##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146554_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3583868
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53283045022864	33.0	31.0	34.0	31.0	34.0
2	32.47629488586075	34.0	31.0	34.0	31.0	34.0
3	32.68152147344713	34.0	31.0	34.0	31.0	34.0
4	35.99721892658993	37.0	35.0	37.0	35.0	37.0
5	36.06328553395382	37.0	35.0	37.0	35.0	37.0
6	36.26220831794028	37.0	37.0	37.0	35.0	37.0
7	36.292754364837094	37.0	37.0	37.0	35.0	37.0
8	36.308747420384904	37.0	37.0	37.0	35.0	37.0
9	38.09180388340196	39.0	38.0	39.0	37.0	39.0
10-11	38.06806793665392	39.0	38.0	39.0	36.0	39.0
12-13	38.08578482801264	39.0	38.0	39.0	37.0	39.0
14-15	39.319254224764975	40.0	39.0	41.0	36.5	41.0
16-17	39.47643635312461	40.5	39.0	41.0	37.0	41.0
18-19	39.59074050718386	41.0	39.5	41.0	37.0	41.0
20-21	39.60125596143608	41.0	39.5	41.0	37.0	41.0
22-23	39.5319616961339	41.0	39.0	41.0	37.0	41.0
24-25	39.455400143085626	41.0	39.0	41.0	36.5	41.0
26-27	39.26123534683755	40.5	39.0	41.0	36.0	41.0
28-29	39.26251887625325	40.0	39.0	41.0	36.0	41.0
30-31	39.34319302496632	41.0	39.0	41.0	36.0	41.0
32-33	39.19307407527286	41.0	39.0	41.0	35.0	41.0
34-35	38.99669309807169	40.0	38.5	41.0	35.0	41.0
36-37	38.85879823140807	40.0	38.5	41.0	35.0	41.0
38-39	38.75968060207575	40.0	38.0	41.0	35.0	41.0
40-41	38.92001016778519	40.0	38.5	41.0	35.0	41.0
42-43	38.85427867321006	40.0	38.0	41.0	35.0	41.0
44-45	38.71281573428486	40.0	38.0	41.0	35.0	41.0
46-47	38.50327439515071	40.0	38.0	41.0	34.5	41.0
48-49	38.26900097883069	40.0	38.0	41.0	34.0	41.0
50-51	37.995224433489184	40.0	37.5	41.0	33.5	41.0
52-53	38.1473968907337	40.0	38.0	41.0	34.0	41.0
54-55	38.060189856322836	40.0	38.0	41.0	33.5	41.0
56-57	37.685992899292046	40.0	37.0	41.0	32.5	41.0
58-59	37.455525705745856	39.0	36.0	41.0	32.0	41.0
60-61	36.9230425060298	39.0	35.5	41.0	31.0	41.0
62-63	36.902219892027276	39.0	35.0	40.5	31.5	41.0
64-65	36.61921700241192	38.5	35.0	39.5	31.5	41.0
66-67	36.11944957236148	37.0	35.0	39.0	31.0	41.0
68-69	35.50276558734864	37.0	35.0	39.0	30.0	41.0
70-71	34.86391546786879	36.0	34.0	38.0	29.5	40.0
72-73	34.407159248052665	35.0	34.0	37.0	29.5	39.0
74-75	34.235081481795646	35.0	34.0	37.0	29.0	39.0
76-77	33.99593553668829	35.0	34.0	36.5	29.0	39.0
78-79	33.650355286522824	35.0	34.0	36.0	29.0	37.0
80-81	33.24960824450008	35.0	33.0	35.5	28.5	37.0
82-83	32.92905081883596	35.0	32.5	35.0	27.5	36.5
84-85	32.801446509748686	35.0	33.0	35.0	28.0	36.0
86-87	32.97581328330173	35.0	33.5	35.0	29.0	36.0
88-89	32.79668335998982	35.0	33.0	35.0	28.0	36.0
90-91	32.57276272451999	35.0	33.0	35.0	27.0	35.0
92-93	32.42764828392117	35.0	33.0	35.0	26.5	35.0
94-95	32.28310710662335	35.0	32.5	35.0	26.0	35.0
96-97	32.179091277915376	35.0	32.0	35.0	25.0	35.0
98-99	32.388192310654304	35.0	33.0	35.0	27.0	35.0
100	31.588122944260224	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2645705498515838
1101	2	0.27221574062016174
1101	3	0.28459733779421015
1101	4	0.2091946699635372
1101	5	0.20088647981874175
1101	6	0.15797302640827127
1101	7	0.2126752265239773
1101	8	0.18436521274089301
1101	9	0.2121704530636066
1101	10-11	0.23516329270903924
1101	12-13	0.2194256794159415
1101	14-15	0.40224073139027894
1101	16-17	0.3317698428030127
1101	18-19	0.33036857462828095
1101	20-21	0.28443612365347093
1101	22-23	0.3378378707254299
1101	24-25	0.3335845692681687
1101	26-27	0.36621883467344674
