##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146555_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2699660
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310894334842164	31.0	31.0	34.0	28.0	34.0
2	31.391814154374995	31.0	31.0	34.0	28.0	34.0
3	31.600307075705828	33.0	31.0	34.0	28.0	34.0
4	35.10573998207182	37.0	35.0	37.0	32.0	37.0
5	35.0868287117637	37.0	35.0	37.0	32.0	37.0
6	35.17142825392827	37.0	35.0	37.0	32.0	37.0
7	35.024396775890295	37.0	35.0	37.0	32.0	37.0
8	34.97449715890149	37.0	35.0	37.0	32.0	37.0
9	36.58613899528089	39.0	37.0	39.0	32.0	39.0
10-11	36.6013377610514	39.0	37.0	39.0	32.0	39.0
12-13	36.57342591289273	39.0	37.0	39.0	32.0	39.0
14-15	37.95827307883215	40.0	38.0	41.0	32.0	41.0
16-17	37.961364764451815	40.0	38.0	41.0	32.0	41.0
18-19	37.98320677418638	40.0	38.0	41.0	32.0	41.0
20-21	38.090900706014835	40.0	38.0	41.0	32.0	41.0
22-23	38.09790825511361	40.0	38.0	41.0	32.0	41.0
24-25	38.15450667861879	40.0	38.0	41.0	32.0	41.0
26-27	38.190105050265586	40.0	38.0	41.0	32.5	41.0
28-29	38.16022388004416	40.0	38.0	41.0	32.0	41.0
30-31	38.15627856841232	40.0	38.0	41.0	32.5	41.0
32-33	38.173887822911034	40.0	38.0	41.0	33.0	41.0
34-35	38.11571457146455	40.0	38.0	41.0	32.5	41.0
36-37	38.022430602372154	40.0	38.0	41.0	32.0	41.0
38-39	37.98283895009001	40.0	38.0	41.0	32.0	41.0
40-41	37.92609032248505	40.0	38.0	41.0	32.0	41.0
42-43	37.793637346925166	40.0	38.0	41.0	31.5	41.0
44-45	37.64022932517428	40.0	37.0	41.0	31.0	41.0
46-47	37.537124674959074	40.0	37.0	41.0	31.0	41.0
48-49	37.41695972826207	40.0	37.0	41.0	31.0	41.0
50-51	37.29063011638503	40.0	36.0	41.0	31.0	41.0
52-53	37.168590489172715	40.0	36.0	41.0	30.0	41.0
54-55	36.99238330011927	39.5	35.5	41.0	30.0	41.0
56-57	36.86538415948675	39.0	35.0	41.0	30.0	41.0
58-59	36.64179452227317	39.0	35.0	41.0	30.0	41.0
60-61	36.464723891156666	39.0	35.0	40.5	30.0	41.0
62-63	36.23087333219739	38.0	35.0	40.0	30.0	41.0
64-65	35.97866101657246	37.5	35.0	40.0	30.0	41.0
66-67	35.71816062022625	37.0	35.0	40.0	29.0	41.0
68-69	35.43074961291422	36.5	34.5	39.5	29.0	41.0
70-71	35.17334479156635	36.0	34.0	39.0	29.0	41.0
72-73	34.86309590837365	35.5	34.0	39.0	29.0	40.5
74-75	34.55741537823282	35.0	34.0	38.5	29.0	40.0
76-77	34.258723876339985	35.0	34.0	37.0	29.0	39.5
78-79	33.98882340739205	35.0	34.0	37.0	28.0	39.0
80-81	33.719304653178554	35.0	34.0	36.0	28.0	39.0
82-83	33.47381207263137	35.0	34.0	36.0	28.0	37.5
84-85	33.2575226139588	35.0	33.0	36.0	28.0	37.0
86-87	33.07045702051369	35.0	33.0	35.0	27.0	37.0
88-89	32.92277008956683	35.0	33.0	35.0	27.0	36.0
90-91	32.766690805508844	35.0	33.0	35.0	27.0	36.0
92-93	32.67781813265374	35.0	33.0	35.0	27.0	36.0
94-95	32.58386241230377	35.0	33.0	35.0	27.0	36.0
96-97	32.51311942985413	35.0	33.0	35.0	27.0	35.0
98-99	32.442725750650084	35.0	33.0	35.0	26.5	35.0
100	31.512759014098073	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28389079211852675
1101	2	0.28781303255658486
1101	3	0.39910267850190806
1101	4	0.422921507073589
1101	5	0.4357556468123249
1101	6	0.3792453897395447
1101	7	0.2938397599080673
1101	8	0.4026460673072805
1101	9	0.46500007308321756
1101	10-11	0.49936757044446267
1101	12-13	0.5415584446788628
1101	14-15	0.6217487027501889
1101	16-17	0.5820879497199059
1101	18-19	0.5048894840445044
1101	20-21	0.5356427850082071
