##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146552_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4025781
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39718802388903	31.0	31.0	34.0	30.0	34.0
2	31.662048929139463	33.0	31.0	34.0	30.0	34.0
3	31.794090140521803	33.0	31.0	34.0	30.0	34.0
4	35.273400366289174	37.0	35.0	37.0	33.0	37.0
5	35.24986629923486	37.0	35.0	37.0	33.0	37.0
6	35.40433197931035	37.0	35.0	37.0	33.0	37.0
7	35.360534018119715	37.0	35.0	37.0	33.0	37.0
8	35.31746883399768	37.0	35.0	37.0	33.0	37.0
9	36.95791027877572	39.0	38.0	39.0	33.0	39.0
10-11	36.93408198309844	39.0	38.0	39.0	33.0	39.0
12-13	36.89491318578929	39.0	38.0	39.0	33.0	39.0
14-15	38.25837756698638	40.0	38.0	41.0	33.0	41.0
16-17	38.2934204568008	40.0	38.0	41.0	33.0	41.0
18-19	38.34274392968719	40.0	38.5	41.0	33.5	41.0
20-21	38.40402346774452	40.0	39.0	41.0	34.0	41.0
22-23	38.402199722240226	40.0	39.0	41.0	34.0	41.0
24-25	38.41078190790806	40.5	38.5	41.0	33.5	41.0
26-27	38.37979897565218	40.0	38.0	41.0	33.0	41.0
28-29	38.35109957546126	40.0	38.0	41.0	33.0	41.0
30-31	38.361981066530944	40.0	38.0	41.0	33.0	41.0
32-33	38.32558837651626	40.0	38.0	41.0	33.0	41.0
34-35	38.20555862328328	40.0	38.0	41.0	33.0	41.0
36-37	38.09220372394822	40.0	38.0	41.0	33.0	41.0
38-39	38.027247756398076	40.0	38.0	41.0	33.0	41.0
40-41	38.01294804660263	40.0	38.0	41.0	33.0	41.0
42-43	37.87611248599961	40.0	38.0	41.0	32.0	41.0
44-45	37.714012883462864	40.0	37.0	41.0	32.0	41.0
46-47	37.53968186545667	40.0	37.0	41.0	31.0	41.0
48-49	37.35976758298576	40.0	37.0	41.0	31.0	41.0
50-51	37.19210992848345	40.0	36.0	41.0	30.5	41.0
52-53	37.11347686324716	39.5	36.0	41.0	31.0	41.0
54-55	36.91193199530724	39.0	35.0	41.0	30.0	41.0
56-57	36.63304772415589	39.0	35.0	41.0	30.0	41.0
58-59	36.35409849666438	39.0	35.0	40.5	29.5	41.0
60-61	36.06765618894818	38.0	35.0	40.0	29.0	41.0
62-63	35.86447797334232	37.5	35.0	40.0	29.0	41.0
64-65	35.62994323337509	37.0	34.5	40.0	29.0	41.0
66-67	35.28244767909631	36.5	34.0	39.5	28.5	41.0
68-69	34.897746673254204	36.0	34.0	39.0	28.0	41.0
70-71	34.5185257220897	35.0	34.0	39.0	28.0	41.0
72-73	34.1921238388278	35.0	33.5	37.5	27.5	40.0
74-75	33.93668731607606	35.0	33.0	37.0	27.0	39.5
76-77	33.66162379424017	35.0	33.0	36.5	27.0	39.0
78-79	33.361012558805356	35.0	33.0	36.0	26.5	39.0
80-81	33.02244682460372	35.0	33.0	36.0	26.0	38.0
82-83	32.78466153523999	35.0	32.5	35.5	26.0	37.0
84-85	32.61564501397368	35.0	32.5	35.0	26.0	37.0
86-87	32.54936222313136	35.0	33.0	35.0	26.0	36.0
88-89	32.42372039114895	35.0	33.0	35.0	25.5	36.0
90-91	32.180143306354715	35.0	32.0	35.0	25.0	36.0
92-93	32.02522193830215	35.0	32.0	35.0	25.0	36.0
94-95	31.935238777270797	35.0	32.0	35.0	25.0	35.0
96-97	31.867525083952657	35.0	32.0	35.0	25.0	35.0
98-99	31.894536240297224	35.0	32.0	35.0	25.0	35.0
100	30.870296720065	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19046309724927468
1101	2	0.2804657253449534
1101	3	0.3064755495930349
1101	4	0.27250949411426006
1101	5	0.19265238074333269
1101	6	0.27515433192007066
1101	7	0.2708807650672327
1101	8	0.40894304553971494
1101	9	0.45766523208852306
1101	10-11	0.48449333448836285
1101	12-13	0.4141313148130621
1101	14-15	0.48996826175540065
1101	16-17	0.5109787215867883
1101	18-19	0.4426205687306819
1101	20-21	0.4556542074166643
1101	22-23	0.43987697852489305
1101	24-25	0.4188701897698621