1101	28-29	0.3991692130648872
1101	30-31	0.3908054255158362
1101	32-33	0.3842102511324086
1101	34-35	0.4022460112702362
1101	36-37	0.4528808701485829
1101	38-39	0.47357065658736275
1101	40-41	0.4572468341920626
1101	42-43	0.4498589963086559
1101	44-45	0.45873804574953425
1101	46-47	0.5011755837698644
1101	48-49	0.4941688513417475
1101	50-51	0.44141680550999496
1101	52-53	0.4499911752761392
1101	54-55	0.44475486951234444
1101	56-57	0.4764927659247391
1101	58-59	0.5061100674995913
1101	60-61	0.5251130082470183
1101	62-63	0.5643551560374291
1101	64-65	0.4798835977523055
1101	66-67	0.4854245693696484
1101	68-69	0.49645517111812154
1101	70-71	0.49972535466347523
1101	72-73	0.5014883977405731
1101	74-75	0.5019436476668844
1101	76-77	0.45180447580668215
1101	78-79	0.4430432695814943
1101	80-81	0.5222024957198528
1101	82-83	0.4977869058851212
1101	84-85	0.5107541233007282
1101	86-87	0.4591153949302793
1101	88-89	0.4339500808160679
1101	90-91	0.4548416299521847
1101	92-93	0.3893403274193403
1101	94-95	0.48205826170082133
1101	96-97	0.48329033710008673
1101	98-99	0.5376545897696516
1101	100	0.5563146264779952
1102	1	0.25139078961599637
1102	2	0.20513251530049104
1102	3	0.237128705092978
1102	4	0.14735421030542284
1102	5	0.12447473944612852
1102	6	0.159485905779988
1102	7	0.18511076993026876
1102	8	0.16959105228485072
1102	9	0.20849424052224208
1102	10-11	0.20354574337860498
1102	12-13	0.23751513818163517
1102	14-15	0.2827735746732074
1102	16-17	0.23335344678174863
1102	18-19	0.24362272703063326
1102	20-21	0.2604570690651613
1102	22-23	0.2707802085167259
1102	24-25	0.2947524028630326
1102	26-27	0.29395690519969975
1102	28-29	0.31322866934718263
1102	30-31	0.30081387539777893
1102	32-33	0.269773976146773
1102	34-35	0.3678487591201929
1102	36-37	0.35954610873629633
1102	38-39	0.3476723570086122
1102	40-41	0.3837800125186561
1102	42-43	0.3403313437427826
1102	44-45	0.34447258680955883
1102	46-47	0.3248181847733633
1102	48-49	0.3518621091195442
1102	50-51	0.3486343302787631
1102	52-53	0.43105754088959003
1102	54-55	0.37158485664030394
1102	56-57	0.44462896301755706
1102	58-59	0.48411573372565897
1102	60-61	0.5183066762205115
1102	62-63	0.4636334242043034
1102	64-65	0.5281322127982975
1102	66-67	0.42187619083075134
1102	68-69	0.3710644222762127
1102	70-71	0.3185462566090891
1102	72-73	0.3951406073113475
1102	74-75	0.38071217510255906
1102	76-77	0.40514492435231375
1102	78-79	0.33512982645093814
1102	80-81	0.39503737269320993
1102	82-83	0.3816392640733284
1102	84-85	0.3922598581782353
1102	86-87	0.419279568959368
1102	88-89	0.38725033993465985
1102	90-91	0.40714760703797026
1102	92-93	0.48657858026943046
1102	94-95	0.34578144887435514
1102	96-97	0.2776490548368713
1102	98-99	0.4041523188254459
1102	100	0.43444100153837795
1103	1	0.17927355674018486
1103	2	0.14624984155526732
1103	3	0.17526109886317442
1103	4	0.14130465158474692
1103	5	0.08771286666369349
1103	6	0.0453695478086118
1103	7	0.08216923729855097
1103	8	0.10144611689398886
1103	9	0.1620835525454254
1103	10-11	0.19903449855141986
1103	12-13	0.16135949136344152
1103	14-15	0.26348386617144826
1103	16-17	0.19660204297804995
1103	18-19	0.15314463230014752
1103	20-21	0.18012259447643686
1103	22-23	0.19972580363024406
1103	24-25	0.17758980952129377
1103	26-27	0.26005440191362794
1103	28-29	0.2498853472410545
1103	30-31	0.2725741182573529
1103	32-33	0.302179221204419
1103	34-35	0.3066930218586563
