##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4198110
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.524405029882494	34.0	31.0	34.0	31.0	34.0
2	32.5610000690787	34.0	31.0	34.0	31.0	34.0
3	32.69283844396645	34.0	31.0	34.0	31.0	34.0
4	36.171708935687725	37.0	37.0	37.0	35.0	37.0
5	36.198085328874186	37.0	37.0	37.0	35.0	37.0
6	36.277151384789825	37.0	37.0	37.0	35.0	37.0
7	36.260060122293126	37.0	37.0	37.0	35.0	37.0
8	36.26762352582472	37.0	37.0	37.0	35.0	37.0
9	38.07356381800382	39.0	38.0	39.0	37.0	39.0
10-11	38.04521356038789	39.0	38.0	39.0	36.0	39.0
12-13	38.02659684953467	39.0	38.0	39.0	35.0	39.0
14-15	39.48052385478228	41.0	39.0	41.0	37.0	41.0
16-17	39.47376914849778	41.0	39.0	41.0	36.5	41.0
18-19	39.528426125089624	41.0	39.5	41.0	37.0	41.0
20-21	39.56396092527352	41.0	40.0	41.0	37.0	41.0
22-23	39.52405582512131	41.0	39.0	41.0	37.0	41.0
24-25	39.51592085486088	41.0	39.0	41.0	37.0	41.0
26-27	39.44334009828232	41.0	39.0	41.0	36.0	41.0
28-29	39.36238045215585	41.0	39.0	41.0	36.0	41.0
30-31	39.26469077751655	41.0	39.0	41.0	36.0	41.0
32-33	39.15018174845347	41.0	39.0	41.0	35.0	41.0
34-35	39.07837741269285	41.0	39.0	41.0	35.0	41.0
36-37	38.99867273606456	41.0	39.0	41.0	35.0	41.0
38-39	38.904502264114086	41.0	39.0	41.0	35.0	41.0
40-41	38.82067537534748	40.0	39.0	41.0	35.0	41.0
42-43	38.728832855737465	40.0	38.0	41.0	35.0	41.0
44-45	38.586156270321645	40.0	38.0	41.0	35.0	41.0
46-47	38.36163047180755	40.0	38.0	41.0	34.5	41.0
48-49	38.153726438802224	40.0	38.0	41.0	34.0	41.0
50-51	38.01262282312755	40.0	38.0	41.0	34.0	41.0
52-53	37.88910462088892	40.0	37.5	41.0	33.0	41.0
54-55	37.758330058049935	40.0	37.0	41.0	33.0	41.0
56-57	37.574439926538375	40.0	37.0	41.0	33.0	41.0
58-59	37.38190268954362	40.0	36.0	41.0	33.0	41.0
60-61	37.20805695896487	39.0	36.0	41.0	33.0	41.0
62-63	36.96019161003404	39.0	35.0	41.0	32.5	41.0
64-65	36.675158940570874	39.0	35.0	41.0	32.0	41.0
66-67	36.41512906998625	38.0	35.0	40.5	32.0	41.0
68-69	36.09470654651736	37.0	35.0	40.0	31.5	41.0
70-71	35.8043130122841	37.0	35.0	39.5	31.0	41.0
72-73	35.47165379182537	36.0	35.0	39.0	31.0	41.0
74-75	35.135397000078605	36.0	35.0	39.0	31.0	41.0
76-77	34.81826452856166	35.0	35.0	37.5	31.0	39.5
78-79	34.5208616496471	35.0	35.0	37.0	31.0	39.0
80-81	34.24693099990233	35.0	35.0	37.0	30.5	39.0
82-83	33.984798040070416	35.0	34.5	36.0	30.0	38.0
84-85	33.770299491914216	35.0	34.0	36.0	30.0	37.0
86-87	33.57787980781828	35.0	34.0	36.0	30.0	37.0
88-89	33.42091905643254	35.0	34.0	35.0	30.0	36.5
90-91	33.3293872718914	35.0	34.0	35.0	30.0	36.0
92-93	33.238618926135814	35.0	34.0	35.0	30.0	36.0
94-95	33.162401533070835	35.0	34.0	35.0	30.0	36.0
96-97	33.115178973395174	35.0	34.0	35.0	30.0	36.0
98-99	33.064554883030695	35.0	34.0	35.0	30.0	35.0
100	32.51972840159024	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22933138435882938
1101	2	0.19346321209646788
1101	3	0.2234937282586955
1101	4	0.1393305397132849
1101	5	0.1747822027707926
1101	6	0.11947546870036518
1101	7	0.13173007930664227
1101	8	0.11706238536859814
1101	9	0.15112907779576545
1101	10-11	0.17350003451778662
1101	12-13	0.20608649886361263
1101	14-15	0.31310090315583494
1101	16-17	0.2743057009271581
1101	18-19	0.2233186956951414
1101	20-21	0.24271628979217752
1101	22-23	0.22282812141998676
