FastQCFastQC Report
Mon 16 Jan 2017
ERR1146558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945171
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138600.35131557035170335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125390.3178315971601738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.18103144325049533No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT41870.10612974697421226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88400.055.1451841
GTATCAA154550.041.8633351
ATCAACG187100.033.745143
CAACGCA190600.033.100815
TATCAAC200350.032.0799672
GTACTAG21950.030.8693921
AACGCAG210200.030.675976
TCAACGC210450.030.1350574
ACGCAGA220800.028.5849447
CGCAGAG222900.028.2734688
GTGGTAT22850.026.152771
GCAGAGT244350.025.6376489
GTACATG144550.023.8608531
GAGTACT165300.023.71120312-13
TACATGG141400.023.692082
ACATGGG141550.023.1489853
CAGAGTA241650.022.53043710-11
AGAGTAC229050.022.26177610-11
AGTACTT170750.021.7020812-13
GCTATTG28950.021.5905388
Produced by FastQC (version 0.11.5)