##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146553_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4129436
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.475725498591093	31.0	31.0	34.0	29.0	34.0
2	31.640039220852437	33.0	31.0	34.0	29.0	34.0
3	31.71610844677094	34.0	31.0	34.0	30.0	34.0
4	35.20739732980484	37.0	35.0	37.0	33.0	37.0
5	35.28739856968361	37.0	35.0	37.0	33.0	37.0
6	35.312124948782355	37.0	35.0	37.0	33.0	37.0
7	35.31176388252536	37.0	35.0	37.0	33.0	37.0
8	35.2781975553078	37.0	35.0	37.0	33.0	37.0
9	36.90661993550693	39.0	37.0	39.0	33.0	39.0
10-11	36.900025330335666	39.0	37.0	39.0	33.0	39.0
12-13	36.87826509479745	39.0	37.0	39.0	33.0	39.0
14-15	38.23196036940638	40.0	38.0	41.0	33.0	41.0
16-17	38.261588991813895	40.0	38.0	41.0	33.0	41.0
18-19	38.29500711477306	40.0	38.5	41.0	33.5	41.0
20-21	38.367165879311365	40.0	39.0	41.0	34.0	41.0
22-23	38.346439562206555	40.0	39.0	41.0	33.5	41.0
24-25	38.346744688620916	40.0	38.5	41.0	33.0	41.0
26-27	38.31465422880994	40.0	38.0	41.0	33.0	41.0
28-29	38.2870905373034	40.0	38.0	41.0	33.0	41.0
30-31	38.29799190494779	40.0	38.0	41.0	33.0	41.0
32-33	38.26497153606449	40.0	38.0	41.0	33.0	41.0
34-35	38.13255054685434	40.0	38.0	41.0	33.0	41.0
36-37	38.013467698736584	40.0	38.0	41.0	32.5	41.0
38-39	37.95084776710427	40.0	38.0	41.0	32.0	41.0
40-41	37.94987426854418	40.0	38.0	41.0	32.0	41.0
42-43	37.80262558857916	40.0	38.0	41.0	32.0	41.0
44-45	37.62345838511603	40.0	37.0	41.0	31.5	41.0
46-47	37.45508926642766	40.0	37.0	41.0	31.0	41.0
48-49	37.28537299040353	40.0	37.0	41.0	31.0	41.0
50-51	37.124242995895806	40.0	36.0	41.0	30.0	41.0
52-53	37.08353780031946	40.0	36.0	41.0	30.0	41.0
54-55	36.90276396098644	39.0	36.0	41.0	30.0	41.0
56-57	36.62811592188376	39.0	35.0	41.0	29.5	41.0
58-59	36.37300093765832	39.0	35.0	41.0	29.0	41.0
60-61	36.10670040654462	38.5	35.0	40.0	28.5	41.0
62-63	35.942431363508234	38.0	35.0	40.0	28.5	41.0
64-65	35.73314007045998	37.5	35.0	40.0	28.5	41.0
66-67	35.378292580391125	37.0	34.0	40.0	28.0	41.0
68-69	34.9816399866713	36.5	34.0	39.0	28.0	41.0
70-71	34.574507632519314	36.0	34.0	39.0	27.5	41.0
72-73	34.22987945569322	35.0	33.0	38.5	26.5	40.0
74-75	33.97453526341127	35.0	33.0	37.0	26.5	40.0
76-77	33.69729268113127	35.0	33.0	37.0	26.0	39.0
78-79	33.352048560626685	35.0	33.0	36.5	26.0	39.0
80-81	32.95274826877085	35.0	32.5	36.0	26.0	38.5
82-83	32.69765047817668	35.0	32.0	36.0	25.5	37.0
84-85	32.51369048945183	35.0	32.0	35.0	25.0	37.0
86-87	32.448920990663126	35.0	32.0	35.0	25.5	36.5
88-89	32.311293963630874	35.0	32.0	35.0	25.0	36.0
90-91	32.044518791428175	35.0	32.0	35.0	25.0	36.0
92-93	31.875280667868445	35.0	32.0	35.0	24.5	36.0
94-95	31.78050743491363	35.0	32.0	35.0	24.5	35.0
96-97	31.704330203930997	35.0	32.0	35.0	24.0	35.0
98-99	31.739733706975965	35.0	32.0	35.0	24.0	35.0
100	30.731302531386852	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30634172419742
1101	2	0.3041342217432579
1101	3	0.3815045760069111
1101	4	0.3551117932463157
1101	5	0.3206374870250883
1101	6	0.26112725494780165
1101	7	0.24350535463723588
1101	8	0.37556974546163957
1101	9	0.4621640980234787
1101	10-11	0.4653527428808104
1101	12-13	0.42371812352671867
1101	14-15	0.491678017601032
1101	16-17	0.5017978711596314
1101	18-19	0.4574226588777748
1101	20-21	0.5157680068322605
1101	22-23	0.44737089239290384
1101	24-25	0.5136093328771452
1101	26-27	0.49231059293131096
1101	28-29	0.5397872551647538
1101	30-31	0.5298917234571476