1103	36-37	0.320043867446671
1103	38-39	0.2584739600186339
1103	40-41	0.2620344456562691
1103	42-43	0.21362043740249703
1103	44-45	0.29062041923664594
1103	46-47	0.3900257405871628
1103	48-49	0.3812806732652234
1103	50-51	0.3943140029282617
1103	52-53	0.3762830886686501
1103	54-55	0.40195782469248087
1103	56-57	0.45716898635496506
1103	58-59	0.42227731112316036
1103	60-61	0.3584591641268915
1103	62-63	0.4228590616927974
1103	64-65	0.3202750866853563
1103	66-67	0.3528506524370769
1103	68-69	0.4564037378502164
1103	70-71	0.41911113679886824
1103	72-73	0.3262143623242082
1103	74-75	0.3423102126849429
1103	76-77	0.3184387918663276
1103	78-79	0.3440873652661338
1103	80-81	0.4026590698221497
1103	82-83	0.32398359780658126
1103	84-85	0.37226428148363766
1103	86-87	0.22340519273242876
1103	88-89	0.29417380151175365
1103	90-91	0.2693816318405098
1103	92-93	0.3022856790529431
1103	94-95	0.28699405362836217
1103	96-97	0.2648494655834739
1103	98-99	0.2978029069683572
1103	100	0.40586569744045065
1104	1	0.16226432814441694
1104	2	0.2174145418722162
1104	3	0.1823390360599504
1104	4	0.1763672673404173
1104	5	0.11091613426103208
1104	6	0.12967293006649783
1104	7	0.12566444317268122
1104	8	0.09677071064668752
1104	9	0.15973822856417286
1104	10-11	0.15239153484072432
1104	12-13	0.16546959977813458
1104	14-15	0.2735281160645897
1104	16-17	0.23312133411781133
1104	18-19	0.21886733278115855
1104	20-21	0.21709529632192215
1104	22-23	0.20884281549076178
1104	24-25	0.2078975963518772
1104	26-27	0.22308461095273913
1104	28-29	0.27026004358424416
1104	30-31	0.24691157767527727
1104	32-33	0.2618046857684604
1104	34-35	0.2695937571764091
1104	36-37	0.31181847571134114
1104	38-39	0.29001628535211665
1104	40-41	0.2590399337653224
1104	42-43	0.2666408258446893
1104	44-45	0.20781678941206394
1104	46-47	0.26416566009876874
1104	48-49	0.3255785919544323
1104	50-51	0.28320662924922857
1104	52-53	0.28040944547946367
1104	54-55	0.26082291768371846
1104	56-57	0.33340167017964717
1104	58-59	0.33045070718228686
1104	60-61	0.3674141771735222
1104	62-63	0.32891141338635776
1104	64-65	0.3337984703267267
1104	66-67	0.3344086823972745
1104	68-69	0.381091188691137
1104	70-71	0.3513586400987734
1104	72-73	0.3234869847389348
1104	74-75	0.2734390872896526
1104	76-77	0.25424879341515805
1104	78-79	0.29302199969480114
1104	80-81	0.3188874495805223
1104	82-83	0.3358491315946992
1104	84-85	0.3441686052348132
1104	86-87	0.2998761526762692
1104	88-89	0.32065033862964043
1104	90-91	0.27215006502736827
1104	92-93	0.36295316348951445
1104	94-95	0.3597453073402974
1104	96-97	0.26176130002335896
1104	98-99	0.2792557610078319
1104	100	0.3583721085627083
1105	1	0.14772101371469404
1105	2	0.12184789119526584
1105	3	0.12767499154674056
1105	4	0.07931444182604963
1105	5	0.04752729919535881
1105	6	0.09591492578656613
1105	7	0.07449822986409771
1105	8	0.0748819619194876
1105	9	0.13116143098302757
1105	10-11	0.04625424379592147
1105	12-13	0.08482903947815856
1105	14-15	0.1429524893319254
1105	16-17	0.15065469274115628
1105	18-19	0.11957425613790917
1105	20-21	0.12098669016626928
1105	22-23	0.11797550850666738
1105	24-25	0.10486049656962138
1105	26-27	0.16569689851181124
1105	28-29	0.18754254889446997
1105	30-31	0.1896086686304983
1105	32-33	0.19271812954414003
1105	34-35	0.24998080961104563
1105	36-37	0.22934498266956638
1105	38-39	0.2183948938395801
1105	40-41	0.21981761423032253