1101	26-27	0.4336982925804307
1101	28-29	0.4898225297964345
1101	30-31	0.5006725437997872
1101	32-33	0.5128184014198922
1101	34-35	0.5370414185357362
1101	36-37	0.4691098261994142
1101	38-39	0.49226047697694497
1101	40-41	0.48175225837817237
1101	42-43	0.5232222696510007
1101	44-45	0.46649954849719677
1101	46-47	0.4522171939225288
1101	48-49	0.530476029197338
1101	50-51	0.537351575203445
1101	52-53	0.5269136388986198
1101	54-55	0.6050997893050152
1101	56-57	0.5632571375575495
1101	58-59	0.46374095455442443
1101	60-61	0.4783928953874934
1101	62-63	0.4999733484343736
1101	64-65	0.45342685350866674
1101	66-67	0.4900536579658592
1101	68-69	0.41762775477126013
1101	70-71	0.4476456194592089
1101	72-73	0.4048870038676071
1101	74-75	0.39654788540521224
1101	76-77	0.46409537042167415
1101	78-79	0.46508751564122974
1101	80-81	0.44440261797240055
1101	82-83	0.45066079503547485
1101	84-85	0.4374678297822925
1101	86-87	0.4645287479906557
1101	88-89	0.36348246153890074
1101	90-91	0.40094912930349835
1101	92-93	0.4266265317300437
1101	94-95	0.356397016091357
1101	96-97	0.37250559962667396
1101	98-99	0.36213167680989145
1101	100	0.3781892032110221
1102	1	0.13214236232748533
1102	2	0.2079754174709727
1102	3	0.2214005752437096
1102	4	0.1630687731879874
1102	5	0.1512921716878708
1102	6	0.17179611890055213
1102	7	0.1885490619509369
1102	8	0.2633632781625934
1102	9	0.3025364948839453
1102	10-11	0.2870519772135651
1102	12-13	0.26679403105059407
1102	14-15	0.35714552994554793
1102	16-17	0.3832809183748296
1102	18-19	0.34823816357481263
1102	20-21	0.307505371809242
1102	22-23	0.3142446034710886
1102	24-25	0.30660746911613046
1102	26-27	0.3237580069393715
1102	28-29	0.35627682959113116
1102	30-31	0.3166384824075621
1102	32-33	0.3412608983869063
1102	34-35	0.36928344526578627
1102	36-37	0.4054026914564659
1102	38-39	0.3931048650876008
1102	40-41	0.4208415041843878
1102	42-43	0.4049504160473205
1102	44-45	0.34468524608241324
1102	46-47	0.34000552638188
1102	48-49	0.4321150008468564
1102	50-51	0.32165304302120035
1102	52-53	0.24532602564350725
1102	54-55	0.35337808049137465
1102	56-57	0.4216700904066286
1102	58-59	0.40354572196674354
1102	60-61	0.3809101991695911
1102	62-63	0.4068143655576151
1102	64-65	0.40707629526992406
1102	66-67	0.4023543201966078
1102	68-69	0.41795880345925696
1102	70-71	0.40604070371870193
1102	72-73	0.3310694447236102
1102	74-75	0.3225089973079349
1102	76-77	0.3683292888207177
1102	78-79	0.37584541429509244
1102	80-81	0.3394041106646526
1102	82-83	0.3475128334069524
1102	84-85	0.3191951981558532
1102	86-87	0.3285256258223228
1102	88-89	0.316338616465913
1102	90-91	0.413771296491106
1102	92-93	0.3874367468577802
1102	94-95	0.3644960663923129
1102	96-97	0.3641692894300341
1102	98-99	0.32689015371991914
1102	100	0.25086203522649697
1103	1	0.17554306464237257
1103	2	0.16324286615030204
1103	3	0.24115774934854173
1103	4	0.18180949614077235
1103	5	0.11888558600667665
1103	6	0.16925640487287552
1103	7	0.1889044827047286
1103	8	0.25799977452312106
1103	9	0.2997968838717284
1103	10-11	0.3602777881558268
1103	12-13	0.33883862265170706
1103	14-15	0.41108965623694615
1103	16-17	0.2840030271988354
1103	18-19	0.25237842382806974
1103	20-21	0.2788354893895644
1103	22-23	0.31462949761051817
1103	24-25	0.3093564333060712
1103	26-27	0.3240775375925722
1103	28-29	0.3302692313305542
1103	30-31	0.3893310534612553
