##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146554_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3583868
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.640468064113968	31.0	31.0	34.0	30.0	34.0
2	31.75941329312352	33.0	31.0	34.0	30.0	34.0
3	31.9655640218892	34.0	31.0	34.0	30.0	34.0
4	35.39288974928764	37.0	35.0	37.0	33.0	37.0
5	35.488981178994315	37.0	35.0	37.0	33.0	37.0
6	35.636464289421376	37.0	35.0	37.0	35.0	37.0
7	35.61266988627929	37.0	35.0	37.0	35.0	37.0
8	35.60299235351302	37.0	36.0	37.0	35.0	37.0
9	37.26749199468284	39.0	38.0	39.0	35.0	39.0
10-11	37.24247056532216	39.0	38.0	39.0	35.0	39.0
12-13	37.23189665467589	39.0	38.0	39.0	35.0	39.0
14-15	38.4919498988244	40.0	38.5	41.0	34.5	41.0
16-17	38.627471910237766	40.5	39.0	41.0	35.0	41.0
18-19	38.72477125273587	41.0	39.0	41.0	35.0	41.0
20-21	38.74042124319311	41.0	39.0	41.0	35.0	41.0
22-23	38.692586055066755	40.5	39.0	41.0	34.5	41.0
24-25	38.59160326217372	40.5	38.5	41.0	34.5	41.0
26-27	38.433327064501256	40.0	38.0	41.0	34.0	41.0
28-29	38.43993472973893	40.0	38.0	41.0	34.0	41.0
30-31	38.515429558231496	40.0	38.0	41.0	34.0	41.0
32-33	38.386348492745825	40.0	38.0	41.0	34.0	41.0
34-35	38.054785499912384	40.0	38.0	41.0	33.0	41.0
36-37	37.86346400034823	40.0	37.5	41.0	32.5	41.0
38-39	37.743198131181174	40.0	38.0	41.0	32.0	41.0
40-41	37.89444519161978	40.0	38.0	41.0	33.0	41.0
42-43	37.700185804834334	40.0	37.5	41.0	32.0	41.0
44-45	37.44999913501279	40.0	37.0	41.0	31.0	41.0
46-47	37.1377405641056	40.0	36.5	41.0	30.0	41.0
48-49	36.81739812961861	39.0	36.0	41.0	30.0	41.0
50-51	36.573058215313736	39.0	35.0	41.0	28.5	41.0
52-53	36.66161169998448	39.0	35.5	40.5	29.5	41.0
54-55	36.442427288058596	39.0	35.5	40.5	28.5	41.0
56-57	35.81556142692755	38.5	34.5	40.0	27.5	41.0
58-59	35.426785668445376	38.0	34.0	40.0	27.0	41.0
60-61	34.94793934932871	38.0	33.0	40.0	26.0	41.0
62-63	34.97721121425231	37.0	33.5	40.0	26.0	41.0
64-65	34.830257141167024	37.0	33.5	39.5	26.0	41.0
66-67	34.27878830916764	36.0	32.5	39.0	26.0	40.5
68-69	33.63265597393654	35.5	32.0	39.0	25.0	40.0
70-71	32.95986668593821	35.0	31.0	37.5	24.0	40.0
72-73	32.55983869941639	35.0	31.0	37.0	23.5	39.0
74-75	32.45220834026253	35.0	31.0	36.5	24.5	39.0
76-77	32.32379247784796	34.5	31.0	36.0	25.0	38.5
78-79	31.86227729369497	34.0	30.5	35.5	23.5	37.0
80-81	31.181111302090365	34.0	29.0	35.0	20.0	37.0
82-83	30.901415732945523	34.0	29.0	35.0	20.5	36.5
84-85	30.823923760584933	34.0	29.0	35.0	20.0	36.0
86-87	31.0768123993406	34.0	30.0	35.0	21.0	36.0
88-89	30.982854000203133	34.0	30.0	35.0	20.0	35.5
90-91	30.481609116183968	34.0	29.0	35.0	20.0	35.0
92-93	30.145943851726685	34.0	29.0	35.0	19.0	35.0
94-95	30.059819027932946	34.0	29.0	35.0	18.5	35.0
96-97	29.965177288895685	34.0	29.0	35.0	18.5	35.0
98-99	30.25942696550208	34.0	29.0	35.0	19.0	35.0
100	29.126937152819245	33.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17130116439290788
1101	2	0.1782520822703475
1101	3	0.2525054067749224
1101	4	0.32843254490313
1101	5	0.2533139024936588
1101	6	0.20871025429791956
1101	7	0.18004465931473135
1101	8	0.2961683836296558
1101	9	0.37812324701595657
1101	10-11	0.39209891674561703
1101	12-13	0.3625344900429539
1101	14-15	0.4984043293212821
1101	16-17	0.4566292715875804
1101	18-19	0.36445606536516806
1101	20-21	0.42966186234252746