1105	42-43	0.23152976746685283
1105	44-45	0.28642285446110094
1105	46-47	0.2961289025253677
1105	48-49	0.23968634266284994
1105	50-51	0.24439295724944543
1105	52-53	0.24510530493212457
1105	54-55	0.1827647799198715
1105	56-57	0.23593280257343707
1105	58-59	0.23522392530381353
1105	60-61	0.24463331169514646
1105	62-63	0.2749526785317258
1105	64-65	0.24949968246491494
1105	66-67	0.2776118043903111
1105	68-69	0.28102258218203957
1105	70-71	0.23311831318973475
1105	72-73	0.21364730471693605
1105	74-75	0.2010600813307306
1105	76-77	0.21242051980563303
1105	78-79	0.2048464610827878
1105	80-81	0.2257606843407416
1105	82-83	0.28941264518248033
1105	84-85	0.2818729664432169
1105	86-87	0.25356295879541335
1105	88-89	0.26458430490207263
1105	90-91	0.3357336483778326
1105	92-93	0.31359466654865287
1105	94-95	0.3014235407159518
1105	96-97	0.2934811398546415
1105	98-99	0.2550140643648149
1105	100	0.40494223988554623
1106	1	0.11198822921713258
1106	2	0.0855405605032118
1106	3	0.1083688375507279
1106	4	0.0684425045669812
1106	5	0.061327102472361616
1106	6	0.05683198273420231
1106	7	0.08303238338723418
1106	8	0.08247332784362982
1106	9	0.07003068418425329
1106	10-11	0.08415965980609741
1106	12-13	0.09067974938341905
1106	14-15	0.14289879950391793
1106	16-17	0.12739182242947322
1106	18-19	0.12692112740111838
1106	20-21	0.12458109046895771
1106	22-23	0.17885160799196598
1106	24-25	0.16530722195258107
1106	26-27	0.14806929231506416
1106	28-29	0.1449428137161135
1106	30-31	0.18212193644923502
1106	32-33	0.1721529382421565
1106	34-35	0.1985664764647197
1106	36-37	0.23797733871796112
1106	38-39	0.32566001637813
1106	40-41	0.24803169111068968
1106	42-43	0.246387216999139
1106	44-45	0.30684504149709824
1106	46-47	0.34979228305660826
1106	48-49	0.32713001892604865
1106	50-51	0.35319268392991887
1106	52-53	0.3591181115819708
1106	54-55	0.3624235178346069
1106	56-57	0.3956882226446723
1106	58-59	0.3858557960481903
1106	60-61	0.3606872350702943
1106	62-63	0.3663826258072973
1106	64-65	0.37580059494629836
1106	66-67	0.38804531325975944
1106	68-69	0.3991718540670419
1106	70-71	0.342068306353859
1106	72-73	0.3225308540091092
1106	74-75	0.346415716103472
1106	76-77	0.2934755762312591
1106	78-79	0.28573564267638574
1106	80-81	0.29302504235924687
1106	82-83	0.3956665852065342
1106	84-85	0.42140155674632496
1106	86-87	0.42916002147767074
1106	88-89	0.3794059274939414
1106	90-91	0.38636984032236654
1106	92-93	0.35902370391532656
1106	94-95	0.34184132272917367
1106	96-97	0.2924058258161111
1106	98-99	0.2967870990352921
1106	100	0.35870930031615345
1107	1	0.09960922426220264
1107	2	0.09715751460880284
1107	3	0.10264267948844719
1107	4	0.12529702498390805
1107	5	0.09677527095751515
1107	6	0.10139275143459514
1107	7	0.0870278687000976
1107	8	0.05175964833733815
1107	9	0.05066742237615074
1107	10-11	0.06176297253994534
1107	12-13	0.07573119688524343
1107	14-15	0.15013220067705646
1107	16-17	0.11805531242603706
1107	18-19	0.12571018997613947
1107	20-21	0.07234032806812962
1107	22-23	0.12023968649494066
1107	24-25	0.1397477889218237
1107	26-27	0.11894976300683169
1107	28-29	0.15364285867946137
1107	30-31	0.16073025575686017
1107	32-33	0.12158170953492942
1107	34-35	0.14174647347284974
1107	36-37	0.13739665639756282
1107	38-39	0.1813908905769992
1107	40-41	0.15234661465272836
1107	42-43	0.15832062173605266
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2216	66-67	-1.2492235227614188
