103	50-51	0.29071900311313925
1103	52-53	0.2241135894373656
1103	54-55	0.22598873326356994
1103	56-57	0.3068750208917024
1103	58-59	0.2472825971456274
1103	60-61	0.19386948930223014
1103	62-63	0.1669632925368134
1103	64-65	0.19588415187678976
1103	66-67	0.19274855781949185
1103	68-69	0.18865495773228247
1103	70-71	0.195118356549564
1103	72-73	0.19135342043636427
1103	74-75	0.23901312000158725
1103	76-77	0.23827535418595147
1103	78-79	0.22268897035692703
1103	80-81	0.22990633589323295
1103	82-83	0.19085559419260534
1103	84-85	0.14162605584746046
1103	86-87	0.11693581507847028
1103	88-89	0.12916709253399716
1103	90-91	0.15764389204438345
1103	92-93	0.09116336513925916
1103	94-95	0.14378052808838504
1103	96-97	0.17917445129130982
1103	98-99	0.16603493951104298
1103	100	0.2044549763109913
1104	1	0.1618588425108456
1104	2	0.19300062106479032
1104	3	0.2456722009043446
1104	4	0.17954085520492669
1104	5	0.14142259578932226
1104	6	0.11064265277111929
1104	7	0.10785237136855841
1104	8	0.1258244058751714
1104	9	0.19529474026744964
1104	10-11	0.18972703692142545
1104	12-13	0.14765883593153006
1104	14-15	0.24008557825558796
1104	16-17	0.2830414127278189
1104	18-19	0.2496478304571923
1104	20-21	0.2363173689575575
1104	22-23	0.2736352327760372
1104	24-25	0.2700080686292452
1104	26-27	0.2568906170635614
1104	28-29	0.33259821417642854
1104	30-31	0.3322023401194798
1104	32-33	0.28185064415173855
1104	34-35	0.3116396791595264
1104	36-37	0.2686041161935506
1104	38-39	0.2493959724188315
1104	40-41	0.2807682667676161
1104	42-43	0.288974979549387
1104	44-45	0.2792966270064028
1104	46-47	0.25371759916056646
1104	48-49	0.2356664767611889
1104	50-51	0.21498682676627112
1104	52-53	0.26579210867466685
1104	54-55	0.2393435093178482
1104	56-57	0.2774607300151004
1104	58-59	0.3151156679579046
1104	60-61	0.21029835486400827
1104	62-63	0.2774696031023396
1104	64-65	0.24356624906001656
1104	66-67	0.2769306440645778
1104	68-69	0.24117532662583585
1104	70-71	0.17935571636017045
1104	72-73	0.13357983305414223
1104	74-75	0.14861582136585838
1104	76-77	0.14767762259678818
1104	78-79	0.1401097507946787
1104	80-81	0.17257432955621255
1104	82-83	0.11506251193162598
1104	84-85	0.20452476441383993
1104	86-87	0.18591322883392536
1104	88-89	0.23368501212843285
1104	90-91	0.19278448006507887
1104	92-93	0.221897848326158
1104	94-95	0.22434981737061577
1104	96-97	0.16742707167652782
1104	98-99	0.20320533255802786
1104	100	0.24697460815343675
1105	1	0.13661762714421855
1105	2	0.1794185057035378
1105	3	0.18661976437032735
1105	4	0.144818615279398
1105	5	0.11027036944481239
1105	6	0.09898722769695922
1105	7	0.08915567877023278
1105	8	0.07524016961509972
1105	9	0.08791659411662778
1105	10-11	0.1048217903876747
1105	12-13	0.09948184515260294
1105	14-15	0.13212359810068364
1105	16-17	0.15666324316999436
1105	18-19	0.14451104471690002
1105	20-21	0.14806033685252373
1105	22-23	0.194492063183624
1105	24-25	0.18807342459914622
1105	26-27	0.19383325042722532
1105	28-29	0.2231611629836152
1105	30-31	0.19002092346528343
1105	32-33	0.1812460252664394
1105	34-35	0.19079684990878576
1105	36-37	0.2508675573123895
1105	38-39	0.23914801289127752
1105	40-41	0.19631818198430295
1105	42-43	0.19209281767542308
1105	44-45	0.25677888329630605
1105	46-47	0.23903250782345964
1105	48-49	0.3063341127709762
