##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4025781
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.519847453202246	34.0	31.0	34.0	31.0	34.0
2	32.527661092344566	34.0	31.0	34.0	31.0	34.0
3	32.68213919237037	34.0	31.0	34.0	31.0	34.0
4	36.11716037211165	37.0	35.0	37.0	35.0	37.0
5	36.15265013173841	37.0	35.0	37.0	35.0	37.0
6	36.26956632762686	37.0	37.0	37.0	35.0	37.0
7	36.2633821859659	37.0	37.0	37.0	35.0	37.0
8	36.27606121644471	37.0	37.0	37.0	35.0	37.0
9	38.07710652914304	39.0	38.0	39.0	37.0	39.0
10-11	38.05044188941227	39.0	38.0	39.0	36.0	39.0
12-13	38.03935422716735	39.0	38.0	39.0	35.0	39.0
14-15	39.42401586176695	41.0	39.0	41.0	36.0	41.0
16-17	39.45274904422273	41.0	39.0	41.0	36.5	41.0
18-19	39.51752864847839	41.0	39.0	41.0	37.0	41.0
20-21	39.54499909458562	41.0	39.0	41.0	37.0	41.0
22-23	39.49858636125512	41.0	39.0	41.0	36.5	41.0
24-25	39.46733130788783	41.0	39.0	41.0	36.0	41.0
26-27	39.358784792317316	41.0	39.0	41.0	36.0	41.0
28-29	39.300965452417806	41.0	39.0	41.0	36.0	41.0
30-31	39.256448748702425	41.0	39.0	41.0	36.0	41.0
32-33	39.128625973444656	41.0	39.0	41.0	35.0	41.0
34-35	39.032480033066875	40.0	39.0	41.0	35.0	41.0
36-37	38.94167069694055	40.0	38.5	41.0	35.0	41.0
38-39	38.841305326842175	40.0	38.0	41.0	35.0	41.0
40-41	38.81848130834737	40.0	38.0	41.0	35.0	41.0
42-43	38.73354698131865	40.0	38.0	41.0	35.0	41.0
44-45	38.59088012984313	40.0	38.0	41.0	35.0	41.0
46-47	38.363833377920955	40.0	38.0	41.0	34.5	41.0
48-49	38.1264016845427	40.0	38.0	41.0	34.0	41.0
50-51	37.92712892231346	40.0	37.0	41.0	34.0	41.0
52-53	37.85347079237544	40.0	37.0	41.0	33.5	41.0
54-55	37.71734043655132	40.0	37.0	41.0	33.0	41.0
56-57	37.46037961329739	40.0	36.0	41.0	33.0	41.0
58-59	37.230097961116115	39.0	36.0	41.0	33.0	41.0
60-61	36.927323046136884	39.0	35.0	41.0	32.0	41.0
62-63	36.70333818456592	39.0	35.0	41.0	32.0	41.0
64-65	36.41027480133668	37.5	35.0	40.0	32.0	41.0
66-67	36.09332636325722	37.0	35.0	40.0	31.5	41.0
68-69	35.709203133503784	36.5	35.0	39.0	31.0	41.0
70-71	35.33738024994405	36.0	35.0	39.0	31.0	41.0
72-73	34.990215314742656	35.5	35.0	38.5	30.5	40.5
74-75	34.71293247198494	35.0	35.0	37.0	31.0	39.5
76-77	34.42422724932131	35.0	34.0	37.0	30.5	39.0
78-79	34.14023949638592	35.0	34.0	36.0	30.0	39.0
80-81	33.88715456702687	35.0	34.0	36.0	30.0	38.0
82-83	33.63006730867874	35.0	34.0	36.0	29.5	37.0
84-85	33.46695336879974	35.0	34.0	35.0	29.0	37.0
86-87	33.39367156335628	35.0	34.0	35.0	30.0	36.5
88-89	33.24985114689547	35.0	34.0	35.0	29.0	36.0
90-91	33.141351578737144	35.0	34.0	35.0	29.0	36.0
92-93	33.050485359238365	35.0	34.0	35.0	29.0	36.0
94-95	32.95804379324161	35.0	34.0	35.0	29.0	35.5
96-97	32.90815608201241	35.0	34.0	35.0	29.0	35.0
98-99	32.932200857423695	35.0	34.0	35.0	29.0	35.0
100	32.29335897804675	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2598282914217833
1101	2	0.24700042076432993
1101	3	0.2751970790399554
1101	4	0.18915423286647837
1101	5	0.18431812321193775
1101	6	0.17885287594027943
1101	7	0.1734735391331057
1101	8	0.16415875871390995
1101	9	0.23243177587067976
1101	10-11	0.23702403276490713
1101	12-13	0.21578085361009158
1101	14-15	0.3372729011435922
1101	16-17	0.3260080479362344
1101	18-19	0.3104301766557356
1101	20-21	0.31978163805700177
1101	22-23	0.2979584900515775
1101	24-25	0.282758314403452
1101	26-27	0.3243875660195741
1101	28-29	0.3463024338421974
1101	30-31	0.36347619796097064
1101	32-33	0.33884457793684675
1101	34-35	0.34868194266068997
1101	36-37	0.38549916156723185
1101	38-39	0.40640378351849193
1101	40-41	0.3375269314977487
1101	42-43	0.3185016811885646
1101	44-45	0.36577437261121304
1101	46-47	0.37046793941325973
1101	48-49	0.4418296053391657
1101	50-51	0.41373032477884664
1101	52-53	0.3806805613856383