2216	68-69	-1.3390949116519266
2216	70-71	-1.3693710136190234
2216	72-73	-1.3643704525971714
2216	74-75	-1.2899861136675455
2216	76-77	-1.168644898939874
2216	78-79	-1.2966164687809965
2216	80-81	-1.4649052425048446
2216	82-83	-1.3178974355316413
2216	84-85	-1.3761841629981362
2216	86-87	-1.4035364434783126
2216	88-89	-1.3856407972353892
2216	90-91	-1.4414862628367437
2216	92-93	-1.5479972248927965
2216	94-95	-1.669693644830513
2216	96-97	-1.4447552626737306
2216	98-99	-1.4681347863511007
2216	100	-1.7851887352092142
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	19.0
8	53.0
9	97.0
10	120.0
11	146.0
12	128.0
13	167.0
14	254.0
15	368.0
16	705.0
17	1352.0
18	2528.0
19	3942.0
20	5795.0
21	8600.0
22	12447.0
23	15999.0
24	17882.0
25	18825.0
26	19827.0
27	23525.0
28	28915.0
29	36078.0
30	45298.0
31	57109.0
32	73921.0
33	99455.0
34	145362.0
35	240226.0
36	492901.0
37	989550.0
38	1018884.0
39	222638.0
40	750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.94228952762047	17.185981390559665	13.03433775199412	18.83739132982575
2	13.196386511863532	27.688849940488257	31.282454285431825	27.83230926221638
3	31.978003549812755	21.92233996335227	29.5032354860735	16.59642100076147
4	10.701342794991334	14.8740969254448	45.09577361666222	29.328786662901646
5	10.4015082449346	43.52732019108753	33.9741997218657	12.096971842112168
6	26.569771562541682	38.25907474262906	14.932118363563408	20.239035331265846
7	24.889686165721596	33.31463843793267	22.998209196437593	18.797466199908143
8	23.40725160636497	22.923974878539052	19.9160236928369	33.75274982225908
9	19.032062564804285	24.52406171209431	27.509300007701178	28.934575715400236
10-11	27.39789802526209	21.269617072950233	26.864898483984344	24.467586417803332
12-13	35.02157445530918	21.251396535809914	23.603310724613742	20.123718284267166
14-15	24.29028476782202	24.90103566901339	32.66173102963921	18.14694853352538
16-17	19.47460118508829	27.38283050603426	30.774473278591735	22.368095030285716
18-19	18.158704505857916	35.9708142152557	25.367396343838557	20.50308493504783
20-21	23.444027514406223	25.198877302400646	22.939460939967653	28.417634243225475
22-23	19.94853856844973	26.719415354832194	25.919828095435943	27.412217981282133
24-25	28.102653334330398	26.13504459427635	23.447194483725404	22.31510758766785
26-27	24.964437864340987	20.100210163990415	34.327003673126356	20.608348298542246
28-29	18.935395166949647	35.072540399819744	25.87861149903805	20.113452934192555
30-31	22.56730266040881	24.305188265243697	25.263315472269966	27.86419360207753
32-33	24.090274228834733	26.01481634019181	27.85077962343744	22.044129807536024
34-35	28.378655524744257	23.363074393761742	27.101527363803225	21.156742717690776
36-37	24.554056929199497	21.225013689934325	33.824413076764785	20.3965163041014
38-39	21.776319690388178	28.13416047161671	30.253324469476546	19.836195368518567
40-41	21.46806139758914	29.9917420552335	26.402068294975074	22.138128252202293
42-43	20.053116180903064	29.435240169628013	21.41485311015385	29.09679053931507
44-45	24.148132460472286	22.672580982472322	24.283322402340612	28.895964154714775
46-47	28.84400800589979	28.063717386045255	25.805633427896257	17.286641180158696
48-49	25.687564612866908	26.180359362639848	26.913873228334374	21.218202796158874
50-51	15.828521801002418	28.338756988842267	34.77331212701469	21.05940908314063