1101	32-33	0.5401725577531664
1101	34-35	0.5141435739374671
1101	36-37	0.5173300714178737
1101	38-39	0.49100022404213917
1101	40-41	0.48628338561428563
1101	42-43	0.50995064449598
1101	44-45	0.4886608913480259
1101	46-47	0.45105632195195966
1101	48-49	0.4076967225601962
1101	50-51	0.45819911959003434
1101	52-53	0.4516282960884723
1101	54-55	0.5380117335694479
1101	56-57	0.5044380038400149
1101	58-59	0.6164119028190242
1101	60-61	0.5557051340021744
1101	62-63	0.5584090759785738
1101	64-65	0.5625068425485509
1101	66-67	0.6183726308063271
1101	68-69	0.466105640062743
1101	70-71	0.473251685200637
1101	72-73	0.4541739712027706
1101	74-75	0.4716729302017342
1101	76-77	0.5366835650401001
1101	78-79	0.5015786938312417
1101	80-81	0.45236718508221685
1101	82-83	0.3966743022826762
1101	84-85	0.476819584477262
1101	86-87	0.38239036364645784
1101	88-89	0.3395580827283098
1101	90-91	0.4051981819116435
1101	92-93	0.4521487525227279
1101	94-95	0.3578432732666741
1101	96-97	0.3748478831020776
1101	98-99	0.36689382031854834
1101	100	0.30371735958586044
1102	1	0.25645769307287125
1102	2	0.21944168331056346
1102	3	0.22761383528528256
1102	4	0.21224132063337464
1102	5	0.237474000580157
1102	6	0.1774847572783571
1102	7	0.18933215209030152
1102	8	0.1862366890432341
1102	9	0.29847426682118083
1102	10-11	0.2863466997715207
1102	12-13	0.30792597483888073
1102	14-15	0.32719657090112975
1102	16-17	0.3659547598054331
1102	18-19	0.3054870923373585
1102	20-21	0.3599141370751511
1102	22-23	0.3319116651059417
1102	24-25	0.328904604167775
1102	26-27	0.31536560956340765
1102	28-29	0.3844407761122426
1102	30-31	0.373433714767863
1102	32-33	0.35462246453086976
1102	34-35	0.3907247624700929
1102	36-37	0.42441995353122763
1102	38-39	0.435160049534133
1102	40-41	0.39900379957630605
1102	42-43	0.42920941117537126
1102	44-45	0.3841180890721603
1102	46-47	0.33481622897046037
1102	48-49	0.33978731416056007
1102	50-51	0.3571482833413526
1102	52-53	0.391380917320447
1102	54-55	0.41908988721768026
1102	56-57	0.39480602979777757
1102	58-59	0.3901188735073191
1102	60-61	0.34844753346141744
1102	62-63	0.36415056729445183
1102	64-65	0.30435945930860697
1102	66-67	0.3501807390817788
1102	68-69	0.3984717491156289
1102	70-71	0.3747933789568094
1102	72-73	0.36943682108735487
1102	74-75	0.2828411371001849
1102	76-77	0.2934440086887733
1102	78-79	0.31090341700231505
1102	80-81	0.30761001453358716
1102	82-83	0.2903470326982571
1102	84-85	0.29732259786205617
1102	86-87	0.23260529520891282
1102	88-89	0.25387430495512575
1102	90-91	0.33711333672600574
1102	92-93	0.3403854150270682
1102	94-95	0.35442408340623643
1102	96-97	0.3498710381778807
1102	98-99	0.31665418570865356
1102	100	0.23112759443035813
1103	1	0.18075307405218766
1103	2	0.20098902980103261
1103	3	0.2770792171068521
1103	4	0.17412094314920523
1103	5	0.22814335391213092
1103	6	0.2762551931859534
1103	7	0.19161500527413722
1103	8	0.2982281775644111
1103	9	0.3924566752344987
1103	10-11	0.37731206085737057
1103	12-13	0.3590851657438918
1103	14-15	0.4315385025040399
1103	16-17	0.39650424784690586
1103	18-19	0.3223295209562451
1103	20-21	0.4247477874298653
1103	22-23	0.3556444453727252
1103	24-25	0.39739233738584545
1103	26-27	0.38599200788847554
1103	28-29	0.3881384681196707
1103	30-31	0.4254443528337717
1103	32-33	0.42613494080770664
1103	34-35	0.4257005786344621
1103	36-37	0.4544663331385692
1103	38-39	0.4027117466780368
1103	40-41	0.4276583206688116
1103	42-43	0.3683430171941211
