FastQCFastQC Report
Mon 16 Jan 2017
ERR1146559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7679085
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90417311.774488757449618No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2148702.7981198280784754No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2064872.688953176061992No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1576422.0528747891187558No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1447931.885550166458634No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1446101.8831670700350367No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1318231.7166498352342758No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1103741.4373327030499077No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG761930.9922145672303406No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT731100.952066554804381No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT710140.9247716362040529No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA408940.5325374051726215No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG380310.49525431740891No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT325420.42377444708581813No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA256900.33454506624161606No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC228160.2971187322447922No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT219140.28537254112957466No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA212550.27679078952765857No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG196630.2560591528808445No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT176670.2300664727633566No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC134030.17453902385505565No Hit
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG91320.1189204182529559No Hit
GTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA85590.11145859174628227No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG84570.1101303084937854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG219700.036.760151
GTACGCA220200.036.6002853
TACGCAG221750.036.3020444
AGTACGC222500.036.1899032
CGCAGTG226150.035.6841356
ACGCAGT231750.034.7559055
TGCGCGA3600.027.41492894
AGTAGGC81600.025.8954562
GTAGGCA82200.025.7985743
TAGGCAG82700.025.5288814
GAGTAGG86050.025.26861
GCTTATG37250.024.7639451
GGCAGTG91150.023.306836
AGGCAGT91800.023.1006815
TTATGTA41600.022.154083
TACCTGG33250.022.0800652
CACGCGA550.003062372621.36228290-91
GTACCTG35450.020.8436361
ATAACGC2951.5279511E-1020.7210753
ACCTGGG33200.020.3946083
Produced by FastQC (version 0.11.5)