1101	22-23	0.43767495512545196
1101	24-25	0.42209606630444796
1101	26-27	0.4618890104116602
1101	28-29	0.5012711889475838
1101	30-31	0.507507523773171
1101	32-33	0.44896846217487507
1101	34-35	0.4490190420265634
1101	36-37	0.49517505938682405
1101	38-39	0.48152598610168695
1101	40-41	0.4547588581418154
1101	42-43	0.4388037752251108
1101	44-45	0.4083213106001722
1101	46-47	0.44360017658919304
1101	48-49	0.4960974790495669
1101	50-51	0.5685409996711428
1101	52-53	0.48001127971362934
1101	54-55	0.5152041206080753
1101	56-57	0.48733059930074774
1101	58-59	0.5367625674273242
1101	60-61	0.5497730471178954
1101	62-63	0.5048701050139002
1101	64-65	0.47354194034053165
1101	66-67	0.47319742501515805
1101	68-69	0.5376917752444612
1101	70-71	0.5168476837314131
1101	72-73	0.5455320189333861
1101	74-75	0.47920082103926376
1101	76-77	0.4635508154089081
1101	78-79	0.42007522149306453
1101	80-81	0.441978329895246
1101	82-83	0.4110476611548144
1101	84-85	0.4329368590811953
1101	86-87	0.3991338380643157
1101	88-89	0.35968726386209227
1101	90-91	0.48019998589338186
1101	92-93	0.4972564460529796
1101	94-95	0.5324070513701109
1101	96-97	0.41484044697107336
1101	98-99	0.458419191189094
1101	100	0.4393490899209844
1102	1	0.18280908838631404
1102	2	0.16161387908823954
1102	3	0.2607646195312512
1102	4	0.13770784300545813
1102	5	0.13497611259941067
1102	6	0.13304919971506024
1102	7	0.17357767539024138
1102	8	0.19442399558901258
1102	9	0.2616572741959544
1102	10-11	0.2732639858349799
1102	12-13	0.2334376611073381
1102	14-15	0.3485910366616949
1102	16-17	0.33904401224366865
1102	18-19	0.31447317771574035
1102	20-21	0.27262865990227425
1102	22-23	0.3235520259021527
1102	24-25	0.3627693125586333
1102	26-27	0.42156408130976075
1102	28-29	0.4287880638950412
1102	30-31	0.46822271022004713
1102	32-33	0.4572293220388701
1102	34-35	0.47163372047029384
1102	36-37	0.5515042967953008
1102	38-39	0.4585075905883542
1102	40-41	0.41073109923867435
1102	42-43	0.42895411530672334
1102	44-45	0.4923690201498232
1102	46-47	0.5528734937700293
1102	48-49	0.5349968913703407
1102	50-51	0.545357893391234
1102	52-53	0.5373180206181374
1102	54-55	0.5603582156559312
1102	56-57	0.5381931083279952
1102	58-59	0.5766830108093828
1102	60-61	0.5826034167262222
1102	62-63	0.5608490575254592
1102	64-65	0.5768062573737041
1102	66-67	0.5141040686217337
1102	68-69	0.5544059210706749
1102	70-71	0.5416036241020734
1102	72-73	0.6129243514583322
1102	74-75	0.47594789320655906
1102	76-77	0.5587080245883342
1102	78-79	0.6247561960642685
1102	80-81	0.5944427192078976
1102	82-83	0.599287338774996
1102	84-85	0.5559908892198422
1102	86-87	0.5141599093629061
1102	88-89	0.5013999053299756
1102	90-91	0.605526541459195
1102	92-93	0.612611280041655
1102	94-95	0.5774279283499695
1102	96-97	0.5821676330936221
1102	98-99	0.4808623727826742
1102	100	0.30665247423969433
1103	1	0.1467373047792826
1103	2	0.11822287628829642
1103	3	0.1509981330159178
1103	4	0.12136777465791937
1103	5	0.08469678081986132
1103	6	0.06080858398887301
1103	7	0.06762971109287008
1103	8	0.1326907376553521
1103	9	0.17034451045594068
1103	10-11	0.22523263519382652
1103	12-13	0.1774404537559846
1103	14-15	0.2479005287271363
1103	16-17	0.20975555699739346
1103	18-19	0.13387871845187505
1103	20-21	0.11718409806616137
1103	22-23	0.18915671648814225
