##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146559_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7679085
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094630024280235	33.0	31.0	34.0	30.0	34.0
2	32.21408787635506	33.0	31.0	34.0	30.0	34.0
3	32.412764932280346	34.0	31.0	34.0	31.0	34.0
4	35.63811469725885	37.0	35.0	37.0	35.0	37.0
5	35.76628439456003	37.0	35.0	37.0	35.0	37.0
6	36.01604839639098	37.0	37.0	37.0	35.0	37.0
7	36.070996088726716	37.0	37.0	37.0	35.0	37.0
8	36.03886530752036	37.0	37.0	37.0	35.0	37.0
9	37.769617994852254	39.0	38.0	39.0	35.0	39.0
10-11	37.74010210851944	39.0	38.5	39.0	35.0	39.0
12-13	37.75956061171351	39.0	38.0	39.0	35.0	39.0
14-15	38.94683858819117	40.0	39.0	41.0	36.0	41.0
16-17	39.17623414768816	40.5	39.0	41.0	36.5	41.0
18-19	39.324557613309395	41.0	40.0	41.0	37.0	41.0
20-21	39.303578030455455	41.0	39.5	41.0	37.0	41.0
22-23	39.212908048289606	41.0	39.0	41.0	36.5	41.0
24-25	39.09496815050231	40.5	39.0	41.0	36.0	41.0
26-27	38.774475604840944	40.5	38.5	41.0	35.0	41.0
28-29	38.739919860243766	40.0	38.0	41.0	35.5	41.0
30-31	38.82596676557168	40.0	39.0	41.0	35.0	41.0
32-33	38.65876546489588	40.0	38.0	41.0	35.0	41.0
34-35	38.19251447275294	40.0	38.0	41.0	34.0	41.0
36-37	37.95341945036421	40.0	37.5	41.0	33.5	41.0
38-39	37.75682044149791	40.0	37.5	41.0	33.0	41.0
40-41	38.00955973270253	40.0	38.0	41.0	33.0	41.0
42-43	37.781064019997174	40.0	38.0	41.0	33.0	41.0
44-45	37.484043671869756	40.0	37.0	41.0	31.5	41.0
46-47	37.10857224526099	39.0	37.0	41.0	31.0	41.0
48-49	36.73754841885459	39.0	36.0	40.0	29.5	41.0
50-51	36.237772403873635	39.0	35.0	40.0	28.0	41.0
52-53	36.29726933612533	39.0	35.0	40.0	28.5	41.0
54-55	36.001860833680055	38.5	34.5	40.0	28.0	41.0
56-57	34.946160512613154	38.0	33.0	40.0	25.0	41.0
58-59	34.54923138629147	38.0	32.0	40.0	25.0	41.0
60-61	33.75281651394665	36.5	31.0	39.0	22.0	41.0
62-63	34.06903941029432	36.5	31.5	39.0	24.0	41.0
64-65	33.891957896025374	36.5	31.5	39.0	24.5	40.5
66-67	32.93344747453635	36.0	30.5	39.0	22.0	39.5
68-69	32.13963655826182	35.0	29.5	37.0	21.0	39.0
70-71	31.247714278458957	34.0	29.0	36.5	20.5	38.5
72-73	30.96344323575009	34.0	28.5	36.0	20.5	37.0
74-75	31.055410638116392	34.0	29.0	35.5	20.0	37.0
76-77	30.866325480184162	34.0	29.0	35.0	20.0	36.5
78-79	30.31237393517587	34.0	28.5	35.0	20.0	36.0
80-81	29.26145465247487	32.0	26.0	35.0	16.0	36.0
82-83	28.99848536642061	32.5	25.5	35.0	15.0	35.5
84-85	29.004693918611398	32.0	26.0	35.0	18.0	35.0
86-87	29.689327244066188	33.5	28.0	35.0	18.0	35.0
88-89	29.267137556102064	33.0	26.5	35.0	17.5	35.0
90-91	28.588632174275972	32.0	25.0	35.0	13.5	35.0
92-93	28.17108431538393	32.0	25.0	35.0	11.0	35.0
94-95	28.104349411420763	31.5	24.5	34.5	12.5	35.0
96-97	28.21327684222795	32.0	25.0	34.5	11.5	35.0
98-99	28.648114521456655	32.0	25.0	35.0	13.0	35.0
100	27.226719980310154	31.0	24.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18334727913565274
1101	2	0.23920678781765048
1101	3	0.2979006981743453
1101	4	0.22099781305573885
1101	5	0.17141209516964295
1101	6	0.15254174633236062
1101	7	0.14252165707846132
1101	8	0.17713085986613208
1101	9	0.2487459963486458
1101	10-11	0.2546726078877555
1101	12-13	0.21280107730678566
1101	14-15	0.3709756295647111
1101	16-17	0.330053085739209
1101	18-19	0.29870015467603395
1101	20-21	0.2768417060293089
1101	22-23	0.36346562965876217
1101	24-25	0.40338822016263975
1101	26-27	0.42126906826641886
1101	28-29	0.43513820248274726
1101	30-31	0.39315669788650354
1101	32-33	0.41273394592752766
1101	34-35	0.4791084615922401
1101	36-37	0.5142369823849506
1101	38-39	0.5061869714644232
1101	40-41	0.44559016738976
1101	42-43	0.43857657311898635
1101	44-45	0.469676406574564
1101	46-47	0.5569654785532805
1101	48-49	0.5803314049506909
1101	50-51	0.6125144081387361
1101	52-53	0.5531214147080163
1101	54-55	0.6062305925856819
1101	56-57	0.6416784972198215
1101	58-59	0.7216076114461174
1101	60-61	0.774205316426773
1101	62-63	0.6622763601428048
1101	64-65	0.6529152521391595
1101	66-67	0.6524598153264947
1101	68-69	0.5873289353496673
1101	70-71	0.6791358887824472
1101	72-73	0.6485329216058773