1101	24-25	0.24467350055003578
1101	26-27	0.25724513977856844
1101	28-29	0.3078788846730518
1101	30-31	0.2776996849656683
1101	32-33	0.24466758121619137
1101	34-35	0.30519296273077146
1101	36-37	0.32962917583968476
1101	38-39	0.32952048067689077
1101	40-41	0.2767458676644097
1101	42-43	0.28536391294776564
1101	44-45	0.3256066686020631
1101	46-47	0.32826736636791054
1101	48-49	0.33382428628412697
1101	50-51	0.35239060465321614
1101	52-53	0.32061353722718167
1101	54-55	0.2531174742052258
1101	56-57	0.30192075721770806
1101	58-59	0.33739160380484634
1101	60-61	0.3046835407120767
1101	62-63	0.3760393430343427
1101	64-65	0.2949778368521194
1101	66-67	0.3153770132803615
1101	68-69	0.31020479768964293
1101	70-71	0.26481256378678353
1101	72-73	0.2921860327084005
1101	74-75	0.27956457187665507
1101	76-77	0.26084767105201223
1101	78-79	0.23998410279472182
1101	80-81	0.2608543818969906
1101	82-83	0.28623064719138114
1101	84-85	0.26790372557714903
1101	86-87	0.2229681528537668
1101	88-89	0.2397188388792344
1101	90-91	0.2212722671642524
1101	92-93	0.22035848939692215
1101	94-95	0.2214093686054852
1101	96-97	0.13738402450987763
1101	98-99	0.17882105038105323
1101	100	0.1478351174763617
1102	1	0.25303226197944184
1102	2	0.25615385412946523
1102	3	0.24676675353530442
1102	4	0.20324729742210934
1102	5	0.18866666415586053
1102	6	0.1854144351767033
1102	7	0.19067432678234297
1102	8	0.22329784585348023
1102	9	0.2429828468745825
1102	10-11	0.23147815758699153
1102	12-13	0.250546126043659
1102	14-15	0.3154752508892784
1102	16-17	0.3118054204586187
1102	18-19	0.3307387047562429
1102	20-21	0.2713126516226012
1102	22-23	0.3284518717160765
1102	24-25	0.3036158403146416
1102	26-27	0.3114678897969725
1102	28-29	0.3738075075048073
1102	30-31	0.3771596907126096
1102	32-33	0.34812548435046864
1102	34-35	0.2996732544474554
1102	36-37	0.3015100918224505
1102	38-39	0.32596839262058097
1102	40-41	0.3655512019186702
1102	42-43	0.30075383841997194
1102	44-45	0.3165177378664268
1102	46-47	0.31625265437995154
1102	48-49	0.2664873351137942
1102	50-51	0.24702537546377812
1102	52-53	0.30280442405976515
1102	54-55	0.30375649266581206
1102	56-57	0.28775211688681424
1102	58-59	0.28215871200920617
1102	60-61	0.3101105937426354
1102	62-63	0.29265344846817953
1102	64-65	0.2782054334018156
1102	66-67	0.2677787462941197
1102	68-69	0.2502508634870537
1102	70-71	0.25376891175146454
1102	72-73	0.26517629144548494
1102	74-75	0.16568564810434339
1102	76-77	0.22373140145120374
1102	78-79	0.21030251587872328
1102	80-81	0.2558587166016508
1102	82-83	0.22385931049111463
1102	84-85	0.18707379634133048
1102	86-87	0.23967817940175706
1102	88-89	0.2064736126287201
1102	90-91	0.2600445000056766
1102	92-93	0.2528106153869203
1102	94-95	0.25277840474617363
1102	96-97	0.18085860768987772
1102	98-99	0.1873984695153652
1102	100	0.2201766774316809
1103	1	0.2062701230021986
1103	2	0.19933150561104895
1103	3	0.19094950719352966
1103	4	0.14168971112525952
1103	5	0.14546833849824736
1103	6	0.117863828290524
1103	7	0.11816060510992799
1103	8	0.08749802464824796
1103	9	0.1622967846246155
1103	10-11	0.19168950602352908
1103	12-13	0.17029072811659063
1103	14-15	0.2437740583262027
1103	16-17	0.21440107453344837
1103	18-19	0.2116393469816984
1103	20-21	0.2579952244701289
