FastQCFastQC Report
Mon 16 Jan 2017
ERR1146552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025781
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT918252.280923875392129No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC239420.5947169008945097No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT222530.5527623087296601No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA172590.42871184498113535No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG152740.3794046422296692No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129240.32103087574808464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116810.29015487926442096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107900.26802252780267977No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60580.1504801180193359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58480.14526373888693894No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA43600.10830196674881222No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG42630.10589249638765744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19100.025.1314351
TAGCGCG950.001301718824.7480284
GAGTACT119300.023.1832112-13
TAGGCAT27500.021.0313225
AGTACTT125300.020.72281512-13
GCTATTG26850.020.6540538
CTATTGA25350.020.5784479
GTACTTT133950.020.54242714-15
GGGCTAT28200.019.8324436
CTGTGCG29750.019.4305789
CTAGGCA29800.019.2503094
TACCTGG34300.019.2078632
TAGTACT29200.019.162774
GGGCACC26000.019.16091794
CATGGGG82950.019.1032684
GGCTATT29050.018.6045557
TGCGCGA16450.018.57082694
ACTTTTT149200.018.42722716-17
CTAGTAC32000.018.2207343
ATTTAGG30150.018.1059441
Produced by FastQC (version 0.11.5)