1101	74-75	0.5706889383622702
1101	76-77	0.5197190488339061
1101	78-79	0.5364350042940522
1101	80-81	0.6018798214864489
1101	82-83	0.6347982586022596
1101	84-85	0.6271448030586022
1101	86-87	0.5763787835318581
1101	88-89	0.5429791430845263
1101	90-91	0.6250651946682169
1101	92-93	0.6912534930132814
1101	94-95	0.6921477056824727
1101	96-97	0.5547546016055911
1101	98-99	0.46975113875167196
1101	100	0.4061232109545436
1102	1	0.20703035543028392
1102	2	0.3005960586346532
1102	3	0.2689870291294483
1102	4	0.2220381884835021
1102	5	0.1966818433957087
1102	6	0.19525629445027448
1102	7	0.2007501211204712
1102	8	0.24446778197130214
1102	9	0.29494179758459893
1102	10-11	0.3068625723992753
1102	12-13	0.2442989586606501
1102	14-15	0.37708362067206735
1102	16-17	0.3481052142202614
1102	18-19	0.3204345049822379
1102	20-21	0.3410343954707926
1102	22-23	0.36370668966020503
1102	24-25	0.43730039875471505
1102	26-27	0.4193272194184203
1102	28-29	0.4613314908237669
1102	30-31	0.44048303304985126
1102	32-33	0.4275509326429159
1102	34-35	0.5039789299421358
1102	36-37	0.5482427681901427
1102	38-39	0.49942403416348924
1102	40-41	0.4602384055110136
1102	42-43	0.41984108713115376
1102	44-45	0.4806990832046836
1102	46-47	0.5709958390793588
1102	48-49	0.646869397073992
1102	50-51	0.7003175683125704
1102	52-53	0.6664976628177186
1102	54-55	0.6581467292459422
1102	56-57	0.7281082681051458
1102	58-59	0.7298989982801132
1102	60-61	0.7043697405370608
1102	62-63	0.7048503173979057
1102	64-65	0.6435296950185645
1102	66-67	0.6007227784724947
1102	68-69	0.652125281514806
1102	70-71	0.7106059826345259
1102	72-73	0.7359976159086656
1102	74-75	0.5393637665514213
1102	76-77	0.5748785940289309
1102	78-79	0.4543533764509746
1102	80-81	0.5967621943895907
1102	82-83	0.5728097535294872
1102	84-85	0.6369344936124364
1102	86-87	0.5905351184614958
1102	88-89	0.4959111926362887
1102	90-91	0.5804369909351053
1102	92-93	0.6090655890066365
1102	94-95	0.6873091657249475
1102	96-97	0.5541508840222811
1102	98-99	0.5350460877415486
1102	100	0.44601486536002
1103	1	0.19005415391102787
1103	2	0.18541240965487304
1103	3	0.23184624893860217
1103	4	0.12727441977313703
1103	5	0.08784259802338568
1103	6	0.10182169528589924
1103	7	0.09158807160928006
1103	8	0.13822014728499
1103	9	0.19551894787564805
1103	10-11	0.200985127067014
1103	12-13	0.174389886325244
1103	14-15	0.30682912621190894
1103	16-17	0.2612730195479429
1103	18-19	0.22049920841383397
1103	20-21	0.22537618641312918
1103	22-23	0.26304914544196834
1103	24-25	0.27127188422274173
1103	26-27	0.31497172872995094
1103	28-29	0.29788594926015577
1103	30-31	0.3343285950587713
1103	32-33	0.3348481661428977
1103	34-35	0.3957687151524567
1103	36-37	0.4646712304394853
1103	38-39	0.42063312171065803
1103	40-41	0.40376939264059075
1103	42-43	0.4213205152759443
1103	44-45	0.46183801557585014
1103	46-47	0.5326836957582657
1103	48-49	0.5274767604308508
1103	50-51	0.5560035221674795
1103	52-53	0.5974278955037704
1103	54-55	0.6516579188896614
1103	56-57	0.7587215872090667
1103	58-59	0.7958012136937498
1103	60-61	0.7424902294049787
1103	62-63	0.6420409905941185
1103	64-65	0.6589105824100088
1103	66-67	0.6793448021548727
1103	68-69	0.7110478289138271
1103	70-71	0.7140471352597118
1103	72-73	0.734723160396058
1103	74-75	0.5740821456853347
1103	76-77	0.609662025973833
1103	78-79	0.5816070542597487
1103	80-81	0.7007248777381356
1103	82-83	0.5852173714586257
1103	84-85	0.6084337942119582
1103	86-87	0.536370318631743
1103	88-89	0.4779208741732148
1103	90-91	0.5994213141091151
1103	92-93	0.755117242435901
1103	94-95	0.6814848484677718
1103	96-97	0.5169191887974698
1103	98-99	0.3528459272144566
1103	100	0.47534796368224264
1104	1	0.15154259419942662
1104	2	0.21592249341965442
1104	3	0.22229161764339267
1104	4	0.16206146757835427
1104	5	0.13066530977186375
1104	6	0.13221474798045563
1104	7	0.12465660152428626
1104	8	0.15167460866433657
1104	9	0.19092698218425852
1104	10-11	0.19134498770942798
1104	12-13	0.18625810842645762
1104	14-15	0.30262543983980095
1104	16-17	0.26203391186074043
1104	18-19	0.24875460713820274
1104	20-21	0.24343345427673313
1104	22-23	0.2794044468980559