1103	44-45	0.30264733648521513
1103	46-47	0.35475405173691144
1103	48-49	0.31128243228790353
1103	50-51	0.2755239367837632
1103	52-53	0.34954457046700327
1103	54-55	0.3515399643673831
1103	56-57	0.363640669481498
1103	58-59	0.35099911980493204
1103	60-61	0.3474409959376672
1103	62-63	0.3767137417665083
1103	64-65	0.35372030744660776
1103	66-67	0.36596158432105597
1103	68-69	0.31848456250824597
1103	70-71	0.35447686207900375
1103	72-73	0.3902982384957028
1103	74-75	0.34238140693818053
1103	76-77	0.34368270462145034
1103	78-79	0.3453944020944064
1103	80-81	0.3158928660072533
1103	82-83	0.3299947990763741
1103	84-85	0.26450169334854934
1103	86-87	0.3431716839314163
1103	88-89	0.27093413798340293
1103	90-91	0.3191838860601379
1103	92-93	0.33307382897532634
1103	94-95	0.3328786009929061
1103	96-97	0.3810339401355449
1103	98-99	0.3095750748734609
1103	100	0.28070028894958554
1104	1	0.17112837163181638
1104	2	0.16609125668188085
1104	3	0.21448217734450736
1104	4	0.13965190812013617
1104	5	0.1769824030355167
1104	6	0.13431502513085292
1104	7	0.1570348657737597
1104	8	0.15709669662727066
1104	9	0.19363842034590562
1104	10-11	0.2640817996333311
1104	12-13	0.256915553160006
1104	14-15	0.3498819987672661
1104	16-17	0.2876282590103827
1104	18-19	0.2675454270249844
1104	20-21	0.22768058880764386
1104	22-23	0.2264050123433492
1104	24-25	0.19173685061501544
1104	26-27	0.2659559416677837
1104	28-29	0.3062946816792902
1104	30-31	0.23376649496566415
1104	32-33	0.23075665128089895
1104	34-35	0.22745796738463042
1104	36-37	0.2405405283141988
1104	38-39	0.2384047882406648
1104	40-41	0.2506377401637607
1104	42-43	0.19549736721344857
1104	44-45	0.1712613193093162
1104	46-47	0.11277197892452051
1104	48-49	0.1819996611122363
1104	50-51	0.22025935470885116
1104	52-53	0.23694782680378523
1104	54-55	0.25452961004977226
1104	56-57	0.1587331022104692
1104	58-59	0.24993728811413263
1104	60-61	0.26859151201154674
1104	62-63	0.2093496270437072
1104	64-65	0.2158292277830256
1104	66-67	0.23470430297411582
1104	68-69	0.31924328289699133
1104	70-71	0.2742423744435598
1104	72-73	0.26734401434541866
1104	74-75	0.1719673701702007
1104	76-77	0.13381328972410955
1104	78-79	0.14299651053559614
1104	80-81	0.20758323406386125
1104	82-83	0.1835670130795961
1104	84-85	0.20549542762164918
1104	86-87	0.23291930517863335
1104	88-89	0.16717100733261958
1104	90-91	0.28904139777220195
1104	92-93	0.2782756703351019
1104	94-95	0.21993918987486438
1104	96-97	0.17303738592194406
1104	98-99	0.18116273421516738
1104	100	0.18573941172090258
1105	1	0.12241446034740378
1105	2	0.16774424944095756
1105	3	0.14896628747684915
1105	4	0.19314462132092558
1105	5	0.10703596414428063
1105	6	0.12790208577195017
1105	7	0.12940062792966955
1105	8	0.15771357773213168
1105	9	0.1639485927051112
1105	10-11	0.16226306023494175
1105	12-13	0.21305685586301593
1105	14-15	0.24651383574107655
1105	16-17	0.2111183985205045
1105	18-19	0.1917256320891596
1105	20-21	0.26785560877986114
1105	22-23	0.22857639119781936
1105	24-25	0.20783240035197537
1105	26-27	0.16561519787478574
1105	28-29	0.21271990401589846
1105	30-31	0.20099356786152356
1105	32-33	0.19022710331849169
1105	34-35	0.18577105427137752
1105	36-37	0.1703475441493083
1105	38-39	0.14430309755528725
1105	40-41	0.19427001153043477
1105	42-43	0.18184506717708615
1105	44-45	0.15486567580018118
1105	46-47	0.15547499243646
1105	48-49	0.1764024093526828
1105	50-51	0.19929640108235702
