##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146556_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4198110
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253694638777926	31.0	31.0	34.0	28.0	34.0
2	31.441489860913602	31.0	31.0	34.0	28.0	34.0
3	31.544288977658994	31.0	31.0	34.0	28.0	34.0
4	35.17135449047309	37.0	35.0	37.0	32.0	37.0
5	35.193950611108335	37.0	35.0	37.0	32.0	37.0
6	35.3894471559821	37.0	35.0	37.0	33.0	37.0
7	35.36567598276367	37.0	35.0	37.0	33.0	37.0
8	35.28282203181908	37.0	35.0	37.0	33.0	37.0
9	36.97390730590671	39.0	38.0	39.0	33.0	39.0
10-11	36.93983911807932	39.0	37.5	39.0	33.0	39.0
12-13	36.90065064993533	39.0	37.5	39.0	33.0	39.0
14-15	38.325880813032526	40.5	39.0	41.0	33.0	41.0
16-17	38.33048705250697	40.5	38.5	41.0	33.0	41.0
18-19	38.35856599755604	40.0	38.5	41.0	33.5	41.0
20-21	38.452417873757476	41.0	39.0	41.0	34.0	41.0
22-23	38.468459020845096	40.5	39.0	41.0	34.0	41.0
24-25	38.52390599579334	41.0	39.0	41.0	34.0	41.0
26-27	38.5526873998061	41.0	39.0	41.0	34.0	41.0
28-29	38.51676516337113	40.0	39.0	41.0	34.0	41.0
30-31	38.50217871851857	40.0	38.5	41.0	33.5	41.0
32-33	38.51725740869105	40.0	38.0	41.0	33.5	41.0
34-35	38.466606996958156	40.0	38.0	41.0	33.0	41.0
36-37	38.38174309391607	40.0	38.0	41.0	33.0	41.0
38-39	38.34839344371633	40.0	38.0	41.0	33.0	41.0
40-41	38.294113065165035	40.0	38.0	41.0	33.0	41.0
42-43	38.179304853850894	40.0	38.0	41.0	33.0	41.0
44-45	38.05557619976609	40.0	38.0	41.0	33.0	41.0
46-47	37.96074328686004	40.0	38.0	41.0	33.0	41.0
48-49	37.85410827729621	40.0	37.5	41.0	32.0	41.0
50-51	37.742349414379326	40.0	37.0	41.0	32.0	41.0
52-53	37.628127538344636	40.0	37.0	41.0	32.0	41.0
54-55	37.45379325458361	40.0	36.0	41.0	32.0	41.0
56-57	37.33080136061228	40.0	36.0	41.0	31.0	41.0
58-59	37.12248642841659	39.0	35.5	41.0	31.0	41.0
60-61	36.944379256379655	39.0	35.0	41.0	31.0	41.0
62-63	36.70830218836572	39.0	35.0	41.0	31.0	41.0
64-65	36.464769622520606	38.0	35.0	40.0	31.0	41.0
66-67	36.197223393384164	37.5	35.0	40.0	30.0	41.0
68-69	35.893755404217615	37.0	35.0	40.0	30.0	41.0
70-71	35.61767831238343	36.5	35.0	39.0	30.0	41.0
72-73	35.28769958386036	36.0	35.0	39.0	30.0	41.0
74-75	34.95714130882707	35.5	34.0	39.0	30.0	40.5
76-77	34.63626727265364	35.0	34.0	37.5	29.0	39.5
78-79	34.345241191869675	35.0	34.0	37.0	29.0	39.0
80-81	34.048363549311475	35.0	34.0	36.5	29.0	39.0
82-83	33.78195830981085	35.0	34.0	36.0	29.0	38.0
84-85	33.54671471209663	35.0	34.0	36.0	29.0	37.0
86-87	33.343055803683086	35.0	34.0	35.5	29.0	37.0
88-89	33.18653715600592	35.0	34.0	35.0	28.5	36.0
90-91	33.0131422235244	35.0	34.0	35.0	28.0	36.0
92-93	32.914480682974	35.0	34.0	35.0	28.0	36.0
94-95	32.81435765141933	35.0	34.0	35.0	27.5	36.0
96-97	32.73348792670988	35.0	34.0	35.0	27.0	35.5
98-99	32.65830766702159	35.0	33.0	35.0	27.0	35.0
100	31.76460502464204	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09599104610598985
1101	2	0.10907173367491296
1101	3	0.16374826571561485
1101	4	0.2229516680249617
1101	5	0.1854238920049056
1101	6	0.19594541924814024
1101	7	0.25368766220461225
1101	8	0.2551352669918501
1101	9	0.3886028768065941
1101	10-11	0.4255229672610099
1101	12-13	0.4127561732920384
1101	14-15	0.518814045062399
1101	16-17	0.47363957374011534
