##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4129436
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51933678110037	34.0	31.0	34.0	31.0	34.0
2	32.52277962414238	34.0	31.0	34.0	31.0	34.0
3	32.68372678496531	34.0	31.0	34.0	31.0	34.0
4	36.11105802342015	37.0	35.0	37.0	35.0	37.0
5	36.144614179757234	37.0	35.0	37.0	35.0	37.0
6	36.26234623808191	37.0	37.0	37.0	35.0	37.0
7	36.260198244990356	37.0	37.0	37.0	35.0	37.0
8	36.26846668649181	37.0	37.0	37.0	35.0	37.0
9	38.06714936373878	39.0	38.0	39.0	35.0	39.0
10-11	38.044251079324155	39.0	38.0	39.0	35.0	39.0
12-13	38.03758915261067	39.0	38.0	39.0	35.0	39.0
14-15	39.424265928809646	41.0	39.0	41.0	36.5	41.0
16-17	39.46065406995048	41.0	39.0	41.0	36.5	41.0
18-19	39.528004913988255	41.0	39.0	41.0	37.0	41.0
20-21	39.557176936511425	41.0	39.0	41.0	37.0	41.0
22-23	39.51203626839113	41.0	39.0	41.0	37.0	41.0
24-25	39.48601419176856	41.0	39.0	41.0	36.5	41.0
26-27	39.37665579512553	41.0	39.0	41.0	36.0	41.0
28-29	39.319832054546914	41.0	39.0	41.0	36.0	41.0
30-31	39.28850150480598	41.0	39.0	41.0	36.0	41.0
32-33	39.172341574006715	41.0	39.0	41.0	35.0	41.0
34-35	39.07266161771244	40.0	39.0	41.0	35.0	41.0
36-37	38.97923287829137	40.0	38.5	41.0	35.0	41.0
38-39	38.89470499119008	40.0	38.0	41.0	35.0	41.0
40-41	38.88989937124586	40.0	39.0	41.0	35.0	41.0
42-43	38.81680331163868	40.0	38.0	41.0	35.0	41.0
44-45	38.697008380805514	40.0	38.0	41.0	35.0	41.0
46-47	38.5029345411819	40.0	38.0	41.0	35.0	41.0
48-49	38.30024753985774	40.0	38.0	41.0	34.0	41.0
50-51	38.12773850956886	40.0	38.0	41.0	34.0	41.0
52-53	38.09583778511157	40.0	38.0	41.0	34.0	41.0
54-55	37.98536579813805	40.0	37.0	41.0	34.0	41.0
56-57	37.75086779405226	40.0	37.0	41.0	33.0	41.0
58-59	37.55019850168401	40.0	36.5	41.0	33.0	41.0
60-61	37.26541493802059	39.0	36.0	41.0	32.5	41.0
62-63	37.08065120757411	39.0	35.0	41.0	32.0	41.0
64-65	36.80335026381327	39.0	35.0	40.5	32.0	41.0
66-67	36.48826086661714	37.5	35.0	40.0	32.0	41.0
68-69	36.08699020883239	37.0	35.0	39.5	31.5	41.0
70-71	35.67494362426249	36.5	35.0	39.0	31.0	41.0
72-73	35.30474936528863	36.0	35.0	39.0	31.0	41.0
74-75	35.00943724518312	35.5	35.0	37.5	31.0	40.0
76-77	34.70685960504049	35.0	34.5	37.0	31.0	39.0
78-79	34.39177795708663	35.0	34.0	37.0	30.5	39.0
80-81	34.08871005628856	35.0	34.0	36.0	30.0	39.0
82-83	33.802607426292596	35.0	34.0	36.0	29.5	37.0
84-85	33.613095953055094	35.0	34.0	35.5	30.0	37.0
86-87	33.52450867382374	35.0	34.0	35.0	30.0	37.0
88-89	33.37038786894869	35.0	34.0	35.0	30.0	36.0
90-91	33.248337666451306	35.0	34.0	35.0	29.0	36.0
92-93	33.14376079445232	35.0	34.0	35.0	29.0	36.0
94-95	33.02747118492695	35.0	34.0	35.0	29.0	36.0
96-97	32.97398361422722	35.0	34.0	35.0	29.0	35.0
98-99	32.98991714607031	35.0	34.0	35.0	29.0	35.0
100	32.36136024386865	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2484260954602462
1101	2	0.22976990506610662
1101	3	0.2666993310653183
1101	4	0.17870734539184951
1101	5	0.16970246928551092
1101	6	0.14393740931064514
1101	7	0.1712888773418868
1101	8	0.14404652391005612
1101	9	0.16722775538432444
1101	10-11	0.19111007319282436
1101	12-13	0.21320396548905762
1101	14-15	0.3426567463401824
1101	16-17	0.34665124690339155
1101	18-19	0.3119709224892375
1101	20-21	0.2902015131175091
1101	22-23	0.29935231312666843
1101	24-25	0.27398321793495484
1101	26-27	0.32431226837683624
1101	28-29	0.3593230565909309
1101	30-31	0.33321512792792873
1101	32-33	0.287186237838732
