##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146557_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4617618
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406051561649317	31.0	31.0	34.0	28.0	34.0
2	31.465694217234947	31.0	31.0	34.0	28.0	34.0
3	31.697833601653493	33.0	31.0	34.0	29.0	34.0
4	35.25599259185147	37.0	35.0	37.0	32.0	37.0
5	35.23542137959441	37.0	35.0	37.0	32.0	37.0
6	35.21414439219528	37.0	35.0	37.0	32.0	37.0
7	35.24871849512021	37.0	35.0	37.0	32.0	37.0
8	35.17810438195624	37.0	35.0	37.0	32.0	37.0
9	36.84173116962035	39.0	37.0	39.0	33.0	39.0
10-11	36.81546394699605	39.0	37.0	39.0	33.0	39.0
12-13	36.768846730067324	39.0	37.0	39.0	33.0	39.0
14-15	38.17499769361606	40.0	38.0	41.0	33.0	41.0
16-17	38.179500123223704	40.0	38.0	41.0	33.0	41.0
18-19	38.20238486596336	40.0	38.5	41.0	33.0	41.0
20-21	38.292765772309444	40.0	39.0	41.0	33.0	41.0
22-23	38.30046584624367	40.0	38.5	41.0	33.0	41.0
24-25	38.346762984725025	40.0	38.0	41.0	33.0	41.0
26-27	38.36579065223672	40.0	38.0	41.0	33.0	41.0
28-29	38.32944485663388	40.0	38.0	41.0	33.0	41.0
30-31	38.31175694914564	40.0	38.0	41.0	33.0	41.0
32-33	38.324135084366006	40.0	38.0	41.0	33.0	41.0
34-35	38.26440017342274	40.0	38.0	41.0	33.0	41.0
36-37	38.176399606896894	40.0	38.0	41.0	33.0	41.0
38-39	38.14145150161836	40.0	38.0	41.0	33.0	41.0
40-41	38.08397305710433	40.0	38.0	41.0	33.0	41.0
42-43	37.96809632152335	40.0	38.0	41.0	32.0	41.0
44-45	37.83534248610431	40.0	38.0	41.0	32.0	41.0
46-47	37.73568385258373	40.0	37.0	41.0	32.0	41.0
48-49	37.62573647278748	40.0	37.0	41.0	31.5	41.0
50-51	37.5061922618978	40.0	37.0	41.0	31.0	41.0
52-53	37.388757905049744	40.0	36.5	41.0	31.0	41.0
54-55	37.213867084717705	40.0	36.0	41.0	31.0	41.0
56-57	37.09124704555465	39.5	35.5	41.0	31.0	41.0
58-59	36.87540383375152	39.0	35.0	41.0	30.5	41.0
60-61	36.697152406283934	39.0	35.0	41.0	30.0	41.0
62-63	36.46213978722363	38.5	35.0	40.5	30.0	41.0
64-65	36.214015537881224	38.0	35.0	40.0	30.0	41.0
66-67	35.94880130838021	37.0	35.0	40.0	30.0	41.0
68-69	35.65247081937051	37.0	35.0	40.0	30.0	41.0
70-71	35.38047755357849	36.0	35.0	39.0	29.0	41.0
72-73	35.05730422048771	36.0	34.0	39.0	29.0	41.0
74-75	34.734406462379525	35.0	34.0	38.5	29.0	40.0
76-77	34.419843737615366	35.0	34.0	37.0	29.0	39.5
78-79	34.13643257194511	35.0	34.0	37.0	29.0	39.0
80-81	33.850977062199604	35.0	34.0	36.5	29.0	39.0
82-83	33.59773383159889	35.0	34.0	36.0	28.0	37.5
84-85	33.37308423953649	35.0	34.0	36.0	28.0	37.0
86-87	33.17562583132688	35.0	34.0	35.0	28.0	37.0
88-89	33.02296963499363	35.0	33.5	35.0	28.0	36.0
90-91	32.86443670741062	35.0	33.0	35.0	27.5	36.0
92-93	32.77530742040594	35.0	33.0	35.0	27.0	36.0
94-95	32.6826403786541	35.0	33.0	35.0	27.0	36.0
96-97	32.6112449102546	35.0	33.0	35.0	27.0	35.0
98-99	32.542344126343934	35.0	33.0	35.0	27.0	35.0
100	31.634965040416944	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2688169678949457
1101	2	0.2927263490803149
1101	3	0.3582658495119837
1101	4	0.3621962675880397
1101	5	0.32268372557766156
1101	6	0.29815479865546735
1101	7	0.33806627095280817
1101	8	0.4077311161973185
1101	9	0.5209220656528188
1101	10-11	0.5565847878980392
1101	12-13	0.5287538320595502
1101	14-15	0.6248879618103871
1101	16-17	0.6452506562054765
1101	18-19	0.5449659854340183
1101	20-21	0.5073170710296893
1101	22-23	0.5150961551851267