1103	32-33	0.3494121927796954
1103	34-35	0.3787317861425592
1103	36-37	0.40549067658187
1103	38-39	0.3395037611516116
1103	40-41	0.3139755756792013
1103	42-43	0.4005846572044405
1103	44-45	0.3729719744887561
1103	46-47	0.3184218724303136
1103	48-49	0.3146431125838305
1103	50-51	0.2958028174138505
1103	52-53	0.2897233851761811
1103	54-55	0.31085548930441576
1103	56-57	0.25951089410126116
1103	58-59	0.29253329050595767
1103	60-61	0.3146447838276032
1103	62-63	0.2783756588889261
1103	64-65	0.29879586176971173
1103	66-67	0.27348058200553993
1103	68-69	0.24960878231672723
1103	70-71	0.24531645888991704
1103	72-73	0.2632800052008264
1103	74-75	0.313269906593419
1103	76-77	0.2965552868265391
1103	78-79	0.3289886040506218
1103	80-81	0.28740729422533917
1103	82-83	0.28123382332988456
1103	84-85	0.34014664254750926
1103	86-87	0.2580670503939544
1103	88-89	0.2502866928435239
1103	90-91	0.3192876702075438
1103	92-93	0.3341336395146257
1103	94-95	0.23912661587192474
1103	96-97	0.20307341892326392
1103	98-99	0.17811872624228542
1103	100	0.2602332667659333
1104	1	-0.036978092874381474
1104	2	0.0785610561178558
1104	3	0.1203435575703864
1104	4	0.09373784671595331
1104	5	-0.011910706720627218
1104	6	0.01898662889458791
1104	7	0.08163765352468033
1104	8	0.07632555068848745
1104	9	0.0789105609883336
1104	10-11	0.10417587565339659
1104	12-13	0.0714260737209571
1104	14-15	0.17034591022014922
1104	16-17	0.1205543392000692
1104	18-19	0.1343025294601503
1104	20-21	0.08143367411024371
1104	22-23	0.12610616191447832
1104	24-25	0.10019336378684329
1104	26-27	0.15054050746952896
1104	28-29	0.14136615567205268
1104	30-31	0.12093400969419577
1104	32-33	0.16331180770414733
1104	34-35	0.19633791442384307
1104	36-37	0.18772524701674342
1104	38-39	0.20260350636095126
1104	40-41	0.14873298427080073
1104	42-43	0.1284011840036854
1104	44-45	0.12315685496508166
1104	46-47	0.1345061009177826
1104	48-49	0.1890506718752718
1104	50-51	0.2225854784777539
1104	52-53	0.12379755078984545
1104	54-55	0.18548767711462233
1104	56-57	0.16693560331224688
1104	58-59	0.28589476263098135
1104	60-61	0.26809433264195803
1104	62-63	0.283370875684092
1104	64-65	0.21726106855764016
1104	66-67	0.17853772914035915
1104	68-69	0.21994441169589862
1104	70-71	0.21197331118364104
1104	72-73	0.13236981064440556
1104	74-75	0.14496737110509628
1104	76-77	0.09703041201102991
1104	78-79	0.13527344490658777
1104	80-81	0.16151726398859978
1104	82-83	0.1713671093015705
1104	84-85	0.1341354449561294
1104	86-87	0.0890254465916911
1104	88-89	0.11606622217831841
1104	90-91	0.16075399733252027
1104	92-93	0.16191481306768907
1104	94-95	0.1685265158533511
1104	96-97	0.18111519582943103
1104	98-99	0.18492115179349966
1104	100	0.07111547439976107
1105	1	-0.008911897956817683
1105	2	0.14759599173296323
1105	3	0.15343452166025173
1105	4	0.1920698323214367
1105	5	0.06996471076136146
1105	6	0.13344990942512425
1105	7	0.09256540099178068
1105	8	0.0799200935755664
1105	9	0.17416168634425588
1105	10-11	0.21295580937331948
1105	12-13	0.1622373165465376
1105	14-15	0.21608204018698274
1105	16-17	0.149558019187765
1105	18-19	0.11567684499879505
1105	20-21	0.20391457306183014
1105	22-23	0.20146893958474976
1105	24-25	0.2176229286906164
1105	26-27	0.24292473308487672
1105	28-29	0.284273701414655
1105	30-31	0.2111859961512721
1105	32-33	0.211434293545409
1105	34-35	0.22336535459378837