1104	24-25	0.30358277691815516
1104	26-27	0.3113949333338013
1104	28-29	0.33833410858871105
1104	30-31	0.33451501101041714
1104	32-33	0.3233665767787244
1104	34-35	0.3534529127866364
1104	36-37	0.3711652137137875
1104	38-39	0.37893621535503286
1104	40-41	0.3806561465013658
1104	42-43	0.4048806385034922
1104	44-45	0.38751907910732086
1104	46-47	0.41712940992171355
1104	48-49	0.4954751396609325
1104	50-51	0.5125734024131745
1104	52-53	0.4469960093569654
1104	54-55	0.4994047700248032
1104	56-57	0.502372508882047
1104	58-59	0.4835290535617389
1104	60-61	0.6607021294242088
1104	62-63	0.6192449161679932
1104	64-65	0.5446782831975767
1104	66-67	0.5404864665141034
1104	68-69	0.529754318454188
1104	70-71	0.5462146629905362
1104	72-73	0.5054731993524797
1104	74-75	0.47730173362555917
1104	76-77	0.46638772810219464
1104	78-79	0.47888475509338235
1104	80-81	0.49837112365056413
1104	82-83	0.5193382063812741
1104	84-85	0.4731853474148977
1104	86-87	0.46226081219486304
1104	88-89	0.4416239580485133
1104	90-91	0.4543027870285137
1104	92-93	0.48928366960258174
1104	94-95	0.3374707707036322
1104	96-97	0.42510157559094
1104	98-99	0.3031150225334436
1104	100	0.3864546995589144
1105	1	0.14867153742559935
1105	2	0.20135244679374154
1105	3	0.2193675288495811
1105	4	0.19018278251849807
1105	5	0.13798079196191537
1105	6	0.14716659404638222
1105	7	0.10138714465066556
1105	8	0.14570250978249533
1105	9	0.1709619223905392
1105	10-11	0.2253504234503083
1105	12-13	0.19660883898851012
1105	14-15	0.34334996938942197
1105	16-17	0.2782477406977506
1105	18-19	0.23019568261768342
1105	20-21	0.26647760053796077
1105	22-23	0.28882826520751337
1105	24-25	0.32742909080122473
1105	26-27	0.3118012178870586
1105	28-29	0.3383327317870837
1105	30-31	0.3553149752007627
1105	32-33	0.4017277580060892
1105	34-35	0.35510656588777323
1105	36-37	0.44080338237990446
1105	38-39	0.4654303741758099
1105	40-41	0.4057561183084957
1105	42-43	0.4163855633640665
1105	44-45	0.49158617122672865
1105	46-47	0.5808893281764824
1105	48-49	0.588548232970453
1105	50-51	0.5291659020114281
1105	52-53	0.5030724624832459
1105	54-55	0.5838785799554458
1105	56-57	0.5502756043327821
1105	58-59	0.6587785917660085
1105	60-61	0.7660664065249563
1105	62-63	0.6107481519079556
1105	64-65	0.5914793152736877
1105	66-67	0.6356002771443983
1105	68-69	0.665021404887078
1105	70-71	0.6436397530438853
1105	72-73	0.6627819695202675
1105	74-75	0.5534194509144541
1105	76-77	0.48338080378718473
1105	78-79	0.43358250651926866
1105	80-81	0.48849744798964423
1105	82-83	0.47762634540087845
1105	84-85	0.50487605029344
1105	86-87	0.4472241944861608
1105	88-89	0.45839352988736337
1105	90-91	0.5353583690314636
1105	92-93	0.5257530725238801
1105	94-95	0.5535635956021494
1105	96-97	0.3564089403049948
1105	98-99	0.394282132969348
1105	100	0.3684537913638479
1106	1	0.13257675452935302
1106	2	0.13846631797678555
1106	3	0.15491907613925093
1106	4	0.16734099336532893
1106	5	0.1164279206671992
1106	6	0.11630315353473009
1106	7	0.09806591032300105
1106	8	0.14343678719664155
1106	9	0.17293683755490008
1106	10-11	0.18725266028866372
1106	12-13	0.17408723017679506
1106	14-15	0.2609053284877234
1106	16-17	0.2114024473759244
1106	18-19	0.17515791527402058
1106	20-21	0.17222101220622932
1106	22-23	0.19718423779647765
1106	24-25	0.23594857953969495
1106	26-27	0.26153967866693506
1106	28-29	0.26222982324486566
1106	30-31	0.2732200168426999
1106	32-33	0.30082998091299373
1106	34-35	0.29479107176815944
1106	36-37	0.32818509916968
1106	38-39	0.32735771503528355
1106	40-41	0.27630591085061695
1106	42-43	0.31993863690788515
1106	44-45	0.39540038192296834
1106	46-47	0.3914333350807766
1106	48-49	0.3853123068072506
1106	50-51	0.42719813356808345
1106	52-53	0.4794719030635761
1106	54-55	0.5934349534537446
1106	56-57	0.528695027858717
1106	58-59	0.584042946106436
1106	60-61	0.570703444241154
1106	62-63	0.5690625874130504
1106	64-65	0.49471959049071046
1106	66-67	0.546369444545455
1106	68-69	0.53509392626448
1106	70-71	0.523312319452863
1106	72-73	0.5107341482346293
1106	74-75	0.4597607799134167
1106	76-77	0.4651711318689955
1106	78-79	0.4275861903902154