1101	34-35	0.2745568647050689
1101	36-37	0.3553181273808903
1101	38-39	0.3772102723888793
1101	40-41	0.3860691881252194
1101	42-43	0.34105864439872136
1101	44-45	0.35839592926920716
1101	46-47	0.36439077133955067
1101	48-49	0.3512220281573448
1101	50-51	0.36788356146664825
1101	52-53	0.38718688273380764
1101	54-55	0.2931215561901084
1101	56-57	0.33850896780710116
1101	58-59	0.38197690801416684
1101	60-61	0.3925530409319933
1101	62-63	0.3731485024342973
1101	64-65	0.35678504083212204
1101	66-67	0.36271951355733734
1101	68-69	0.375906441672079
1101	70-71	0.35005132522083926
1101	72-73	0.31505976869842556
1101	74-75	0.2752505348447727
1101	76-77	0.26863495047512487
1101	78-79	0.23971684360490997
1101	80-81	0.3199809852053974
1101	82-83	0.28671234107354593
1101	84-85	0.24238280832697967
1101	86-87	0.18705490838031835
1101	88-89	0.21093890547282967
1101	90-91	0.21740938117084596
1101	92-93	0.2112960895536915
1101	94-95	0.22116796751694778
1101	96-97	0.22408220494641995
1101	98-99	0.192880715186881
1101	100	0.20519644098802559
1102	1	0.25810005823325355
1102	2	0.22668234663017728
1102	3	0.2307079154280558
1102	4	0.18409342671883877
1102	5	0.13293771281584554
1102	6	0.13501765272058464
1102	7	0.14975659181335743
1102	8	0.16533715386571401
1102	9	0.2512269315194189
1102	10-11	0.2147728621905287
1102	12-13	0.22639762878115022
1102	14-15	0.3117498800539664
1102	16-17	0.2857396378040704
1102	18-19	0.26402325339053334
1102	20-21	0.2676496087683944
1102	22-23	0.28417273121660713
1102	24-25	0.3164321477705556
1102	26-27	0.29314955677771337
1102	28-29	0.31026461034166175
1102	30-31	0.30784957012495084
1102	32-33	0.255294797089924
1102	34-35	0.2706693059251464
1102	36-37	0.30103667431711045
1102	38-39	0.35444767189972026
1102	40-41	0.33632511943655885
1102	42-43	0.2938934534650173
1102	44-45	0.3288385932596043
1102	46-47	0.2865500498815692
1102	48-49	0.22530775740678877
1102	50-51	0.2565191491626848
1102	52-53	0.2779658736626942
1102	54-55	0.29297525137041447
1102	56-57	0.2649652651277421
1102	58-59	0.2561917423983999
1102	60-61	0.28910521359618
1102	62-63	0.31021324290825447
1102	64-65	0.2986616081534095
1102	66-67	0.2330216346224816
1102	68-69	0.2192041974571879
1102	70-71	0.2129808013528418
1102	72-73	0.23627725699403612
1102	74-75	0.19722104684499442
1102	76-77	0.22350974966879988
1102	78-79	0.2039941730442365
1102	80-81	0.23724878085937462
1102	82-83	0.19770311172262467
1102	84-85	0.21691695486141782
1102	86-87	0.20687227782610762
1102	88-89	0.2508722738935205
1102	90-91	0.21954912947065708
1102	92-93	0.2102314290582683
1102	94-95	0.1730262644729521
1102	96-97	0.21944559988902768
1102	98-99	0.21250426568344238
1102	100	0.20128845758227953
1103	1	0.24355213328031056
1103	2	0.21626189890107383
1103	3	0.21441669356396886
1103	4	0.16536693343699937
1103	5	0.167662135090886
1103	6	0.13186539566947886
1103	7	0.1293872736679731
1103	8	0.176776430911616
1103	9	0.1562752885021581
1103	10-11	0.20373205301003594
1103	12-13	0.24814790315534907
1103	14-15	0.3119114904113971
1103	16-17	0.2780516022292687
1103	18-19	0.26871668752085043
1103	20-21	0.31640538003244956
1103	22-23	0.2941950317357751
1103	24-25	0.26048033330951625
1103	26-27	0.27923759850910557
1103	28-29	0.2931059085431329
1103	30-31	0.2928871445330472
1103	32-33	0.26534210873583675
1103	34-35	0.32093621953927
1103	36-37	0.29657635671427585
1103	38-39	0.3072181137928851
1103	40-41	0.26844812800575113