1101	22-23	0.513952545353348
1101	24-25	0.4517778244088646
1101	26-27	0.5222688294912317
1101	28-29	0.5135289435777395
1101	30-31	0.5394408303252902
1101	32-33	0.5048913402501611
1101	34-35	0.5754637640442226
1101	36-37	0.5818642431206058
1101	38-39	0.5208302200645889
1101	40-41	0.5114871874925413
1101	42-43	0.5259091075415583
1101	44-45	0.42629560836432745
1101	46-47	0.4477195153625999
1101	48-49	0.42151154673917546
1101	50-51	0.42048096782254873
1101	52-53	0.4225558816386439
1101	54-55	0.5113603749640205
1101	56-57	0.49817917867402883
1101	58-59	0.544895080657632
1101	60-61	0.5024641405097796
1101	62-63	0.44651560493241504
1101	64-65	0.38115105782217285
1101	66-67	0.36613731585702425
1101	68-69	0.3937662211365378
1101	70-71	0.3641322618421867
1101	72-73	0.32156217019855404
1101	74-75	0.3571786508604049
1101	76-77	0.3903572313058632
1101	78-79	0.3962938117240995
1101	80-81	0.34023203468997565
1101	82-83	0.2948094469092055
1101	84-85	0.3166001086377861
1101	86-87	0.38821144324268886
1101	88-89	0.3447198957629567
1101	90-91	0.3586873426670607
1101	92-93	0.3882020285959271
1101	94-95	0.35068380218957884
1101	96-97	0.26849539359647423
1101	98-99	0.21293487673163014
1101	100	0.29322269747948937
1102	1	0.27410170472325035
1102	2	0.24309280564845537
1102	3	0.3537853948948957
1102	4	0.26936058805814866
1102	5	0.2829264877420954
1102	6	0.3117453037421143
1102	7	0.2969898457491382
1102	8	0.27287227181854945
1102	9	0.33848300024808964
1102	10-11	0.42699494827338924
1102	12-13	0.44556725394281926
1102	14-15	0.48188166259407694
1102	16-17	0.4530105379610845
1102	18-19	0.41206417140476503
1102	20-21	0.3795639003479607
1102	22-23	0.383422402472533
1102	24-25	0.42712075870370825
1102	26-27	0.468535954706617
1102	28-29	0.472503127149416
1102	30-31	0.41857038779815525
1102	32-33	0.48597413388447563
1102	34-35	0.4442902040320931
1102	36-37	0.47888912295874064
1102	38-39	0.4089297394171396
1102	40-41	0.5431150875604018
1102	42-43	0.42444902754831304
1102	44-45	0.3892888427120127
1102	46-47	0.37789695151342073
1102	48-49	0.3532336530820288
1102	50-51	0.31615662617195994
1102	52-53	0.2665526356265815
1102	54-55	0.3353569022314673
1102	56-57	0.31622511381007
1102	58-59	0.3306409285453782
1102	60-61	0.2651379725865084
1102	62-63	0.3226863817550054
1102	64-65	0.2782403575529244
1102	66-67	0.32881110884400755
1102	68-69	0.3095504629675574
1102	70-71	0.22335869151036292
1102	72-73	0.15999461687233207
1102	74-75	0.09773308410606774
1102	76-77	0.15544379944553555
1102	78-79	0.13770997830268783
1102	80-81	0.1442929505782402
1102	82-83	0.1217554905583782
1102	84-85	0.08727033351324565
1102	86-87	0.09398890691269202
1102	88-89	0.162022500390556
1102	90-91	0.21326439949103104
1102	92-93	0.20843413565803814
1102	94-95	0.12290901694861134
1102	96-97	0.1112530418951323
1102	98-99	0.04118499321905489
1102	100	0.025426154888872787
1103	1	0.21724113599126937
1103	2	0.1736799556818731
1103	3	0.2444653725547674
1103	4	0.16842917413510605
1103	5	0.17591742932203402
1103	6	0.14489741115043842
1103	7	0.1279860680083118
1103	8	0.2632238935173419
1103	9	0.262248096307502
1103	10-11	0.3571120770648548
1103	12-13	0.2671197516758568
1103	14-15	0.34961276683023357
1103	16-17	0.2786541109383194
1103	18-19	0.3506579983754463
1103	20-21	0.3369409668882781
