FastQCFastQC Report
Mon 16 Jan 2017
ERR1146556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4198110
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124300.2960856194811475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114010.2715745895176639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61960.14759022512511583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81100.061.325351
GTATCAA145700.042.2409931
ATCAACG180600.033.5286873
CAACGCA181250.033.4343835
TATCAAC189650.032.251932
TCAACGC191950.031.717584
AACGCAG194600.031.3725786
ACGCAGA210500.028.7339157
CGCAGAG214100.028.2288158
GCAGAGT240250.024.9801989
GTACTAG19000.024.5170121
GAGTACT164650.024.01932112-13
TACATGG129700.023.9424842
GTACATG136800.023.6984021
GTGGTAT25400.023.5264241
CAGAGTA232700.023.09447910-11
ACATGGG133600.022.5564083
AGAGTAC221100.021.78730410-11
GTACTTT181300.021.65793414-15
TGGTATC27850.021.2868082
Produced by FastQC (version 0.11.5)