1105	50-51	0.28790236191554897
1105	52-53	0.2982220688725121
1105	54-55	0.2923270121543524
1105	56-57	0.29311926427971
1105	58-59	0.274787188038907
1105	60-61	0.2758632330100781
1105	62-63	0.25634300294706946
1105	64-65	0.2788580371083924
1105	66-67	0.25320830094300817
1105	68-69	0.2686861761980879
1105	70-71	0.2565413891475501
1105	72-73	0.16406126857630454
1105	74-75	0.16487899769101233
1105	76-77	0.21419879399137187
1105	78-79	0.24807027422009753
1105	80-81	0.20976133922395945
1105	82-83	0.21490567513760794
1105	84-85	0.1515985540860072
1105	86-87	0.21652580311760516
1105	88-89	0.20964685667507155
1105	90-91	0.22244302896320534
1105	92-93	0.22651498555010363
1105	94-95	0.24614864852398455
1105	96-97	0.20109743703454797
1105	98-99	0.14679452596922005
1105	100	0.27171732406731763
1106	1	0.1324279422028809
1106	2	0.15608784044680135
1106	3	0.1501857662898871
1106	4	0.10313246660457764
1106	5	0.10455857126058987
1106	6	0.06781036609029911
1106	7	0.08533624270790341
1106	8	0.0819337758085723
1106	9	0.08328092541947996
1106	10-11	0.10106019683750844
1106	12-13	0.12351261623305021
1106	14-15	0.1795066977682538
1106	16-17	0.1688490164049412
1106	18-19	0.19013982485365943
1106	20-21	0.14625120420462423
1106	22-23	0.15435450340238077
1106	24-25	0.1373579587957252
1106	26-27	0.15330704450315125
1106	28-29	0.22910725016487277
1106	30-31	0.22118153362963255
1106	32-33	0.18421712026179904
1106	34-35	0.21279486533944691
1106	36-37	0.2054085810806825
1106	38-39	0.22059894797244795
1106	40-41	0.23746342585771174
1106	42-43	0.17668660814131698
1106	44-45	0.18760592866259884
1106	46-47	0.1662234270854981
1106	48-49	0.21937879839944685
1106	50-51	0.28146612807844207
1106	52-53	0.25938672296371834
1106	54-55	0.245488146467153
1106	56-57	0.2875509521308359
1106	58-59	0.26951812332203673
1106	60-61	0.24588771588681624
1106	62-63	0.24751823309825483
1106	64-65	0.2601992986210746
1106	66-67	0.2592433608264173
1106	68-69	0.300938741283133
1106	70-71	0.2444883097280055
1106	72-73	0.14231578162549852
1106	74-75	0.18086121829755797
1106	76-77	0.21996193411084164
1106	78-79	0.2266219204359743
1106	80-81	0.18426030173503705
1106	82-83	0.1938577695146293
1106	84-85	0.19014381963852145
1106	86-87	0.1701765160746902
1106	88-89	0.18435663037136152
1106	90-91	0.2166049586500023
1106	92-93	0.21036597465548112
1106	94-95	0.1920013041122104
1106	96-97	0.19381305146765726
1106	98-99	0.23437015631612468
1106	100	0.24773543353860816
1107	1	0.021699313054774905
1107	2	0.07760322170837952
1107	3	0.07441684729347742
1107	4	0.05909058925296762
1107	5	0.013292202384064922
1107	6	0.02973789306329877
1107	7	0.014505672160545657
1107	8	0.018998044477868348
1107	9	0.015423271068712552
1107	10-11	0.06003188141509952
1107	12-13	0.07150391594266381
1107	14-15	0.1129429926064347
1107	16-17	0.06635003146764262
1107	18-19	0.07129468523895355
1107	20-21	0.07270017918581573
1107	22-23	0.09529537502785246
1107	24-25	0.10838876108572038
1107	26-27	0.08997210320676174
1107	28-29	0.11853178093791428
1107	30-31	0.09773772055562091
1107	32-33	0.08574933902891502
1107	34-35	0.09093865642643095
1107	36-37	0.14358518268247877
1107	38-39	0.1257713011514454
1107	40-41	0.09574478135326814
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2216	44-45	-0.6021886914521772