1103	22-23	0.23644628162247727
1103	24-25	0.23136918752121005
1103	26-27	0.22346564762398202
1103	28-29	0.30060701659088096
1103	30-31	0.30872327805227684
1103	32-33	0.22315487713301962
1103	34-35	0.26500169162736853
1103	36-37	0.27066712420753447
1103	38-39	0.24685995161697605
1103	40-41	0.2419175217558589
1103	42-43	0.19619441283708738
1103	44-45	0.22151592917646923
1103	46-47	0.2046748272558716
1103	48-49	0.09711568967340156
1103	50-51	0.06595636511877245
1103	52-53	0.13534595492411938
1103	54-55	0.165182581769848
1103	56-57	0.06146709003289885
1103	58-59	0.14359835993262493
1103	60-61	0.10602778115796951
1103	62-63	0.08919215190897489
1103	64-65	0.09871475582846045
1103	66-67	0.05248635020839032
1103	68-69	0.12645306847917226
1103	70-71	0.13072575676424947
1103	72-73	0.09089470911948894
1103	74-75	0.09530398734382572
1103	76-77	0.0989793208773122
1103	78-79	0.12371292299518188
1103	80-81	0.08370966770890931
1103	82-83	0.060218084962677665
1103	84-85	0.12981490395210926
1103	86-87	0.06083389717026222
1103	88-89	0.0038397223704791372
1103	90-91	0.06679775593844539
1103	92-93	0.09361964187217353
1103	94-95	0.028515973451256116
1103	96-97	0.018434946077320546
1103	98-99	-6.85451293016115E-4
1103	100	-0.05425319210164403
1104	1	0.025807713725207293
1104	2	0.08781802402540606
1104	3	0.15807549364055618
1104	4	0.12201199757527803
1104	5	0.15482025743950345
1104	6	0.11309507269585595
1104	7	0.15203740439530122
1104	8	0.07686775284756919
1104	9	0.1810049578891082
1104	10-11	0.16514921700672858
1104	12-13	0.2437825302837382
1104	14-15	0.30175700106710224
1104	16-17	0.28142236854915126
1104	18-19	0.19369951713452593
1104	20-21	0.23072966441988996
1104	22-23	0.2444144545411504
1104	24-25	0.25561272830935167
1104	26-27	0.2818584104240003
1104	28-29	0.23586029228227545
1104	30-31	0.2879225361835296
1104	32-33	0.25485429419249783
1104	34-35	0.2531784007317768
1104	36-37	0.2582524638186925
1104	38-39	0.24159527259273972
1104	40-41	0.28349728095557225
1104	42-43	0.2239137678798997
1104	44-45	0.1632333541263904
1104	46-47	0.2060556582540869
1104	48-49	0.2418540625182004
1104	50-51	0.2345122095150458
1104	52-53	0.2632908501682465
1104	54-55	0.2056947830229845
1104	56-57	0.17913199362104137
1104	58-59	0.2666660032868151
1104	60-61	0.24326444380093193
1104	62-63	0.23871812100408363
1104	64-65	0.21048735365166493
1104	66-67	0.19870009119093623
1104	68-69	0.1647664334967942
1104	70-71	0.12150747270760576
1104	72-73	0.20044544195187086
1104	74-75	0.22091560015635991
1104	76-77	0.20924215648322786
1104	78-79	0.14629988393505755
1104	80-81	0.15849588596024944
1104	82-83	0.1771161086408526
1104	84-85	0.1520948148211403
1104	86-87	0.13156623469252082
1104	88-89	0.1605848708862041
1104	90-91	0.23995942592416242
1104	92-93	0.22297044635453034
1104	94-95	0.1852888459054114
1104	96-97	0.1138791502724743
1104	98-99	0.07535941140529445
1104	100	0.19785570901906624
1105	1	0.180850631130685
1105	2	0.1695384789233536
1105	3	0.1392565990833745
1105	4	0.16060713197249754
1105	5	0.1576602956131481
1105	6	0.045048733355621096
1105	7	0.06553064226658023
1105	8	0.10174014639940765
1105	9	0.1495865945771655
1105	10-11	0.2417899362138627
1105	12-13	0.19670996822012654
1105	14-15	0.23627236204698931
1105	16-17	0.19148853666689547