1103	22-23	0.24513344467793274
1103	24-25	0.23706740763290668
1103	26-27	0.2904803366067412
1103	28-29	0.2761838329044295
1103	30-31	0.24918946906477402
1103	32-33	0.2680361394667443
1103	34-35	0.32548581973576063
1103	36-37	0.3736893852233294
1103	38-39	0.40498095049136396
1103	40-41	0.39607896352061545
1103	42-43	0.4054961033316502
1103	44-45	0.39978643985967466
1103	46-47	0.44120208375687753
1103	48-49	0.4405277687942686
1103	50-51	0.43966900015603727
1103	52-53	0.44364670136653217
1103	54-55	0.43201959566948744
1103	56-57	0.4017367784184458
1103	58-59	0.3817752587137164
1103	60-61	0.35850948599988897
1103	62-63	0.38480740178425066
1103	64-65	0.4245535436498713
1103	66-67	0.3668550316478374
1103	68-69	0.36963347732955754
1103	70-71	0.3589939386980632
1103	72-73	0.38072095636548653
1103	74-75	0.3632169165756025
1103	76-77	0.33655567731572233
1103	78-79	0.32528632267805335
1103	80-81	0.35911498514610685
1103	82-83	0.32460578522101713
1103	84-85	0.2858871531581215
1103	86-87	0.2770946518434627
1103	88-89	0.2945869138443342
1103	90-91	0.2608690438974435
1103	92-93	0.24328124383778515
1103	94-95	0.3093519597658201
1103	96-97	0.2557869977356191
1103	98-99	0.22302041472748613
1103	100	0.19541350410619174
1104	1	0.15230853128138477
1104	2	0.13792259886354685
1104	3	0.16720219101181755
1104	4	0.08769160792547126
1104	5	0.03628509194117413
1104	6	0.07598391063064724
1104	7	0.09784132538066359
1104	8	0.0767441428171054
1104	9	0.13449422381599874
1104	10-11	0.14755476390750744
1104	12-13	0.15398642771887694
1104	14-15	0.23675349126251177
1104	16-17	0.20244908359907754
1104	18-19	0.19140876319588074
1104	20-21	0.2206700977707996
1104	22-23	0.21543015338306049
1104	24-25	0.20336161764385707
1104	26-27	0.20377271270192665
1104	28-29	0.19644844166693076
1104	30-31	0.2161325920090036
1104	32-33	0.20761527248274092
1104	34-35	0.19227542121521424
1104	36-37	0.23409632779443257
1104	38-39	0.220919125456426
1104	40-41	0.17368837924711755
1104	42-43	0.19042863730529547
1104	44-45	0.1803193566584227
1104	46-47	0.1927910870640801
1104	48-49	0.21670725319314954
1104	50-51	0.19779309298373704
1104	52-53	0.22424083424778019
1104	54-55	0.19858568465482307
1104	56-57	0.24711198038659887
1104	58-59	0.3106368449160968
1104	60-61	0.29008325266265444
1104	62-63	0.24998648770108645
1104	64-65	0.2505446955517385
1104	66-67	0.20451811411501097
1104	68-69	0.17364529639689152
1104	70-71	0.15083609360684136
1104	72-73	0.13001594473186628
1104	74-75	0.1398165717378319
1104	76-77	0.1387392273865018
1104	78-79	0.1209893258594974
1104	80-81	0.1694824163935138
1104	82-83	0.16431676702688236
1104	84-85	0.1530539971418179
1104	86-87	0.15623894533671745
1104	88-89	0.17334231837740077
1104	90-91	0.15236902400542363
1104	92-93	0.15683686833808252
1104	94-95	0.15380972259392678
1104	96-97	0.13229912801678978
1104	98-99	0.10965535255530767
1104	100	0.16919415546070837
1105	1	0.13863244128950925
1105	2	0.14055184096647366
1105	3	0.1752057371732647
1105	4	0.11633489446349188
1105	5	0.10789155565289832
1105	6	0.10840076278434196
1105	7	0.11781331245791904
1105	8	0.0902049510147549
1105	9	0.13630965984195598
1105	10-11	0.13899899219384082
1105	12-13	0.13900915353107735
1105	14-15	0.17605556345932172
1105	16-17	0.21065009311588057
1105	18-19	0.22269012802987476