2216	46-47	-0.6052479399136601
2216	48-49	-0.6104318125769623
2216	50-51	-0.6850213561330705
2216	52-53	-0.6419698768912525
2216	54-55	-0.7880799208079665
2216	56-57	-0.7137525054673546
2216	58-59	-0.7063135749473091
2216	60-61	-0.7185890143792335
2216	62-63	-0.7650391547569129
2216	64-65	-0.6847182436132613
2216	66-67	-0.7064946708691977
2216	68-69	-0.6999454996124399
2216	70-71	-0.722887002454641
2216	72-73	-0.6989758589679482
2216	74-75	-0.6924018019301243
2216	76-77	-0.7121099585929898
2216	78-79	-0.6981794305316384
2216	80-81	-0.7327609500279806
2216	82-83	-0.653024751297913
2216	84-85	-0.7143032331987911
2216	86-87	-0.7411910120469116
2216	88-89	-0.7229686062364209
2216	90-91	-0.717217287728765
2216	92-93	-0.7559782835180187
2216	94-95	-0.7999062105862293
2216	96-97	-0.7773927755706183
2216	98-99	-0.8090861933644149
2216	100	-1.049017352923105
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	26.0
8	93.0
9	126.0
10	224.0
11	254.0
12	204.0
13	217.0
14	350.0
15	610.0
16	1233.0
17	2265.0
18	3892.0
19	6454.0
20	9076.0
21	13296.0
22	19672.0
23	24917.0
24	24839.0
25	22370.0
26	20611.0
27	22404.0
28	26814.0
29	32913.0
30	41020.0
31	51979.0
32	68132.0
33	91363.0
34	134041.0
35	215100.0
36	405248.0
37	967132.0
38	1361999.0
39	457577.0
40	1961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.639775847640344	18.78779182419337	13.239864523517353	22.332567804648928
2	13.589122877720577	22.75440829608663	38.637840298294975	25.018628527897818
3	20.83222143586075	27.019791237163943	31.370303686402462	20.777683640572846
4	11.53116910584121	17.456382316406565	40.323432657831425	30.689015919920802
5	11.420942566739503	41.04081813583099	34.92424406099811	12.613995236431391
6	27.827874570341805	37.300289310218304	19.76100006901342	15.110836050426467
7	24.85186463145509	34.565424075503735	22.943022184931525	17.639689108109653
8	23.902148042454446	32.73523164959295	22.157043519878307	21.20557678807431
9	25.578068976063022	18.215937198082024	21.793699353492148	34.41229447236281
10-11	23.722561148164587	25.436263718805325	28.55831280415211	22.282862328877982
12-13	27.95367008141173	23.175807241165998	27.931130182315016	20.939392495107253
14-15	24.960319351645264	24.48590164064649	27.929516643282764	22.624262364425483
16-17	20.985204591784555	27.810375403508875	29.698526367213677	21.505893637492886
18-19	20.34868826723781	29.34924481408555	29.99401501137421	20.30805190730243
20-21	21.677810015460185	27.80529896147663	28.17533310892729	22.341557914135894
22-23	21.178468759902376	26.97079737964697	27.21758340835299	24.633150452097656
24-25	22.40279792632928	27.587496015799772	27.367198290542277	22.642507767328667
26-27	23.054891108456633	25.07313055367723	30.249872654534837	21.6221056833313
28-29	21.530194295019623	27.456167569835245	29.561104753488348	21.45253338165678
30-31	21.635307523898074	26.685363867460875	29.445584058526308	22.233744550114743
32-33	21.408849533796396	27.79514571337325	29.406853176242848	21.389151576587505
34-35	22.452400755496082	27.303969507091807	29.006690462203288	21.236939275208826
36-37	22.998199642907455	25.318568874364118	29.716578738161303	21.96665274456712
38-39	23.067752547710025	26.25462267950643	29.3818036936753	21.29582107910825
40-41	22.541585641840125	26.402031008527448	30.059713130223383	20.996670219409044