1101	24-25	0.5595565928459436
1101	26-27	0.5082384655596499
1101	28-29	0.5620377100373801
1101	30-31	0.5601818622645851
1101	32-33	0.5042762828289185
1101	34-35	0.5168032521578496
1101	36-37	0.5475909080814034
1101	38-39	0.5452768683708058
1101	40-41	0.5327889335855218
1101	42-43	0.4731357047221749
1101	44-45	0.47399822575111017
1101	46-47	0.42077901654896266
1101	48-49	0.4463781160349498
1101	50-51	0.4964497367726821
1101	52-53	0.4712920961081224
1101	54-55	0.5121528716458741
1101	56-57	0.4804881890693551
1101	58-59	0.4379216172307636
1101	60-61	0.4982266907303128
1101	62-63	0.4561458151466127
1101	64-65	0.4508881132482969
1101	66-67	0.462654517447163
1101	68-69	0.4406482620559018
1101	70-71	0.38794543218545385
1101	72-73	0.3953348901688116
1101	74-75	0.3792787295340432
1101	76-77	0.3571146690181948
1101	78-79	0.3364140301182559
1101	80-81	0.40377912170967534
1101	82-83	0.367597397477482
1101	84-85	0.3584876054667987
1101	86-87	0.3784521927532296
1101	88-89	0.3572855920521434
1101	90-91	0.40844319617505676
1101	92-93	0.40216964839997615
1101	94-95	0.41274153014195036
1101	96-97	0.3679570572110009
1101	98-99	0.4021431980134338
1101	100	0.2760009235896064
1102	1	0.24121071737977928
1102	2	0.1944928698214703
1102	3	0.28028930868613244
1102	4	0.26427456566580076
1102	5	0.2920528082635414
1102	6	0.23328076941481868
1102	7	0.29708591317597666
1102	8	0.3331840703050091
1102	9	0.4054227417420151
1102	10-11	0.4304818975288853
1102	12-13	0.4095693256838615
1102	14-15	0.4901745961911814
1102	16-17	0.4893325418000032
1102	18-19	0.4124158626471228
1102	20-21	0.4382352746657716
1102	22-23	0.447852955589525
1102	24-25	0.4285334428558585
1102	26-27	0.4614633881604604
1102	28-29	0.5102545645177514
1102	30-31	0.48092456936298333
1102	32-33	0.43302814844582826
1102	34-35	0.4228342014611428
1102	36-37	0.42814396610930316
1102	38-39	0.365203636807216
1102	40-41	0.39637460941762725
1102	42-43	0.37752313768349666
1102	44-45	0.3625818158176486
1102	46-47	0.34039918860119656
1102	48-49	0.37324766188346814
1102	50-51	0.427149660023197
1102	52-53	0.4023866835687855
1102	54-55	0.3916210109076488
1102	56-57	0.32344127953258095
1102	58-59	0.37927659579143835
1102	60-61	0.2969440954847258
1102	62-63	0.2942285872472752
1102	64-65	0.2942734702594194
1102	66-67	0.2590702324972298
1102	68-69	0.2993030263627219
1102	70-71	0.34323816269014884
1102	72-73	0.31592247542448604
1102	74-75	0.22933481376238518
1102	76-77	0.22323078559486476
1102	78-79	0.243142762810443
1102	80-81	0.28800970788898894
1102	82-83	0.28125522183660934
1102	84-85	0.2979074648326616
1102	86-87	0.27283641397019665
1102	88-89	0.2517237141359132
1102	90-91	0.2607807398548303
1102	92-93	0.22576733634557655
1102	94-95	0.19849859277281467
1102	96-97	0.24871947256321647
1102	98-99	0.23697469938652915
1102	100	0.2115273446066297
1103	1	0.21569021400744148
1103	2	0.22609047363123835
1103	3	0.26179231469815534
1103	4	0.26686350698930283
1103	5	0.2515843444542014
1103	6	0.2475515690786665
1103	7	0.23425509023978464
1103	8	0.31528062901382725
1103	9	0.4057123532230378
1103	10-11	0.44311167320928035
1103	12-13	0.34525542907343265
1103	14-15	0.4840051605457063
1103	16-17	0.42783907098741025
1103	18-19	0.38268187854988867
1103	20-21	0.3835491820504302
1103	22-23	0.3613269465822526
1103	24-25	0.37924324956605204