1101	18-19	0.45835224326710744
1101	20-21	0.41530105725873057
1101	22-23	0.41024777750114083
1101	24-25	0.3865301202135356
1101	26-27	0.3770483986791362
1101	28-29	0.4048074084920472
1101	30-31	0.3958095351363866
1101	32-33	0.37564991813650295
1101	34-35	0.378804389607879
1101	36-37	0.379431645673705
1101	38-39	0.38514034408719766
1101	40-41	0.36342370429174764
1101	42-43	0.365210137669429
1101	44-45	0.2978425280569468
1101	46-47	0.2869777379670495
1101	48-49	0.30379007373014133
1101	50-51	0.31859620320695115
1101	52-53	0.3058475638439617
1101	54-55	0.3439153223205622
1101	56-57	0.25073544975508355
1101	58-59	0.32827469595459036
1101	60-61	0.2968822883456781
1101	62-63	0.2810635293309218
1101	64-65	0.3165919110473183
1101	66-67	0.3008126939220972
1101	68-69	0.26078024249345333
1101	70-71	0.20128240058008373
1101	72-73	0.23822130239638284
1101	74-75	0.2110365490631807
1101	76-77	0.2290672557121809
1101	78-79	0.30340754052986796
1101	80-81	0.2996223222422145
1101	82-83	0.22380078961668204
1101	84-85	0.28295194930957024
1101	86-87	0.24116889315047985
1101	88-89	0.16658921644381763
1101	90-91	0.2608225956953376
1101	92-93	0.254564729207857
1101	94-95	0.22572738648010215
1101	96-97	0.24757346634121546
1101	98-99	0.18982214490823424
1101	100	0.09237109161988855
1102	1	0.22157685780398495
1102	2	0.22323266615289938
1102	3	0.21879472688177515
1102	4	0.2269738230965359
1102	5	0.2727334932488361
1102	6	0.3289900312996892
1102	7	0.28815226588532994
1102	8	0.3027351091523869
1102	9	0.41462045078124277
1102	10-11	0.42477017396065975
1102	12-13	0.4881153646633578
1102	14-15	0.5483237104544259
1102	16-17	0.4534867937342426
1102	18-19	0.485843707835123
1102	20-21	0.49155662781889475
1102	22-23	0.4905264482374463
1102	24-25	0.55306257036181
1102	26-27	0.4940792987333822
1102	28-29	0.5267712644025195
1102	30-31	0.5073828587825133
1102	32-33	0.516972858726497
1102	34-35	0.5078495465997293
1102	36-37	0.5370763323465155
1102	38-39	0.44141666634363474
1102	40-41	0.47001891708501375
1102	42-43	0.46649067736755256
1102	44-45	0.44124014822060786
1102	46-47	0.4329290482092816
1102	48-49	0.5000985687466653
1102	50-51	0.43962228784835133
1102	52-53	0.3964432412086367
1102	54-55	0.43314716126969444
1102	56-57	0.46427705170103906
1102	58-59	0.47771041426400274
1102	60-61	0.47069942958144395
1102	62-63	0.38354940990603836
1102	64-65	0.40087845527487076
1102	66-67	0.4475522738903166
1102	68-69	0.41534653102610974
1102	70-71	0.41450238693767005
1102	72-73	0.3898046308495964
1102	74-75	0.39357602546894555
1102	76-77	0.3661942645013383
1102	78-79	0.345807644813398
1102	80-81	0.38527674783267685
1102	82-83	0.34667752904228166
1102	84-85	0.31042885226626993
1102	86-87	0.3343686836733042
1102	88-89	0.34009587332293023
1102	90-91	0.3704948239757684
1102	92-93	0.40029772029198796
1102	94-95	0.2788006267995087
1102	96-97	0.3071304041141616
1102	98-99	0.33929242954258143
1102	100	0.28479833965702284
1103	1	0.17605039039939996
1103	2	0.13711178270089874
1103	3	0.18614571673998626
1103	4	0.24875070037515457
1103	5	0.22261741261355894
1103	6	0.298704029332292
1103	7	0.2658946963452351
1103	8	0.29914231900455945
1103	9	0.4380550651405457
1103	10-11	0.4371704390666693
1103	12-13	0.4161161920342522