42-43	22.47457881668387	26.455034992332532	28.194269039744864	22.87611715123874
44-45	21.995108706175333	26.59458561774926	28.929456044635288	22.480849631440115
46-47	22.835570990431673	27.883611214888308	27.592626840218003	21.688190954462012
48-49	22.573337346518922	27.579707761480243	27.525219458157885	22.321735433842946
50-51	21.35234960805526	27.318106511460954	28.4286290200463	22.90091486043749
52-53	22.170261547669316	28.266702115274008	26.74983436216214	22.813201974894543
54-55	22.062672178445602	26.919855995362855	27.89585294696268	23.121618879228865
56-57	22.602555076839977	26.44220791922891	28.229418732927346	22.72581827100377
58-59	23.043082744572686	26.860580246314424	27.737152076188842	22.359184932924048
60-61	22.628175092516685	27.05657620935736	29.206624833755445	21.10862386437051
62-63	22.219583987806473	27.30105930007627	28.246988389217222	22.232368322900033
64-65	21.049985781615796	28.31532565561274	27.82389460596333	22.810793956808137
66-67	22.772046308966083	25.969788851133295	27.819017101231108	23.439147738669515
68-69	22.93134055214232	26.592760719047593	27.35062891715631	23.12526981165378
70-71	22.884842358547928	26.990447292795572	27.450289939924303	22.674420408732193
72-73	22.490119656422223	25.79608758254307	28.979705575691376	22.73408718534333
74-75	21.547239882133024	29.374818470460024	27.014363486062255	22.063578161344697
76-77	22.328262845811537	26.408678844905936	26.850542589165894	24.412515720116634
78-79	22.174317511994623	27.1927027511765	27.48484342602804	23.14813631080084
80-81	22.991680467598705	26.862300437778018	27.795348116745274	22.350670977878003
82-83	22.688781813029763	25.780132834359442	28.608612773233443	22.92247257937735
84-85	21.71708752972793	27.232255616579103	28.139090164267106	22.91156668942586
86-87	21.845362849024095	27.99096219328167	27.94373513526798	22.219939822426262
88-89	22.018291119334386	27.534087329395796	26.935537482223104	23.512084069046715
90-91	22.495464714135498	25.81213143044952	27.255479330316764	24.436924525098227
92-93	23.21882667413363	27.37411168470355	27.06653390963983	22.34052773152299
94-95	22.59263699939331	26.682548855479528	28.128999715024182	22.59581443010298
96-97	21.5886573202656	26.284236490682204	28.878780646041335	23.248325543010857
98-99	21.797277000468053	27.65392414361227	27.54414078979721	23.00465806612247
100	21.76246911300136	26.523299359496615	26.3489122464188	25.36531928108323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	468.0
1	310.5
2	384.5
3	619.5
4	400.5
5	265.5
6	560.5
7	1284.5
8	2412.5
9	2993.0
10	2283.0
11	1538.0
12	1568.0
13	1846.0
14	2219.5
15	2608.5
16	2971.0
17	3406.5
18	3922.0
19	4431.0
20	5138.0
21	6165.0
22	7458.0
23	9485.0
24	11778.0
25	14588.5
26	18795.0
27	23521.0
28	28344.5
29	34389.5
30	42351.0
31	48790.5
32	56974.5
33	67456.0
34	74859.0
35	81453.0
36	90957.0
37	100640.0
38	104352.0
39	105898.5
40	105893.0
41	103849.5
42	103613.5
43	117598.0
44	162849.5
45	194979.0
46	177212.5
47	197263.0
48	327055.5
49	329062.0
50	222797.5
51	171428.5
52	121511.0
53	103048.0
54	94990.0
55	84391.5
56	75588.0
57	66344.5
58	57928.5
59	52962.0
60	44635.5
61	34576.5
62	27414.5
63	21496.0
64	15392.0
65	10492.5
66	7536.5
67	5551.5
68	4162.5
69	3269.0
70	2820.0
71	2297.5
72	1899.5
73	1871.5
74	1501.5
75	1105.0
76	848.5