1103	42-43	0.2993512233532414
1103	44-45	0.2909501565661827
1103	46-47	0.29185614973197005
1103	48-49	0.29372729789466945
1103	50-51	0.3308076467378882
1103	52-53	0.3385034889009262
1103	54-55	0.3083818916749266
1103	56-57	0.3512204111136228
1103	58-59	0.32037328641007434
1103	60-61	0.3143178188620297
1103	62-63	0.31842223673840664
1103	64-65	0.21929927217402678
1103	66-67	0.258905906215098
1103	68-69	0.26394108681694206
1103	70-71	0.22573412451925634
1103	72-73	0.2333459315625106
1103	74-75	0.22910339279462022
1103	76-77	0.1846533609933303
1103	78-79	0.18418300918886388
1103	80-81	0.23635735864975516
1103	82-83	0.23882877916699385
1103	84-85	0.272899338477373
1103	86-87	0.1918062805170493
1103	88-89	0.2537488741246534
1103	90-91	0.2438091742170556
1103	92-93	0.23840058465270175
1103	94-95	0.3025400780968681
1103	96-97	0.2771635846848284
1103	98-99	0.18940809341192733
1103	100	0.1818411308014305
1104	1	0.12291226035529235
1104	2	0.13982114779985721
1104	3	0.12812358018383918
1104	4	0.1003548014478497
1104	5	0.04735665230393238
1104	6	0.026258341295985588
1104	7	0.07603229548779211
1104	8	0.07744775494851552
1104	9	0.08740680174102522
1104	10-11	0.12212053615338192
1104	12-13	0.125267232722301
1104	14-15	0.15174480544041558
1104	16-17	0.18105827361199545
1104	18-19	0.17617551224415706
1104	20-21	0.1743850296771825
1104	22-23	0.20806780116713952
1104	24-25	0.2029686616199271
1104	26-27	0.17015686384945639
1104	28-29	0.2010734854185472
1104	30-31	0.18062640609410607
1104	32-33	0.1784242934737037
1104	34-35	0.15329881877286056
1104	36-37	0.2056234504743415
1104	38-39	0.22370298231948738
1104	40-41	0.24173160766589774
1104	42-43	0.17316315921709702
1104	44-45	0.2012119297370134
1104	46-47	0.28163164467513724
1104	48-49	0.27901059881860846
1104	50-51	0.2941885618564939
1104	52-53	0.25902021823294064
1104	54-55	0.2542449762419139
1104	56-57	0.30715419742479355
1104	58-59	0.2438936050019862
1104	60-61	0.2585806894839351
1104	62-63	0.29043864762926574
1104	64-65	0.23451610960913882
1104	66-67	0.23767728053501713
1104	68-69	0.23394311509602517
1104	70-71	0.19734619185112479
1104	72-73	0.1932294111688151
1104	74-75	0.2263262857262518
1104	76-77	0.16288644054278478
1104	78-79	0.16545386398938433
1104	80-81	0.1816756881014996
1104	82-83	0.14612727293761196
1104	84-85	0.20371685204140988
1104	86-87	0.18589647823590383
1104	88-89	0.14607629274314604
1104	90-91	0.1316736386060242
1104	92-93	0.1737165506949765
1104	94-95	0.17365202725826379
1104	96-97	0.10276324600125264
1104	98-99	0.11019928645456645
1104	100	0.18781809375028047
1105	1	0.12511415900160472
1105	2	0.13606338529422146
1105	3	0.13132556121707495
1105	4	0.09767083120514286
1105	5	0.09681456795797772
1105	6	0.0544695878557917
1105	7	0.0821003146611261
1105	8	0.09992506371597187
1105	9	0.10004556846400448
1105	10-11	0.09858964153706751
1105	12-13	0.11025260604075271
1105	14-15	0.1711869486822195
1105	16-17	0.16884369146261946
1105	18-19	0.12515832496258383
1105	20-21	0.16396985422185395
1105	22-23	0.19222714348409653
1105	24-25	0.15857312824024916
1105	26-27	0.15505028747534055
1105	28-29	0.2225499461771534
1105	30-31	0.2271053018306901
1105	32-33	0.19914637643613275
1105	34-35	0.19709956899828995
1105	36-37	0.17370902574884184
1105	38-39	0.19049860473948854
1105	40-41	0.18176676202248387
1105	42-43	0.21037717390551336
1105	44-45	0.2110002409780094
1105	46-47	0.22739837990950207
1105	48-49	0.2517376294401217
