FastQCFastQC Report
Mon 16 Jan 2017
ERR1146557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617618
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130800.2832629290686237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112530.24369707498541454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.1375601013336313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.058.882451
GTATCAA143950.044.064851
ATCAACG175100.035.628993
CAACGCA176850.035.2494625
TATCAAC185900.033.967142
TCAACGC186650.033.6002044
AACGCAG190600.033.0907976
ACGCAGA208300.029.895077
CGCAGAG213200.029.2520758
GCAGAGT239800.025.7916839
GTACATG134200.024.8954471
GTGGTAT24450.024.2496641
TACATGG133350.024.0998042
ACATGGG134500.023.0346643
GAGTACT170050.022.73171612-13
CAGAGTA231100.022.51233910-11
AGAGTAC216600.022.44631210-11
GTACTAG17250.021.277451
TGGTATC27850.021.118872
GTACTTT183200.020.88198914-15
Produced by FastQC (version 0.11.5)