1105	36-37	0.22831184635658275
1105	38-39	0.23233511462797907
1105	40-41	0.24181903267064797
1105	42-43	0.2449529674842097
1105	44-45	0.22374262551881685
1105	46-47	0.21272389779397116
1105	48-49	0.14353026073654718
1105	50-51	0.14117764715879133
1105	52-53	0.13682012165136825
1105	54-55	0.23102324781558536
1105	56-57	0.21466764726223886
1105	58-59	0.1891098602496939
1105	60-61	0.20146368458826203
1105	62-63	0.20662994015186342
1105	64-65	0.22520222385758615
1105	66-67	0.2593561275327687
1105	68-69	0.2529775413430073
1105	70-71	0.172991043404366
1105	72-73	0.23868743227161815
1105	74-75	0.25573693004515974
1105	76-77	0.20618086000539648
1105	78-79	0.16716689862426648
1105	80-81	0.21081128143290329
1105	82-83	0.22815809261571474
1105	84-85	0.19923767014542193
1105	86-87	0.2505300051053183
1105	88-89	0.21422145710939589
1105	90-91	0.3121934460807836
1105	92-93	0.2848746242920086
1105	94-95	0.2775775950557744
1105	96-97	0.2559405773149841
1105	98-99	0.22448103532751063
1105	100	0.12837645166529654
1106	1	0.09115869444582358
1106	2	0.13213952004729634
1106	3	0.13873675898611282
1106	4	0.1610207782367823
1106	5	0.1281714292679368
1106	6	0.1797368704877158
1106	7	0.10129265880939897
1106	8	0.17771170326390973
1106	9	0.2057157749020746
1106	10-11	0.21865473908860622
1106	12-13	0.23951340750937788
1106	14-15	0.2321761100043318
1106	16-17	0.18977010781507886
1106	18-19	0.17890344264338154
1106	20-21	0.20272618946192011
1106	22-23	0.136940843363611
1106	24-25	0.1831537773060461
1106	26-27	0.1543451103015343
1106	28-29	0.1893850705188882
1106	30-31	0.24606265303167874
1106	32-33	0.21738770941329477
1106	34-35	0.18833709118011654
1106	36-37	0.19977744371247041
1106	38-39	0.20459824320967357
1106	40-41	0.2028221710326008
1106	42-43	0.13841116436113765
1106	44-45	0.08992309918649966
1106	46-47	0.15601788406915773
1106	48-49	0.1417599497295754
1106	50-51	0.13902464500976208
1106	52-53	0.09912426260339657
1106	54-55	0.16459492219019012
1106	56-57	0.06383793402899585
1106	58-59	0.1718166905185612
1106	60-61	0.20600570330010726
1106	62-63	0.12762587739135967
1106	64-65	0.11299365499079528
1106	66-67	0.08957096843413126
1106	68-69	0.0912993713972412
1106	70-71	0.04309872297041295
1106	72-73	0.06410745165656806
1106	74-75	-0.05249721801659746
1106	76-77	0.05867278324135583
1106	78-79	0.055016585623732794
1106	80-81	0.0886484894125914
1106	82-83	0.06373773367540991
1106	84-85	0.11600687074387395
1106	86-87	0.08150701189366316
1106	88-89	0.040643173790137155
1106	90-91	0.160529363353362
1106	92-93	0.040835855704393964
1106	94-95	0.019111707081869156
1106	96-97	0.042921178772832747
1106	98-99	-8.575505322028221E-5
1106	100	-0.026880872732345296
1107	1	-0.008657681117032467
1107	2	0.05093291091211327
1107	3	0.12822480473620956
1107	4	0.12287784929603163
1107	5	0.12156642047732902
1107	6	0.12753134881906192
1107	7	0.07187641111832477
1107	8	0.1510222935326695
1107	9	0.21363268072946795
1107	10-11	0.21322259342976224
1107	12-13	0.18755451309493054
1107	14-15	0.16911146949652078
1107	16-17	0.17931694885347582
1107	18-19	0.1475952413932191
1107	20-21	0.20034009758154525
1107	22-23	0.16331187762286703
1107	24-25	0.1685436987330533
1107	26-27	0.1782902811016953
1107	28-29	0.19741801662821246
1107	30-31	0.1937495814800414
1107	32-33	0.13903078822552573
1107	34-35	0.23011454837691758
1107	36-37	0.28596239682460833
