FastQCFastQC Report
Mon 16 Jan 2017
ERR1146555_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146555_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699660
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39700.037.5436481
CAACGCA40750.034.7114875
ATCAACG41350.034.321463
GGTATCA24900.033.8003651
TATCAAC49350.030.0908372
AACGCAG48600.029.7811036
TCAACGC48950.028.9926974
ACGCAGA51900.027.2521867
CGCAGAG52650.026.8644738
GTACATG87500.025.1481191
TACATGG86950.024.4829062
ACATGGG85550.023.894813
GTACTAG12600.022.7628691
TGATCGC7700.022.5798599
GTGATCG7700.021.9695938
CATGGGG65200.021.6226044
GCAGAGT64150.021.0230379
TAGTACT20000.020.9119424
CTAACGC4850.020.3475423
GTATAAG17600.020.3033941
Produced by FastQC (version 0.11.5)