1103	26-27	0.3467567706243173
1103	28-29	0.4104001783260074
1103	30-31	0.36381949900721366
1103	32-33	0.31633077520471886
1103	34-35	0.337753017588021
1103	36-37	0.34700846930924456
1103	38-39	0.34757779372319675
1103	40-41	0.3082943090319006
1103	42-43	0.31554197104915005
1103	44-45	0.26191252023723877
1103	46-47	0.2462371548562885
1103	48-49	0.2662149943979628
1103	50-51	0.24998673201664445
1103	52-53	0.3002894784057659
1103	54-55	0.27967690764736375
1103	56-57	0.24302765658777048
1103	58-59	0.29035838302181105
1103	60-61	0.23582637617513313
1103	62-63	0.2797322828104498
1103	64-65	0.25687033064686204
1103	66-67	0.26365924924039064
1103	68-69	0.2432583025064048
1103	70-71	0.26402678294140713
1103	72-73	0.2653421211675777
1103	74-75	0.2227205637570009
1103	76-77	0.2643731240351883
1103	78-79	0.23129492735458967
1103	80-81	0.24344843480518819
1103	82-83	0.2036580382220663
1103	84-85	0.23930520655265752
1103	86-87	0.1998484487925225
1103	88-89	0.19293958674133904
1103	90-91	0.24671551262618863
1103	92-93	0.205262617229927
1103	94-95	0.16130533529010194
1103	96-97	0.20575908943136056
1103	98-99	0.09893555658479869
1103	100	0.18266284140668176
1104	1	0.16856357123563726
1104	2	0.1459729905567677
1104	3	0.20802688259939117
1104	4	0.2361745384508751
1104	5	0.21453635560514073
1104	6	0.22155794104202897
1104	7	0.20761530529354388
1104	8	0.1891147588425781
1104	9	0.2819538904591994
1104	10-11	0.27033738009896524
1104	12-13	0.2608249232988413
1104	14-15	0.3554194377261055
1104	16-17	0.3410215680005564
1104	18-19	0.25548703585774035
1104	20-21	0.28608900682143457
1104	22-23	0.35333212380248824
1104	24-25	0.28551515319011145
1104	26-27	0.3032661294455039
1104	28-29	0.3030161679715704
1104	30-31	0.25200299163962825
1104	32-33	0.2915000304815081
1104	34-35	0.3069510462618581
1104	36-37	0.267638696310442
1104	38-39	0.2421516686734151
1104	40-41	0.25606760652294724
1104	42-43	0.24918095949997365
1104	44-45	0.19009053696364475
1104	46-47	0.17129987887062015
1104	48-49	0.21704683430237992
1104	50-51	0.2398463496988441
1104	52-53	0.182998060643925
1104	54-55	0.2996047252499139
1104	56-57	0.24705842122337884
1104	58-59	0.2798501193818197
1104	60-61	0.2530983793611199
1104	62-63	0.22453116771685444
1104	64-65	0.20003391089377232
1104	66-67	0.21123242725711577
1104	68-69	0.1898393459154093
1104	70-71	0.2155998613962069
1104	72-73	0.18307808293580052
1104	74-75	0.18619328378886024
1104	76-77	0.21083021284442793
1104	78-79	0.22771020015427013
1104	80-81	0.23612167255907934
1104	82-83	0.19187538190984554
1104	84-85	0.15758577206440805
1104	86-87	0.18227875544326366
1104	88-89	0.1796346285573449
1104	90-91	0.1005834935102925
1104	92-93	0.127377810380068
1104	94-95	0.0721356817656087
1104	96-97	0.07495960715338157
1104	98-99	0.09762532161864357
1104	100	0.04903878724499222
1105	1	0.1589932532893208
1105	2	0.1827718420609834
1105	3	0.22231332057076614
1105	4	0.19905194052292785
1105	5	0.1681124615760936
1105	6	0.17010600850917967
1105	7	0.23018030554451485
1105	8	0.25064444907589944
1105	9	0.30840714753598064
1105	10-11	0.34951930225853545
1105	12-13	0.3636114384190918
1105	14-15	0.39919739257449294
1105	16-17	0.39615567458869805
1105	18-19	0.2794198601014415
1105	20-21	0.3305351708492452
1105	22-23	0.3510826066800874
1105	24-25	0.3088319483142996
1105	26-27	0.32771363195981706