1105	20-21	0.19209224287715898
1105	22-23	0.16341937176702004
1105	24-25	0.203579163256137
1105	26-27	0.25149071107781396
1105	28-29	0.2747325844247541
1105	30-31	0.2490385749149837
1105	32-33	0.21948885211948266
1105	34-35	0.23746692561724814
1105	36-37	0.28804961135297447
1105	38-39	0.27496690516210265
1105	40-41	0.2362299312623506
1105	42-43	0.23976804059206103
1105	44-45	0.22332744506125124
1105	46-47	0.2942851339528687
1105	48-49	0.24542451546304278
1105	50-51	0.24834371195541394
1105	52-53	0.18788501170774197
1105	54-55	0.17051485197873006
1105	56-57	0.23944435561372046
1105	58-59	0.24209106321242047
1105	60-61	0.2792835260734563
1105	62-63	0.21854541415475381
1105	64-65	0.22766852201766596
1105	66-67	0.25536967483498785
1105	68-69	0.3118866602961461
1105	70-71	0.2121873154843783
1105	72-73	0.2200329452751788
1105	74-75	0.1960501773135448
1105	76-77	0.19020245713920048
1105	78-79	0.16147720055789705
1105	80-81	0.18291416008841566
1105	82-83	0.1782147111879837
1105	84-85	0.21093018528115692
1105	86-87	0.18314456895286924
1105	88-89	0.11871967493896562
1105	90-91	0.11751133783194234
1105	92-93	0.13661973167357644
1105	94-95	0.16801364732416602
1105	96-97	0.09357739653189867
1105	98-99	0.08709282736120372
1105	100	0.15974103548127516
1106	1	0.12561492867349955
1106	2	0.1014598476481936
1106	3	0.13752562481779762
1106	4	0.0951925134406082
1106	5	0.10512906445054426
1106	6	0.08501403737962221
1106	7	0.07430368893139416
1106	8	0.04855860999811057
1106	9	0.11127791505442985
1106	10-11	0.14298460297460736
1106	12-13	0.10942753220433588
1106	14-15	0.13389660547083082
1106	16-17	0.1579168617253046
1106	18-19	0.16975559632303572
1106	20-21	0.20918828155623714
1106	22-23	0.13261554328688874
1106	24-25	0.14637695701242137
1106	26-27	0.1825623224798676
1106	28-29	0.185297152942411
1106	30-31	0.16703000669096468
1106	32-33	0.18599722686792575
1106	34-35	0.14440789489741235
1106	36-37	0.17864346318427948
1106	38-39	0.22017398486146078
1106	40-41	0.17793718356211485
1106	42-43	0.1839263153677564
1106	44-45	0.1599157717442452
1106	46-47	0.15775646051241665
1106	48-49	0.11427276272912223
1106	50-51	0.16482632452136414
1106	52-53	0.18985366548405835
1106	54-55	0.1738817418162597
1106	56-57	0.14215273724682476
1106	58-59	0.14810972285728496
1106	60-61	0.1624569983940063
1106	62-63	0.11332753189601164
1106	64-65	0.14784786606978173
1106	66-67	0.14816346636329314
1106	68-69	0.03759328164017717
1106	70-71	0.03272839458734467
1106	72-73	0.059041147561465834
1106	74-75	0.07031702652052019
1106	76-77	-0.0010283503113228676
1106	78-79	-2.5475357588078396E-4
1106	80-81	0.02788377426119837
1106	82-83	0.05379274281346369
1106	84-85	0.0927063892640021
1106	86-87	0.09067969282355648
1106	88-89	0.06948316040811875
1106	90-91	0.07930503272613976
1106	92-93	0.0721913818536919
1106	94-95	0.0771252619280034
1106	96-97	0.041522580800972264
1106	98-99	0.07771172940698534
1106	100	0.21518633967893663
1107	1	0.1159672130251721
1107	2	0.1250004146647683
1107	3	0.15680567263657963
1107	4	0.11236055599867001
1107	5	0.07396549664285601
1107	6	0.08233341219290935
1107	7	0.08697612674951216
1107	8	0.07830641033682184
1107	9	0.07876590957704366
1107	10-11	0.08405974828482954
1107	12-13	0.06737357248900366
1107	14-15	0.14517113744611976