1107	38-39	0.2382248304796164
1107	40-41	0.2543457948577341
1107	42-43	0.2250645093117356
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2216	64-65	-0.8600779527607827
2216	66-67	-0.8903326535907752
2216	68-69	-0.9489346630820066
2216	70-71	-0.9987421537624073
2216	72-73	-0.9358285526996326
2216	74-75	-0.918089861055968
2216	76-77	-0.8046347922469934
2216	78-79	-0.8204464241979679
2216	80-81	-0.893617980308143
2216	82-83	-0.9106898534060548
2216	84-85	-0.7549705531995805
2216	86-87	-0.6762307040755751
2216	88-89	-0.8215730269507517
2216	90-91	-0.8064312120097803
2216	92-93	-0.7821645428358472
2216	94-95	-0.8053430580659011
2216	96-97	-0.7664094503562957
2216	98-99	-0.6933765909948413
2216	100	-0.7163066823950537
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	118.0
1	81.0
2	55.0
3	51.0
4	41.0
5	57.0
6	125.0
7	444.0
8	1429.0
9	2056.0
10	2346.0
11	3300.0
12	4976.0
13	6396.0
14	7767.0
15	8585.0
16	9304.0
17	10164.0
18	11126.0
19	12288.0
20	14033.0
21	15534.0
22	18115.0
23	21755.0
24	26643.0
25	33934.0
26	38130.0
27	38516.0
28	42456.0
29	52630.0
30	63015.0
31	73724.0
32	94669.0
33	129615.0
34	197256.0
35	308057.0
36	536950.0
37	978007.0
38	966466.0
39	295121.0
40	446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14730796227548	19.082950394604193	12.86790950251437	22.901832140605954
2	14.504374517744756	22.235512965554864	37.45875522127473	25.801357295425646
3	21.9102389469372	25.5282418371659	29.654407250961235	22.907111964935666
4	12.006774050824262	16.65162453752595	40.58470780633091	30.756893605318876
5	12.445770839735582	39.725569333206465	34.85482621204642	12.973833615011532
6	29.32415760679191	37.43829588066156	17.875400781763553	15.362145730782972
7	25.86980898010609	32.71182769309037	23.359409427635857	18.058953899167687
8	25.179268141816713	31.462260659082748	20.83594375305239	22.52252744604815
9	24.578867377443636	17.87189718228056	22.568559968639562	34.98067547163624
10-11	25.358709220650976	24.883162856557302	27.78062216260193	21.977505760189793
12-13	28.031934859385416	23.368845216267168	26.431962483235033	22.167257441112387
14-15	23.801571794645664	24.85427920905962	27.016322865053468	24.327826131241252
16-17	21.891494679594402	27.62882044323588	27.571732253907793	22.90795262326192
18-19	21.482168133920467	30.192322119801247	26.747004173959876	21.578505572318413
20-21	23.716189729517836	26.864027079517015	25.017550710747894	24.402232480217258
22-23	22.78821580737985	26.95848436431853	25.546406754264638	24.70689307403698
24-25	24.776011956462202	27.077715389647594	24.995407523166392	23.150865130723812
26-27	24.02237807959739	25.17741379959218	27.89054852552318	22.90965959528725
28-29	22.07000020991095	29.24519126924458	26.157773791263807	22.527034729580663
30-31	23.377075378855583	26.467467228866248	25.717413724594167	24.438043667684003
32-33	23.53385494623429	27.22759720619526	26.08272354971698	23.15582429785347
34-35	24.674507923652705	26.4715213065394	26.205565313306618	22.64840545650127
36-37	24.040681547093318	25.41780548116667	27.957843180367597	22.58366979137241
38-39	23.488004420368828	27.072224906139837	27.078585149056394	22.361185524434948
40-41	22.911233965249263	27.52952292268736	26.665648863306608	22.893594248756774
42-43	22.616531937968894	27.44706199507796	24.781744478856478	25.154661588096666
44-45	23.72259758523936	25.998922880344676	25.579355579312097	24.699123955103865