1105	50-51	0.1798803219870848
1105	52-53	0.19856333289583716
1105	54-55	0.22106188595401477
1105	56-57	0.22903388873564268
1105	58-59	0.1955672034995075
1105	60-61	0.23488509607950192
1105	62-63	0.27933079449800147
1105	64-65	0.18478048336523045
1105	66-67	0.16655938004208082
1105	68-69	0.18631835354972281
1105	70-71	0.15457352175575068
1105	72-73	0.17926749962883548
1105	74-75	0.1757538197006454
1105	76-77	0.12499752261697239
1105	78-79	0.16432167428904165
1105	80-81	0.1875786181696455
1105	82-83	0.1449843645302522
1105	84-85	0.15849471719683095
1105	86-87	0.196614254499913
1105	88-89	0.15172476611103747
1105	90-91	0.14961472751135574
1105	92-93	0.16607457796769154
1105	94-95	0.11807730279809192
1105	96-97	0.0850790757961093
1105	98-99	0.10791376988374424
1105	100	0.1626855480807805
1106	1	0.13643610509231507
1106	2	0.12043907211084814
1106	3	0.16373261243219872
1106	4	0.09782110422082013
1106	5	0.08794246599700983
1106	6	0.053422910974688875
1106	7	0.06011466894219808
1106	8	0.049436876216219616
1106	9	0.07214138906751799
1106	10-11	0.09955672155958695
1106	12-13	0.06268261356960636
1106	14-15	0.1525774226449954
1106	16-17	0.15483115486593846
1106	18-19	0.15890418492693215
1106	20-21	0.12463834063658652
1106	22-23	0.16953006478589572
1106	24-25	0.18622223748428723
1106	26-27	0.16884024272042808
1106	28-29	0.19278307084110935
1106	30-31	0.17343836165212423
1106	32-33	0.17371470875630024
1106	34-35	0.18264538732518787
1106	36-37	0.19011311236440775
1106	38-39	0.21750677424474674
1106	40-41	0.2249115237330983
1106	42-43	0.23931191945248997
1106	44-45	0.22771041740471532
1106	46-47	0.23544022345885907
1106	48-49	0.22106607678048107
1106	50-51	0.24255880285183906
1106	52-53	0.2612239301460946
1106	54-55	0.2227603931463804
1106	56-57	0.2300443481315142
1106	58-59	0.1735486750055273
1106	60-61	0.17206433424708933
1106	62-63	0.18579995495756663
1106	64-65	0.20672756182423768
1106	66-67	0.25216437329358854
1106	68-69	0.24247692726407877
1106	70-71	0.22694176988925108
1106	72-73	0.19150794028109175
1106	74-75	0.2036715349249576
1106	76-77	0.14141162743358393
1106	78-79	0.17518030672243157
1106	80-81	0.17871063886725125
1106	82-83	0.18429579950191055
1106	84-85	0.15214006815213565
1106	86-87	0.20270025535280922
1106	88-89	0.19789679742117983
1106	90-91	0.21093783864005644
1106	92-93	0.11005336054351744
1106	94-95	0.10115445065677875
1106	96-97	0.08461152691615581
1106	98-99	0.10923921165123573
1106	100	0.15746361706802503
1107	1	0.021191422756700717
1107	2	-0.006452780482383957
1107	3	0.05285073532798634
1107	4	0.03959260900583672
1107	5	0.0587550118396436
1107	6	0.051904345036234645
1107	7	0.08507052841679297
1107	8	0.05279756633139954
1107	9	0.021024145958072893
1107	10-11	0.04644899317653284
1107	12-13	0.0620554129798947
1107	14-15	0.11084839509552324
1107	16-17	0.09389924829111607
1107	18-19	0.10429757491219505
1107	20-21	0.09511585821582713
1107	22-23	0.10541474945800644
1107	24-25	0.08561063721852946
1107	26-27	0.09465308743759948
1107	28-29	0.1429499937624854
1107	30-31	0.1440134425566768
1107	32-33	0.09451272771676855
1107	34-35	0.13326848213630882
1107	36-37	0.14607656641235423
1107	38-39	0.15500747920175684
1107	40-41	0.1193696108173441
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2216	42-43	-0.4838057230268049
2216	44-45	-0.42085186210014314
2216	46-47	-0.4520090191236932
2216	48-49	-0.4266869932493833
2216	50-51	-0.4980096597362049