1107	16-17	0.15136665538899052
1107	18-19	0.08967353034362446
1107	20-21	0.10430348565950709
1107	22-23	0.14931461998029505
1107	24-25	0.13867816280082934
1107	26-27	0.1394463475776817
1107	28-29	0.20322942663939614
1107	30-31	0.1685875838256976
1107	32-33	0.11935273714574635
1107	34-35	0.1314325406914847
1107	36-37	0.12297633077017878
1107	38-39	0.17503123674939047
1107	40-41	0.15435345884836948
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2216	38-39	-0.42729768524526435
2216	40-41	-0.4381643828172841
2216	42-43	-0.44388075568411267
2216	44-45	-0.42048004197173583
2216	46-47	-0.4546393803400832
2216	48-49	-0.4664079944205213
2216	50-51	-0.4688940957496399
2216	52-53	-0.4347087706883386
2216	54-55	-0.4835305287363383
2216	56-57	-0.4924031815587213
2216	58-59	-0.49933074825986523
2216	60-61	-0.48071018174185554
2216	62-63	-0.45337529744809757
2216	64-65	-0.43287212495995675
2216	66-67	-0.3855822332942651
2216	68-69	-0.4389095465712458
2216	70-71	-0.42277056941208
2216	72-73	-0.3977729068809026
2216	74-75	-0.4153893135003983
2216	76-77	-0.4073912671643001
2216	78-79	-0.44321318465453885
2216	80-81	-0.3794005334055086
2216	82-83	-0.3277973257976967
2216	84-85	-0.32048731521616247
2216	86-87	-0.32087089155513127
2216	88-89	-0.3010342287059373
2216	90-91	-0.33900369506411465
2216	92-93	-0.36587555196624777
2216	94-95	-0.31265733314631916
2216	96-97	-0.3446580619090227
2216	98-99	-0.32308666054103696
2216	100	-0.43323847056606724
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	24.0
8	90.0
9	143.0
10	214.0
11	276.0
12	228.0
13	202.0
14	369.0
15	672.0
16	1404.0
17	2608.0
18	4808.0
19	7806.0
20	11145.0
21	17019.0
22	24707.0
23	29880.0
24	28779.0
25	24877.0
26	21714.0
27	23053.0
28	27366.0
29	32827.0
30	39732.0
31	51187.0
32	66773.0
33	88980.0
34	129640.0
35	205805.0
36	374557.0
37	947916.0
38	1478728.0
39	552746.0
40	1835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.696599503512125	19.764586326406594	13.586001719436469	23.95281245064481
2	15.242592834966494	21.123481716226568	40.01982539066104	23.614100058145898
3	17.546380009389985	26.464743141585068	31.192511072294526	24.796365776730422
4	12.035487397900484	18.150096114680178	39.50275242906927	30.31166405835007
5	12.592190842848897	38.33516265383807	35.699821134871364	13.37282536844167
6	29.21906053243848	39.10995678585474	18.288342519207674	13.382640162499099
7	25.488255429228868	33.66810302731467	23.22402223857879	17.61961930487767
8	25.261367615426945	34.984981336839674	21.177482247963965	18.57616879976942
9	26.278468167818374	14.711191464730557	20.886565621196205	38.12377474625486
10-11	24.025597233040582	26.393579491723656	29.296933620129057	20.2838896551067
12-13	25.186333850232607	24.41000831326478	28.88343802330096	21.520219813201656
14-15	22.424503797364935	25.314888836246137	27.098942297465634	25.1616650689233
16-17	21.315615836650302	28.172237030473234	27.854784653093894	22.65736247978257
18-19	21.212879128941356	27.082365635964756	30.29133348101884	21.41342175407505
20-21	22.42560342630374	26.39162623180431	29.942557484201227	21.240212857690725
22-23	22.22519050330031	26.34183640655631	29.628509631761112	21.804463458382266
24-25	21.941409348492535	26.468875279590105	29.991698645342783	21.598016726574578