46-47	24.81359856459651	27.166044686649045	26.10671154497451	21.913645203779936
48-49	24.26637654193987	26.505077928157604	26.418321292014163	22.81022423788836
50-51	21.266406150316186	27.142957777889045	28.532069092285678	23.05856697950909
52-53	22.81401085470388	28.365464474356894	25.02212374263034	23.798400928308887
54-55	22.129659839975268	26.331644655542284	26.266005144308767	25.272690360173677
56-57	24.423888870182424	25.103012219821856	26.016756049743073	24.456342860252647
58-59	25.73924076551638	25.590418521250097	25.778067741082594	22.89227297215093
60-61	23.708878830386396	26.552040325676806	27.936750711625933	21.802330132310864
62-63	22.431986840290676	26.799203857464644	27.094725804187203	23.674083498057477
64-65	21.526217564668144	29.08270920322354	25.931728587670833	23.459344644437486
66-67	23.620591993024295	26.136362930416784	24.93582971351084	25.30721536304808
68-69	22.575789786663762	26.37356971010331	26.039647472547244	25.010993030685686
70-71	24.423031261333897	26.185512417322737	25.35522661705627	24.036229704287095
72-73	23.92605893229478	24.80505368245462	28.172013459191668	23.096873926058933
74-75	22.479641577960507	28.73371304493359	25.902873233388696	22.88377214371721
76-77	22.951583897397235	26.04143810264799	25.905051390095817	25.101926609858953
78-79	23.47601935314495	26.321042488480167	26.534796312013487	23.668141846361397
80-81	24.311636777465722	25.874537298370438	26.397728262513215	23.416097661650625
82-83	23.485292765536588	24.964564473523996	28.420472589651137	23.12967017128828
84-85	23.051597467370232	27.023364865098245	26.693560420683614	23.231477246847913
86-87	22.555455706439126	27.652084264371467	26.261779534890305	23.530680494299098
88-89	22.666863130190713	26.86499129331971	25.002621446263085	25.46552413022649
90-91	23.53191559677116	25.226458126445316	25.85462325684487	25.387003019938653
92-93	24.750049293754373	26.70303864468826	26.14754431374956	22.399367747807812
94-95	23.182497314671984	26.252176684332778	26.866947510457095	23.69837849053814
96-97	21.325961277074317	26.54837284495076	28.55018131958515	23.575484558389775
98-99	22.93031282263445	27.77314364446631	25.384030503281785	23.912513029617454
100	22.783615251509975	24.36224274283079	25.447252527722203	27.406889477937035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1869.0
1	1295.0
2	734.5
3	716.0
4	642.0
5	517.5
6	424.5
7	410.0
8	434.0
9	466.0
10	413.0
11	348.5
12	336.5
13	350.0
14	398.0
15	481.5
16	546.5
17	607.5
18	754.0
19	889.5
20	1107.5
21	1555.5
22	2419.0
23	3742.0
24	5407.5
25	7540.0
26	10949.5
27	15103.5
28	18981.5
29	24578.5
30	31714.0
31	39022.0
32	47800.0
33	56013.0
34	63613.0
35	71212.5
36	78894.0
37	89946.0
38	95181.0
39	93403.5
40	91620.0
41	89616.0
42	90822.5
43	98488.0
44	109538.5
45	123153.5
46	138687.5
47	203659.0
48	338613.5
49	341192.0
50	239059.0
51	189104.0
52	153411.5
53	138225.0
54	131887.5
55	120352.5
56	110087.5
57	97285.0
58	83627.0
59	77515.0
60	67047.5
61	53260.0
62	42864.0
63	33768.5
64	24442.0
65	16518.5
66	12103.0
67	8874.0
68	6511.5
69	4951.5
70	3997.5
71	3310.5
72	2811.5
73	2465.5
74	1977.0
75	1540.0
76	1120.0
77	748.0
78	484.0
79	293.0
80	216.0
81	156.5
82	98.5
83	68.5
84	57.5
85	51.5
86	39.5
87	28.5
88	25.5
89	21.5
90	17.0
91	13.0
92	12.5
93	10.5
94	7.0
95	4.5
96	7.0
97	7.5
98	3.5