1105	52-53	0.16699523291272556
1105	54-55	0.22351504629934738
1105	56-57	0.1809415575829405
1105	58-59	0.2176759766055696
1105	60-61	0.17178608002303264
1105	62-63	0.15999313377709967
1105	64-65	0.13531444401386494
1105	66-67	0.19047535991909115
1105	68-69	0.24486261699446743
1105	70-71	0.13717444642427523
1105	72-73	0.19614231578164976
1105	74-75	0.2060815167737715
1105	76-77	0.16396918596174004
1105	78-79	0.147600630554372
1105	80-81	0.184095004104897
1105	82-83	0.25927721899407175
1105	84-85	0.2273049489320016
1105	86-87	0.18365834951912774
1105	88-89	0.1530877522228309
1105	90-91	0.2011463788904635
1105	92-93	0.18032666940265685
1105	94-95	0.1314731971796803
1105	96-97	0.13049446245435803
1105	98-99	0.07197564936239687
1105	100	0.1411091176404149
1106	1	0.10336077061300486
1106	2	0.13355395843979778
1106	3	0.20904040190305295
1106	4	0.2192292871641115
1106	5	0.19185621707458722
1106	6	0.14057713624708157
1106	7	0.18522826331735587
1106	8	0.1590787827526441
1106	9	0.21833422624460752
1106	10-11	0.23397518655252725
1106	12-13	0.22473976674250906
1106	14-15	0.2658589116067205
1106	16-17	0.28242788320732615
1106	18-19	0.25774397293886864
1106	20-21	0.2555676238841613
1106	22-23	0.28967278424584464
1106	24-25	0.30969926960393224
1106	26-27	0.2618424812521596
1106	28-29	0.29223288830797856
1106	30-31	0.24560674176212416
1106	32-33	0.20298173658170526
1106	34-35	0.2159230253158455
1106	36-37	0.25466020353648844
1106	38-39	0.2856416085725826
1106	40-41	0.24314718225087972
1106	42-43	0.19189758618901465
1106	44-45	0.2191795294920098
1106	46-47	0.18338522215099573
1106	48-49	0.216389031677366
1106	50-51	0.17904750619405974
1106	52-53	0.1444944096473506
1106	54-55	0.1582890157036445
1106	56-57	0.14478425190269206
1106	58-59	0.15276526607366492
1106	60-61	0.16651163787783219
1106	62-63	0.19405353992735286
1106	64-65	0.13789134482258447
1106	66-67	0.11249576541872841
1106	68-69	0.18080308771821763
1106	70-71	0.1546791843861257
1106	72-73	0.16085280862592555
1106	74-75	0.12230018787516883
1106	76-77	0.05956773998637743
1106	78-79	0.15861849891644653
1106	80-81	0.15134646168279886
1106	82-83	0.2123296213333532
1106	84-85	0.23055841600344706
1106	86-87	0.17733592193566494
1106	88-89	0.12238382332639475
1106	90-91	0.16239955115663918
1106	92-93	0.09228391858701102
1106	94-95	0.06876760244776747
1106	96-97	0.1124378792045988
1106	98-99	0.07494048812198528
1106	100	-0.01755692737248893
1107	1	0.09014852768800807
1107	2	0.05767086049989345
1107	3	0.10817956698221565
1107	4	0.11894838325202528
1107	5	0.07034007373621876
1107	6	0.013692013098520306
1107	7	0.0673252596256404
1107	8	0.07531596826894571
1107	9	0.06963635744066465
1107	10-11	0.16446624856950365
1107	12-13	0.1260920200412059
1107	14-15	0.12746252846255146
1107	16-17	0.10384031408816696
1107	18-19	0.1592159122716481
1107	20-21	0.11929139723580562
1107	22-23	0.10937570460141899
1107	24-25	0.14400631089705485
1107	26-27	0.17243607385091764
1107	28-29	0.16333668288108072
1107	30-31	0.1631912738038963
1107	32-33	0.2037382405899706
1107	34-35	0.20922665573409205
1107	36-37	0.226536314950593
1107	38-39	0.19187288634481803
1107	40-41	0.18478284488567454
1107	42-43	0.1805072654843869
1107	44-45	0.1968830947844893
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2216	78-79	-0.8425287928603424
2216	80-81	-0.86905554116084
2216	82-83	-0.8110093255347444
2216	84-85	-0.7210841985106207
2216	86-87	-0.6905784040509033
2216	88-89	-0.779057134170543
