FastQCFastQC Report
Mon 16 Jan 2017
ERR1146557_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146557_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617618
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67200.036.2351881
GGTATCA39950.035.6529851
CAACGCA66000.033.5355835
ATCAACG66400.033.3342863
TCAACGC76650.029.2442074
TATCAAC81600.028.9102522
AACGCAG78850.028.9034796
ACGCAGA85150.025.8265617
CGCAGAG89150.024.8272428
GTACTAG18400.024.014861
GTACATG141600.023.703091
TACATGG140400.022.9612372
ACATGGG140750.021.9025023
CGACGAG8950.019.4262424-25
GTGATCG12700.019.241048
GTATAAG25050.019.1409051
GCAGAGT109800.019.1308429
CATGGGG109400.019.0721324
TAGTACT25300.018.9458144
TGATCGC12900.018.5784459
Produced by FastQC (version 0.11.5)