FastQCFastQC Report
Mon 16 Jan 2017
ERR1146554_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146554_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583868
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2713247.570702938835917No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC661301.8452130491413188No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT624021.7411913608425311No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC486971.3587833034029155No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA460181.284031666344854No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA430841.2021648118736517No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG411861.1492052720691723No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC351950.9820395170804281No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG234300.6537629176074565No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA117420.3276348347651197No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG105440.2942072643300479No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA83840.23393718741873304No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC68340.1906878266721877No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA64650.18039168853317142No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA62030.173081151426336No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42530.11867066532584349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA47000.027.3954053
TACGCAG47200.027.3785024
GAGTACG48750.026.6177671
CGCAGTG49750.026.0689056
GTACTAG7250.025.9393521
AGTACGC50450.025.4288352
GTCGTAA1501.1604334E-625.0747091
AGTAGGC25300.022.474492
ACGCAGT58800.022.1371335
GCTTATG22800.022.0640951
TACCTGG25300.021.7315312
GTAGGCA27750.020.8289383
GTACCTG29000.020.427241
TAGGCAG27650.020.0542224
CTAGGCA11250.020.049694
GCATCGT8050.019.8462188
GGGCTAT12100.019.4174186
GGCTATT11800.019.1133047
TTTAGGG12800.019.0905482
TATGTAC26100.018.904614
Produced by FastQC (version 0.11.5)