1105	18-19	0.24815452966112872
1105	20-21	0.2415682829586956
1105	22-23	0.21026001056712573
1105	24-25	0.23306743203742997
1105	26-27	0.2667762923735353
1105	28-29	0.1626665191136496
1105	30-31	0.2024054663321948
1105	32-33	0.19894474980306143
1105	34-35	0.1904524782835253
1105	36-37	0.19329732344112927
1105	38-39	0.18986097066603236
1105	40-41	0.16795919603737275
1105	42-43	0.155049356979454
1105	44-45	0.04647970688048986
1105	46-47	0.16317255596074176
1105	48-49	0.11822129941417359
1105	50-51	0.11609485783264972
1105	52-53	0.08383894658604163
1105	54-55	0.08992555332858387
1105	56-57	0.06816346312474053
1105	58-59	0.09673599296093016
1105	60-61	0.11846463886099201
1105	62-63	0.10178588760829399
1105	64-65	0.08378627201847877
1105	66-67	0.05818121885048555
1105	68-69	0.0492829472313403
1105	70-71	-0.0075166540981967955
1105	72-73	-1.1118759175587911E-5
1105	74-75	0.008913153521916684
1105	76-77	0.035706609022568614
1105	78-79	-0.0205809505754857
1105	80-81	0.08554611252893807
1105	82-83	0.06199462227200314
1105	84-85	0.028974992610841355
1105	86-87	0.07915715210358343
1105	88-89	0.060406228845174326
1105	90-91	0.10843102693970508
1105	92-93	0.07162682346319826
1105	94-95	0.03364714860341422
1105	96-97	0.0044004329181390744
1105	98-99	0.020705112049576258
1105	100	0.015138785043586012
1106	1	0.23005741724143647
1106	2	0.25026923779283905
1106	3	0.2979999114248457
1106	4	0.3784880210487884
1106	5	0.34139576691494256
1106	6	0.30376373986921834
1106	7	0.313694147139735
1106	8	0.33839977239808405
1106	9	0.32757311970167535
1106	10-11	0.3801214931124832
1106	12-13	0.38255464665941474
1106	14-15	0.4118116368711995
1106	16-17	0.3478416983953281
1106	18-19	0.3411905151529311
1106	20-21	0.37871223143298494
1106	22-23	0.3715934037656581
1106	24-25	0.41933585197358525
1106	26-27	0.3567228756974359
1106	28-29	0.38550613373794107
1106	30-31	0.42439004974198014
1106	32-33	0.39272914789101776
1106	34-35	0.3702647618415469
1106	36-37	0.3684799448340783
1106	38-39	0.40654234334902384
1106	40-41	0.3512307120454494
1106	42-43	0.32051881726865616
1106	44-45	0.29593877274913183
1106	46-47	0.2552947512436248
1106	48-49	0.2125976471945421
1106	50-51	0.276654200512759
1106	52-53	0.25631305814094674
1106	54-55	0.29607640972628957
1106	56-57	0.21765418597099284
1106	58-59	0.2525703121179248
1106	60-61	0.27339792169933474
1106	62-63	0.23090894052522515
1106	64-65	0.30181331026583536
1106	66-67	0.2603324943045777
1106	68-69	0.20936427931010826
1106	70-71	0.2486574651350537
1106	72-73	0.27523846055765944
1106	74-75	0.23717001557037065
1106	76-77	0.21477200914984707
1106	78-79	0.2381661684118015
1106	80-81	0.22899324830451206
1106	82-83	0.22487442466479735
1106	84-85	0.2939275949900946
1106	86-87	0.23898132410931083
1106	88-89	0.24379018731433888
1106	90-91	0.2310533699334627
1106	92-93	0.16276488692812308
1106	94-95	0.21483343608911554
1106	96-97	0.24410602627986577
1106	98-99	0.21465394422269668
1106	100	0.12887186173741227
1107	1	0.24400662475234114
1107	2	0.25909262524175247
1107	3	0.27140957954115663
1107	4	0.35061548581828816
1107	5	0.28610474647913975
1107	6	0.1753576943000752
1107	7	0.14894458057946736
1107	8	0.16233468802808915
1107	9	0.2873898483738273
1107	10-11	0.3605452394610893
1107	12-13	0.3385171572560992
1107	14-15	0.4130157019565672