1103	24-25	0.2175079802992741
1103	26-27	0.21494078370061942
1103	28-29	0.18685009490367577
1103	30-31	0.20053560809250826
1103	32-33	0.26130758717411595
1103	34-35	0.2702102242916027
1103	36-37	0.2705277219662534
1103	38-39	0.2784271905585314
1103	40-41	0.2901680305060097
1103	42-43	0.2736187978033229
1103	44-45	0.3215022927530029
1103	46-47	0.3384592253593155
1103	48-49	0.41752020708110393
1103	50-51	0.3433513074754373
1103	52-53	0.3254547077488965
1103	54-55	0.40275717371410025
1103	56-57	0.44538243879384964
1103	58-59	0.5521752395245727
1103	60-61	0.5058360793527399
1103	62-63	0.4424983106469256
1103	64-65	0.3886298183435031
1103	66-67	0.4132715927013564
1103	68-69	0.47220514056405705
1103	70-71	0.41247766467413527
1103	72-73	0.468176862577792
1103	74-75	0.42510800697608175
1103	76-77	0.43103196475102123
1103	78-79	0.5305696868694554
1103	80-81	0.5154097437380756
1103	82-83	0.46543259561746453
1103	84-85	0.472697924412401
1103	86-87	0.47411278471216534
1103	88-89	0.38593048042486444
1103	90-91	0.4577464592589351
1103	92-93	0.44858949472289567
1103	94-95	0.3452339235390731
1103	96-97	0.3559477671854836
1103	98-99	0.2556317282298828
1103	100	0.3323164524870137
1104	1	0.09290429438568637
1104	2	0.11273848669533848
1104	3	0.2241718692846817
1104	4	0.11152700368425883
1104	5	0.20180219393725451
1104	6	0.18179917904200948
1104	7	0.11303316353946258
1104	8	0.18529822520252992
1104	9	0.19422144569389133
1104	10-11	0.2341896696880852
1104	12-13	0.18807654308925237
1104	14-15	0.31935809637821677
1104	16-17	0.2576112073619825
1104	18-19	0.2086779614116523
1104	20-21	0.2025682675194247
1104	22-23	0.2766841864454861
1104	24-25	0.22393871060505433
1104	26-27	0.2807341135621826
1104	28-29	0.24270544237455738
1104	30-31	0.23771567003694116
1104	32-33	0.3090014812985231
1104	34-35	0.38544250338203767
1104	36-37	0.3904967784543558
1104	38-39	0.3501573908528641
1104	40-41	0.3709356784817288
1104	42-43	0.3147129281702803
1104	44-45	0.34663339475044097
1104	46-47	0.34563441440207043
1104	48-49	0.4834089694857653
1104	50-51	0.43211838976925776
1104	52-53	0.3582916692236111
1104	54-55	0.43294525041542187
1104	56-57	0.5419815391121929
1104	58-59	0.6094664212543819
1104	60-61	0.5620482931900384
1104	62-63	0.5032186963682506
1104	64-65	0.4606811862389293
1104	66-67	0.42657906552026503
1104	68-69	0.480467059858249
1104	70-71	0.5580037421774904
1104	72-73	0.5732825986416543
1104	74-75	0.5639499050685188
1104	76-77	0.4660388491952432
1104	78-79	0.5061624450575479
1104	80-81	0.6923570416467797
1104	82-83	0.5989879027685525
1104	84-85	0.5131573000058225
1104	86-87	0.42160976557904206
1104	88-89	0.39816513055210123
1104	90-91	0.5446786128862051
1104	92-93	0.6345604338256123
1104	94-95	0.6008372045749972
1104	96-97	0.40735277638933454
1104	98-99	0.4024178953343096
1104	100	0.4362350732096232
1105	1	0.04416518048345708
1105	2	0.06579761664020012
1105	3	0.15228146567443446
1105	4	0.15181969932999806
1105	5	0.07596091803889493
1105	6	0.10423350002852061
1105	7	0.019241693181115238
1105	8	0.0907290721327314
1105	9	0.10914211089996684
1105	10-11	0.11181906169903044
1105	12-13	0.08597171905274337
1105	14-15	0.18065124670494725
1105	16-17	0.12107214216273832
1105	18-19	0.08854142682062616
1105	20-21	0.10140337587083792
1105	22-23	0.1165995425471138