1103	14-15	0.5178907882274615
1103	16-17	0.4687741067264284
1103	18-19	0.4439170307877305
1103	20-21	0.4484157173400263
1103	22-23	0.46510180560907344
1103	24-25	0.4426626049859195
1103	26-27	0.42779473692444014
1103	28-29	0.45681341003804476
1103	30-31	0.3962005527001722
1103	32-33	0.40792209956893544
1103	34-35	0.418021463328067
1103	36-37	0.4417903911261263
1103	38-39	0.4169705049033965
1103	40-41	0.39894456298137726
1103	42-43	0.3826637255047203
1103	44-45	0.40419053050103315
1103	46-47	0.3714879918399987
1103	48-49	0.3266749612069333
1103	50-51	0.29864793207206475
1103	52-53	0.3226986606075073
1103	54-55	0.40720058235783796
1103	56-57	0.3569668037344371
1103	58-59	0.35896567767262866
1103	60-61	0.25061059221290094
1103	62-63	0.28508180621871304
1103	64-65	0.26507945861353477
1103	66-67	0.30434236086690447
1103	68-69	0.21894241405490078
1103	70-71	0.18679685061007234
1103	72-73	0.2070740430275464
1103	74-75	0.2647413621014465
1103	76-77	0.22196336421080787
1103	78-79	0.24419804090308617
1103	80-81	0.29788270130208616
1103	82-83	0.2637436760062286
1103	84-85	0.19242314258588777
1103	86-87	0.17218673927967387
1103	88-89	0.2036882623049152
1103	90-91	0.2401302614856533
1103	92-93	0.23785946053999396
1103	94-95	0.1762051039786101
1103	96-97	0.13503840130479716
1103	98-99	0.12642622646252732
1103	100	0.1477599862166521
1104	1	0.08091617060927803
1104	2	0.14350405073750494
1104	3	0.09403466665958504
1104	4	0.1495346384044609
1104	5	0.11224545753614734
1104	6	0.1803269806148151
1104	7	0.18125364460929916
1104	8	0.25470579571870644
1104	9	0.2698380188357774
1104	10-11	0.24789364868875907
1104	12-13	0.2807015653689433
1104	14-15	0.32148110504955696
1104	16-17	0.28874676803465604
1104	18-19	0.26199797718579276
1104	20-21	0.2720626269545363
1104	22-23	0.28713398062804174
1104	24-25	0.29008323583240525
1104	26-27	0.2737026439375967
1104	28-29	0.290931798989881
1104	30-31	0.2588359628030119
1104	32-33	0.2961072833519367
1104	34-35	0.2848972421647531
1104	36-37	0.2894756339434963
1104	38-39	0.2650532351663415
1104	40-41	0.22235150106073576
1104	42-43	0.2150006077452602
1104	44-45	0.2172392293705201
1104	46-47	0.17095563971790995
1104	48-49	0.2311107369379073
1104	50-51	0.18823215038632668
1104	52-53	0.20866949377506927
1104	54-55	0.2034372210173956
1104	56-57	0.1517707358089737
1104	58-59	0.17426629740525357
1104	60-61	0.17963771530085637
1104	62-63	0.2038222532372913
1104	64-65	0.16928543340585378
1104	66-67	0.12835069562262902
1104	68-69	0.1436905393925798
1104	70-71	0.1124007177467945
1104	72-73	0.11743250682471285
1104	74-75	0.16240738666230925
1104	76-77	0.18923119227189744
1104	78-79	0.15728427793637678
1104	80-81	0.15381313763029425
1104	82-83	0.12856358265491252
1104	84-85	0.17664392730676326
1104	86-87	0.17642523110533403
1104	88-89	0.13007027632685464
1104	90-91	0.15052916703962893
1104	92-93	0.15122266184594935
1104	94-95	0.08509354761425669
1104	96-97	0.0820311612517628
1104	98-99	0.056662190718498096
1104	100	0.029805803310182455
1105	1	0.12196196691823147
1105	2	0.16880798656775653
1105	3	0.14098230965985792
1105	4	0.14208273358413237
1105	5	0.09712373715127143
1105	6	0.1272825104650792
1105	7	0.1704311337867921
1105	8	0.21297004608822334