2216	90-91	-0.8484377055048249
2216	92-93	-0.8737780228765253
2216	94-95	-0.8718861694788202
2216	96-97	-0.837031293475107
2216	98-99	-0.7784190690233892
2216	100	-0.8879212744610321
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	103.0
1	56.0
2	55.0
3	44.0
4	50.0
5	61.0
6	120.0
7	638.0
8	1812.0
9	2765.0
10	3201.0
11	4303.0
12	6131.0
13	7639.0
14	9041.0
15	9694.0
16	10497.0
17	11321.0
18	12558.0
19	13629.0
20	15155.0
21	17136.0
22	20005.0
23	22798.0
24	27389.0
25	33654.0
26	37888.0
27	39317.0
28	43856.0
29	55010.0
30	65188.0
31	77306.0
32	100020.0
33	136077.0
34	201640.0
35	308938.0
36	517447.0
37	930102.0
38	1070382.0
39	315765.0
40	645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38783548940187	18.698939363540585	13.128586784060698	22.78463836299684
2	14.043463087423866	22.668268642770872	37.795826514803466	25.492441755001792
3	22.259239767370723	25.024915396637372	30.17668073876966	22.53916409722224
4	11.694745642358033	16.68044701613052	41.3238532278955	30.30095411361595
5	12.157032943825188	39.92699870766739	35.28922965738654	12.626738691120881
6	28.863138493880413	37.94352324014187	17.935627779910412	15.257710486067296
7	25.575543261886473	33.071942181223235	23.107725347248795	18.2447892096415
8	24.429843340721337	31.70303999597018	21.15992478959686	22.70719187371162
9	23.971665866664214	18.704000867974408	22.84153121922796	34.48280204613342
10-11	24.970780939630732	24.873860359773456	28.408192089711353	21.74716661088446
12-13	27.71355489058887	23.41598703476415	27.066915690124667	21.803542384522316
14-15	23.361897398234085	25.312395367184365	27.888937393777823	23.436769840803727
16-17	21.505345484809986	28.192544741877995	27.988587192851227	22.313522580460788
18-19	20.92418447846594	30.75138379919312	26.96652302432854	21.357908698012398
20-21	23.20043950817127	27.20591422809622	25.47301982170228	24.120626442030233
22-23	21.98261535426964	27.425612389989812	26.129579991541007	24.462192264199537
24-25	24.418985650549356	27.327417542059923	25.38745793159933	22.86613887579139
26-27	23.490284771600773	25.4703953924682	28.78789529983612	22.25142453609491
28-29	21.54955758288275	30.004711576103944	26.49677303656176	21.94895780445155
30-31	22.65481155410901	26.86219303517385	26.259636991467705	24.22335841924944
32-33	22.949674838727177	27.559101076572983	26.85564253863137	22.63558154606847
34-35	24.508914461614474	26.78569568887118	26.631528724510865	22.073861125003482
36-37	23.402637684480116	25.7446114355207	28.74222787479924	22.110523005199944
38-39	22.803986546809323	27.75362427658582	27.637098098523357	21.805291078081503
40-41	22.546614051457865	28.217370163979496	26.97231040874073	22.26370537582191
42-43	22.039631964505897	28.063632920794568	25.075000717579833	24.8217343971197
44-45	23.080577020481257	26.316936878059817	26.041158236382874	24.56132786507606
46-47	24.53863090702685	27.808882718366313	26.50940312305796	21.143083251548877
48-49	23.67203899362949	27.091345626860768	26.866669339365718	22.369946040144022
50-51	20.503008816244012	27.690581072224553	29.30246551788666	22.503944593644782
52-53	22.524962992443943	28.95991750421672	25.402516974059708	23.112602529279634
54-55	21.67650911680791	26.670631505570864	26.869301427281446	24.783557950339787
56-57	23.849800948634257	25.446548294515107	26.68921120521223	24.014439551638404
58-59	25.6788260817376	26.01941858114439	26.007330266680572	22.294425070437445