1105	28-29	0.3345138344504832
1105	30-31	0.3141226500095371
1105	32-33	0.2800263661330291
1105	34-35	0.2576875275481427
1105	36-37	0.23466877600615987
1105	38-39	0.22771760214691739
1105	40-41	0.2648368890941768
1105	42-43	0.2921824643677837
1105	44-45	0.19448816226550036
1105	46-47	0.22690688250126811
1105	48-49	0.14328580573127425
1105	50-51	0.1139315081142982
1105	52-53	0.07389078699221585
1105	54-55	0.1762995256945885
1105	56-57	0.13765956200426643
1105	58-59	0.16115384474298367
1105	60-61	0.1062943876969129
1105	62-63	0.0954855457760928
1105	64-65	0.10540255470949944
1105	66-67	0.10749404141607499
1105	68-69	0.13560036379721652
1105	70-71	0.06541096284046688
1105	72-73	0.09884998531551759
1105	74-75	0.15641128513106395
1105	76-77	0.165915218356389
1105	78-79	0.10119995283170624
1105	80-81	0.09067371179904171
1105	82-83	0.08587023663758231
1105	84-85	0.08790051623687845
1105	86-87	0.08762080381096382
1105	88-89	0.08927629759424605
1105	90-91	0.10347678546208527
1105	92-93	0.15426618014068083
1105	94-95	0.12741315280690202
1105	96-97	0.16196643529357146
1105	98-99	0.08899582490816016
1105	100	0.027725016263751456
1106	1	0.13375859048824879
1106	2	0.15608661327946294
1106	3	0.16376688463083866
1106	4	0.174340646468643
1106	5	0.11907583961669843
1106	6	0.11627270691734992
1106	7	0.11857578896345444
1106	8	0.2184785685510633
1106	9	0.1910082733137557
1106	10-11	0.22067735950744094
1106	12-13	0.1801479424037069
1106	14-15	0.2270403621284416
1106	16-17	0.21463250597667383
1106	18-19	0.20065097160849632
1106	20-21	0.21593493976510558
1106	22-23	0.2308009133349742
1106	24-25	0.21840012461129987
1106	26-27	0.25304890274485814
1106	28-29	0.2603806956131507
1106	30-31	0.23146058280097748
1106	32-33	0.2630114758585691
1106	34-35	0.250374859940905
1106	36-37	0.25646283271311177
1106	38-39	0.21376503284785997
1106	40-41	0.23172465380957163
1106	42-43	0.172092143558622
1106	44-45	0.11287302401314747
1106	46-47	0.11529347980139448
1106	48-49	0.06821670742905894
1106	50-51	0.09769525133047097
1106	52-53	0.10225765463082581
1106	54-55	0.17600752264965536
1106	56-57	0.08336604054800745
1106	58-59	0.147765493307233
1106	60-61	0.07474209987045555
1106	62-63	0.12862315549121917
1106	64-65	0.13221999407244311
1106	66-67	0.08421592402988409
1106	68-69	0.06124494810198655
1106	70-71	0.1326967108010777
1106	72-73	0.15023264400397807
1106	74-75	0.07291533056761779
1106	76-77	0.03241743363540195
1106	78-79	0.04049939329905072
1106	80-81	0.06506049357904686
1106	82-83	0.07609337732984756
1106	84-85	0.1255335222329279
1106	86-87	0.08329725087711637
1106	88-89	0.03555774969643011
1106	90-91	0.09013964465021473
1106	92-93	0.08373180679732428
1106	94-95	0.025628507051628446
1106	96-97	0.060744070250237314
1106	98-99	0.004635788205895608
1106	100	0.020979607396981237
1107	1	0.13575368011161615
1107	2	0.10546700975720569
1107	3	0.1724735764005878
1107	4	0.1711857763439113
1107	5	0.21130650601865142
1107	6	0.13659142356194565
1107	7	0.09140413336978526
1107	8	0.14140187900801493
1107	9	0.18402069813297572
1107	10-11	0.24091700510540903
1107	12-13	0.22999108763880116
1107	14-15	0.2508574555004657
1107	16-17	0.18058643082535042
1107	18-19	0.1683951985333465
1107	20-21	0.13072971431082436
1107	22-23	0.17256041217535767
1107	24-25	0.19416087372220403