1105	24-25	0.20126763650807789
1105	26-27	0.18134648019620414
1105	28-29	0.2335288537729454
1105	30-31	0.16968249180334283
1105	32-33	0.18263399377492817
1105	34-35	0.1869739329059712
1105	36-37	0.215448004333318
1105	38-39	0.23650599473810985
1105	40-41	0.2391156882732517
1105	42-43	0.19469206690924068
1105	44-45	0.16940848685853638
1105	46-47	0.25249070720344946
1105	48-49	0.24056014222875177
1105	50-51	0.29553630509885664
1105	52-53	0.27853064931245086
1105	54-55	0.3431699977612084
1105	56-57	0.356852730054392
1105	58-59	0.47396272502441406
1105	60-61	0.5015304181317575
1105	62-63	0.389104850298736
1105	64-65	0.37447276817084685
1105	66-67	0.3129898960034794
1105	68-69	0.3707692991621272
1105	70-71	0.38436955609764567
1105	72-73	0.4386164529511447
1105	74-75	0.41661937370429314
1105	76-77	0.21649397947498272
1105	78-79	0.2519589859476099
1105	80-81	0.36395246422528515
1105	82-83	0.47114457406639687
1105	84-85	0.4539743951424313
1105	86-87	0.3955552044592672
1105	88-89	0.2351787128736973
1105	90-91	0.3333396013321277
1105	92-93	0.47821900808919793
1105	94-95	0.3930470747468
1105	96-97	0.3491904888227815
1105	98-99	0.2507173869332675
1105	100	0.11592186300458351
1106	1	0.14676196163735966
1106	2	0.1647350349673502
1106	3	0.16825567085542303
1106	4	0.15618013713839218
1106	5	0.12422843472465672
1106	6	0.13173013483970664
1106	7	0.13664964077868547
1106	8	0.16577025360377462
1106	9	0.23305505446971608
1106	10-11	0.21168716392909204
1106	12-13	0.17987898580837935
1106	14-15	0.28425870454723423
1106	16-17	0.2702022152011452
1106	18-19	0.19458208888051587
1106	20-21	0.21314213287399753
1106	22-23	0.2184694877184512
1106	24-25	0.27726186662195573
1106	26-27	0.22702785252844393
1106	28-29	0.25415217894658326
1106	30-31	0.26339703881293985
1106	32-33	0.27797314584272215
1106	34-35	0.3149473232733584
1106	36-37	0.33972290261168325
1106	38-39	0.2866008804893383
1106	40-41	0.3959721105449745
1106	42-43	0.2633411600208433
1106	44-45	0.2726949980371458
1106	46-47	0.28800548064971565
1106	48-49	0.33217728129363877
1106	50-51	0.28625566063592345
1106	52-53	0.2995988124150699
1106	54-55	0.4398864420522699
1106	56-57	0.4335093033224382
1106	58-59	0.508729309400394
1106	60-61	0.41249707494204557
1106	62-63	0.4400153806443967
1106	64-65	0.3731170458179207
1106	66-67	0.419724081783869
1106	68-69	0.35568625118698094
1106	70-71	0.3389205024693851
1106	72-73	0.4021902531244024
1106	74-75	0.3427356668834136
1106	76-77	0.3379413820868393
1106	78-79	0.3483287225740739
1106	80-81	0.4328141849532017
1106	82-83	0.39510312147256244
1106	84-85	0.46641522432726745
1106	86-87	0.37537230930141163
1106	88-89	0.2759647362914208
1106	90-91	0.4404926472579547
1106	92-93	0.38435137642194306
1106	94-95	0.39705224197724576
1106	96-97	0.32738685456338246
1106	98-99	0.3076480427989594
1106	100	0.3474476484296787
1107	1	0.03635208241953691
1107	2	0.05757278130279175
1107	3	0.026902600597146886
1107	4	0.08237089227295513
1107	5	0.08237112197075191
1107	6	0.05788952019693028
1107	7	-0.0025205624569224483
1107	8	0.029128838540863455
1107	9	0.0560882174459536
1107	10-11	0.10366501558589647
1107	12-13	0.07853305164094593
1107	14-15	0.11469534837371498
1107	16-17	0.09151030717391251
1107	18-19	0.06350502144680092
1107	20-21	0.08984871811582451
1107	22-23	0.10072895034777218