1105	9	0.30493566972290154
1105	10-11	0.30326926722226943
1105	12-13	0.29396739310845277
1105	14-15	0.31503316702267625
1105	16-17	0.2941759149111718
1105	18-19	0.31015040954875417
1105	20-21	0.32646911228208353
1105	22-23	0.2866284919646489
1105	24-25	0.3530177428458643
1105	26-27	0.2920824366594985
1105	28-29	0.36121509756265624
1105	30-31	0.36472743974712074
1105	32-33	0.2868874826847687
1105	34-35	0.27801479712441335
1105	36-37	0.2965318817892211
1105	38-39	0.26865957035162324
1105	40-41	0.2810118465631888
1105	42-43	0.3186739811554773
1105	44-45	0.2561921624023995
1105	46-47	0.24811335042824822
1105	48-49	0.24780727139759762
1105	50-51	0.27287411660724104
1105	52-53	0.2062095268414268
1105	54-55	0.29737887877484326
1105	56-57	0.33245766583800673
1105	58-59	0.2906309269277756
1105	60-61	0.30639232034493347
1105	62-63	0.2805663424813787
1105	64-65	0.24746850764156392
1105	66-67	0.289015688524465
1105	68-69	0.2670428449817308
1105	70-71	0.16806515564806546
1105	72-73	0.2281443826681766
1105	74-75	0.31582677429221917
1105	76-77	0.31165547851197317
1105	78-79	0.27057077214391967
1105	80-81	0.3043997586116518
1105	82-83	0.2431809827986271
1105	84-85	0.23711956278240365
1105	86-87	0.22167230858925535
1105	88-89	0.19530299682273977
1105	90-91	0.2329701179016368
1105	92-93	0.2617460565798666
1105	94-95	0.2137579387786417
1105	96-97	0.2320259763781607
1105	98-99	0.2341556037382304
1105	100	0.18889598213720404
1106	1	0.07146496485656684
1106	2	0.05754051898545853
1106	3	0.04295612002005811
1106	4	0.05087335429720241
1106	5	0.13607491287313422
1106	6	0.10835336842524868
1106	7	0.1522960227177066
1106	8	0.1509271070897782
1106	9	0.19022130921741365
1106	10-11	0.1872335079028531
1106	12-13	0.18060502530466493
1106	14-15	0.22936400487354547
1106	16-17	0.236155360187162
1106	18-19	0.22341169171882314
1106	20-21	0.2255523463200717
1106	22-23	0.23646959867538442
1106	24-25	0.257760265488173
1106	26-27	0.20340829944376537
1106	28-29	0.212325028051076
1106	30-31	0.2055091059762404
1106	32-33	0.24282823367415318
1106	34-35	0.15273632431377848
1106	36-37	0.19989799744794112
1106	38-39	0.16105959518064594
1106	40-41	0.21164773353916644
1106	42-43	0.1814131419262779
1106	44-45	0.1723830616263058
1106	46-47	0.10653762557914348
1106	48-49	0.13274336915758056
1106	50-51	0.12586530161372167
1106	52-53	0.14864370539829963
1106	54-55	0.18757029586907237
1106	56-57	0.08933038987326114
1106	58-59	0.22157278043074768
1106	60-61	0.16297992538268602
1106	62-63	0.13576204313463336
1106	64-65	0.1371250362984071
1106	66-67	0.09925595906678097
1106	68-69	0.17674648518433145
1106	70-71	0.17012069717163314
1106	72-73	0.14875689359135436
1106	74-75	0.12520136084962985
1106	76-77	0.14516843515477973
1106	78-79	0.1328642271118028
1106	80-81	0.15285883171777925
1106	82-83	0.1422208628230308
1106	84-85	0.11635041889559972
1106	86-87	0.1223611894875134
1106	88-89	0.07297659225154263
1106	90-91	0.10425022671800122
1106	92-93	0.11109474409101949
1106	94-95	0.12773212903945108
1106	96-97	0.06471358928212112
1106	98-99	0.07674423096850091
1106	100	0.1975888765996281
1107	1	0.06439333174601458
1107	2	0.031913513615052125
1107	3	0.09433596110981313
1107	4	0.10913142459698832
1107	5	0.10072931410511643
