FastQCFastQC Report
Mon 16 Jan 2017
ERR1146554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583868
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2893838.074599845753246No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC728172.031799162245931No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT670091.8697396221066178No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC550441.5358824599566725No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA516651.4415988535292035No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA487511.3602900553256982No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG452831.2635230985069763No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC405821.132351972784712No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG258120.720227419090212No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT225060.6279807180398386No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA140020.39069519301492134No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129060.360113709545106No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG124990.34875726449746475No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89340.24928373478041044No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA77310.21571665027841427No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG69480.19386874739806265No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA69190.19305956580990147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63640.17757350438129976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.1690073406721453No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46270.12910631753178411No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC44740.1248371870838993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG52900.030.0577981
TACGCAG53300.029.45824
GTACGCA53400.029.4030343
GTACTAG7250.029.1991791
AGTACGC54650.028.9215152
CGCAGTG54950.028.7387356
ATAACGC1853.274181E-1027.9516813
ACGCAGT65100.024.190835
CGACGAG4250.023.2224624-25
GTACCCG2504.3291948E-1022.5806981
TAGTACT12750.022.4908894
TAGGCAT10550.022.2794515
GCTTATG21350.022.2545931
GTATAAC4000.021.1694051
GTATTAG4450.021.1429771
AGTAGGC23250.021.0417692
GAGTACT69350.020.6695612-13
GCATCGT9550.020.6692078
CTAGGCA12050.019.8961984
TAGGCAG24600.019.8739954
Produced by FastQC (version 0.11.5)