2216	52-53	-0.5339791609857585
2216	54-55	-0.549536587427518
2216	56-57	-0.5190643700567534
2216	58-59	-0.5341625188744956
2216	60-61	-0.5518802187803189
2216	62-63	-0.5460854142040503
2216	64-65	-0.5061200163657489
2216	66-67	-0.475631658715848
2216	68-69	-0.5255945776959123
2216	70-71	-0.5673461716644255
2216	72-73	-0.5227324501259076
2216	74-75	-0.512414970875021
2216	76-77	-0.5056247335207971
2216	78-79	-0.5407942561798933
2216	80-81	-0.5382203966045509
2216	82-83	-0.48842244202759133
2216	84-85	-0.5592153341580115
2216	86-87	-0.5214679990837254
2216	88-89	-0.5383407411462571
2216	90-91	-0.5478076592640875
2216	92-93	-0.5511186799558772
2216	94-95	-0.5686947009619274
2216	96-97	-0.5768635420237942
2216	98-99	-0.5833159095724341
2216	100	-0.8293496570978292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	25.0
8	77.0
9	127.0
10	178.0
11	205.0
12	171.0
13	189.0
14	358.0
15	561.0
16	1019.0
17	2046.0
18	3514.0
19	5652.0
20	8288.0
21	11788.0
22	17512.0
23	22458.0
24	23265.0
25	21524.0
26	20740.0
27	22557.0
28	27703.0
29	34532.0
30	43339.0
31	55250.0
32	71700.0
33	95525.0
34	139977.0
35	224517.0
36	433022.0
37	1008609.0
38	1378401.0
39	453011.0
40	1594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.5203445674562	18.710051619731015	13.36627728865032	22.403326524162466
2	14.353234469924834	23.12217126575572	37.407058038828275	25.117536225491172
3	22.41707318018203	25.134086497293566	30.344667924630652	22.104172397893752
4	11.792482072612337	17.075237393193646	40.71233940906216	30.41994112513186
5	11.956452748387896	39.97283808133943	35.05086682180841	13.019842348464255
6	28.758019565657516	38.290247491327776	17.478245581065543	15.473487361949164
7	25.38666912059398	33.41144732875079	23.012082766770757	18.189800783884476
8	24.75943446029918	31.45497351212127	20.75036397222284	23.03522805535671
9	24.149932339428435	18.249901439324887	22.636820137180962	34.96334608406572
10-11	25.05371193547981	24.79103683892909	28.331108655031823	21.82414257055927
12-13	28.330890707593	23.27072026300928	27.081046903257487	21.31734212614023
14-15	23.424926584983975	25.15059870788603	28.446134694714253	22.978340012415742
16-17	21.102760764424	27.91312179193478	28.547603110933313	22.436514332707905
18-19	20.58144259894087	29.941558605097647	28.376344856779472	21.10065393918201
20-21	22.888852133802292	26.25311301591791	27.30937106181086	23.548663788468936
22-23	21.595188950093483	26.630968270168342	27.84122576333837	23.9326170163998
24-25	23.921063312277997	26.634448384718883	27.26041764541211	22.18407065759101
26-27	23.160099829613536	24.677716278930102	30.48910311238629	21.673080779070073
28-29	21.048850635601557	29.367701558167347	28.17845881802944	21.404988988201655
30-31	22.434542402835838	25.926550619952543	27.942713145086163	23.69619383212546
32-33	22.614630354340623	26.788122130305908	28.536988782765366	22.0602587325881
34-35	24.085572766529168	26.0100652047907	28.48133536931562	21.42302665936451
36-37	23.093761082889998	25.01392153256367	30.301161586765325	21.591155797781006
38-39	22.272590008773665	27.13237240346849	29.278457442879755	21.316580144878085
40-41	22.244020002564554	27.433794085563406	28.287858887934615	22.034327023937426
42-43	21.818574346144565	27.450358834511608	26.47561921974093	24.255447599602896
44-45	23.04896788536766	25.516741433190038	27.376014082397475	24.058276599044827
46-47	24.487237622209886	27.30158999230522	27.500128649950444	20.71104373553445