1107	24-25	0.13044250596362872
1107	26-27	0.13891991104524237
1107	28-29	0.119427467948654
1107	30-31	0.13500166054144103
1107	32-33	0.16592750345690632
1107	34-35	0.08783984508121989
1107	36-37	0.20076524562248466
1107	38-39	0.1735262240470803
1107	40-41	0.14592100128190566
1107	42-43	0.14313713483026902
1107	44-45	0.13318820989475455
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2216	96-97	-2.0594982050087935
2216	98-99	-2.021373554663363
2216	100	-1.9174382272487662
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	85.0
1	55.0
2	47.0
3	47.0
4	32.0
5	28.0
6	115.0
7	539.0
8	1515.0
9	1802.0
10	1979.0
11	2602.0
12	3790.0
13	4988.0
14	6072.0
15	6838.0
16	7503.0
17	8431.0
18	9341.0
19	10841.0
20	12834.0
21	15064.0
22	18142.0
23	21640.0
24	26484.0
25	32693.0
26	38538.0
27	43062.0
28	50226.0
29	62807.0
30	77703.0
31	97158.0
32	128540.0
33	179441.0
34	267046.0
35	398421.0
36	570125.0
37	735776.0
38	583789.0
39	157433.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.95313810919132	16.890382905300424	12.947473193557798	19.20900579195046
2	12.863431107777362	27.002188330355843	31.55152678039734	28.58285378146946
3	31.846988185812258	21.679715405772253	29.482001893127592	16.991294515287898
4	10.597709309321376	14.427154317452985	45.7900103472871	29.18512602593854
5	10.445257994130275	43.26492762747201	34.433662539106614	11.856151839291101
6	26.786176727323056	37.65962208633881	15.435539466839906	20.118661719498228
7	24.800070317208743	33.255630957430185	23.078889211331116	18.865409514029956
8	22.732182615788197	22.9972734707955	20.608023546764954	33.66252036665135
9	18.69400397642052	24.923645749764553	27.807848198402457	28.574502075412468
10-11	27.098775948022542	21.165672632823053	26.893104632299565	24.84244678685484
12-13	34.35457739758213	21.466049158791087	23.539088058773565	20.640285384853215
14-15	24.343489802254073	24.73240989113335	32.420034812212876	18.5040654943997
16-17	19.66757615226896	27.510755812533876	30.472948791379444	22.34871924381772
18-19	18.17622070785303	36.546562015762376	24.68109555845705	20.596121717927545
20-21	23.46988306161934	25.793972859156174	21.745490862013327	28.99065321721116
22-23	19.875045360618486	27.22189598386397	24.962675587971976	27.94038306754557
24-25	28.318874962085665	26.361155966523214	22.229616738058624	23.090352333332493
26-27	25.132377299860252	20.516849085413494	33.28105955415861	21.069714060567637
28-29	19.20134287555185	35.28809848498167	25.002099812677177	20.508458826789315
30-31	22.423689817262975	24.92538854464758	24.301624220573647	28.349297417515796
32-33	24.129513656148685	26.48112997465616	26.75593007310701	22.633426296088142
34-35	28.466641709440683	23.797528899756088	25.845253943495184	21.890575447308045
36-37	24.615368514763894	21.52524185547417	32.96628605157072	20.893103578191216
38-39	22.195272551453336	28.401683121826792	29.059749987615895	20.343294339103977
40-41	21.496945626883953	30.460705348229794	25.872782687541356	22.169566337344897
42-43	19.999413901421008	29.83566912223365	20.537410532749654	29.627506443595685
44-45	23.87149795317663	23.182287714119607	23.479828595463577	29.466385737240188
46-47	28.773987184505856	28.269308196375853	25.234075007553507	17.722629611564784
48-49	25.610943198579516	26.188912134737908	26.367952157000335	21.832192509682244