1106	80-81	0.45735554650833166
1106	82-83	0.48299530663816626
1106	84-85	0.5551423341709736
1106	86-87	0.39443483883933794
1106	88-89	0.279492654209772
1106	90-91	0.37384491743645043
1106	92-93	0.3169040485826464
1106	94-95	0.4000765658794663
1106	96-97	0.38176987104801796
1106	98-99	0.36552065325782834
1106	100	0.3089908488317832
1107	1	0.0867179088058947
1107	2	0.10403948811818964
1107	3	0.12228221979383136
1107	4	0.10660625603193097
1107	5	0.06905838182620982
1107	6	0.049874648664641086
1107	7	0.04888010109964824
1107	8	0.0692628207304935
1107	9	0.10091233395901611
1107	10-11	0.11182683491430367
1107	12-13	0.0997531968287646
1107	14-15	0.1595499202939692
1107	16-17	0.13279422107400052
1107	18-19	0.1021175303115669
1107	20-21	0.1124884305374323
1107	22-23	0.12376772023446847
1107	24-25	0.20298504056617617
1107	26-27	0.1682366588915869
1107	28-29	0.17427139835322691
1107	30-31	0.19328113463593866
1107	32-33	0.21861972710763666
1107	34-35	0.18784869440247576
1107	36-37	0.2286917527027299
1107	38-39	0.2375071949115224
1107	40-41	0.22081075644465642
1107	42-43	0.24397223574130322
1107	44-45	0.27606226421323754
1107	46-47	0.314199172849996
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2209	3	0.025210757582172505
2209	4	-0.028313549515921466
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2209	6	0.028079321701106608
2209	7	0.004939215331347668
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2210	4	-0.08917478938937506
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2210	12-13	0.020170555881051655
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2210	16-17	0.003497667246570302
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2210	22-23	0.014526826941704485
2210	24-25	-0.010544168188275194
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2210	28-29	0.06572392954022632
2210	30-31	0.11600358042429093
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2211	9	-0.0481349179146946
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2212	98-99	0.11879186647883344
2212	100	0.21245601811506631
2213	1	-0.11746268534820103
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2213	96-97	-0.08107172057370704
2213	98-99	-0.11583362914766226
2213	100	-0.17106301787201872
2214	1	-0.15497499192067465
2214	2	-0.22476669349141787
2214	3	-0.2118774361452438
2214	4	-0.19949596782305434
2214	5	-0.1637644559737481
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2214	7	-0.14160256028583973
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2214	9	-0.21425300210147213
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2214	16-17	-0.26872478560359525
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2214	22-23	-0.22364796582139945
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2214	34-35	-0.25671380946408817
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2214	78-79	-0.47811928852220476
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2214	82-83	-0.4353840556494042
2214	84-85	-0.4835116527417149
2214	86-87	-0.44473334599260284
2214	88-89	-0.49297363719557197
2214	90-91	-0.4733015411464194
2214	92-93	-0.5424821411804182
2214	94-95	-0.6724943626299442
2214	96-97	-0.583880933552166
2214	98-99	-0.4421910217424987
2214	100	-0.40742142236069867
2215	1	-0.26256398845661266
2215	2	-0.36700307367763685
2215	3	-0.3446263372128655
2215	4	-0.27450181675711605
2215	5	-0.25477161732168696
2215	6	-0.22578918422767913
2215	7	-0.24727266571356665
2215	8	-0.21709797553514676
2215	9	-0.29433826003303665
2215	10-11	-0.2739027376202472
2215	12-13	-0.3190366157293454
2215	14-15	-0.472295461750349
2215	16-17	-0.4139157252956025
2215	18-19	-0.4376339861268477
2215	20-21	-0.4778086653303788
2215	22-23	-0.4466063327965628
2215	24-25	-0.4514821591982212
2215	26-27	-0.4968252524038732
2215	28-29	-0.4957133263678344
2215	30-31	-0.5087437810954683
2215	32-33	-0.6101763915904712
2215	34-35	-0.6922091324593111
2215	36-37	-0.6892635823877455
2215	38-39	-0.7815201839795591
2215	40-41	-0.7299696150429256
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2215	50-51	-1.1231072619915707
2215	52-53	-1.0822452876280124