77	564.0
78	357.5
79	211.5
80	144.0
81	102.0
82	54.0
83	25.5
84	19.5
85	19.0
86	14.0
87	5.0
88	3.0
89	2.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006429332451596311
2	0.09112771980621645
3	3.227078064507801E-4
4	0.0
5	0.03969306019344595
6	0.005212972258051063
7	4.964735483858156E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.833564193533332E-4
24-25	0.0
26-27	0.0
28-29	4.964735483858156E-5
30-31	3.7235516128936165E-5
32-33	0.001874187645156454
34-35	0.001625950870963546
36-37	0.0011046536451584396
38-39	9.805352580619857E-4
40-41	0.0017004219032214182
42-43	7.32298483869078E-4
44-45	7.447103225787234E-4
46-47	4.220025161279432E-4
48-49	6.08180096772624E-4
50-51	9.432997419330496E-4
52-53	0.0011170654838680851
54-55	0.001725245580640709
56-57	9.929470967716312E-4
58-59	0.002618897967735177
60-61	0.0020976007419300706
62-63	0.0011046536451584396
64-65	0.004381379064504822
66-67	0.004071083096763688
68-69	0.003450491161281418
70-71	0.0019238349999950353
72-73	0.005262619612889645
74-75	0.0035249621935392905
76-77	0.003326372774184964
78-79	0.0011046536451584396
80-81	1.241183870964539E-5
82-83	2.854722903218439E-4
84-85	8.688287096751771E-5
86-87	2.482367741929078E-5
88-89	2.23413096773617E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	4.095906774182978E-4
98-99	5.833564193533332E-4
100	4.46826193547234E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4028412.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.111846734380315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34760453978014	35.75360223562639
2	15.485747162755755	15.520387767747623
3	5.760669378917373	8.660333430112358
4	2.741195186843575	5.494654122885049
5	1.491653430393074	3.737475404233518
6	0.8775578345383392	2.638562622296396
7	0.5726410161281041	2.0087269183821954
8	0.38903734580407145	1.5596303877506994
9	0.2582849784702427	1.1648823529404166
>10	1.002485084404405	8.045599118368823
>50	0.042307468019974405	1.4852179334999274
>100	0.0264746658777063	2.4529557075850583
>500	0.0020566932950117456	0.7066421152612353
>1k	0.001142607386115054	1.066681464392084
>5k	3.046953029640144E-4	1.0569094040254803
>10k+	8.379120831510394E-4	8.647739014892966
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	61523	1.5272271058670266	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	44125	1.0953447661262055	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	19113	0.47445494651490466	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	16448	0.4082998461924947	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	15141	0.37585529980548166	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	14366	0.3566169498055313	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	12606	0.31292727754757954	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11387	0.2826672147734641	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10833	0.26891489748317704	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10643	0.26419839877351176	No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA	10361	0.2571981217412717	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	9603	0.23838177425744936	No Hit