1107	26-27	0.19354192903404055
1107	28-29	0.17840117027427738
1107	30-31	0.20463052705588325
1107	32-33	0.18080985136869288
1107	34-35	0.19332471198433154
1107	36-37	0.22170400856205674
1107	38-39	0.1675149664615816
1107	40-41	0.14663314415476947
1107	42-43	0.1195797580056066
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2216	54-55	-0.4962657594323048
2216	56-57	-0.5027615376243659
2216	58-59	-0.4814810420264948
2216	60-61	-0.5070868058240663
2216	62-63	-0.5018977303169763
2216	64-65	-0.5001747082191628
2216	66-67	-0.5044254373976571
2216	68-69	-0.5442770235404666
2216	70-71	-0.5235233667702346
2216	72-73	-0.45978314248715435
2216	74-75	-0.4688307216981116
2216	76-77	-0.4475059782849726
2216	78-79	-0.39860724383293444
2216	80-81	-0.40309300508153
2216	82-83	-0.36957009134118124
2216	84-85	-0.33018857635808274
2216	86-87	-0.3343536176705868
2216	88-89	-0.43067915745088925
2216	90-91	-0.4316352993566497
2216	92-93	-0.37000159062587556
2216	94-95	-0.33720931137568044
2216	96-97	-0.32611492416805987
2216	98-99	-0.33403334321902634
2216	100	-0.37831603671477154
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	130.0
1	59.0
2	67.0
3	55.0
4	61.0
5	84.0
6	153.0
7	507.0
8	1540.0
9	2365.0
10	2696.0
11	4051.0
12	5884.0
13	7672.0
14	9150.0
15	10002.0
16	10968.0
17	12090.0
18	13274.0
19	14653.0
20	16558.0
21	18284.0
22	20771.0
23	23987.0
24	28489.0
25	35786.0
26	41030.0
27	40443.0
28	43486.0
29	54362.0
30	63090.0
31	70057.0
32	88225.0
33	117572.0
34	172337.0
35	263120.0
36	493949.0
37	1084062.0
38	1403943.0
39	441668.0
40	938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.584647241583596	19.57243321462979	13.239930676090141	24.60298886769647
2	14.974666234077327	20.691343029629916	40.216687408141546	24.117303328151213
3	17.857254245569898	25.834234341943162	30.46435731229132	25.84415410019562
4	12.291595510056718	17.580752509712134	39.717202966410326	30.410449013820823
5	13.276937844972403	38.246268818350124	35.48557992616908	12.991213410508387
6	29.969900557263124	38.328219294269104	18.672765538184837	13.029114610282932
7	26.58720198307259	32.612868358310514	23.25529872239864	17.544630936218255
8	25.211820266048246	35.42287602564064	21.10704866289337	18.258255045417748
9	26.16066671575783	15.704703655414685	20.70951170174519	37.4251179270823
10-11	24.25583796846364	26.38971609655394	29.278206145011104	20.07623978997131
12-13	24.899901468934775	24.22239887571279	28.446716611163758	22.430983044188675
14-15	22.484409899044046	25.550438721170426	25.87526294843368	26.08988843135185
16-17	22.374576333225114	28.22720429634767	26.691109695609676	22.707109674817545
18-19	22.179280373107144	28.14752840774959	27.730319027052175	21.942872192091095
20-21	23.488588721397083	27.340515204425813	27.033786602224602	22.1371094719525
22-23	23.278460880096052	27.260835743008766	26.726617629988205	22.73408574690698
24-25	22.902909055312854	27.355307395173607	27.094329676764893	22.647453872748645
26-27	22.78952510885762	27.507531590665668	26.80454703306601	22.898396267410696
28-29	22.712939356215852	27.691656465953006	26.928248916471965	22.667155261359177
30-31	23.04372511803485	27.440873200698825	26.83304475496865	22.682356926297675
32-33	22.93662509941314	27.649186848183362	26.57580652427413	22.83838152812937
34-35	23.12661542081919	27.610314825056705	26.829790485192355	22.433279268931756