1107	6	0.11211912464100493
1107	7	0.10050673194749749
1107	8	0.13596602485127818
1107	9	0.22109527222069403
1107	10-11	0.218351913827874
1107	12-13	0.18363639649525965
1107	14-15	0.25544926454963246
1107	16-17	0.250930594846146
1107	18-19	0.2012778462528999
1107	20-21	0.2174632369828089
1107	22-23	0.24140789819982444
1107	24-25	0.2304399535827386
1107	26-27	0.19361969497167308
1107	28-29	0.18336410073069231
1107	30-31	0.17104396756420925
1107	32-33	0.22787524942841486
1107	34-35	0.19010852024836566
1107	36-37	0.18077529173251605
1107	38-39	0.21697259240485067
1107	40-41	0.19846368674738812
1107	42-43	0.2234628901437148
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2216	52-53	-0.32464870876250274
2216	54-55	-0.357689992257896
2216	56-57	-0.35010038406160504
2216	58-59	-0.31510503388771127
2216	60-61	-0.3618185684685855
2216	62-63	-0.3510399437627143
2216	64-65	-0.3637862083397536
2216	66-67	-0.37086899718909905
2216	68-69	-0.33758036499629185
2216	70-71	-0.3398455288715141
2216	72-73	-0.33191782388218627
2216	74-75	-0.31060382633560124
2216	76-77	-0.3575232731804121
2216	78-79	-0.3634443660765214
2216	80-81	-0.30816232341850736
2216	82-83	-0.234925821277713
2216	84-85	-0.22056269664130213
2216	86-87	-0.20227321248759011
2216	88-89	-0.27924249942079626
2216	90-91	-0.27341898542466225
2216	92-93	-0.285666908856669
2216	94-95	-0.2668790550038267
2216	96-97	-0.24614766953167333
2216	98-99	-0.2181280359855009
2216	100	-0.3252779114996187
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	115.0
1	53.0
2	44.0
3	64.0
4	44.0
5	61.0
6	162.0
7	814.0
8	2311.0
9	2838.0
10	2687.0
11	3286.0
12	4124.0
13	5275.0
14	6488.0
15	7262.0
16	8071.0
17	9274.0
18	10046.0
19	11565.0
20	13048.0
21	14896.0
22	17025.0
23	19750.0
24	24279.0
25	30636.0
26	35687.0
27	34057.0
28	36488.0
29	46317.0
30	53941.0
31	59025.0
32	75814.0
33	102800.0
34	149553.0
35	229953.0
36	435290.0
37	991664.0
38	1328780.0
39	423613.0
40	910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.382758266454786	20.012603857285626	13.275594255901682	24.329043620357897
2	15.011489630745231	20.971279972975665	40.36033559346099	23.656894802818115
3	17.900833545954377	26.083801516530002	30.662789221197116	25.352575716318505
4	12.046454010529576	17.845677394763797	40.05803177930772	30.049836815398912
5	13.174745848757826	38.44836059852311	35.62791491040473	12.748978642314333
6	29.66762745172311	38.74670080038422	18.83083917534959	12.754832572543084
7	26.235238488850033	32.956875841919775	23.37925604961416	17.428629619616032
8	25.050793459465165	35.42530842331718	21.458673336060105	18.06522478115755
9	26.15744143083962	15.473049669887788	20.87814732188055	37.49136157739205
10-11	24.0547079654387	26.585011517297147	29.547587418363808	19.812693098900343
12-13	24.649454607910737	24.520805464414355	28.721359009483784	22.108380918191127
14-15	22.255845244260765	25.84664503252506	26.121332086297073	25.7761776369171
16-17	22.010756116825277	28.50225641180793	26.988369476698516	22.498617994668276
18-19	21.897202496595536	28.360736400633048	28.07546768882198	21.666593413949435
20-21	23.224402117044924	27.661596788342557	27.32924670052938	21.784754394083137
22-23	23.072122252588557	27.56163236896072	26.9440997694853	22.42214560896542