48-49	23.67232275983185	26.77476279304724	27.71590185622114	21.837012590899768
50-51	20.429408890764332	27.46206922438443	29.89845370904593	22.210068175805308
52-53	22.52532915030274	28.689422692133753	25.96798673893736	22.81726141862615
54-55	21.70992814515253	26.458138312499024	27.286861071789097	24.545072470559344
56-57	23.782236275404156	25.299844311258333	27.357858648958818	23.560060764378687
58-59	25.632598103319676	25.776907578003154	26.58614493427489	22.004349384402275
60-61	23.095576583598376	26.870901219767212	29.321284796760562	20.712237399873853
62-63	21.652886978139854	27.099300695236362	28.500913089521518	22.746899237102262
64-65	20.940188482329646	29.69454596619504	26.736354925771565	22.628910625703746
66-67	23.046749986831756	26.330152975629368	25.952566475409288	24.670530562129585
68-69	22.104091550134	26.671136374066318	26.933107079118056	24.291664996681625
70-71	24.11038942325971	26.534588991086515	26.364647525538444	22.990374060115325
72-73	23.377538454459483	24.797535136653643	29.526897839430305	22.29802856945657
74-75	21.729271905779378	29.554472721319687	26.622198941366875	22.094056431534064
76-77	22.515675810768528	26.123969920267204	26.571310529746988	24.789043739217277
78-79	22.90969152514175	26.863598591222715	27.258583669550745	22.968126214084794
80-81	24.149565757732688	25.983775744596503	27.181700060867897	22.68495843680291
82-83	23.11382518018835	25.272331840552287	29.200841084808577	22.413001894450783
84-85	22.219512676496667	27.473477899571453	27.88360679497563	22.42340262895625
86-87	22.132337716903162	27.956391128568438	26.9152559611666	22.996015193361803
88-89	21.75597927848603	27.70876499118038	25.51657275535498	25.018682974978617
90-91	23.05404976709889	25.52324954866707	26.384977605895237	25.037723078338804
92-93	24.381017165540282	27.31967513239096	26.69923931500573	21.600068387063025
94-95	23.002865645921823	26.69502889680684	27.519802646148605	22.782302811122733
96-97	20.573242453786513	27.055336073668425	29.666718489900042	22.704702982645028
98-99	22.452931578185186	28.192620066672898	26.08812049360999	23.266327861531927
100	22.394654544204094	24.444073662752146	26.015095604364202	27.146176188679554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	494.0
1	323.5
2	388.0
3	661.5
4	445.0
5	294.5
6	579.0
7	1301.0
8	2432.0
9	3024.5
10	2278.0
11	1434.0
12	1418.5
13	1646.0
14	1886.5
15	2207.5
16	2604.5
17	3086.5
18	3686.0
19	4366.0
20	5122.0
21	6098.5
22	7579.0
23	9291.5
24	11714.5
25	15203.5
26	19331.5
27	23345.5
28	27803.5
29	33523.0
30	40091.5
31	47160.0
32	54891.5
33	62072.5
34	68188.5
35	75884.5
36	83557.0
37	90995.5
38	95835.0
39	98049.5
40	100793.5
41	104089.0
42	107092.0
43	112637.0
44	125651.5
45	139016.5
46	146366.0
47	218799.5
48	379524.0
49	375246.0
50	239030.0
51	172211.0
52	135465.0
53	120633.5
54	112932.5
55	100951.5
56	89546.0
57	79654.5
58	69354.0
59	62337.5
60	53511.0
61	42084.5
62	32622.0
63	25630.0
64	18895.0
65	13059.0
66	9561.0
67	7296.0
68	5609.0
69	4194.5
70	3231.0
71	2594.0
72	2120.5
73	1969.0
74	1670.5
75	1246.5
76	945.5
77	663.5
78	435.0
79	260.5
80	180.5
81	114.5
82	63.5
83	41.0
84	26.5
85	10.0
86	7.5
87	6.0
88	3.0
89	2.0
90	2.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004867492800469604
2	0.08412771138722092
3	2.905965851026629E-4
4	0.0
5	0.03869777858283795
6	0.003874621134702172
7	2.421638209188858E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.632457313783286E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.175177433431588E-4