2215	54-55	-1.2021959354553005
2215	56-57	-1.435408579396082
2215	58-59	-1.4935477205888645
2215	60-61	-1.6640423722765547
2215	62-63	-1.4654029845031076
2215	64-65	-1.341336095302971
2215	66-67	-1.4686542351183398
2215	68-69	-1.4801349155682075
2215	70-71	-1.5174266995069061
2215	72-73	-1.3954192819568547
2215	74-75	-1.314306770705251
2215	76-77	-1.2651029221362755
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2215	80-81	-1.449425674480576
2215	82-83	-1.4266075326769574
2215	84-85	-1.3745501920446515
2215	86-87	-1.1966716723343538
2215	88-89	-1.3013672462786552
2215	90-91	-1.4029713135736088
2215	92-93	-1.5426698947183901
2215	94-95	-1.6053317169651642
2215	96-97	-1.4863679211276946
2215	98-99	-1.4274152614881963
2215	100	-1.3507818262707616
2216	1	-0.22313304336447004
2216	2	-0.3224678019537066
2216	3	-0.31901910017305113
2216	4	-0.23351862910960364
2216	5	-0.20160403640828406
2216	6	-0.22336989958964892
2216	7	-0.23169491515638185
2216	8	-0.18625888518182876
2216	9	-0.26815988620795395
2216	10-11	-0.2561927789162013
2216	12-13	-0.2620268159648447
2216	14-15	-0.44564101278605506
2216	16-17	-0.402868894253686
2216	18-19	-0.46365569306571075
2216	20-21	-0.4596787841912189
2216	22-23	-0.517557708450127
2216	24-25	-0.6485273803391323
2216	26-27	-0.6918237406812366
2216	28-29	-0.8180696056891037
2216	30-31	-0.9582155269163763
2216	32-33	-0.9985731610423443
2216	34-35	-1.3427968057202406
2216	36-37	-1.3885382641561606
2216	38-39	-1.515297277057229
2216	40-41	-1.515948159032959
2216	42-43	-1.7327215453560356
2216	44-45	-2.055994220201846
2216	46-47	-2.3861609730007487
2216	48-49	-2.5254224706598194
2216	50-51	-2.5092280372084588
2216	52-53	-2.6146546382223192
2216	54-55	-2.8823386198131544
2216	56-57	-3.422596253653065
2216	58-59	-3.6502057218850794
2216	60-61	-3.7528732122320108
2216	62-63	-3.4692216349307543
2216	64-65	-3.2041204043164306
2216	66-67	-3.556585359861259
2216	68-69	-3.6211116382662283
2216	70-71	-3.5583471759753174
2216	72-73	-3.2056186411248753
2216	74-75	-2.9626334231621243
2216	76-77	-2.799596235753498
2216	78-79	-2.8301415910876457
2216	80-81	-3.0594304678957442
2216	82-83	-3.113352569495486
2216	84-85	-2.9903670709124732
2216	86-87	-2.710595073882608
2216	88-89	-2.863260544964536
2216	90-91	-3.0074224137595422
2216	92-93	-3.1788886764651814
2216	94-95	-3.1554712628413952
2216	96-97	-2.755494340911852
2216	98-99	-2.6469638565026905
2216	100	-2.707868207044502
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	228.0
1	134.0
2	117.0
3	87.0
4	72.0
5	124.0
6	223.0
7	949.0
8	2715.0
9	3405.0
10	3371.0
11	4047.0
12	5764.0
13	7279.0
14	8669.0
15	9923.0
16	11316.0
17	13414.0
18	15875.0
19	19875.0
20	25120.0
21	31358.0
22	39232.0
23	50073.0
24	63460.0
25	79603.0
26	97539.0
27	116725.0
28	142146.0
29	177719.0
30	225007.0
31	287637.0
32	382973.0
33	525008.0
34	744087.0
35	1032356.0
36	1288224.0
37	1329605.0
38	780253.0
39	153152.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.12629166819774	14.822595394701693	12.409578991287633	17.641533945812938
2	11.925270870822686	30.977049450196663	28.090339373696573	29.007340305284078
3	41.59673223112252	18.745884422068666	27.23741570879629	12.419967638012519
4	9.53442013696789	12.101864788750003	49.17821610419812	29.18549897008399
5	9.737586258274177	44.76942484759258	34.036631960181836	11.456356933951403
6	24.374238905769534	37.7366115791818	14.851414870104415	23.037734644944248
7	25.10836627351159	34.80036448313211	21.041920639098276	19.049348604258025
8	20.208376174186665	15.992992484449992	19.93901763248128	43.85961370888206
9	14.749864458445233	30.881152294009066	31.507059295897598	22.861923951648098
10-11	28.324122531983846	17.44751080454022	26.990421938702752	27.237944724773183
12-13	40.62562572698283	18.850466724364054	20.441889656906753	20.08201789174636
14-15	25.363858437882758	23.87197746771341	36.62363307464295	14.14053101976088
16-17	18.41165169399261	26.147090120809185	32.70174103773827	22.73951714745993
18-19	15.40682797712317	41.583431807323464	23.478686738234742	19.53105347731862
20-21	23.998661978425215	24.781594740952695	17.756252164322177	33.46349111629991