24-25	22.675570051506043	27.692514019924946	27.364377980320892	22.267537948248123
26-27	22.537813504593363	27.919223993873132	27.02313415858625	22.519828342947257
28-29	22.47911856187916	28.017860022721063	27.14881152737807	22.354209888021703
30-31	22.797930361788854	27.713076934163478	27.103046080645566	22.385946623402102
32-33	22.674706208934793	27.964571285226548	26.832847805871285	22.527874699967374
34-35	22.93840592269023	27.92651882811198	27.046269915603354	22.088805333594433
36-37	22.873024935772182	27.62014774274375	26.998031025904712	22.508796295579355
38-39	22.6380786856422	27.81953310850814	27.182843399442742	22.359544806406923
40-41	22.81559354192988	27.500260283087936	27.318097858340725	22.366048316641464
42-43	22.73949703766648	27.460300990192426	27.16214369709087	22.63805827505022
44-45	22.64187695104225	27.84680466590604	27.14074523782775	22.37057314522396
46-47	22.702154417129353	27.59822448465117	26.966479272866945	22.733141825352536
48-49	22.708932000895917	27.517763449468884	27.214200275446647	22.559104274188552
50-51	22.687375745349193	27.529546798380515	27.024790109642538	22.75828734662775
52-53	22.58449136483109	27.609499412535456	27.04452793035918	22.761481292274276
54-55	22.625598881373236	27.61838585474723	26.980258182031342	22.775757081848194
56-57	22.8448512616349	27.508867849825076	26.950435478119218	22.695845410420805
58-59	22.5533007473334	27.547394162518952	27.26558185668713	22.633723233460522
60-61	22.685927194689366	27.615152593956672	27.078117754485696	22.620802456868265
62-63	22.650350264376662	27.360962037725066	27.07115503548751	22.917532662410764
64-65	22.411367121273678	27.47105141820946	27.108585031209305	23.008996429307558
66-67	22.686811571807063	27.42410661680998	26.902262721516173	22.986819089866785
68-69	22.48701935759373	27.372182761535313	27.140236214449338	23.000561666421618
70-71	22.541178393774857	27.143407435755773	27.072955306565653	23.242458863903714
72-73	22.67924390725125	27.194797823395355	27.088879452741583	23.037078816611814
74-75	22.58292047150485	27.44830777684658	27.014674774652626	22.954096976995945
76-77	22.52979397847387	27.27844628684073	27.039832337569575	23.15192739711583
78-79	22.53194871049452	27.289286250437854	26.981296630343355	23.197468408724266
80-81	22.515559921245995	27.363144421901087	27.01336492263053	23.10793073422239
82-83	22.777685174583365	27.068812619600603	27.375864689774467	22.77763751604157
84-85	22.796384234048062	27.347378336166727	26.907589851159603	22.94864757862561
86-87	22.3358907460515	27.49630696768181	27.130560055395186	23.0372422308715
88-89	22.762444656428755	27.106284318137057	27.027129742379497	23.10414128305469
90-91	22.668068385750264	27.03816273134113	27.14598422136741	23.147784661541195
92-93	22.801340731111402	27.046663595180508	27.170130666835373	22.981865006872717
94-95	22.396614052046214	27.051244000833545	27.32202785617275	23.230114090947488
96-97	22.509489531134953	27.0287104483729	27.277523214502718	23.184276805989427
98-99	22.386910827972358	27.229026675527297	27.067166915703066	23.31689558079728
100	22.715435120949795	26.936859832430613	26.90271597360749	23.444989073012103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1216.0
1	878.0