24-25	0.0
26-27	0.0
28-29	3.632457313783286E-5
30-31	1.6951467464322006E-4
32-33	0.001840445038983532
34-35	0.0014893074986511473
36-37	0.001004979856813376
38-39	7.99140609032323E-4
40-41	0.001586173027018702
42-43	6.175177433431588E-4
44-45	6.175177433431588E-4
46-47	3.269211582404958E-4
48-49	6.054095522972145E-4
50-51	9.323307105377103E-4
52-53	0.0012955764419160388
54-55	0.0016830385553862563
56-57	0.0010413044299512088
58-59	0.002809100322659075
60-61	0.0019978515225808078
62-63	0.0011866027225025404
64-65	0.004589004406412886
66-67	0.004443706113861554
68-69	0.003814080179472451
70-71	0.0017677958927078664
72-73	0.004867492800469604
74-75	0.003608240931691398
76-77	0.0034871590212319555
78-79	9.565470926295988E-4
80-81	3.632457313783286E-5
82-83	1.6951467464322006E-4
84-85	6.054095522972144E-5
86-87	2.421638209188858E-5
88-89	1.9373105673510863E-4
90-91	2.421638209188858E-5
92-93	0.0
94-95	1.210819104594429E-5
96-97	3.390293492864401E-4
98-99	5.327604060215487E-4
100	5.327604060215487E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4129436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.60833788512114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42476976055697	38.28963190690921
2	15.433112278701794	16.54686995311315
3	5.898125296942161	9.485660814217674
4	2.824313826549034	6.0562707962904
5	1.4860319578577004	3.9831851652461845
6	0.8790413984594659	2.827436898217467
7	0.5590942781530303	2.098048048100588
8	0.37749556587039257	1.6189527876252003
9	0.25151595731489046	1.2135017180912715
>10	0.8463040785082867	6.693793512201236
>50	0.012330009649680633	0.44390906505015665
>100	0.0057093682225965595	0.5549211020719933
>500	9.147613635471853E-4	0.36775624421490216
>1k	5.227207791478221E-4	0.7385599018041573
>5k	2.6136038957391103E-4	1.102813371989665
>10k+	4.5738068175434435E-4	7.978688714856749
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	108290	2.622392016730614	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	28215	0.6832652207226362	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	26256	0.6358253282046266	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	21601	0.5230980695668852	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	17839	0.4319960401372004	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	15337	0.37140665214329516	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10244	0.24807261814930656	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	9366	0.2268106346726284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9328	0.22589041215313665	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	7543	0.18266417011911554	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	5247	0.12706335683613937	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4988	0.12079131387434024	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	4750	0.11502781493647074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.264914627566574E-5
2	0.0	0.0	0.0	0.0	7.264914627566574E-5
3	0.0	0.0	0.0	0.0	7.264914627566574E-5
4	0.0	0.0	0.0	0.0	7.264914627566574E-5
5	0.0	0.0	0.0	0.0	7.264914627566574E-5
6	0.0	0.0	0.0	0.0	1.4529829255133147E-4
7	0.0	0.0	0.0	0.0	1.4529829255133147E-4
8	0.0	0.0	0.0	0.0	1.4529829255133147E-4
9	0.0	0.0	0.0	0.0	1.6951467464322004E-4
10-11	0.0	0.0	0.0	2.421638209188858E-5	1.6951467464322004E-4