50-51	15.751570911704471	28.4023069113137	34.59311904039188	21.253003136589943
52-53	22.580632556140298	31.771327452400588	22.432255534482444	23.215784456976664
54-55	19.11555090771327	24.929446135590982	26.91512866615967	29.039874290536076
56-57	26.299678013200484	21.259935602640095	26.11907510094363	26.321311283215792
58-59	32.63474533124022	22.91812280462778	23.28392513013128	21.16320673400072
60-61	24.132149118209885	25.6386891493919	32.75688982051289	17.47227191188533
62-63	19.82662456605551	26.958044763881862	30.216506463374216	22.998824206688415
64-65	18.218544998400603	35.638771794961976	24.356709133102996	21.78597407353443
66-67	23.333456617387938	25.388899550245814	21.702548141841792	29.575095690524456
68-69	19.977121645655995	26.7157404948128	25.335418698867567	27.971719160663632
70-71	28.011665845760557	25.84178001288792	22.629206178443166	23.51734796290835
72-73	24.902601385624823	20.59103356346133	32.99373089971826	21.512634151195588
74-75	19.499850381638478	34.48654383817993	24.992421383366906	21.021184396814693
76-77	22.122172053692964	24.61580739937035	24.691369569213286	28.570650977723393
78-79	23.961426351309388	26.04250247208826	26.90163775410869	23.094433422493665
80-81	28.12000716295685	23.4948613600769	25.832536712596404	22.552594764369847
82-83	24.10835659930004	21.4733009424182	32.68201187666888	21.73633058161288
84-85	21.829535147495143	28.128993684631272	28.784423755924276	21.257047411949305
86-87	21.36905772889677	29.98390724679104	25.90622046108927	22.740814563222916
88-89	20.001702599185126	29.301885489040213	20.962708193339907	29.73370371843475
90-91	23.60489115864334	22.910548380348335	23.75360712273547	29.730953338272855
92-93	28.247631680094052	27.64996568137748	25.31791828455455	18.784484353973912
94-95	24.824852207601197	25.906395146706558	26.520593517134593	22.74815912855765
96-97	16.330337225426554	27.714402866259817	33.9509072618912	22.004352646422422
98-99	22.757607500613357	30.887905286083555	22.871626157247498	23.48286105605559
100	21.775864590555233	19.038241043727176	23.668211804450756	35.51768256126683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1558.0
1	1105.5
2	633.5
3	570.5
4	482.0
5	374.0
6	310.0
7	311.0
8	325.5
9	331.5
10	290.5
11	260.0
12	262.5
13	262.0
14	289.0
15	346.0
16	415.0
17	485.0
18	583.0
19	778.0
20	1037.0
21	1430.0
22	2215.5
23	3236.5
24	4193.0
25	5564.0
26	7882.0
27	10440.5
28	12914.0
29	16059.0
30	19984.0
31	23377.5
32	27625.0
33	32270.5
34	36093.5
35	39804.0
36	43482.0
37	48724.5
38	51985.5
39	52746.5
40	54067.5
41	54247.5
42	54140.5
43	55896.5
44	60268.0
45	67732.0
46	87652.0
47	266961.0
48	673014.0
49	693230.5
50	338816.0
51	158188.0
52	92816.5
53	71188.5
54	63392.5
55	56100.0
56	49844.5
57	43832.5
58	38267.5
59	33913.0
60	27579.5
61	21430.5
62	17050.5
63	13416.5
64	9946.0
65	6955.0
66	5199.0
67	4016.5
68	3010.0
69	2187.0
70	1782.5
71	1523.5
72	1249.5
73	1077.0
74	903.0
75	762.5
76	588.5
77	412.5
78	287.5
79	179.5
80	110.0
81	76.5
82	65.5
83	43.0
84	27.5
85	22.5
86	20.5
87	19.0
88	13.0
89	11.0
90	8.0
91	6.0
92	5.5
93	4.5
94	6.5
95	8.5
96	6.0
97	5.0
98	7.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0606328134853181
2	0.008761483402848543
3	0.010603069086249828
4	0.00892890028315775
5	0.011021611287022849
6	0.01127273660748666
7	0.0030135038455657405
