FastQCFastQC Report
Mon 16 Jan 2017
ERR1146556_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146556_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4198110
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.039.081461
GTATCAA63050.034.89731
CAACGCA65900.030.8782315
ATCAACG67450.030.2383213
AACGCAG78100.026.7755376
TATCAAC81300.026.4743232
TCAACGC77500.026.3777334
GTACATG137650.026.1969181
TACATGG134250.025.6239872
ACATGGG137150.024.46543
ACGCAGA84250.024.2617847
CGCAGAG86050.023.5378068
GTACTAG18500.023.380141
CATGGGG97300.021.7344974
TGATCGC9550.020.6673839
TAACGCC5900.019.9130574
CTGTGCG21650.019.7525449
GTGATCG10050.019.6391548
CTAACGC6150.019.103583
GCAGAGT105000.018.663219
Produced by FastQC (version 0.11.5)