FastQCFastQC Report
Mon 16 Jan 2017
ERR1146553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4129436
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1082902.622392016730614No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC282150.6832652207226362No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT262560.6358253282046266No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC216010.5230980695668852No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG178390.4319960401372004No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC153370.37140665214329516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102440.24807261814930656No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93660.2268106346726284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93280.22589041215313665No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG75430.18266417011911554No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA52470.12706335683613937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49880.12079131387434024No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG47500.11502781493647074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT113850.021.8160512-13
GAGTACG17850.021.0934371
GTACGCA17400.020.5355933
CTAACGC7400.020.3311163
TACGCAG18000.020.1122744
TAACGCC7500.020.0600344
CTAGTAC18050.020.056563
AGTACTT120400.019.7314412-13
TAGTACT19850.019.185254
GTACTTT129050.019.10080114-15
CATGGGG67850.018.8478584
TCTAACG7250.018.824982
TACCTGG37200.017.7116762
AGTACGC20600.017.5913052
CGCAGTG20650.017.5246986
GTATAAG15500.017.3076761
CGATAAT7500.016.92022366-67
GTACCTG40450.016.7548121
ACTTTTT152500.016.50285116-17
CGCCTAC8600.016.3940757
Produced by FastQC (version 0.11.5)