8	0.005329437356509782
9	0.006780383652522916
10-11	0.006626918178906142
12-13	0.006027007691131481
14-15	0.009919450158320563
16-17	0.009500907957547544
18-19	0.0075616624272992206
20-21	0.006375792858442331
22-23	0.0024414961711759476
24-25	0.0036552685534177037
26-27	0.0047992839021972905
28-29	0.005999104877746613
30-31	0.008454552455614996
32-33	0.009877595938243262
34-35	0.011258785200794225
36-37	0.017662480872621428
38-39	0.018011266039932274
40-41	0.01799731463323984
42-43	0.02380109981729238
44-45	0.0278749105714831
46-47	0.03056753206312286
48-49	0.03207428398590573
50-51	0.03224170086621494
52-53	0.03077680316350937
54-55	0.03687356788810302
56-57	0.03970570344666712
58-59	0.037166547428644135
60-61	0.03779436072980367
62-63	0.03293927120083664
64-65	0.03554818425232179
66-67	0.03782226354318853
68-69	0.040249808307672046
70-71	0.040305613934441785
72-73	0.03617599755348132
74-75	0.03962199500651251
76-77	0.03871515357150431
78-79	0.03824080574396155
80-81	0.04320750652646805
82-83	0.038561688097887535
84-85	0.03511569064485634
86-87	0.033469424655149134
88-89	0.03098607426389588
90-91	0.033469424655149134
92-93	0.03529705893185798
94-95	0.038882570451813515
96-97	0.03404143232953893
98-99	0.03127905380443699
100	0.02834925839902586
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3583868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.005486196692175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60380431003865	40.60421891969863
2	13.817388184704235	14.64794757397356
3	4.727703871931882	7.517827269772005
4	2.046527341199926	4.339087069405032
5	1.0673631504969285	2.8288051370261407
6	0.5839067692598674	1.8570157318895422
7	0.35217329538363873	1.3066981723110742
8	0.2150736106409909	0.9120065040083033
9	0.1428197790731588	0.6813208645449251
>10	0.4334475420003432	3.302124962364533
>50	0.006584721597620611	0.2303831042956527
>100	0.0016950020111862973	0.13660456794894604
>500	4.6536051126469866E-4	0.1841057847415209
>1k	1.7451019172318418E-4	0.24968826743087388
>5k	2.3268025563091225E-4	0.8598181063938442
>10k+	6.398707029850087E-4	20.342347964195344
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	271324	7.570702938835917	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	66130	1.8452130491413188	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	62402	1.7411913608425311	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	48697	1.3587833034029155	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	46018	1.284031666344854	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	43084	1.2021648118736517	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	41186	1.1492052720691723	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	35195	0.9820395170804281	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	23430	0.6537629176074565	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	11742	0.3276348347651197	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	10544	0.2942072643300479	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	8384	0.23393718741873304	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	6834	0.1906878266721877	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	6465	0.18039168853317142	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	6203	0.173081151426336	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	4253	0.11867066532584349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.580562676973594E-5