26-27	21.749477741174005	26.689010054524537	29.69861437646941	21.862897827832047
28-29	21.711641871180348	26.74357350612222	29.82612768914198	21.71865693355545
30-31	22.041866995195928	26.444878449890712	29.780360372206122	21.73289418270724
32-33	21.9744306897468	26.654507025360996	29.48819812481561	21.882864160076586
34-35	22.16150091572267	26.662244772901133	29.61729498013423	21.558959331241965
36-37	22.162996650368715	26.451653782186785	29.498979770247026	21.886369797197478
38-39	22.041102365974726	26.62619895597499	29.593829750008304	21.738868928041978
40-41	22.199603244174433	26.380260540136753	29.61465662136307	21.805479594325742
42-43	22.28439341166427	26.36453850635742	29.255248701432034	22.095819380546278
44-45	22.18825041584397	26.848966279657244	29.038069464463472	21.924713840035313
46-47	22.45049631422241	26.671814237714642	28.49148417622129	22.386205271841657
48-49	22.605016855292217	26.8198403654235	28.3674496580348	22.207693121249484
50-51	22.497809714055133	27.018409835729745	28.03744662443468	22.44633382578044
52-53	22.342886265114252	27.12757816665795	28.05647029566356	22.473065272564234
54-55	22.45819063787579	27.078081382205948	27.913208606962815	22.550519372955446
56-57	22.679316137722893	27.024410636560752	27.81601296310743	22.480260262608926
58-59	22.284851289215872	27.075313884774825	28.18433074340802	22.455504082601287
60-61	22.49726121603199	27.230277718292218	27.9478760399816	22.324585025694187
62-63	22.386199748765414	27.0172818761771	27.9048448113698	22.69167356368768
64-65	22.16914303165103	27.056955938065048	27.990525308899965	22.783375721383955
66-67	22.433759019326192	27.01774445976946	27.81700742985233	22.73148909105202
68-69	22.30227840725763	26.896313430743472	28.01706164275959	22.78434651923931
70-71	22.370197414816467	26.707735052930076	27.906056602387043	23.01601092986641
72-73	22.472078869986298	26.744397669689768	27.902180044479426	22.88134341584451
74-75	22.446752315563362	27.01806416342253	27.70343399550138	22.831749525512734
76-77	22.388280669173763	26.883650198084634	27.667210661958343	23.06085847078326
78-79	22.290160743854955	26.89420976487684	27.669457667100634	23.14617182416757
80-81	22.32695454942278	27.033227694513446	27.59925628428701	23.040561471776762
82-83	22.583697614887292	26.679725738062437	27.976680422481248	22.759896224569022
84-85	22.61227231556124	26.941735055617322	27.467483860287047	22.978508768534393
86-87	22.129665999620304	27.117399762607402	27.68820437963855	23.064729858133745
88-89	22.51431600009528	26.716216860008096	27.591219837545555	23.178247302351064
90-91	22.417873757476578	26.66309363022884	27.708468810964938	23.210563801329645
92-93	22.507687983402054	26.663200821321976	27.686911491123382	23.14219970415258
94-95	22.14256202702669	26.656367586410024	27.846843918673393	23.35422646788989
96-97	22.20865521559432	26.621003870090536	27.80537496429927	23.364965950015876
98-99	22.038434676390466	26.808980001353	27.645528293383364	23.50705702887317
100	22.315124458055955	26.525429118685416	27.549992484661445	23.609453938597184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	406.0
1	259.5
2	276.5
3	454.0
4	316.0
5	231.5
6	519.0
7	1332.5
8	2580.0
9	3201.5
10	2426.0
11	1662.5
12	1781.5
13	2060.5
14	2389.0
15	2905.5
16	3529.0
17	4143.5
18	4842.5