60-61	23.315002036137873	26.939763507612685	28.771015765109393	20.974218691140052
62-63	21.7972730895336	27.383636357029783	27.91535604022659	22.903734513210026
64-65	20.991339172463647	29.91740334088176	26.267510850259157	22.823746636395438
66-67	23.042667501633694	26.626105357254247	25.261539896424516	25.069687244687533
68-69	22.06782722937044	26.909126939760718	26.448360523586857	24.574685307281985
70-71	24.23954393349127	26.630334324252104	25.7185332011893	23.41158854106733
72-73	23.39117232207259	25.05573848726345	29.016317293011777	22.536771897652184
74-75	21.801975789444867	29.614057519751718	26.29508106967542	22.288885621127996
76-77	22.38789428050855	26.447097253860164	26.21082510652596	24.95418335910532
78-79	22.97843775141361	26.918216947127465	26.901937518486964	23.201407782971962
80-81	24.25143252245085	26.10140456488433	26.677563011121975	22.96959990154285
82-83	23.151916239478364	25.36103725329096	28.950036744189823	22.537009763040857
84-85	22.502078472259903	27.518960608832487	27.41914306865838	22.55981785024922
86-87	22.152814727975823	28.167930822688746	26.77907709242217	22.900177356913257
88-89	21.898511733285627	27.755434795115484	25.16945979855828	25.176593673040603
90-91	23.0218073742902	25.75891469657099	25.958219509754976	25.261058419383836
92-93	24.483642361220877	27.273196350167893	26.482963828590687	21.760197460020535
94-95	23.154049760239776	26.672458994630166	27.152294377202406	23.021196867927653
96-97	20.733084855995866	27.11479875854999	29.38766146747298	22.764454917981162
98-99	22.5943498834116	28.279057835888686	25.823894590327306	23.30269769037241
100	22.41774021369123	24.570349052259612	25.75960572565949	27.252305008389666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1737.0
1	1195.0
2	686.0
3	698.0
4	614.5
5	472.5
6	386.5
7	394.5
8	448.0
9	484.0
10	410.0
11	343.0
12	362.0
13	391.5
14	430.0
15	523.0
16	634.5
17	745.5
18	973.0
19	1382.0
20	1880.0
21	2728.0
22	4138.0
23	5855.0
24	8435.5
25	12149.0
26	16388.5
27	20670.5
28	25413.5
29	31683.5
30	38915.0
31	46535.5
32	54765.0
33	62365.0
34	69361.0
35	77021.0
36	84910.5
37	93035.0
38	98474.0
39	100971.5
40	103681.0
41	106439.0
42	108842.0
43	114289.0
44	126970.0
45	140602.5
46	149685.0
47	216092.5
48	367153.5
49	370804.5
50	244280.5
51	178490.5
52	142794.5
53	126449.5
54	116550.5
55	104093.5
56	92089.5
57	80875.0
58	70647.5
59	63342.0
60	53955.0
61	42810.5
62	33663.5
63	26500.5
64	19579.5
65	13894.0
66	10397.0
67	7876.5
68	6092.5
69	4716.0
70	3640.0
71	2832.5
72	2284.5
73	2020.0
74	1716.5
75	1373.0
76	1107.5
77	808.0
78	547.0
79	366.0
80	256.0
81	187.0
82	129.5
83	89.0
84	62.0
85	43.5
86	29.0
87	21.5
88	21.0
89	15.5
90	10.5
91	8.0
92	8.5
93	10.5
94	9.5
95	9.5
96	9.0
97	5.0
98	5.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06005662758788367
2	0.008935844991906885
3	0.011212184908544412
4	0.008911628609814997
5	0.011333266819003854
6	0.011648079786198407
7	0.0029059658510266293
8	0.005327604060215487
9	0.006829019749912579
10-11	0.006829019749912579
12-13	0.005811931702053259
14-15	0.010013473994995928
16-17	0.00945649720688249
18-19	0.007616052167898958
20-21	0.006659505075269359
22-23	0.002482179164418579
24-25	0.003608240931691398
26-27	0.00477062727210205
28-29	0.005920905421466757
30-31	0.008705789362033943
32-33	0.00988028389349054
34-35	0.01140591596527952
36-37	0.01789590636590566
38-39	0.018162286568916435
40-41	0.017399470533021943
42-43	0.02362308073063731
44-45	0.02724342985337465
46-47	0.02921706499386357
