FastQCFastQC Report
Mon 16 Jan 2017
ERR1146555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699660
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102420.3793811072505427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89590.3318566041649689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.18335642266063135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53400.060.0064161
GTATCAA96350.041.9989621
ATCAACG120600.032.4367833
CAACGCA121500.032.196515
TATCAAC129450.031.1139762
TCAACGC127800.030.682944
AACGCAG130800.030.255566
GTGGTAT14750.028.7106041
ACGCAGA145000.026.9988777
CGCAGAG145000.026.9988778
GTACTAG11500.026.186291
GCAGAGT159250.024.2583379
GTACATG84100.023.4428181
TACATGG85350.022.4926382
GAGTACT114850.022.36288512-13
CAGAGTA156350.022.18335210-11
AGAGTAC150550.021.83612610-11
ACATGGG84450.021.8246963
TGGTATC19700.020.5407352
GTACTTT125350.020.37717214-15
Produced by FastQC (version 0.11.5)