36-37	23.057292032751054	27.299450376583394	26.777530692063706	22.86572689860185
38-39	22.863194498746182	27.540037858267812	26.950687429291918	22.646080213694088
40-41	23.030960586883623	27.188345330114455	27.079880984884415	22.700813098117507
42-43	22.839916142775632	27.218733225630782	26.970705425721846	22.97064520587174
44-45	22.920963304774467	27.497411592016864	26.89180156769953	22.689823535509145
46-47	22.952949939846885	27.299116913028893	26.707366647411746	23.040566499712476
48-49	23.041404073239587	27.25247779191563	26.8583100874762	22.84780804736858
50-51	22.898715650707945	27.285089321265833	26.776882492633288	23.039312535392938
52-53	22.890648711050375	27.267795940446128	26.759967184583257	23.08158816392024
54-55	22.858975987982237	27.3151041268919	26.73790622259095	23.088013662534912
56-57	23.064902257724164	27.299602877997103	26.68833180594101	22.947163058337722
58-59	22.895323239020644	27.28469300289969	26.96157692863055	22.858406829449113
60-61	22.923690101622753	27.379518640009515	26.813592538839988	22.883198719527744
62-63	22.95921506371942	27.200218889961004	26.7986173364142	23.041948709905377
64-65	22.697660744979217	27.261025660356758	26.785789336629755	23.25552425803427
66-67	23.001733831420804	27.150136195566997	26.640245037197325	23.207884935814874
68-69	22.735804908485857	27.157699407237935	26.854396318635608	23.2520993656406
70-71	22.854825296453477	26.872436529269024	26.896614926845725	23.376123247431778
72-73	22.92197375731348	26.9295874792144	26.978202298090082	23.170236465382036
74-75	22.796941257471968	27.17758077609439	26.8903687004049	23.13510926602875
76-77	22.872648747253294	27.080105486477574	26.740117954636915	23.30712781163222
78-79	22.80890086327827	27.151788312130332	26.718535145870348	23.32077567872105
80-81	22.777889116260123	27.190717042695837	26.84373818533199	23.187655655712046
82-83	23.040237604139485	26.92944767631744	27.142132758864797	22.888181960678285
84-85	23.1241783211424	27.055346695536254	26.712403923759247	23.108071059562096
86-87	22.627537745834818	27.25579212851976	26.909172128459108	23.207497997186312
88-89	23.063520139858472	26.92641724227946	26.750987577162082	23.259075040699987
90-91	22.935694055563612	26.916358218830048	26.87900977222972	23.268937953376618
92-93	23.014514537067768	26.90404998002567	26.966041901903065	23.1153935810035
94-95	22.657055832372677	26.939381419713275	27.081199792970306	23.322362954943742
96-97	22.752315102662614	26.877872891515892	27.042138702953256	23.327673302868245
98-99	22.660099375892187	27.037400217650813	26.886688888121046	23.41581151833595
100	22.941405968215125	26.794341085489627	26.674329500255727	23.58992344603952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	939.0
1	714.0
2	512.5
3	531.0
4	506.0
5	478.0
6	436.0
7	412.0
8	412.5
9	433.0
10	413.5
11	369.0
12	399.0
13	425.5
14	460.0
15	553.5
16	654.0
17	800.5
18	1042.5
19	1359.0
20	1965.0
21	2956.5
22	4425.5
23	6662.5
24	9528.5
25	13283.5
26	18956.5
27	25743.0
28	32181.5
29	40035.0
30	48935.0
31	57860.0
32	69120.5
33	79828.5
34	89350.0
35	100619.0
36	114277.0
37	128623.5
38	135836.5
39	137465.5
40	139067.0
41	141765.0
42	145457.5
43	153135.0
44	162199.0
45	172981.5
46	182118.5
47	187329.0
48	190657.0
49	187396.5
50	181862.0
51	181402.0
52	179442.5
53	173739.5
54	166278.5