1107	16-17	0.3872592708858775
1107	18-19	0.24599500798447593
1107	20-21	0.32821106147933676
1107	22-23	0.3224536043758235
1107	24-25	0.3137394437643337
1107	26-27	0.2989158094792259
1107	28-29	0.30210258198290063
1107	30-31	0.27245272598180037
1107	32-33	0.2819343626627244
1107	34-35	0.34112315024270856
1107	36-37	0.2640012006238308
1107	38-39	0.26731583231531175
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2216	64-65	-0.41914013638997005
2216	66-67	-0.38350531988727
2216	68-69	-0.40012418625191515
2216	70-71	-0.4361728692359961
2216	72-73	-0.3848078038616123
2216	74-75	-0.37996886540179986
2216	76-77	-0.34575215839746676
2216	78-79	-0.2983239850210637
2216	80-81	-0.3111630429867418
2216	82-83	-0.27451893107073033
2216	84-85	-0.24929386470572013
2216	86-87	-0.2882554550531182
2216	88-89	-0.3169001611078315
2216	90-91	-0.28509195678289956
2216	92-93	-0.25066678737081816
2216	94-95	-0.22327182450239036
2216	96-97	-0.2235421584392796
2216	98-99	-0.14554989777824545
2216	100	-0.24943049273451834
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	81.0
1	32.0
2	37.0
3	37.0
4	33.0
5	35.0
6	75.0
7	387.0
8	1075.0
9	1497.0
10	1894.0
11	2714.0
12	3859.0
13	5071.0
14	5939.0
15	6441.0
16	7061.0
17	7505.0
18	8421.0
19	9162.0
20	10444.0
21	11660.0
22	13054.0
23	15127.0
24	18586.0
25	23455.0
26	26273.0
27	25169.0
28	26525.0
29	33393.0
30	37965.0
31	41501.0
32	52706.0
33	70611.0
34	104519.0
35	161264.0
36	307885.0
37	649411.0
38	774772.0
39	233561.0
40	423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12434756889027	20.163929686810828	13.419380233996767	24.292342510302127
2	14.944860030162225	20.731176486060065	40.25175196145222	24.072211522325492
3	17.541627783690746	26.304587900963906	30.562445102300973	25.591339213044378
4	12.197390750600967	17.88443863573976	39.907394132837815	30.010776480821455
5	13.185072015938754	38.56289683414384	35.29501547234076	12.957015677576647
6	29.639234168635976	38.700593959785	18.630399049249426	13.0297728223296
7	26.33156391573473	32.85326922435796	23.380353167356283	17.434813692551035
8	25.208492081690594	35.445746865256304	21.129074500009818	18.21668655304328
9	26.163774102131914	15.615254959343568	20.869362277500972	37.35160866102355
10-11	24.291373869614606	26.61848183705929	28.965297402283007	20.1248468910431
12-13	24.751842014602758	24.521002115214355	28.3006049290792	22.42655094110369
14-15	22.419614993642995	25.724980217708932	25.82996713324225	26.025437655405824
16-17	22.261547782686065	28.38453013786194	26.57238904817305	22.781533031278947
18-19	22.062179599619107	28.42202007850105	27.70400464241942	21.811795679460424
20-21	23.378800333547325	27.548215999676973	27.01273992236245	22.06024374441325
22-23	23.35475637922125	27.350648340393057	26.691602598177127	22.602992682208562
24-25	22.917234275964525	27.52486661220087	27.07232881289443	22.48557029894018
26-27	22.68735154790252	27.681843169388383	26.841676532603525	22.789128750105576
28-29	22.609397877564025	27.79181642475391	26.91407736824607	22.684708329435995
30-31	22.992145527692546	27.516028921054264	26.841327804980146	22.65049774627304
32-33	22.903980102082777	27.79727403868595	26.52872254274874	22.770023316482536
34-35	23.126660241647645	27.75986086987447	26.84478651938888	22.268692369089003