1101	54-55	0.3640654184286376
1101	56-57	0.3471673702436959
1101	58-59	0.3633162602938498
1101	60-61	0.3720790139280936
1101	62-63	0.41051495656484605
1101	64-65	0.38311815880780387
1101	66-67	0.3476435352297287
1101	68-69	0.3689117182422734
1101	70-71	0.3692329270690351
1101	72-73	0.2892350246547295
1101	74-75	0.3440993199354452
1101	76-77	0.35788227852286525
1101	78-79	0.30279755065650704
1101	80-81	0.2814282385283775
1101	82-83	0.3472710103339196
1101	84-85	0.36685738791042155
1101	86-87	0.35543596183408255
1101	88-89	0.3329625302578805
1101	90-91	0.34731946886958553
1101	92-93	0.306347915599531
1101	94-95	0.3220032184422408
1101	96-97	0.30330534010895605
1101	98-99	0.3308695315437902
1101	100	0.29524562050765724
1102	1	0.22345828335480178
1102	2	0.19971586471635305
1102	3	0.24733815842724738
1102	4	0.13865077433298012
1102	5	0.18040444639414943
1102	6	0.1583488638991497
1102	7	0.1423261756188623
1102	8	0.17111083673492544
1102	9	0.17761951609909943
1102	10-11	0.18767271480545844
1102	12-13	0.21784665225528244
1102	14-15	0.29595865100452556
1102	16-17	0.23299824166316085
1102	18-19	0.2710483868788245
1102	20-21	0.2529969786801871
1102	22-23	0.29556838558238496
1102	24-25	0.2666890136184463
1102	26-27	0.2617664213910018
1102	28-29	0.3178736115515619
1102	30-31	0.3036099208095493
1102	32-33	0.3070816687422635
1102	34-35	0.2712378156248363
1102	36-37	0.2991835372917677
1102	38-39	0.31437421183633063
1102	40-41	0.3578579631433456
1102	42-43	0.3441369201071325
1102	44-45	0.29570416013171297
1102	46-47	0.3438570599429909
1102	48-49	0.3521387700991241
1102	50-51	0.2865891042359934
1102	52-53	0.3405880848583962
1102	54-55	0.34922298545215114
1102	56-57	0.34316159034959526
1102	58-59	0.31182912164004506
1102	60-61	0.30573938357486696
1102	62-63	0.33328367432923045
1102	64-65	0.36950079677773573
1102	66-67	0.32099379166973563
1102	68-69	0.30800424285872907
1102	70-71	0.29259686930003426
1102	72-73	0.267473709295345
1102	74-75	0.22710688772585996
1102	76-77	0.21974342828195148
1102	78-79	0.24279858357435558
1102	80-81	0.23997153612527455
1102	82-83	0.24211582406008603
1102	84-85	0.26752955512395715
1102	86-87	0.24660917496955648
1102	88-89	0.2541905591727627
1102	90-91	0.2520453755235934
1102	92-93	0.22168382989372049
1102	94-95	0.1931403261344471
1102	96-97	0.15352191571361118
1102	98-99	0.22101221647368874
1102	100	0.2070968634434891
1103	1	0.14525310160733085
1103	2	0.22236382328743076
1103	3	0.19601096425876108
1103	4	0.12786851805068977
1103	5	0.15518066617903514
1103	6	0.13343112836003712
1103	7	0.14340788149927164
1103	8	0.11998527967705996
1103	9	0.15300369243652057
1103	10-11	0.19759393494707211
1103	12-13	0.1644782901837658
1103	14-15	0.22295209594954457
1103	16-17	0.23090193351467292
1103	18-19	0.23617571625511147
1103	20-21	0.23425440684343357
1103	22-23	0.2661071808234894
1103	24-25	0.2522808783549664
1103	26-27	0.2687832623312971
1103	28-29	0.26249083601564394
1103	30-31	0.2552458350978952
1103	32-33	0.2760242245698521
1103	34-35	0.27667059803425786
1103	36-37	0.24993253106385538
1103	38-39	0.30309221309270384
1103	40-41	0.27340518494503385
1103	42-43	0.30572689941703857
1103	44-45	0.3050287345403788
1103	46-47	0.267932313435864
1103	48-49	0.2409873460669445
1103	50-51	0.21441258932572538
1103	52-53	0.24833888923168246
1103	54-55	0.22122670584172965
1103	56-57	0.3007187186891116
1103	58-59	0.22569614294440754
1103	60-61	0.21979422973901563
1103	62-63	0.1790020288311993
1103	64-65	0.14831717606257655
1103	66-67	0.1322012042588554
1103	68-69	0.20052622500155337
1103	70-71	0.1783617243214337
1103	72-73	0.2083096397668669
1103	74-75	0.18635868136082934
1103	76-77	0.18937028869425632
1103	78-79	0.18454198980157344
1103	80-81	0.214139581841863
1103	82-83	0.17136904024812338