7	0.0	0.0	0.0	0.0	5.580562676973594E-5
8	0.0	0.0	0.0	0.0	5.580562676973594E-5
9	0.0	0.0	0.0	2.790281338486797E-5	5.580562676973594E-5
10-11	0.0	0.0	0.0	1.3951406692433986E-4	5.580562676973594E-5
12-13	0.0	0.0	0.0	2.0927110038650978E-4	1.813682870016418E-4
14-15	0.0	0.0	0.0	2.929795405411137E-4	3.4878516731084964E-4
16-17	0.0	0.0	0.0	7.115217413141332E-4	4.464450141578875E-4
18-19	0.0	0.0	0.0	0.0011021611287022849	5.022506409276235E-4
20-21	0.0	0.0	0.0	0.001981099750325626	6.138618944670953E-4
22-23	0.0	0.0	0.0	0.0033204347927992886	6.696675212368312E-4
24-25	0.0	0.0	0.0	0.004590012801810781	6.696675212368312E-4
26-27	0.0	0.0	0.0	0.00598515347105418	7.673273680838691E-4
28-29	0.0	0.0	0.0	0.010407749392555753	8.091815881611711E-4
30-31	0.0	0.0	0.0	0.019448260929252975	8.091815881611711E-4
32-33	0.0	0.0	0.0	0.045900128018107805	8.091815881611711E-4
34-35	0.0	0.0	0.0	0.07839295420478656	8.370844015460391E-4
36-37	0.0	0.0	0.0	0.11472241723188464	8.370844015460391E-4
38-39	0.0	0.0	0.0	0.16878411816506633	8.92890028315775E-4
40-41	0.0	0.0	0.0	0.2241851541407217	8.92890028315775E-4
42-43	0.0	0.0	0.0	0.28073020546515665	8.92890028315775E-4
44-45	0.0	0.0	0.0	0.35365420824650906	9.06841435008209E-4
46-47	0.0	0.0	0.0	0.4446871369146408	9.34744248393077E-4
48-49	0.0	0.0	0.0	0.5160067279263634	9.62647061777945E-4
50-51	0.0	0.0	0.0	0.5958645798338555	0.001088209722009851
52-53	0.0	0.0	0.0	0.6717323294273114	0.0011440153487795868
54-55	0.0	0.0	0.0	0.7689875854802688	0.0011440153487795868
56-57	0.0	0.0	0.0	0.9112500795230181	0.0011858695688568887
58-59	0.0	0.0	0.0	1.0225544021152564	0.0011998209755493226
60-61	0.0	0.0	0.0	1.1352538653767383	0.0012137723822417566
62-63	0.0	0.0	0.0	1.2463907710886675	0.0013114322290887946
64-65	0.0	0.0	0.0	1.3560209248778137	0.0013532864491660964
66-67	0.0	0.0	0.0	1.495325720701767	0.0013811892625509646
68-69	0.0	0.0	0.0	1.657287600994233	0.0013951406692433986
70-71	0.0	0.0	0.0	1.81380843267665	0.0014369948893207004
72-73	0.0	0.0	0.0	1.9397198780758664	0.0015625575495526061
74-75	0.0	0.0	0.0	2.0921668990040927	0.0016741688030920783
76-77	0.0	0.0	0.0	2.2288767331832533	0.0017020716164769462
78-79	0.0	0.0	0.0	2.475997999926337	0.001757877243246682
80-81	0.0	0.0	0.0	2.7121116067890894	0.001771828649939116
82-83	0.0	0.0	0.0	2.888206262060991	0.001869488496786154
84-85	0.0	0.0	0.0	3.075071403299452	0.001869488496786154
86-87	0.0	0.0	0.0	3.2552398693255444	0.00199505115701806
88	0.0	0.0	0.0	3.4176202918187837	0.0020927110038650976
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCA	4700	0.0	27.395405	3
TACGCAG	4720	0.0	27.378502	4
GAGTACG	4875	0.0	26.617767	1
CGCAGTG	4975	0.0	26.068905	6
GTACTAG	725	0.0	25.939352	1
AGTACGC	5045	0.0	25.428835	2
GTCGTAA	150	1.1604334E-6	25.074709	1
AGTAGGC	2530	0.0	22.47449	2
ACGCAGT	5880	0.0	22.137133	5
GCTTATG	2280	0.0	22.064095	1
TACCTGG	2530	0.0	21.731531	2
GTAGGCA	2775	0.0	20.828938	3
GTACCTG	2900	0.0	20.42724	1
TAGGCAG	2765	0.0	20.054222	4
CTAGGCA	1125	0.0	20.04969	4
GCATCGT	805	0.0	19.846218	8
GGGCTAT	1210	0.0	19.417418	6
GGCTATT	1180	0.0	19.113304	7
TTTAGGG	1280	0.0	19.090548	2
TATGTAC	2610	0.0	18.90461	4
>>END_MODULE