48-49	0.03086377897611199
50-51	0.0319050834060632
52-53	0.02966506806256351
54-55	0.035925002833316705
56-57	0.038915726021664945
58-59	0.03590078645122481
60-61	0.036881549925946305
62-63	0.03198984074338481
64-65	0.034484128098849334
66-67	0.03701474002745169
68-69	0.03970275843965132
70-71	0.03915788984258383
72-73	0.03519851137056004
74-75	0.0379470707379894
76-77	0.03799550350217318
78-79	0.03697841545431386
80-81	0.04242710142498879
82-83	0.03808026083949479
84-85	0.033999800457011564
86-87	0.03195351617024698
88-89	0.030137287513355336
90-91	0.03237730285685503
92-93	0.03415720694060884
94-95	0.03782598882752996
96-97	0.032934279644968464
98-99	0.02997988102975806
100	0.027679324731028646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4129436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.74668409880513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22996859132519	42.865345469074825
2	13.912641406967477	16.068178166162134
3	5.318731076114194	9.214172497765926
4	2.515298164789563	5.810005141456287
5	1.3687891676872257	3.9521517832150312
6	0.8287819833527592	2.8715646827671777
7	0.5155361974315995	2.083935415420727
8	0.3312482269953909	1.5302789378073707
9	0.23024338923710996	1.1966213037702256
>10	0.7390822322638008	6.23238664426254
>50	0.007344220299039318	0.2811973100446491
>100	0.001662438161026081	0.1396140352192376
>500	0.0	0.0
>1k	1.2234643193871503E-4	0.14532912254641395
>5k	1.8351964790807256E-4	0.8912271348379865
>10k+	3.670392958161451E-4	6.717992355649422
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	97398	2.3586271829857637	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	23852	0.5776091456557264	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	23745	0.5750179927718942	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	16719	0.4048736921942851	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	15578	0.3772428002274402	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	12882	0.31195543410770865	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9109	0.22058702447501308	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	8324	0.20157716453288052	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	7796	0.18879091478836335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.421638209188858E-5
2	0.0	0.0	0.0	0.0	2.421638209188858E-5
3	0.0	0.0	0.0	0.0	2.421638209188858E-5
4	0.0	0.0	0.0	0.0	2.421638209188858E-5
5	0.0	0.0	0.0	0.0	4.843276418377716E-5
6	0.0	0.0	0.0	0.0	4.843276418377716E-5
7	0.0	0.0	0.0	0.0	4.843276418377716E-5
8	0.0	0.0	0.0	0.0	4.843276418377716E-5
9	0.0	0.0	0.0	0.0	7.264914627566574E-5
10-11	0.0	0.0	0.0	2.421638209188858E-5	9.686552836755431E-5
12-13	0.0	0.0	0.0	3.632457313783287E-5	5.569767881134373E-4
14-15	0.0	1.210819104594429E-5	0.0	6.0540955229721446E-5	7.628160358944902E-4
16-17	0.0	2.421638209188858E-5	0.0	1.4529829255133147E-4	9.928716657674316E-4
18-19	2.421638209188858E-5	2.421638209188858E-5	0.0	2.7848839405671865E-4	0.0010655208120430974
20-21	2.421638209188858E-5	2.421638209188858E-5	0.0	4.1167849556210585E-4	0.0012350354866863175
22-23	2.421638209188858E-5	2.421638209188858E-5	0.0	6.538423164809916E-4	0.0013561173971457603
24-25	2.421638209188858E-5	2.421638209188858E-5	0.0	0.0011381699583187632	0.0013803337792376489
26-27	2.421638209188858E-5	2.421638209188858E-5	0.0	0.0019009859942132534	0.0014893074986511475
28-29	2.421638209188858E-5	2.421638209188858E-5	0.0	0.004661653552688551	0.0015377402628349247