19	5613.5
20	6551.5
21	7740.0
22	9187.0
23	11449.0
24	14350.0
25	17650.0
26	23031.5
27	29510.0
28	35374.5
29	42690.0
30	51653.5
31	60574.0
32	70683.5
33	80493.5
34	88725.5
35	96789.5
36	106797.5
37	118887.0
38	124828.5
39	126470.0
40	128502.5
41	129747.5
42	131488.5
43	137038.5
44	144560.0
45	154725.0
46	163684.5
47	167958.5
48	169844.5
49	166365.5
50	161976.0
51	159549.0
52	154441.0
53	148130.5
54	141505.5
55	129641.0
56	117762.0
57	103271.0
58	88252.5
59	78279.5
60	64282.5
61	49817.0
62	38965.5
63	29895.0
64	22203.0
65	15194.0
66	10547.0
67	7310.0
68	5283.5
69	3875.5
70	2742.0
71	1960.0
72	1412.5
73	1132.5
74	858.5
75	638.0
76	440.0
77	274.5
78	166.5
79	77.5
80	50.5
81	24.5
82	16.0
83	13.0
84	8.0
85	8.0
86	9.0
87	9.0
88	5.5
89	2.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005311914170900715
2	0.08310882754382329
3	4.5258461545790845E-4
4	0.0
5	0.039351041301919194
6	0.004263823482471874
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.5730364378255926E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.764048583767457E-4
24-25	0.0
26-27	0.0
28-29	1.1910121459418643E-5
30-31	1.429214575130237E-4
32-33	0.0016555068828591915
34-35	0.0014173044536708184
36-37	8.575287450781423E-4
38-39	7.265174090245372E-4
40-41	0.0014768550609679117
42-43	5.716858300520948E-4
44-45	5.00225101295583E-4
46-47	3.2157327940430337E-4
48-49	5.240453442144203E-4
50-51	6.66966801727444E-4
52-53	0.0010600008098882593
54-55	0.001595956275562098
56-57	0.0010123603240505847
58-59	0.0026797773283691946
60-61	0.0018817991905881457
62-63	9.885400811317474E-4
64-65	0.004513936033119666
66-67	0.004287643725390712
68-69	0.003704047773879198
70-71	0.0017031473686968661
72-73	0.005097531984631179
74-75	0.003751688259716873
76-77	0.0037397781382574537
78-79	7.97978137781049E-4
80-81	1.1910121459418643E-5
82-83	1.5483157897244236E-4
84-85	5.955060729709321E-5
86-87	2.3820242918837286E-5
88-89	2.3820242918837285E-4
90-91	0.0
92-93	0.0
94-95	1.1910121459418643E-5
96-97	4.049441296202339E-4
98-99	6.193263158897694E-4
100	6.907870446462812E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4198110.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.72732533249058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.85516051832103	35.77821124137882
2	16.55047227438051	17.45324272035253
3	6.705116305308851	10.606285464666211
4	3.2837827525192687	6.925803260532196
5	1.8647503300511328	4.916164865823762
6	1.0683353503963677	3.3798279350729756
7	0.6961332201462407	2.5693669941383583
8	0.4408263656579381	1.8594876157748443
9	0.317555689010539	1.5069475913077703
>10	1.1592682050074006	9.65307956093479
>50	0.03853536940810889	1.408947189224714
>100	0.018037892887571315	1.7541159417552157
>500	0.0011686885990403355	0.43710413805501047
>1k	6.233005861529204E-4	0.5178698086756645
>5k	7.791257326911505E-5	0.2545392142277025
>10k+	1.558251465382301E-4	0.9790064580794671
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12430	0.2960856194811475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11401	0.2715745895176639	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6196	0.14759022512511583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.764048583767457E-5	0.0
3	0.0	0.0	0.0	4.764048583767457E-5	0.0
4	0.0	0.0	0.0	7.146072875651185E-5	0.0