22-23	17.81113092233173	27.731549546453422	23.161150218791974	31.29616931242288
24-25	31.004443755675158	25.38386931991883	20.444258360977255	23.16742856342876
26-27	27.42795030069056	16.71481527110597	35.76169820299329	20.095536225210186
28-29	17.351532730152407	38.733803216862206	25.26490122932403	18.649762823661362
30-31	22.009220885871077	24.172210895612125	22.01077070214296	31.807797516373842
32-33	24.727921789113747	25.7678184317579	26.385794094070043	23.11846568505831
34-35	32.55807789772799	20.77097789776706	25.823716904705375	20.847227299799574
36-37	26.7280362314532	17.87360788086076	35.36508003079332	20.033275856892715
38-39	21.037846953855148	27.842598330781758	32.16529996459834	18.954254750764758
40-41	20.84158460977389	32.2360763780348	25.021732051682815	21.900606960508494
42-43	17.631410506067187	32.23254383563178	17.700296279816527	32.43574937848451
44-45	24.016716399246178	20.40802316822855	20.96171725734078	34.61354317518449
46-47	31.48676947622669	29.192839961035432	22.69671101634839	16.623679546389486
48-49	28.027072882351806	25.79007678062124	25.3618149101332	20.82103542689375
50-51	12.72929849101799	28.189798667789738	38.58615297517145	20.494749866020822
52-53	22.990532402552656	34.59041047532081	20.32994603156821	22.089111090558326
54-55	17.077730091723843	23.684779094892512	27.22130478188662	32.01618603149702
56-57	27.637614305402646	18.206556325874928	26.514860467712705	27.640968901009728
58-59	38.363251267855645	20.058417858671458	20.741355624430344	20.83697524904255
60-61	24.969385886085792	24.151793635338745	36.06966625926012	14.809154219315342
62-63	19.274203008503633	25.588303741770137	32.10333482901575	23.034158420710472
64-65	16.169493287381016	39.90137647216527	23.81701464612864	20.112115594325072
66-67	23.648687466495403	24.63331720118783	18.748772990749963	32.969222341566805
68-69	17.9168349945082	27.753562307834308	23.69378789167734	30.63581480598015
70-71	30.244898605246068	25.47319647105884	20.509193997639528	23.772710926055563
72-73	26.582405288997812	17.491588571986874	34.764341602820366	21.161664536194948
74-75	17.76573274411923	37.87555934162144	25.090213448872902	19.26849446538643
76-77	22.32487019007957	23.646571790267945	22.486650983845696	31.541907035806794
78-79	24.58005899428328	25.30603976528652	26.808013243588025	23.305887996842173
80-81	31.762578880085986	21.232012897657416	25.776802130067665	21.228606092188937
82-83	26.228277209107702	18.379373006880968	34.35549993701226	21.03684984699907
84-85	20.992215209801394	27.639183787043343	31.4802004955722	19.888400507583068
86-87	21.224636360077696	31.456466153165458	25.044103669171253	22.274793817585596
88-89	18.413117968433347	31.30479706940888	18.47006969747911	31.812015264678667
90-91	24.05007308996413	20.45510072072573	21.754338908553166	33.74048728075697
92-93	30.756260922296107	28.774944195737966	23.239246077417953	17.229548804547974
94-95	27.368382176113315	25.773638942351912	25.259779477304043	21.598199404230726
96-97	13.359355286748448	27.76275857109788	37.31193659791672	21.56594954423695
98-99	23.29562844138907	33.673156951929144	20.601755042887362	22.429459563794417
100	20.75662764981593	15.395668590284508	22.147679244648916	41.70002451525065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	397.0
1	303.5
2	209.5
3	215.0
4	208.0
5	172.0
6	149.5
7	140.5
8	144.5
9	150.0
10	144.5
11	160.0
12	183.0
13	210.5
14	215.5
15	233.5
16	276.5
17	332.5
18	404.0
19	514.5
20	701.0
21	986.5
22	1393.0
23	2018.0
24	2903.0
25	3919.0
26	5508.5
27	7645.5
28	10060.5
29	12865.0
30	15736.0
31	19125.0
32	23291.5
33	27559.0
34	31279.5
35	34873.0
36	38892.5
37	43169.0
38	46446.5
39	48506.5
40	50260.0
41	51667.0
42	53420.5
43	57693.0
44	66319.0
45	84835.0
46	155367.0
47	730160.5
48	2039397.5
49	2142072.5
50	959231.0
51	321926.5
52	139694.0
53	87671.5
54	66644.0
55	53400.0
56	44469.0
57	37667.5
58	31674.5
59	27427.5
60	22916.0
61	17925.5
62	13841.5
63	10536.5
64	7787.5
65	5634.0
66	4149.5
67	3169.0
68	2442.5
69	1896.0
70	1426.5
71	1077.5
72	843.5
73	689.0
74	584.0
75	453.5
76	310.5
77	228.5
78	172.5
79	116.0
80	74.0
81	48.0