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG	6546	0.16249579238667744	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	5778	0.1434312081286621	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5761	0.1430092056125342	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.964735483858155E-5
7	0.0	0.0	0.0	0.0	4.964735483858155E-5
8	0.0	0.0	0.0	0.0	4.964735483858155E-5
9	0.0	0.0	0.0	0.0	4.964735483858155E-5
10-11	0.0	0.0	0.0	3.7235516128936165E-5	4.964735483858155E-5
12-13	0.0	0.0	0.0	9.92947096771631E-5	1.117065483868085E-4
14-15	0.0	0.0	0.0	1.6135390322539005E-4	1.241183870964539E-4
16-17	0.0	0.0	0.0	2.358249354832624E-4	1.241183870964539E-4
18-19	0.0	0.0	0.0	3.475314838700709E-4	1.241183870964539E-4
20-21	0.0	0.0	0.0	5.337090645147517E-4	1.241183870964539E-4
22-23	0.0	0.0	0.0	9.184760645137588E-4	1.241183870964539E-4
24-25	0.0	0.0	0.0	0.0016507745483828368	1.241183870964539E-4
26-27	0.0	0.0	0.0	0.002643721645154468	1.241183870964539E-4
28-29	0.0	0.0	0.0	0.006764452096756737	1.241183870964539E-4
30-31	0.0	0.0	0.0	0.015489974709637446	1.241183870964539E-4
32-33	0.0	0.0	0.0	0.035224798257973616	1.241183870964539E-4
34-35	0.0	0.0	0.0	0.0708219516772366	1.241183870964539E-4
36-37	0.0	0.0	0.0	0.12605463393515856	1.241183870964539E-4
38-39	0.0	0.0	0.0	0.20264809061238026	1.241183870964539E-4
40-41	0.0	0.0	0.0	0.27901813419282834	1.241183870964539E-4
42-43	0.0	0.0	0.0	0.34485052670878746	1.241183870964539E-4
44-45	0.0	0.0	0.0	0.42165498464407314	1.241183870964539E-4
46-47	0.0	0.0	0.0	0.515984958837378	1.365302258060993E-4
48-49	0.0	0.0	0.0	0.6147211357726072	1.4894206451574466E-4
50-51	0.0	0.0	0.0	0.7183599889981462	1.4894206451574466E-4
52-53	0.0	0.0	0.0	0.8184987037075652	1.7376574193503545E-4
54-55	0.0	0.0	0.0	0.9229939738040698	1.7376574193503545E-4
56-57	0.0	0.0	0.0	1.0485397223521327	1.7376574193503545E-4
58-59	0.0	0.0	0.0	1.1866214279969376	1.7376574193503545E-4
60-61	0.0	0.0	0.0	1.3317158225126924	1.7376574193503545E-4
62-63	0.0	0.0	0.0	1.4859825658348749	1.7376574193503545E-4
64-65	0.0	0.0	0.0	1.6558509904150818	1.7376574193503545E-4
66-67	0.0	0.0	0.0	1.8249126454791615	1.8617758064468082E-4
68-69	0.0	0.0	0.0	2.0011111078012873	1.985894193543262E-4
70-71	0.0	0.0	0.0	2.1900813521556386	1.985894193543262E-4
72-73	0.0	0.0	0.0	2.368638063832597	2.110012580639716E-4
74-75	0.0	0.0	0.0	2.5473437175740714	2.23413096773617E-4
76-77	0.0	0.0	0.0	2.735134837251006	2.23413096773617E-4
78-79	0.0	0.0	0.0	2.963140314347192	2.23413096773617E-4
80-81	0.0	0.0	0.0	3.19807159744336	2.23413096773617E-4
82-83	0.0	0.0	0.0	3.4267845493459954	2.23413096773617E-4
84-85	0.0	0.0	0.0	3.6621626586357108	2.23413096773617E-4
86-87	0.0	0.0	0.0	3.901028494602836	2.23413096773617E-4
88	0.0	0.0	0.0	4.091115804441055	2.23413096773617E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	12060	0.0	21.900618	12-13
TACCTGG	4165	0.0	21.5803	2
GTACCCG	505	0.0	21.433968	1
CTAACGC	495	0.0	20.893646	3
CATGGGG	7065	0.0	20.095161	4
AGTACTT	12875	0.0	19.857244	12-13
GTACTTT	13495	0.0	19.397675	14-15
TAACGCC	575	0.0	18.80428	4
ACCTGGG	4690	0.0	18.643904	3
GTACCTG	5055	0.0	18.247398	1
TGCGCGA	945	0.0	17.40705	94
ACTTTTT	15335	0.0	17.131605	16-17
ATGGGGG	4335	0.0	17.02579	5
TAGTACT	2000	0.0	16.688799	4
GTCCTAC	1425	0.0	16.51282	1
TACTTTT	15555	0.0	16.45111	14-15
GAGTACG	1350	0.0	16.384386	1
TCTAACG	580	0.0	16.227108	2
GGGCTAT	1870	0.0	15.833701	6
CATGGGT	2025	0.0	15.786309	4
>>END_MODULE