FastQCFastQC Report
Mon 16 Jan 2017
ERR1146553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4129436
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT973982.3586271829857637No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC238520.5776091456557264No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT237450.5750179927718942No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA167190.4048736921942851No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA155780.3772428002274402No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC128820.31195543410770865No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA91090.22058702447501308No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG83240.20157716453288052No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG77960.18879091478836335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13100.021.8917081
CATGGGG68050.019.4745984
CTAACGC6300.019.3945793
GTATAAG16950.019.1381761
TAACGCC6950.018.9330584
CGCCTAC6050.018.6408887
TACCTGG34950.018.5557922
TAGTACT19600.018.4621474
GTACCTG37350.017.8738821
GAGTACG17300.017.6639731
CTAGTAC17600.017.622963
ATGGGGG44000.017.3025425
GTACGCA17100.016.764143
GCTATTG19100.016.729838
GTCTAAC12450.016.615161
TACGGGC9100.016.5255584
GGCGTCG8250.016.5180998
GTATAAT17100.016.495911
GGGCTAT22250.016.4738966
TCTAACG6900.016.3459322
Produced by FastQC (version 0.11.5)