52-53	22.616823003617093	31.637019057045013	22.78964144115537	22.956516498182523
54-55	19.404118486535236	24.77138547690709	27.003377544581316	28.82111849197636
56-57	26.42013373941602	21.31946643748945	26.651840835306313	25.60855898778822
58-59	32.86268089817913	22.655411152011393	23.67723612807022	20.804671821739255
60-61	23.87742790406052	25.867786743739394	33.09666189612649	17.158123456073593
62-63	19.784808461226053	26.87555954445948	30.561824840767443	22.777807153547023
64-65	18.374345720668558	35.546893530008255	24.485394202773993	21.593366546549195
66-67	23.540353406664057	25.30511255278561	22.146856679730845	29.007677360819496
68-69	20.275580087280094	26.713251023822952	25.523700805539	27.487468083357946
70-71	28.00391762113339	26.079079303227086	23.052507703050455	22.86449537258907
72-73	25.002305582215666	20.604342977433756	33.287779772461015	21.105571667889567
74-75	19.508698303032162	34.729382252364495	24.914300543146904	20.84761890145644
76-77	22.539925435501374	24.514034628041127	24.68235026897855	28.263689667478946
78-79	24.23091931686134	26.138649306519078	27.037377919022905	22.593053457596675
80-81	28.199101194073663	23.547908878289533	26.21971636216309	22.03327356547371
82-83	24.20886638863315	21.74085737991197	32.73687689573855	21.313399335716333
84-85	21.582281244807454	28.41317756661212	29.111013854182783	20.89352733439764
86-87	21.519196789501844	30.12163535621783	25.594612524040024	22.764555330240306
88-89	20.041745468994062	29.528522582621765	21.056000482161423	29.37373146622275
90-91	23.95014269498765	23.02473193767181	23.828374817376087	29.196750549964452
92-93	28.319430291517435	28.112391416201714	25.19116217449973	18.37701611778112
94-95	24.819011863580336	26.357098860379185	26.636936214857275	22.186953061183203
96-97	16.239137732009507	28.102479706885138	33.97734727252891	21.68103528857645
98-99	22.732660536942415	31.05031240164203	22.870501204292147	23.346525857123403
100	21.97172817731541	19.34728871261668	23.478459344431453	35.202523765636464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	370.0
1	243.5
2	336.5
3	570.5
4	362.5
5	187.5
6	389.5
7	967.0
8	1808.0
9	2260.5
10	1682.0
11	977.5
12	935.0
13	1067.5
14	1238.5
15	1417.5
16	1628.0
17	1865.5
18	2095.5
19	2376.0
20	2752.0
21	3262.0
22	4089.5
23	5056.5
24	5970.0
25	7292.0
26	9499.5
27	11984.5
28	14320.0
29	17118.5
30	20537.5
31	23624.5
32	27493.0
33	31707.0
34	34928.0
35	38408.0
36	42697.5
37	47673.5
38	50339.5
39	51490.0
40	52694.0
41	52653.5
42	52803.5
43	54499.5
44	58182.0
45	64701.0
46	81369.0
47	277431.0
48	713025.5
49	712932.0
50	323438.0
51	140146.5
52	79499.0
53	62067.5
54	57757.0
55	52598.0
56	47606.0
57	42875.0
58	37599.0
59	33318.0
60	27029.5
61	20511.0
62	16224.0
63	12771.5
64	9365.5
65	6570.5
66	5005.5
67	3833.5
68	2757.0
69	2054.0
70	1598.0
71	1302.5
72	1066.5
73	940.0
74	784.0
75	663.5
76	518.0
77	329.0
78	213.0
79	116.5
80	67.0
81	48.0
82	36.5
83	19.0
84	7.0
85	1.5
86	2.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005664271117128198
2	0.08543841458446572
3	4.743478275427555E-4
4	0.0
5	0.039789411886821724
6	0.003627365740032836
7	5.5805626769735944E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.7902813384867972E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.301534543124914E-4
24-25	0.0
26-27	0.0
28-29	8.37084401546039E-5
30-31	4.185422007730195E-5
32-33	0.0015207033294753042
34-35	0.0012835294157039266
