FastQCFastQC Report
Mon 16 Jan 2017
ERR1146558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945171
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70400.035.46091
GTACTAG21400.034.271941
GGTATCA47550.034.2100261
CAACGCA71650.031.8753665
ATCAACG73650.031.0097793
TATCAAC88550.027.8085172
AACGCAG91150.026.8602896
ACGCAGA87250.026.228367
GCATCGT23550.026.1396038
GTACATG140750.025.419331
CGCAGAG89800.025.2748998
TACATGG138250.025.2216722
GTGATCG15450.024.3321298
ACATGGG139950.024.2437293
TAGGCAT33800.024.1917725
TGATCGC16200.023.7857849
TCAACGC98400.023.7353944
CTAGGCA38650.022.4935174
TCTAACG10700.022.3986342
CTAACGC11100.022.0148373
Produced by FastQC (version 0.11.5)