2	586.0
3	598.0
4	520.0
5	454.5
6	399.5
7	383.5
8	438.0
9	479.5
10	430.5
11	373.0
12	372.0
13	400.0
14	462.0
15	543.5
16	595.0
17	722.0
18	1001.5
19	1359.5
20	1920.0
21	2819.5
22	4251.0
23	6488.0
24	9220.5
25	12759.0
26	18599.0
27	25382.0
28	31502.0
29	39029.5
30	48910.5
31	59024.5
32	70160.5
33	80843.0
34	90168.0
35	98978.0
36	109168.0
37	121719.0
38	128300.5
39	130221.0
40	131226.0
41	131905.0
42	134315.0
43	140545.0
44	147585.5
45	156786.0
46	165758.5
47	170359.0
48	172060.5
49	168627.5
50	164624.0
51	162316.0
52	157749.0
53	152056.0
54	144580.5
55	131885.0
56	120217.5
57	105275.5
58	89906.5
59	79713.5
60	65487.0
61	51352.5
62	40514.0
63	31019.0
64	23094.5
65	16208.5
66	11407.0
67	7971.5
68	5931.0
69	4418.0
70	3152.0
71	2255.0
72	1631.5
73	1306.5
74	1032.5
75	763.5
76	561.5
77	406.0
78	274.5
79	175.5
80	128.5
81	85.0
82	56.0
83	39.5
84	39.0
85	37.5
86	28.5
87	24.5
88	23.5
89	19.0
90	16.5
91	14.5
92	14.0
93	10.5
94	7.0
95	5.5
96	6.0
97	8.0
98	7.0
99	6.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06119420405849299
2	0.009813940082560962
3	0.01193394170233748
4	0.009766299596723286
5	0.012172144131525854
6	0.01193394170233748
7	0.002739327935666288
8	0.005407195142576064
9	0.007026971661057
10-11	0.006991241296678744
12-13	0.006157532794519439
14-15	0.01009978299758701
16-17	0.00965910850358852
18-19	0.00746764615505549
20-21	0.006610117409977347
22-23	0.0026916874498286135
24-25	0.003715957895338617
26-27	0.004883149798361643
28-29	0.005788319029277461
30-31	0.008849220244348052
32-33	0.010516637248666662
34-35	0.01168382915168969
36-37	0.017781811338912032
38-39	0.018460688262098898
40-41	0.018031923889559827
42-43	0.023736872068621358
44-45	0.027500470449797646
46-47	0.029632382191033584
48-49	0.0307281133653001
50-51	0.031811934418107195
52-53	0.030037326320653816
54-55	0.03658789312333407
56-57	0.038838906079164195
58-59	0.03626631984392977
60-61	0.03687373603836012
62-63	0.03219305830480859
64-65	0.03442025101771988
66-67	0.036885646159819534
68-69	0.03975598543153943
70-71	0.039148569237109083
72-73	0.03530160000571686
74-75	0.03785036599803245
76-77	0.037695534419060005
78-79	0.03668317409500942
80-81	0.04173306559380292
82-83	0.03788609636241071
84-85	0.034146318224153246
86-87	0.03228833927648394
88-89	0.029977775713356726
90-91	0.032359800005240455
92-93	0.03440834089626046
94-95	0.03786227611949187
96-97	0.032871935227995454
98-99	0.030180247778166844
100	0.02767912227168893
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4198110.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.856595628040196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43318741628684	40.0459125572179
2	15.511464875745931	17.638581720776703
3	6.059146253316693	10.335072851279461
4	2.9798047781576007	6.776862212888349
5	1.6313013541797334	4.637512072103574
6	0.995678002070151	3.396651692366253
7	0.6330952662626802	2.5196949083546554
8	0.4000966014094255	1.8198504542791107
9	0.2791374145781094	1.4283722794791764
>10	1.0344383299821929	9.125317542593734
>50	0.03249865511153296	1.250806100994132
>100	0.009934239894159107	0.9468410293061255
>500	2.1681300496518524E-4	0.07852457836073652
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.764048583767457E-5	0.0