36-37	8.510358082384731E-4
38-39	7.115217413141332E-4
40-41	0.0012974808223963606
42-43	7.254731480065672E-4
44-45	5.999104877746613E-4
46-47	3.069309472335477E-4
48-49	6.417647078519633E-4
50-51	8.510358082384731E-4
52-53	0.0013672378558585306
54-55	0.0014369948893207004
56-57	9.207928417006429E-4
58-59	0.0023438363243289094
60-61	0.001869488496786154
62-63	0.0010463555019325488
64-65	0.004297033261269667
66-67	0.004171470601037761
68-69	0.0033064833861068546
70-71	0.0016881202097845122
72-73	0.00486904093565946
74-75	0.0034320460463387603
76-77	0.003487851673108496
78-79	7.673273680838692E-4
80-81	5.5805626769735944E-5
82-83	2.0927110038650978E-4
84-85	1.2556266023190586E-4
86-87	1.3951406692433986E-5
88-89	1.674168803092078E-4
90-91	0.0
92-93	0.0
94-95	1.3951406692433986E-5
96-97	4.743478275427555E-4
98-99	5.580562676973594E-4
100	4.743478275427555E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3583868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.20197778967567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41214948350942	35.86812776686147
2	14.921381577282258	14.384802067588701
3	5.120528236722839	7.404587650137641
4	2.3210293598331377	4.475128226074481
5	1.1978657983177903	2.8869750302763117
6	0.6656982840433392	1.9252784341249343
7	0.4076983473735597	1.375630667949146
8	0.24153005380717416	0.9313781031322064
9	0.17156163938632724	0.7442649298134083
>10	0.521111870432656	3.7377108337535785
>50	0.011939347605214588	0.3891092325503162
>100	0.004840305525348052	0.42669204216468787
>500	9.520343432110714E-4	0.32171906053785737
>1k	5.077516496990211E-4	0.6168831434535849
>5k	3.808137372742658E-4	1.3140736788713465
>10k+	8.250964307609092E-4	23.197639132710364
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	289383	8.074599845753246	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	72817	2.031799162245931	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	67009	1.8697396221066178	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	55044	1.5358824599566725	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	51665	1.4415988535292035	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	48751	1.3602900553256982	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	45283	1.2635230985069763	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	40582	1.132351972784712	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	25812	0.720227419090212	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	22506	0.6279807180398386	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	14002	0.39069519301492134	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	12906	0.360113709545106	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	12499	0.34875726449746475	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	8934	0.24928373478041044	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	7731	0.21571665027841427	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	6948	0.19386874739806265	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	6919	0.19305956580990147	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6364	0.17757350438129976	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6057	0.1690073406721453	No Hit
GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4627	0.12910631753178411	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	4474	0.1248371870838993	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.790281338486797E-5
