FastQCFastQC Report
Mon 16 Jan 2017
ERR1146552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025781
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT838702.0833224658768077No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC207110.5144591819574885No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC158400.3934640259865105No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA152380.3785104058069726No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129340.3212792747543893No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59690.1482693668632248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20800.027.5802821
TAGGCAT27850.024.1292175
GCATCGT22000.023.2828568
TAGTACT29950.023.0649724
CTAACGC8250.022.2148343
CTAGTAC33400.022.0894873
CTATTGA27250.022.0737389
GGGCACC29200.021.89200894
ACGTTAA4600.021.4532898
CTGTGCG31550.021.4484339
TTTAGGG28550.021.3978252
TCTAACG8600.021.3107432
GCTATTG28550.021.0686288
CGTTAAC5150.020.9871279
GGGCTAT29350.020.9746936
CTAGGCA30650.020.8516714
CATGGGG85400.020.8001444
GACGTTA5000.020.6768867
CTCGTCG17750.020.3856629
TAACGCC9350.020.1039224
Produced by FastQC (version 0.11.5)