36-37	23.060297040358876	27.506418116064584	26.666871666020487	22.766413177556053
38-39	22.848952005211956	27.651579915889222	26.888051308944554	22.611416769954264
40-41	22.910476114262384	27.259381436576792	27.099184250289948	22.730958198870876
42-43	22.784868281504576	27.35863684486941	26.901417452364875	22.955077421261137
44-45	22.887188587640267	27.58182074512025	26.914427495156882	22.6165631720826
46-47	22.860903173598047	27.469884517950348	26.75167413216168	22.917538176289924
48-49	23.05533411354204	27.282707952885865	26.876664713365933	22.785293220206157
50-51	22.846586899544224	27.425597424350578	26.685735533138715	23.042080142966476
52-53	22.766047753417343	27.46313374599157	26.74843872940391	23.022379771187175
54-55	22.768868712822197	27.362621156140005	26.786377968249003	23.082132162788792
56-57	23.08412047845874	27.28752269444033	26.691348385575093	22.937008441525837
58-59	22.81581872753548	27.323563197094895	26.954755993626563	22.905862081743063
60-61	22.862689500613634	27.48909463617661	26.818820516164987	22.829395347044766
62-63	22.943258331522344	27.297802376351132	26.68918036395044	23.069758928176082
64-65	22.685994204702936	27.25529321090583	26.835134173725518	23.22357841066572
66-67	22.932786843027255	27.2364350586056	26.67226941512605	23.158508683241095
68-69	22.749369074623633	27.212490997698634	26.820141661529775	23.21799826614796
70-71	22.714277879599294	27.05669782577923	26.847016736690062	23.38200755793142
72-73	22.8766631934203	27.100733531846906	26.953367591498793	23.069235683234
74-75	22.87948053892271	27.389450920332926	26.74442303038972	22.98664551035465
76-77	22.811559773464634	27.249739360565822	26.63172699401905	23.30697387195049
78-79	22.753151804465375	27.271239998480723	26.68400247901499	23.29160571803891
80-81	22.68324303452181	27.3054452953365	26.87663942786851	23.134672242273187
82-83	22.989602723891057	27.00757177829792	27.246618157038014	22.756207340773006
84-85	23.0821790909832	27.247548856132862	26.678060872851777	22.992211180032164
86-87	22.56118301993339	27.441614532152954	26.89070056632829	23.106501881585366
88-89	22.96525118842703	27.033424168638348	26.76169636553179	23.239628277402833
90-91	22.885042786031306	26.997441064066773	26.91093895088814	23.206577199013775
92-93	22.97256812946632	27.029571757640277	26.893156270489882	23.10470384240352
94-95	22.619491843535506	27.031112142076598	27.12084195953765	23.228554054850246
96-97	22.682675134690488	27.009185632026323	26.97980198460045	23.328337248682736
98-99	22.492733002451047	27.220699966096607	26.87803486306179	23.408532168390554
100	22.91102085218359	26.7930439860358	26.627050556115044	23.668884605665564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1060.0
1	768.0
2	505.5
3	523.0
4	454.5
5	403.0
6	376.0
7	348.5
8	367.0
9	394.0
10	358.5
11	305.0
12	304.5
13	334.5
14	364.5
15	426.0
16	497.5
17	567.0
18	704.5
19	935.0
20	1347.5
21	2006.5
22	3042.0
23	4491.0
24	6321.0
25	9097.0
26	13092.5
27	17331.0
28	20819.5
29	25273.5
30	30977.5
31	36689.0
32	43413.5
33	50290.0
34	56480.0
35	61089.0
36	65748.5
37	72474.5
38	75574.0
39	76982.5
40	78910.5
41	79423.5
42	80416.0
43	83028.5
44	87894.0
45	96992.0
46	104299.5
47	108374.0
48	111956.0
49	111205.0
50	108729.0
51	108451.0
52	105946.5
53	101879.0
54	98031.5
55	89186.5
56	80393.0