5	0.0	0.0	0.0	9.528097167534915E-5	0.0
6	0.0	0.0	0.0	9.528097167534915E-5	0.0
7	0.0	0.0	0.0	9.528097167534915E-5	2.3820242918837286E-5
8	0.0	0.0	0.0	9.528097167534915E-5	2.3820242918837286E-5
9	0.0	0.0	0.0	9.528097167534915E-5	2.3820242918837286E-5
10-11	0.0	0.0	0.0	2.3820242918837285E-4	2.3820242918837286E-5
12-13	0.0	0.0	0.0	2.3820242918837285E-4	2.3820242918837286E-5
14-15	0.0	0.0	0.0	3.096631579448847E-4	3.573036437825593E-5
16-17	0.0	0.0	0.0	3.096631579448847E-4	4.764048583767457E-5
18-19	0.0	0.0	0.0	3.33483400863722E-4	4.764048583767457E-5
20-21	0.0	0.0	0.0	3.33483400863722E-4	5.955060729709321E-5
22-23	0.0	0.0	0.0	3.33483400863722E-4	7.146072875651185E-5
24-25	0.0	0.0	0.0	6.431465588086067E-4	7.146072875651185E-5
26-27	0.0	0.0	0.0	0.0012505627532389575	7.146072875651185E-5
28-29	0.0	0.0	0.0	0.0034301149803125696	7.146072875651185E-5
30-31	0.0	0.0	0.0	0.010528547370126082	7.146072875651185E-5
32-33	0.0	0.0	0.0	0.027762493121904857	7.146072875651185E-5
34-35	0.0	0.0	0.0	0.05660880729661681	7.146072875651185E-5
36-37	0.0	0.0	0.0	0.10239131418662208	7.146072875651185E-5
38-39	0.0	0.0	0.0	0.16553877816445972	8.337085021593049E-5
40-41	0.0	0.0	0.0	0.22943657979424076	9.528097167534915E-5
42-43	0.0	0.0	0.0	0.29476359599915203	9.528097167534915E-5
44-45	0.0	0.0	0.0	0.3743112972266091	9.528097167534915E-5
46-47	0.0	0.0	0.0	0.4720695741655173	9.528097167534915E-5
48-49	0.0	0.0	0.0	0.5738772924006279	9.528097167534915E-5
50-51	0.0	0.0	0.0	0.6758041118503326	9.528097167534915E-5
52-53	0.0	0.0	0.0	0.7743484568055625	9.528097167534915E-5
54-55	0.0	0.0	0.0	0.8795743798995262	9.528097167534915E-5
56-57	0.0	0.0	0.0	1.0051904309320145	9.528097167534915E-5
58-59	0.0	0.0	0.0	1.1467184042342864	9.528097167534915E-5
60-61	0.0	0.0	0.0	1.2893659289537434	9.528097167534915E-5
62-63	0.0	0.0	0.0	1.4263442358585174	9.528097167534915E-5
64-65	0.0	0.0	0.0	1.5681699621972744	9.528097167534915E-5
66-67	0.0	0.0	0.0	1.727837050482241	9.528097167534915E-5
68-69	0.0	0.0	0.0	1.907251120146923	9.528097167534915E-5
70-71	0.0	0.0	0.0	2.0904049679498633	9.528097167534915E-5
72-73	0.0	0.0	0.0	2.277393874862736	1.1910121459418643E-4
74-75	0.0	0.0	0.0	2.4692778416954297	1.5483157897244236E-4
76-77	0.0	0.0	0.0	2.672643165615003	1.66741700431861E-4
78-79	0.0	0.0	0.0	2.891277265245551	1.66741700431861E-4
80-81	0.0	0.0	0.0	3.129991829656679	1.66741700431861E-4
82-83	0.0	0.0	0.0	3.3757810062147016	1.66741700431861E-4
84-85	0.0	0.0	0.0	3.619664563339217	1.66741700431861E-4
86-87	0.0	0.0	0.0	3.8738265552832107	1.66741700431861E-4
88	0.0	0.0	0.0	4.077215699445703	1.66741700431861E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8110	0.0	61.32535	1
GTATCAA	14570	0.0	42.240993	1
ATCAACG	18060	0.0	33.528687	3
CAACGCA	18125	0.0	33.434383	5
TATCAAC	18965	0.0	32.25193	2
TCAACGC	19195	0.0	31.71758	4
AACGCAG	19460	0.0	31.372578	6
ACGCAGA	21050	0.0	28.733915	7
CGCAGAG	21410	0.0	28.228815	8
GCAGAGT	24025	0.0	24.980198	9
GTACTAG	1900	0.0	24.517012	1
GAGTACT	16465	0.0	24.019321	12-13
TACATGG	12970	0.0	23.942484	2
GTACATG	13680	0.0	23.698402	1
GTGGTAT	2540	0.0	23.526424	1
CAGAGTA	23270	0.0	23.094479	10-11
ACATGGG	13360	0.0	22.556408	3
AGAGTAC	22110	0.0	21.787304	10-11
GTACTTT	18130	0.0	21.657934	14-15
TGGTATC	2785	0.0	21.286808	2
>>END_MODULE