99	5.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.062323310681827955
2	0.010507277966685222
3	0.012270910911447991
4	0.010432758264793838
5	0.012469630116491683
6	0.012991268029731374
7	0.003154667380068613
8	0.006135455455723996
9	0.008122647506160915
10-11	0.007601009592921225
12-13	0.0068061327727464566
14-15	0.011078595681185837
16-17	0.010681157271098453
18-19	0.008619445518770147
20-21	0.00752648989102984
22-23	0.0029062683737639977
24-25	0.004359402560645996
26-27	0.005824956697843226
28-29	0.006570153716757072
30-31	0.0099732201031303
32-33	0.011078595681185837
34-35	0.012196391209556606
36-37	0.018928004280411675
38-39	0.01931302274018383
40-41	0.019201243187346752
42-43	0.02548573804685352
44-45	0.028777024880389666
46-47	0.0314969939994252
48-49	0.03241607032275228
50-51	0.03322336709324228
52-53	0.031261014943435826
54-55	0.037955368163345195
56-57	0.04030273877292381
58-59	0.03768212925641012
60-61	0.038402486374693505
62-63	0.03417970326751505
64-65	0.035930916261962584
66-67	0.037942948213029966
68-69	0.040749856984272116
70-71	0.040352418574184735
72-73	0.0364649741255175
74-75	0.0394085023502272
76-77	0.03879992478478089
78-79	0.03789326841176904
80-81	0.042836408637230886
82-83	0.03902348389045505
84-85	0.03543411824935336
86-87	0.03416728331719982
88-89	0.03191927231014305
90-91	0.03381952470837336
92-93	0.035707357156288436
94-95	0.039706581157792735
96-97	0.03465166137949382
98-99	0.032192511217078126
100	0.030031439862227977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4025781.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.17987235387413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56512302057094	37.342589842026904
2	14.150231963286911	14.767145952440416
3	5.781766176020539	9.050754631340961
4	2.8690648755458925	5.988297559238738
5	1.726149871187278	4.503513997110422
6	1.0493129642631545	3.285180992071001
7	0.6697371482718915	2.4462759235232476
8	0.47827399902667644	1.996502097551112
9	0.34599855631419335	1.6248744452789405
>10	1.33276402759375	10.865842134247387
>50	0.024180601665209387	0.8289016582027073
>100	0.006464068639298594	0.5336663035659104
>500	0.0	0.0
>1k	4.6636380710311066E-4	0.4977278562815238
>5k	7.772730118385178E-5	0.24209074104827374
>10k+	3.8863650591925894E-4	6.0266358660723975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	83870	2.0833224658768077	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	20711	0.5144591819574885	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	15840	0.3934640259865105	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	15238	0.3785104058069726	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	12934	0.3212792747543893	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	5969	0.1482693668632248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.2291870819599973E-4	0.0	0.0	0.0	4.967980126092304E-5
7	3.2291870819599973E-4	0.0	0.0	0.0	7.451970189138455E-5
8	3.4775860882646124E-4	0.0	0.0	0.0	9.935960252184608E-5
9	3.4775860882646124E-4	0.0	0.0	2.483990063046152E-5	9.935960252184608E-5
10-11	3.60178559141692E-4	0.0	0.0	2.483990063046152E-5	9.935960252184608E-5
12-13	3.7259850945692275E-4	0.0	0.0	2.483990063046152E-5	9.935960252184608E-5
14-15	4.4711821134830733E-4	0.0	0.0	3.725985094569228E-5	1.1177955283707685E-4
16-17	5.464778138701534E-4	0.0	0.0	4.967980126092304E-5	1.241995031523076E-4