57	70799.5
58	61531.0
59	55412.5
60	46221.5
61	36422.5
62	29052.0
63	22359.5
64	16085.5
65	11193.0
66	8242.0
67	6243.5
68	4781.5
69	3585.0
70	2594.5
71	1911.5
72	1503.5
73	1230.5
74	987.5
75	733.5
76	520.0
77	352.5
78	219.0
79	148.5
80	111.5
81	70.5
82	47.0
83	41.0
84	29.5
85	16.5
86	15.0
87	14.5
88	10.0
89	6.0
90	6.0
91	4.5
92	3.5
93	3.5
94	4.0
95	3.0
96	0.0
97	1.0
98	4.0
99	5.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06200780839068624
2	0.00951971729773379
3	0.011890386196780334
4	0.009741967507019403
5	0.012112636406065948
6	0.01181630279368513
7	0.0033337531392842058
8	0.00581554714297356
9	0.007778757324996481
10-11	0.007167569249461043
12-13	0.006297089263092389
14-15	0.010223509627138232
16-17	0.009501196446959988
18-19	0.007612069668032271
20-21	0.007149048398687243
22-23	0.002778127616070172
24-25	0.003944941214819644
26-27	0.0050376714104739114
28-29	0.006204485009223383
30-31	0.008982612625293556
32-33	0.010445759836423846
34-35	0.011723698539816125
36-37	0.017205870368861263
38-39	0.017835579295170503
40-41	0.018020787802908514
42-43	0.02324366772112044
44-45	0.026910796174333063
46-47	0.02959631953653423
48-49	0.029985257402784054
50-51	0.03131875865849774
52-53	0.030244549313617274
54-55	0.03604157560581703
56-57	0.03839372365408977
58-59	0.0355600334856982
60-61	0.036597201129031066
62-63	0.031800300778616564
64-65	0.0340783654237941
66-67	0.036467555173614455
68-69	0.038690057266470596
70-71	0.03844928620641118
72-73	0.03467103264855575
74-75	0.03767141047391153
76-77	0.037356556010756915
78-79	0.03731951430920931
80-81	0.04146818488254077
82-83	0.03780105642932814
84-85	0.0340783654237941
86-87	0.032300363749509196
88-89	0.030355674418260075
90-91	0.03267078076498522
92-93	0.03405984457302031
94-95	0.0371528266522451
96-97	0.03259669736189001
98-99	0.030188986761295864
100	0.02807760977308254
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2699660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.30856762885466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9593854040123	41.37540122731813
2	15.197399275415219	17.722771668665104
3	6.071543110769222	10.620689462573814
4	2.9701425123416665	6.927390221928414
5	1.5939344057119984	4.647001604570815
6	0.93687378640927	3.2776661120727635
7	0.6099557332025136	2.4895951584032474
8	0.4172371615561112	1.9462801001492693
9	0.2708274006224873	1.4212402024448891
>10	0.9505936147708212	8.444094723710355
>50	0.017824284795317395	0.70044839337456
>100	0.004183741104394137	0.3542107105935393
>500	0.0	0.0
>1k	9.956928864505378E-5	0.07321041419504222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.704170154760229E-5	0.0
3	0.0	0.0	0.0	3.704170154760229E-5	0.0
4	0.0	0.0	0.0	3.704170154760229E-5	0.0
5	0.0	0.0	0.0	3.704170154760229E-5	0.0
6	7.408340309520458E-5	0.0	0.0	3.704170154760229E-5	0.0
7	7.408340309520458E-5	0.0	0.0	3.704170154760229E-5	0.0
8	7.408340309520458E-5	0.0	0.0	3.704170154760229E-5	0.0
9	7.408340309520458E-5	0.0	0.0	3.704170154760229E-5	0.0
10-11	7.408340309520458E-5	0.0	0.0	7.408340309520458E-5	0.0
12-13	7.408340309520458E-5	0.0	0.0	7.408340309520458E-5	0.0
14-15	7.408340309520458E-5	0.0	0.0	7.408340309520458E-5	0.0
16-17	7.408340309520458E-5	0.0	0.0	7.408340309520458E-5	0.0
18-19	7.408340309520458E-5	0.0	0.0	7.408340309520458E-5	0.0