30-31	2.421638209188858E-5	2.421638209188858E-5	0.0	0.011853919033979459	0.001549848453880869
32-33	2.421638209188858E-5	2.421638209188858E-5	0.0	0.03046420867159583	0.0016224976001565348
34-35	2.421638209188858E-5	2.421638209188858E-5	0.0	0.05677530781443277	0.0016709303643403118
36-37	2.421638209188858E-5	2.421638209188858E-5	0.0	0.09452864749568707	0.0016709303643403118
38-39	2.421638209188858E-5	2.421638209188858E-5	0.0	0.14677549185893668	0.0017435795106159775
40-41	2.421638209188858E-5	2.421638209188858E-5	0.0	0.2049916744078368	0.0017435795106159775
42-43	2.421638209188858E-5	2.421638209188858E-5	0.0	0.2602413501504806	0.0017677958927078661
44-45	2.421638209188858E-5	2.421638209188858E-5	0.0	0.3272480793987363	0.0018646614210754205
46-47	2.421638209188858E-5	2.421638209188858E-5	0.0	0.40914788363350346	0.002022067904672696
48-49	2.421638209188858E-5	2.421638209188858E-5	0.0	0.48539316264981464	0.002179474388269972
50-51	2.421638209188858E-5	2.421638209188858E-5	0.0	0.566602799994963	0.0023247726808213035
52-53	2.421638209188858E-5	2.421638209188858E-5	0.0	0.6469164312027115	0.0024458545912807465
54-55	2.421638209188858E-5	2.421638209188858E-5	0.0	0.735257793073921	0.0024942873554645237
56-57	2.421638209188858E-5	2.421638209188858E-5	0.0	0.8473917503504111	0.0025306119286023564
58-59	2.421638209188858E-5	2.421638209188858E-5	0.0	0.9575762888685041	0.0025911528838320776
60-61	2.421638209188858E-5	2.421638209188858E-5	0.0	1.0699887345390509	0.0026638020301077434
62-63	2.421638209188858E-5	2.421638209188858E-5	0.0	1.183599891123146	0.002857533086842852
64-65	2.421638209188858E-5	2.421638209188858E-5	0.0	1.3032651432302136	0.0028817494689347407
66-67	2.421638209188858E-5	2.421638209188858E-5	0.0	1.439409643350811	0.0029422904241644624
68-69	2.421638209188858E-5	2.421638209188858E-5	0.0	1.5881708785412827	0.0030149395704401278
70-71	2.421638209188858E-5	2.421638209188858E-5	0.0	1.7450809263056746	0.003051264143577961
72-73	2.421638209188858E-5	2.421638209188858E-5	0.0	1.8890231014598604	0.0032692115824049578
74-75	2.421638209188858E-5	2.421638209188858E-5	0.0	2.0376511465488267	0.0034024016839103452
76-77	2.421638209188858E-5	2.421638209188858E-5	0.0	2.192030582384616	0.0034508344480941224
78-79	2.421638209188858E-5	2.421638209188858E-5	0.0	2.393341366714486	0.003487159021231955
80-81	2.421638209188858E-5	2.421638209188858E-5	0.0	2.6013116561196252	0.003559808167507621
82-83	2.421638209188858E-5	2.421638209188858E-5	0.0	2.794957955517412	0.003729322842150841
84-85	2.421638209188858E-5	2.421638209188858E-5	0.0	2.9850202303655995	0.003777755606334618
86-87	2.421638209188858E-5	2.421638209188858E-5	0.0	3.180906545106886	0.003862512943656228
88	2.421638209188858E-5	2.421638209188858E-5	0.0	3.3449119928242017	0.003947270280977838
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1310	0.0	21.891708	1
CATGGGG	6805	0.0	19.474598	4
CTAACGC	630	0.0	19.394579	3
GTATAAG	1695	0.0	19.138176	1
TAACGCC	695	0.0	18.933058	4
CGCCTAC	605	0.0	18.640888	7
TACCTGG	3495	0.0	18.555792	2
TAGTACT	1960	0.0	18.462147	4
GTACCTG	3735	0.0	17.873882	1
GAGTACG	1730	0.0	17.663973	1
CTAGTAC	1760	0.0	17.62296	3
ATGGGGG	4400	0.0	17.302542	5
GTACGCA	1710	0.0	16.76414	3
GCTATTG	1910	0.0	16.72983	8
GTCTAAC	1245	0.0	16.61516	1
TACGGGC	910	0.0	16.525558	4
GGCGTCG	825	0.0	16.518099	8
GTATAAT	1710	0.0	16.49591	1
GGGCTAT	2225	0.0	16.473896	6
TCTAACG	690	0.0	16.345932	2
>>END_MODULE