55	151951.0
56	139029.5
57	125016.5
58	109314.5
59	95539.0
60	77939.5
61	60587.0
62	47183.0
63	36122.0
64	26958.5
65	19479.0
66	14162.0
67	10414.0
68	7817.0
69	5673.5
70	4101.0
71	3190.0
72	2445.0
73	1863.5
74	1422.5
75	1062.5
76	767.0
77	486.0
78	313.5
79	217.5
80	156.0
81	114.0
82	75.5
83	69.5
84	56.5
85	39.0
86	38.5
87	35.0
88	26.5
89	17.5
90	11.5
91	7.5
92	6.5
93	4.0
94	4.0
95	4.0
96	2.0
97	2.0
98	3.5
99	5.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05977107677594812
2	0.009961846129324686
3	0.012344026725467548
4	0.009853565193136375
5	0.012603900972319495
6	0.012755494282983131
7	0.0029668976515597437
8	0.005630608681792214
9	0.007752915031083126
10-11	0.007449728409755853
12-13	0.00633443476701624
14-15	0.010947202648638323
16-17	0.010546563184741571
18-19	0.008489225397163645
20-21	0.007449728409755853
22-23	0.002642054842994808
24-25	0.003768176579353251
26-27	0.005056719719994162
28-29	0.006366919047872735
30-31	0.009604519039903258
32-33	0.011304529738059753
34-35	0.012852947125552612
36-37	0.019739614667129243
38-39	0.020031973194837686
40-41	0.01934980329685132
42-43	0.025987424685194833
44-45	0.029528211298552633
46-47	0.03208364139259679
48-49	0.03383779255884744
50-51	0.03430340058445718
52-53	0.03237599992030523
54-55	0.03862380993837082
56-57	0.041320005249459786
58-59	0.038645466125608485
60-61	0.03943591695978316
62-63	0.03464989958025978
64-65	0.03690214305297666
66-67	0.03960916645768446
68-69	0.04196969086658966
70-71	0.041742300900594206
72-73	0.03771425007438901
74-75	0.04038878919824031
76-77	0.04029133635567082
78-79	0.03986904070453641
80-81	0.04467671427129745
82-83	0.04057286678976044
84-85	0.03654481596355524
86-87	0.03490977382711173
88-89	0.03298237316295977
90-91	0.03528875710377082
92-93	0.036685581180600044
94-95	0.04017222732586368
96-97	0.03516964807396368
98-99	0.032668358448013676
100	0.03025369357101432
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4617618.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.35472319952197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.63009232871298	40.509693950700125
2	15.122125552391704	17.34650650491689
3	6.186846262459332	10.645345645840559
4	2.984364521877482	6.846696043150267
5	1.635849894652563	4.691185895188247
6	0.9967359878517255	3.4300510011742675
7	0.6578012891178474	2.640960760035
8	0.4153309913341276	1.9056955235321555
9	0.29406426335267355	1.5179376984717532
>10	1.0527816589496186	9.257005559814901
>50	0.018761928478739982	0.7153957539077869
>100	0.005245320821175044	0.4935256632680793
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.496856171298708E-5
2	0.0	0.0	0.0	0.0	6.496856171298708E-5
3	0.0	0.0	0.0	0.0	6.496856171298708E-5
4	0.0	0.0	0.0	0.0	6.496856171298708E-5
5	0.0	0.0	0.0	0.0	6.496856171298708E-5
6	0.0	0.0	0.0	0.0	1.2993712342597417E-4
7	0.0	0.0	0.0	0.0	1.2993712342597417E-4
8	0.0	0.0	0.0	0.0	1.5159331066363652E-4
9	0.0	0.0	0.0	0.0	2.1656187237662362E-4
10-11	2.1656187237662363E-5	0.0	0.0	0.0	2.1656187237662362E-4
12-13	2.1656187237662363E-5	0.0	0.0	0.0	2.4904615323311717E-4
14-15	2.1656187237662363E-5	0.0	0.0	0.0	3.464989958025978E-4
16-17	2.1656187237662363E-5	0.0	0.0	2.1656187237662363E-5	4.439518383720784E-4
18-19	2.1656187237662363E-5	0.0	0.0	3.248428085649355E-5	5.197484937038967E-4