12-13	0.0	0.0	0.0	2.421638209188858E-5	4.964358328837158E-4
14-15	0.0	0.0	0.0	3.632457313783287E-5	7.022750806647687E-4
16-17	0.0	0.0	0.0	1.2108191045944288E-4	8.838979463539331E-4
18-19	0.0	0.0	0.0	2.542720119648301E-4	9.444389015836545E-4
20-21	0.0	0.0	0.0	3.8746211347021726E-4	0.0010413044299512088
22-23	0.0	0.0	0.0	6.417341254350473E-4	0.0011139535762268746
24-25	0.0	0.0	0.0	0.0011139535762268746	0.0011260617672728189
26-27	0.0	0.0	0.0	0.001840445038983532	0.001174494531456596
28-29	0.0	0.0	0.0	0.004492138878045331	0.0011866027225025404
30-31	0.0	0.0	0.0	0.011672296168290294	0.001210819104594429
32-33	0.0	0.0	0.0	0.03033101857009044	0.0013319010150538717
34-35	0.0	0.0	0.0	0.056630009521881436	0.0014529829255133147
36-37	0.0	1.210819104594429E-5	0.0	0.09448021473150328	0.0014529829255133147
38-39	0.0	2.421638209188858E-5	0.0	0.1465938689932475	0.0014771993076052032
40-41	0.0	2.421638209188858E-5	0.0	0.20495534983469899	0.0014771993076052032
42-43	0.0	2.421638209188858E-5	0.0	0.2607620023654562	0.0015014156896970918
44-45	0.0	2.421638209188858E-5	0.0	0.32772029884952814	0.001549848453880869
46-47	0.0	2.421638209188858E-5	0.0	0.4099228078604439	0.0016103894091105905
48-49	0.0	2.421638209188858E-5	0.0	0.48660398175440905	0.001719363128524089
50-51	0.0	2.421638209188858E-5	0.0	0.5682495139772115	0.0017435795106159775
52-53	0.0	2.421638209188858E-5	0.0	0.6493017448387626	0.0017920122747997547
54-55	0.0	2.421638209188858E-5	0.0	0.7378489459577531	0.0017920122747997547
56-57	0.0	2.421638209188858E-5	0.0	0.8501160933357486	0.0018888778031673089
58-59	0.0	2.421638209188858E-5	0.0	0.9604943629105767	0.0019736351404889187
60-61	0.0	2.421638209188858E-5	0.0	1.0735606508976043	0.002058392477810529
62-63	0.0	2.421638209188858E-5	0.0	1.18755926959517	0.0022036907703618606
64-65	0.0	2.421638209188858E-5	0.0	1.3075998756246614	0.0022400153434996937
66-67	0.0	2.421638209188858E-5	0.0	1.4446888146468428	0.0022763399166375264
68-69	0.0	2.421638209188858E-5	0.0	1.5940070266254276	0.002300556298729415
70-71	0.0	2.421638209188858E-5	0.0	1.7516798904257143	0.002336880871867248
72-73	0.0	2.421638209188858E-5	0.0	1.896263799705335	0.002482179164418579
74-75	0.0	2.421638209188858E-5	0.0	2.0455093625376444	0.0025911528838320776
76-77	0.0	2.421638209188858E-5	0.0	2.2010269683317527	0.002615369265923966
78-79	0.0	2.421638209188858E-5	0.0	2.4032942997542523	0.0027122347942915206
80-81	0.0	2.421638209188858E-5	0.0	2.6125722737923534	0.002796992131613131
82-83	0.0	2.421638209188858E-5	0.0	2.8071387957096317	0.0030028313793941837
84-85	0.0	2.421638209188858E-5	0.0	2.9985329715728737	0.003039155952532017
86-87	0.0	2.421638209188858E-5	0.0	3.195327400642606	0.0031723460540374034
88	0.0	2.421638209188858E-5	0.0	3.3603136118346426	0.0032934279644968464
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	11385	0.0	21.81605	12-13
GAGTACG	1785	0.0	21.093437	1
GTACGCA	1740	0.0	20.535593	3
CTAACGC	740	0.0	20.331116	3
TACGCAG	1800	0.0	20.112274	4
TAACGCC	750	0.0	20.060034	4
CTAGTAC	1805	0.0	20.05656	3
AGTACTT	12040	0.0	19.73144	12-13
TAGTACT	1985	0.0	19.18525	4
GTACTTT	12905	0.0	19.100801	14-15
CATGGGG	6785	0.0	18.847858	4
TCTAACG	725	0.0	18.82498	2
TACCTGG	3720	0.0	17.711676	2
AGTACGC	2060	0.0	17.591305	2
CGCAGTG	2065	0.0	17.524698	6
GTATAAG	1550	0.0	17.307676	1
CGATAAT	750	0.0	16.920223	66-67
GTACCTG	4045	0.0	16.754812	1
ACTTTTT	15250	0.0	16.502851	16-17
CGCCTAC	860	0.0	16.394075	7
>>END_MODULE