82	36.5
83	25.0
84	28.5
85	29.0
86	21.0
87	19.5
88	23.5
89	21.5
90	11.0
91	9.0
92	12.0
93	12.0
94	8.5
95	3.5
96	4.0
97	7.5
98	11.0
99	10.0
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06129636538728247
2	0.01033977355375022
3	0.012254064123525135
4	0.010274661629608215
5	0.012774959516661166
6	0.012957272904258774
7	0.0034639543643546072
8	0.006146565639005168
9	0.00817805767223569
10-11	0.007813430897040468
12-13	0.006726061763869003
14-15	0.01117971737518207
16-17	0.010671844366874439
18-19	0.008614307563987116
20-21	0.007605072739786055
22-23	0.002806323930520368
24-25	0.004329942955443259
26-27	0.005560558321727133
28-29	0.006713039379040602
30-31	0.009838411737856788
32-33	0.011329474800708679
34-35	0.012918205749773572
36-37	0.018836879654281727
38-39	0.019260107161204753
40-41	0.019338241470175155
42-43	0.024521150631878666
44-45	0.02855157873626871
46-47	0.030823984888824645
48-49	0.03221086887304933
50-51	0.032803387382741564
52-53	0.03092816396745185
54-55	0.03693799456575881
56-57	0.03969222895696558
58-59	0.03693799456575881
60-61	0.037595624999593055
62-63	0.03313545819586578
64-65	0.035446931502906925
66-67	0.03776491600236226
68-69	0.040265213889415216
70-71	0.039998255000433
72-73	0.036104561936741164
74-75	0.03886530752036212
76-77	0.0385137031299953
78-79	0.03799280773685927
80-81	0.04261575435094155
82-83	0.03891088586726153
84-85	0.03523857334565251
86-87	0.0334610178165758
88-89	0.03136441385920328
90-91	0.03368239835865862
92-93	0.0351604390366821
94-95	0.038533236707237906
96-97	0.03409260348075324
98-99	0.03133836908954648
100	0.029052940552162135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7679085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.99225455855619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49861732223206	39.743151142262704
2	13.342722994043541	13.340656088450512
3	3.985034895843557	5.976626368132215
4	1.5531878242055084	3.1058944433972715
5	0.6971523728205672	1.742610944407363
6	0.36579490679824567	1.097214725812874
7	0.20716250825967733	0.724956459353475
8	0.11593463830543782	0.46366671602556697
9	0.07204111712395542	0.32413480793491867
>10	0.16051032920220737	1.05703496869903
>50	6.945276953792816E-4	0.02187838909658662
>100	3.496694187102862E-4	0.05274744868202176
>500	9.107622607358465E-5	0.032360987071404196
>1k	1.821465355338338E-4	0.22133067005194756
>5k	0.0	0.0
>10k+	5.236712895357201E-4	32.09573584062208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	866667	11.286071192075617	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	478223	6.227603939792306	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	203418	2.64898747702363	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	185026	2.409479775259683	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	147142	1.9161397484205474	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	133657	1.740532889009563	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	127753	1.663648728982685	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	127643	1.6622162666515605	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	99880	1.30067579666067	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	67415	0.8779040732066385	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	66167	0.8616521369407943	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	63844	0.8314011369844193	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	35447	0.46160447501232243	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	32756	0.42656123743909596	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	27156	0.35363588240005156	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	26099	0.33987122163643196	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	21977	0.28619295137376394	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	21576	0.28097097505757523	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	20753	0.2702535523438014	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	19511	0.2540797503869276	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	17047	0.2219925941697481	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	16942	0.220625243762766	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	13671	0.17802902298906706	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.302238482840078E-5