3	0.0	0.0	0.0	4.764048583767457E-5	0.0
4	0.0	0.0	0.0	4.764048583767457E-5	0.0
5	0.0	0.0	0.0	4.764048583767457E-5	0.0
6	0.0	0.0	0.0	4.764048583767457E-5	0.0
7	0.0	0.0	0.0	4.764048583767457E-5	0.0
8	0.0	0.0	0.0	4.764048583767457E-5	0.0
9	0.0	0.0	0.0	4.764048583767457E-5	0.0
10-11	0.0	0.0	0.0	1.429214575130237E-4	0.0
12-13	0.0	0.0	0.0	1.429214575130237E-4	0.0
14-15	0.0	0.0	0.0	1.429214575130237E-4	0.0
16-17	0.0	0.0	0.0	1.429214575130237E-4	0.0
18-19	0.0	0.0	0.0	1.429214575130237E-4	0.0
20-21	0.0	0.0	0.0	1.429214575130237E-4	1.1910121459418643E-5
22-23	0.0	0.0	0.0	1.429214575130237E-4	2.3820242918837286E-5
24-25	0.0	0.0	0.0	4.2876437253907114E-4	2.3820242918837286E-5
26-27	0.0	0.0	0.0	0.001036180566969422	2.3820242918837286E-5
28-29	0.0	0.0	0.0	0.0032157327940430334	2.3820242918837286E-5
30-31	0.0	0.0	0.0	0.010337985426775383	2.3820242918837286E-5
32-33	0.0	0.0	0.0	0.027500470449797646	4.764048583767457E-5
34-35	0.0	0.0	0.0	0.05623959353137483	4.764048583767457E-5
36-37	0.0	0.0	0.0	0.10192681944970475	4.764048583767457E-5
38-39	0.0	0.0	0.0	0.16490754172711053	4.764048583767457E-5
40-41	0.0	0.0	0.0	0.22887680408564806	4.764048583767457E-5
42-43	0.0	0.0	0.0	0.2940608988330463	4.764048583767457E-5
44-45	0.0	0.0	0.0	0.37329893690255855	5.955060729709321E-5
46-47	0.0	0.0	0.0	0.4704021571611987	7.146072875651185E-5
48-49	0.0	0.0	0.0	0.5719716729671209	7.146072875651185E-5
50-51	0.0	0.0	0.0	0.6736841102305562	9.528097167534915E-5
52-53	0.0	0.0	0.0	0.7718949717849222	1.1910121459418643E-4
54-55	0.0	0.0	0.0	0.8770017936642918	1.1910121459418643E-4
56-57	0.0	0.0	0.0	1.0021176195954846	1.1910121459418643E-4
58-59	0.0	0.0	0.0	1.1427285135453813	1.1910121459418643E-4
60-61	0.0	0.0	0.0	1.284744801827489	1.1910121459418643E-4
62-63	0.0	0.0	0.0	1.4213777152099398	1.429214575130237E-4
64-65	0.0	0.0	0.0	1.562810407540536	1.429214575130237E-4
66-67	0.0	0.0	0.0	1.7215723265945866	1.429214575130237E-4
68-69	0.0	0.0	0.0	1.8993785298622474	1.429214575130237E-4
70-71	0.0	0.0	0.0	2.0810197922398412	1.429214575130237E-4
72-73	0.0	0.0	0.0	2.2665794845775835	1.5483157897244236E-4
74-75	0.0	0.0	0.0	2.456879405256175	1.66741700431861E-4
76-77	0.0	0.0	0.0	2.6589703461795904	1.66741700431861E-4
78-79	0.0	0.0	0.0	2.8765563551217097	1.66741700431861E-4
80-81	0.0	0.0	0.0	3.1135915924070594	1.905619433506983E-4
82-83	0.0	0.0	0.0	3.35797537463287	2.1438218626953557E-4
84-85	0.0	0.0	0.0	3.6011085941054426	2.1438218626953557E-4
86-87	0.0	1.1910121459418643E-5	0.0	3.8541629447537105	2.262923077289542E-4
88	0.0	2.3820242918837286E-5	0.0	4.056563548835071	2.3820242918837285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4030	0.0	39.08146	1
GTATCAA	6305	0.0	34.8973	1
CAACGCA	6590	0.0	30.878231	5
ATCAACG	6745	0.0	30.238321	3
AACGCAG	7810	0.0	26.775537	6
TATCAAC	8130	0.0	26.474323	2
TCAACGC	7750	0.0	26.377733	4
GTACATG	13765	0.0	26.196918	1
TACATGG	13425	0.0	25.623987	2
ACATGGG	13715	0.0	24.4654	3
ACGCAGA	8425	0.0	24.261784	7
CGCAGAG	8605	0.0	23.537806	8
GTACTAG	1850	0.0	23.38014	1
CATGGGG	9730	0.0	21.734497	4
TGATCGC	955	0.0	20.667383	9
TAACGCC	590	0.0	19.913057	4
CTGTGCG	2165	0.0	19.752544	9
GTGATCG	1005	0.0	19.639154	8
CTAACGC	615	0.0	19.10358	3
GCAGAGT	10500	0.0	18.66321	9
>>END_MODULE