2	0.0	0.0	0.0	0.0	2.790281338486797E-5
3	0.0	0.0	0.0	0.0	2.790281338486797E-5
4	0.0	0.0	0.0	0.0	2.790281338486797E-5
5	0.0	0.0	0.0	0.0	2.790281338486797E-5
6	0.0	0.0	0.0	0.0	2.790281338486797E-5
7	0.0	0.0	0.0	0.0	2.790281338486797E-5
8	0.0	0.0	0.0	0.0	2.790281338486797E-5
9	0.0	0.0	0.0	5.580562676973594E-5	2.790281338486797E-5
10-11	0.0	0.0	0.0	1.6741688030920784E-4	5.580562676973594E-5
12-13	0.0	0.0	0.0	2.3717391377137774E-4	3.7668798069571757E-4
14-15	0.0	0.0	0.0	3.2088235392598166E-4	4.882992342351894E-4
16-17	0.0	0.0	0.0	7.394245546990012E-4	5.301534543124914E-4
18-19	0.0	0.0	0.0	0.0011021611287022849	6.696675212368312E-4
20-21	0.0	0.0	0.0	0.001981099750325626	7.533759613914351E-4
22-23	0.0	0.0	0.0	0.0033343861994917226	8.091815881611711E-4
24-25	0.0	0.0	0.0	0.004645818428580517	8.091815881611711E-4
26-27	0.0	0.0	0.0	0.005999104877746613	8.649872149309071E-4
28-29	0.0	0.0	0.0	0.010421700799248187	8.92890028315775E-4
30-31	0.0	0.0	0.0	0.01943430952256054	9.06841435008209E-4
32-33	0.0	0.0	0.0	0.04581641957795321	9.76598468470379E-4
34-35	0.0	0.0	0.0	0.07812787747763031	0.001004501281855247
36-37	0.0	0.0	0.0	0.11438758347126624	0.001004501281855247
38-39	0.0	0.0	0.0	0.16844928440444792	0.0010463555019325488
40-41	0.0	0.0	0.0	0.22364104927971679	0.0010603069086249828
42-43	0.0	0.0	0.0	0.28011634357068954	0.0010603069086249828
44-45	0.0	0.0	0.0	0.35287292947173277	0.0011440153487795868
46-47	0.0	0.0	0.0	0.44391980954655696	0.0012416751956266245
48-49	0.0	0.0	0.0	0.51564399135236	0.0013811892625509646
50-51	0.0	0.0	0.0	0.5955576488866219	0.0014230434826282665
52-53	0.0	0.0	0.0	0.6714393498867703	0.0015904603629374743
54-55	0.0	0.0	0.0	0.7685829946861882	0.0016741688030920783
56-57	0.0	0.0	0.0	0.9107199260687057	0.0016881202097845122
58-59	0.0	0.0	0.0	1.0219963458475592	0.0017020716164769462
60-61	0.0	0.0	0.0	1.1341098500279587	0.0017020716164769462
62-63	0.0	0.0	0.0	1.2453723184001197	0.001757877243246682
64-65	0.0	0.0	0.0	1.3545420757684155	0.00178578005663155
66-67	0.0	0.0	0.0	1.4937073575254445	0.00178578005663155
68-69	0.0	0.0	0.0	1.6558506061049125	0.00178578005663155
70-71	0.0	0.0	0.0	1.8125388546676384	0.00178578005663155
72-73	0.0	0.0	0.0	1.938966502114475	0.0019252941235558898
74-75	0.0	0.0	0.0	2.09174835680332	0.0021066624105575316
76-77	0.0	0.0	0.0	2.228360531135633	0.0021485166306348335
78-79	0.0	0.0	0.0	2.476290979466878	0.0022601278841743056
80-81	0.0	0.0	0.0	2.7131021566642524	0.0022880306975591735
82-83	0.0	0.0	0.0	2.8899780907109305	0.0023438363243289094
84-85	0.0	0.0	0.0	3.0776942677576296	0.0023717391377137773
86-87	0.0	0.0	0.0	3.258546352711651	0.002455447577868381
88	0.0	0.0	0.0	3.421275560372201	0.0025391560180229853
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	5290	0.0	30.057798	1
TACGCAG	5330	0.0	29.4582	4
GTACGCA	5340	0.0	29.403034	3
GTACTAG	725	0.0	29.199179	1
AGTACGC	5465	0.0	28.921515	2
CGCAGTG	5495	0.0	28.738735	6
ATAACGC	185	3.274181E-10	27.951681	3
ACGCAGT	6510	0.0	24.19083	5
CGACGAG	425	0.0	23.22246	24-25
GTACCCG	250	4.3291948E-10	22.580698	1
TAGTACT	1275	0.0	22.490889	4
TAGGCAT	1055	0.0	22.279451	5
GCTTATG	2135	0.0	22.254593	1
GTATAAC	400	0.0	21.169405	1
GTATTAG	445	0.0	21.142977	1
AGTAGGC	2325	0.0	21.041769	2
GAGTACT	6935	0.0	20.66956	12-13
GCATCGT	955	0.0	20.669207	8
CTAGGCA	1205	0.0	19.896198	4
TAGGCAG	2460	0.0	19.873995	4
>>END_MODULE