18-19	5.464778138701534E-4	0.0	0.0	9.935960252184608E-5	1.241995031523076E-4
20-21	5.464778138701534E-4	0.0	0.0	2.2355910567415366E-4	1.241995031523076E-4
22-23	5.588977641853842E-4	0.0	0.0	5.09217962924461E-4	1.6145935409799986E-4
24-25	5.713177145006149E-4	0.0	0.0	0.0010929556277403068	1.9871920504369216E-4
26-27	5.713177145006149E-4	0.0	0.0	0.002061711752328306	1.9871920504369216E-4
28-29	5.713177145006149E-4	0.0	0.0	0.005812536747527995	1.9871920504369216E-4
30-31	5.961576151310764E-4	0.0	0.0	0.01405938375684122	1.9871920504369216E-4
32-33	6.20997515761538E-4	0.0	0.0	0.0352105591436792	1.9871920504369216E-4
34-35	6.582573667072302E-4	0.0	0.0	0.06525441895622242	1.9871920504369216E-4
36-37	6.70677317022461E-4	0.0	0.0	0.10858762560606253	1.9871920504369216E-4
38-39	6.70677317022461E-4	0.0	0.0	0.1706004375300097	2.111391553589229E-4
40-41	6.70677317022461E-4	0.0	0.0	0.2387611248599961	2.2355910567415366E-4
42-43	6.70677317022461E-4	0.0	0.0	0.3032206669960438	2.2355910567415366E-4
44-45	7.079371679681532E-4	0.0	0.0	0.3812055350253777	2.2355910567415366E-4
46-47	7.20357118283384E-4	0.0	0.0	0.47955912157169	2.2355910567415366E-4
48-49	7.327770685986147E-4	0.0	0.0	0.573528465656726	3.974384100873843E-4
50-51	8.445566214356916E-4	0.0	0.0	0.669174503034318	3.974384100873843E-4
52-53	8.693965220661531E-4	0.0	0.0	0.7620508914916138	4.0985836040261506E-4
54-55	8.693965220661531E-4	0.0	0.0	0.8663287943382911	4.222783107178458E-4
56-57	8.693965220661531E-4	0.0	0.0	0.995980655678985	4.346982610330766E-4
58-59	8.693965220661531E-4	0.0	0.0	1.1241669628824817	4.4711821134830733E-4
60-61	9.190763233270762E-4	0.0	0.0	1.25594263572708	4.7195811197876884E-4
62-63	9.687561245879992E-4	0.0	0.0	1.388128166932081	4.7195811197876884E-4
64-65	9.687561245879992E-4	0.0	0.0	1.5235553051693573	4.7195811197876884E-4
66-67	9.935960252184608E-4	0.0	0.0	1.6780967469417736	4.8437806229399965E-4
68-69	9.935960252184608E-4	0.0	0.0	1.8501751585592956	4.967980126092304E-4
70-71	0.0010184359258489223	0.0	0.0	2.034313341932907	5.216379132396919E-4
72-73	0.0010929556277403068	0.0	0.0	2.200144518541868	6.458374163919995E-4
74-75	0.0010929556277403068	0.0	0.0	2.376532652918775	6.458374163919995E-4
76-77	0.0011302154786859992	0.0	0.0	2.558547024788482	6.70677317022461E-4
78-79	0.0011674753296316914	0.0	0.0	2.788427885173088	6.955172176529225E-4
80-81	0.0011674753296316914	0.0	0.0	3.0255868364424194	7.327770685986147E-4
82-83	0.0011674753296316914	0.0	0.0	3.251716871831826	8.197167208052301E-4
84-85	0.0011674753296316914	0.0	0.0	3.4777599675690256	8.197167208052301E-4
86-87	0.0011923152302621529	0.0	0.0	3.707007410487555	8.197167208052301E-4
88	0.0012171551308926144	0.0	0.0	3.897107170012477	8.445566214356916E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2080	0.0	27.580282	1
TAGGCAT	2785	0.0	24.129217	5
GCATCGT	2200	0.0	23.282856	8
TAGTACT	2995	0.0	23.064972	4
CTAACGC	825	0.0	22.214834	3
CTAGTAC	3340	0.0	22.089487	3
CTATTGA	2725	0.0	22.073738	9
GGGCACC	2920	0.0	21.892008	94
ACGTTAA	460	0.0	21.453289	8
CTGTGCG	3155	0.0	21.448433	9
TTTAGGG	2855	0.0	21.397825	2
TCTAACG	860	0.0	21.310743	2
GCTATTG	2855	0.0	21.068628	8
CGTTAAC	515	0.0	20.987127	9
GGGCTAT	2935	0.0	20.974693	6
CTAGGCA	3065	0.0	20.851671	4
CATGGGG	8540	0.0	20.800144	4
GACGTTA	500	0.0	20.676886	7
CTCGTCG	1775	0.0	20.385662	9
TAACGCC	935	0.0	20.103922	4
>>END_MODULE