20-21	7.408340309520458E-5	0.0	0.0	7.408340309520458E-5	0.0
22-23	7.408340309520458E-5	0.0	0.0	1.666876569642103E-4	0.0
24-25	7.408340309520458E-5	0.0	0.0	2.59291910833216E-4	1.8520850773801146E-5
26-27	7.408340309520458E-5	0.0	0.0	6.852714786306424E-4	3.704170154760229E-5
28-29	7.408340309520458E-5	0.0	0.0	0.0027781276160701715	3.704170154760229E-5
30-31	7.408340309520458E-5	0.0	0.0	0.008315861997436714	3.704170154760229E-5
32-33	7.408340309520458E-5	0.0	0.0	0.02070631116510968	3.704170154760229E-5
34-35	1.1112510464280687E-4	0.0	0.0	0.0403754546868865	3.704170154760229E-5
36-37	1.1112510464280687E-4	0.0	0.0	0.07186090100234845	3.704170154760229E-5
38-39	1.1112510464280687E-4	0.0	0.0	0.11405139906506745	3.704170154760229E-5
40-41	1.4816680619040917E-4	0.0	0.0	0.15709385626338132	3.704170154760229E-5
42-43	1.8520850773801146E-4	0.0	0.0	0.2021365653452657	3.704170154760229E-5
44-45	1.8520850773801146E-4	0.0	0.0	0.25575442833542	3.704170154760229E-5
46-47	1.8520850773801146E-4	0.0	0.0	0.32250357452419937	3.704170154760229E-5
48-49	1.8520850773801146E-4	0.0	0.0	0.39566093508071387	3.704170154760229E-5
50-51	2.2225020928561375E-4	0.0	0.0	0.4706333390130609	3.704170154760229E-5
52-53	2.2225020928561375E-4	0.0	0.0	0.5404939881318389	3.704170154760229E-5
54-55	2.2225020928561375E-4	0.0	0.0	0.6144107035700792	3.704170154760229E-5
56-57	2.2225020928561375E-4	0.0	0.0	0.7041997881214672	3.704170154760229E-5
58-59	2.2225020928561375E-4	0.0	0.0	0.8057866546157664	3.704170154760229E-5
60-61	2.4077106005941488E-4	0.0	0.0	0.9099849610691717	3.704170154760229E-5
62-63	2.59291910833216E-4	0.0	0.0	1.0139610173132914	3.704170154760229E-5
64-65	2.7781276160701715E-4	0.0	0.0	1.1208633679796716	3.704170154760229E-5
66-67	3.333753139284206E-4	0.0	0.0	1.238896749961106	3.704170154760229E-5
68-69	3.333753139284206E-4	0.0	0.0	1.3697280398272376	3.704170154760229E-5
70-71	3.333753139284206E-4	0.0	0.0	1.5121533822777682	3.704170154760229E-5
72-73	4.074587170236252E-4	0.0	0.0	1.6527636813524667	1.4816680619040917E-4
74-75	4.074587170236252E-4	0.0	0.0	1.795596482520021	1.4816680619040917E-4
76-77	4.074587170236252E-4	0.0	0.0	1.952079150707867	1.4816680619040917E-4
78-79	4.074587170236252E-4	0.0	0.0	2.1190260995829107	1.4816680619040917E-4
80-81	4.074587170236252E-4	0.0	0.0	2.299511790373603	1.4816680619040917E-4
82-83	4.074587170236252E-4	0.0	0.0	2.483960943229888	1.4816680619040917E-4
84-85	4.074587170236252E-4	0.0	0.0	2.6685212211908165	1.4816680619040917E-4
86-87	4.074587170236252E-4	0.0	0.0	2.8603046309535274	1.4816680619040917E-4
88	4.074587170236252E-4	0.0	0.0	3.0155649229903023	1.4816680619040917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3970	0.0	37.543648	1
CAACGCA	4075	0.0	34.711487	5
ATCAACG	4135	0.0	34.32146	3
GGTATCA	2490	0.0	33.800365	1
TATCAAC	4935	0.0	30.090837	2
AACGCAG	4860	0.0	29.781103	6
TCAACGC	4895	0.0	28.992697	4
ACGCAGA	5190	0.0	27.252186	7
CGCAGAG	5265	0.0	26.864473	8
GTACATG	8750	0.0	25.148119	1
TACATGG	8695	0.0	24.482906	2
ACATGGG	8555	0.0	23.89481	3
GTACTAG	1260	0.0	22.762869	1
TGATCGC	770	0.0	22.579859	9
GTGATCG	770	0.0	21.969593	8
CATGGGG	6520	0.0	21.622604	4
GCAGAGT	6415	0.0	21.023037	9
TAGTACT	2000	0.0	20.911942	4
CTAACGC	485	0.0	20.347542	3
GTATAAG	1760	0.0	20.303394	1
>>END_MODULE