2	0.0	0.0	0.0	2.604476965680156E-5	1.302238482840078E-5
3	0.0	0.0	0.0	2.604476965680156E-5	1.302238482840078E-5
4	0.0	0.0	0.0	2.604476965680156E-5	1.302238482840078E-5
5	0.0	0.0	0.0	2.604476965680156E-5	1.302238482840078E-5
6	0.0	0.0	0.0	2.604476965680156E-5	1.302238482840078E-5
7	0.0	0.0	0.0	2.604476965680156E-5	1.302238482840078E-5
8	0.0	0.0	0.0	3.906715448520234E-5	1.302238482840078E-5
9	0.0	0.0	0.0	7.813430897040468E-5	1.302238482840078E-5
10-11	0.0	0.0	0.0	7.618095124614456E-4	2.604476965680156E-5
12-13	0.0	0.0	0.0	0.0013933951766388834	7.813430897040468E-5
14-15	0.0	0.0	0.0	0.0024091411932541445	1.0417907862720623E-4
16-17	0.0	0.0	0.0	0.004883394310650293	1.1720146345560701E-4
18-19	0.0	0.0	0.0	0.008106434555679484	1.302238482840078E-4
20-21	0.0	0.0	0.0	0.014435313582282264	1.302238482840078E-4
22-23	0.0	0.0	0.0	0.023160311417310786	1.302238482840078E-4
24-25	0.0	0.0	0.0	0.030023108221877995	1.302238482840078E-4
26-27	0.0	0.0	0.0	0.03531019646220872	1.302238482840078E-4
28-29	0.0	0.0	0.0	0.04665920484015999	1.302238482840078E-4
30-31	0.0	0.0	0.0	0.05919325023749575	1.3673504069820817E-4
32-33	0.0	0.0	0.0	0.10759094345224723	1.4324623311240858E-4
34-35	0.0	0.0	0.0	0.16467456734754204	1.6929100276921014E-4
36-37	0.0	0.0	0.0	0.20460119923141884	1.6929100276921014E-4
38-39	6.51119241420039E-6	0.0	0.0	0.2554731455635665	1.6929100276921014E-4
40-41	1.302238482840078E-5	0.0	0.0	0.30478240571630605	1.6929100276921014E-4
42-43	1.302238482840078E-5	0.0	0.0	0.3648742005069614	1.6929100276921014E-4
44-45	1.302238482840078E-5	0.0	0.0	0.4400719616985617	1.953357724260117E-4
46-47	1.302238482840078E-5	0.0	0.0	0.5368152585887511	2.2138054208281326E-4
48-49	1.302238482840078E-5	0.0	0.0	0.5971675010759745	2.3440292691121403E-4
50-51	1.302238482840078E-5	0.0	0.0	0.6688101512094214	2.3440292691121403E-4
52-53	1.302238482840078E-5	0.0	0.0	0.7400295738359453	2.604476965680156E-4
54-55	1.302238482840078E-5	0.0	0.0	0.8406665637898265	2.604476965680156E-4
56-57	1.302238482840078E-5	0.0	0.0	1.0181160906540299	2.604476965680156E-4
58-59	1.302238482840078E-5	0.0	0.0	1.1431830745459908	2.604476965680156E-4
60-61	1.302238482840078E-5	0.0	0.0	1.250481014339599	2.604476965680156E-4
62-63	1.302238482840078E-5	0.0	0.0	1.3524593099308055	2.9951485105321794E-4
64-65	1.302238482840078E-5	0.0	0.0	1.4470799580939655	2.9951485105321794E-4
66-67	1.302238482840078E-5	0.0	0.0	1.5790943842918785	2.9951485105321794E-4
68-69	1.302238482840078E-5	0.0	0.0	1.7335073123946407	2.9951485105321794E-4
70-71	1.302238482840078E-5	0.0	0.0	1.8669737345009203	3.125372358816187E-4
72-73	1.302238482840078E-5	0.0	0.0	1.9698049962983872	3.320708131242199E-4
74-75	1.302238482840078E-5	0.0	0.0	2.1100300361306066	3.385820055384203E-4
76-77	1.302238482840078E-5	0.0	0.0	2.222784355167315	3.385820055384203E-4
78-79	1.302238482840078E-5	0.0	0.0	2.47599160577074	3.385820055384203E-4
80-81	1.302238482840078E-5	0.0	0.0	2.7306899194370162	3.385820055384203E-4
82-83	1.302238482840078E-5	0.0	0.0	2.8782921402745245	3.385820055384203E-4
84-85	1.302238482840078E-5	0.0	0.0	3.0481431055913566	3.385820055384203E-4
86-87	1.302238482840078E-5	0.0	0.0	3.2049716860797868	3.5811558278102146E-4
88	1.302238482840078E-5	0.0	0.0	3.3459064458851544	3.776491600236226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGC	19260	0.0	38.821037	2
GTACGCA	19250	0.0	38.719387	3
GAGTACG	19535	0.0	38.491817	1
TACGCAG	19725	0.0	37.739334	4
CGCAGTG	19965	0.0	37.355556	6
ACGCAGT	20520	0.0	36.345917	5
GCTTATG	3540	0.0	26.03671	1
GTAGGCA	7460	0.0	26.017605	3
AGTAGGC	7460	0.0	25.95444	2
TAGGCAG	7635	0.0	25.2366	4
AGTAAGG	2535	0.0	24.475002	94
GAGTAGG	8055	0.0	24.286285	1
TGCCTAC	6640	0.0	23.922241	2
CTGCCTA	6735	0.0	23.600021	1
GCCTACT	6655	0.0	23.515392	3
AGCTTAC	1825	0.0	23.448326	1
AGGCAGT	8410	0.0	23.413918	5
CTTAGGT	735	0.0	23.018044	2
GGCAGTG	8625	0.0	22.938793	6
GCTTAGG	865	0.0	22.833185	1
>>END_MODULE