20-21	2.1656187237662363E-5	0.0	0.0	5.4140468094155905E-5	5.305765873227278E-4
22-23	2.1656187237662363E-5	0.0	0.0	1.732494979012989E-4	6.063732426545461E-4
24-25	2.1656187237662363E-5	0.0	0.0	3.2484280856493543E-4	6.929979916051956E-4
26-27	2.1656187237662363E-5	0.0	0.0	6.929979916051956E-4	7.038260852240268E-4
28-29	2.1656187237662363E-5	0.0	0.0	0.0025554300940441588	7.363103660805203E-4
30-31	2.1656187237662363E-5	0.0	0.0	0.007558009345944164	7.471384596993514E-4
32-33	2.1656187237662363E-5	0.0	0.0	0.0211147825567208	7.79622740555845E-4
34-35	2.1656187237662363E-5	0.0	0.0	0.04277096979438316	8.337632086500009E-4
36-37	2.1656187237662363E-5	0.0	0.0	0.07525525065087671	8.554193958876633E-4
38-39	2.1656187237662363E-5	0.0	0.0	0.12167528799480598	8.879036767441568E-4
40-41	2.1656187237662363E-5	0.0	0.0	0.16990361697308007	8.879036767441568E-4
42-43	2.1656187237662363E-5	0.0	0.0	0.2175255727086996	9.095598639818191E-4
44-45	2.1656187237662363E-5	0.0	0.0	0.276506198650473	9.528722384571439E-4
46-47	2.1656187237662363E-5	0.0	0.0	0.34719199379420296	9.961846129324687E-4
48-49	2.1656187237662363E-5	0.0	0.0	0.4247319721986531	0.0010611531746454557
50-51	2.1656187237662363E-5	0.0	0.0	0.5002687532836194	0.001082809361883118
52-53	2.1656187237662363E-5	0.0	0.0	0.5753615825302136	0.0011910902980714299
54-55	2.1656187237662363E-5	0.0	0.0	0.6564531756416403	0.001321027421497404
56-57	2.1656187237662363E-5	0.0	0.0	0.7515671499894534	0.0013426836087350664
58-59	2.1656187237662363E-5	0.0	0.0	0.8558741758196542	0.00137516788959156
60-61	2.1656187237662363E-5	0.0	0.0	0.9630398183652265	0.0014076521704480536
62-63	4.3312374475324727E-5	0.0	0.0	1.0709850836513546	0.0014076521704480536
64-65	4.3312374475324727E-5	0.0	0.0	1.1838896158149073	0.0014076521704480536
66-67	4.3312374475324727E-5	0.0	0.0	1.3088133318953625	0.0014076521704480536
68-69	4.3312374475324727E-5	0.0	0.0	1.4457345757054827	0.0014184802640668849
70-71	4.3312374475324727E-5	0.0	0.0	1.59074440544887	0.001494276919398703
72-73	4.3312374475324727E-5	0.0	0.0	1.7351803462304591	0.0015809016683493524
74-75	4.3312374475324727E-5	0.0	0.0	1.8824099351657066	0.0015809016683493524
76-77	4.3312374475324727E-5	0.0	0.0	2.0443115909544707	0.0015917297619681835
78-79	4.3312374475324727E-5	0.0	0.0	2.2196075985497283	0.0016025578555870148
80-81	4.3312374475324727E-5	0.0	0.0	2.4073992261811177	0.001624214042824677
82-83	4.3312374475324727E-5	0.0	0.0	2.60034502637507	0.0017649792598694826
84-85	4.3312374475324727E-5	0.0	0.0	2.7911143797516385	0.0017758073534883136
86-87	4.3312374475324727E-5	0.0	0.0	2.9923869839384722	0.0017866354471071447
88	4.3312374475324727E-5	0.0	0.0	3.154873356782653	0.0018191197279636385
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6720	0.0	36.235188	1
GGTATCA	3995	0.0	35.652985	1
CAACGCA	6600	0.0	33.535583	5
ATCAACG	6640	0.0	33.334286	3
TCAACGC	7665	0.0	29.244207	4
TATCAAC	8160	0.0	28.910252	2
AACGCAG	7885	0.0	28.903479	6
ACGCAGA	8515	0.0	25.826561	7
CGCAGAG	8915	0.0	24.827242	8
GTACTAG	1840	0.0	24.01486	1
GTACATG	14160	0.0	23.70309	1
TACATGG	14040	0.0	22.961237	2
ACATGGG	14075	0.0	21.902502	3
CGACGAG	895	0.0	19.42624	24-25
GTGATCG	1270	0.0	19.24104	8
GTATAAG	2505	0.0	19.140905	1
GCAGAGT	10980	0.0	19.130842	9
CATGGGG	10940	0.0	19.072132	4
TAGTACT	2530	0.0	18.945814	4
TGATCGC	1290	0.0	18.578445	9
>>END_MODULE