1103	84-85	0.19769666378265782
1103	86-87	0.16453384404108107
1103	88-89	0.2205418497372733
1103	90-91	0.2521752324330322
1103	92-93	0.19386041005872556
1103	94-95	0.20354356120285644
1103	96-97	0.18934306381400745
1103	98-99	0.20085176814759365
1103	100	0.17996307732934724
1104	1	0.08975428417038955
1104	2	0.08843331509577013
1104	3	0.1052971955906159
1104	4	0.07366300716436314
1104	5	-0.006751279527627219
1104	6	0.02265874359306963
1104	7	0.027590653682977972
1104	8	0.05074791928576161
1104	9	0.08329671379576808
1104	10-11	0.09449542552218304
1104	12-13	0.07518429605585908
1104	14-15	0.1196059760439141
1104	16-17	0.15157184628267828
1104	18-19	0.11608399470724606
1104	20-21	0.15631193956450318
1104	22-23	0.18059580260194963
1104	24-25	0.17225998828911315
1104	26-27	0.1084566940274243
1104	28-29	0.14561623635201215
1104	30-31	0.16196623453246417
1104	32-33	0.11528843286545509
1104	34-35	0.13853108126292568
1104	36-37	0.1729184644903512
1104	38-39	0.1943857353511973
1104	40-41	0.1944463370375047
1104	42-43	0.1355652609617337
1104	44-45	0.1104256283199021
1104	46-47	0.1327796957366374
1104	48-49	0.10409535671874437
1104	50-51	0.1087765386643369
1104	52-53	0.1570074467359106
1104	54-55	0.15418576918834503
1104	56-57	0.168650539002968
1104	58-59	0.07990244005574709
1104	60-61	0.10832581743778036
1104	62-63	0.17402006698340955
1104	64-65	0.1659486248212687
1104	66-67	0.11182913973410535
1104	68-69	0.11615998015469131
1104	70-71	0.08732503220794996
1104	72-73	0.11257145646005284
1104	74-75	0.0336502470614235
1104	76-77	0.05098270785083514
1104	78-79	0.08543076678219563
1104	80-81	0.09886135495264625
1104	82-83	0.09425511637782336
1104	84-85	0.07287942749932341
1104	86-87	0.11003916026616167
1104	88-89	0.08298581944516314
1104	90-91	0.07189407164773343
1104	92-93	0.1362405154478239
1104	94-95	0.05454704975716851
1104	96-97	-0.016437054181565713
1104	98-99	-0.005183637782394612
1104	100	0.10919140218086199
1105	1	0.21049989638796518
1105	2	0.1862804164797538
1105	3	0.15757598352009694
1105	4	0.1508794175346324
1105	5	0.09931449060538
1105	6	0.08447430599096606
1105	7	0.12147105047129259
1105	8	0.1190399888292788
1105	9	0.16540584515570345
1105	10-11	0.15322708625365067
1105	12-13	0.13184343711665036
1105	14-15	0.17751414147519284
1105	16-17	0.1476169905402429
1105	18-19	0.17641520310399272
1105	20-21	0.16883083911904606
1105	22-23	0.22015332586973813
1105	24-25	0.20830510276613978
1105	26-27	0.24204334253683868
1105	28-29	0.25449911886499876
1105	30-31	0.2481051957289253
1105	32-33	0.23588294052279224
1105	34-35	0.24314042457160667
1105	36-37	0.20399566211364117
1105	38-39	0.2381592643540884
1105	40-41	0.23607330687767814
1105	42-43	0.22192309328816862
1105	44-45	0.17354304386577013
1105	46-47	0.19348503009035767
1105	48-49	0.16574646083543598
1105	50-51	0.20335391097901123
1105	52-53	0.2110824606218813
1105	54-55	0.17711800489968255
1105	56-57	0.18934050596431007
1105	58-59	0.19301902238012048
1105	60-61	0.23135320514328583
1105	62-63	0.22813206893397364
1105	64-65	0.20890486808342246
1105	66-67	0.11281069366748397
1105	68-69	0.144325464660632
1105	70-71	0.15786356829036663
1105	72-73	0.1681041644273904
1105	74-75	0.08200714377198892
1105	76-77	0.07061224996932225
1105	78-79	0.05743454029345685
1105	80-81	0.08562426915719357
1105	82-83	0.12335474356347476
1105	84-85	0.10343955257252446
1105	86-87	0.1012323416883234
1105	88-89	0.10079269745735786
1105	90-91	0.13927637736867382
1105	92-93	0.0982787855963494
1105	94-95	0.12920550181031132
1105	96-97	0.0930316337347108
1105	98-99	0.07758434050797547
1105	100	0.19523862980899054
1106	1	0.1485298729689859
1106	2	0.12783754829035843
1106	3	0.1299855483517831
1106	4	0.1245284976630785
1106	5	0.09820008414007475
1106	6	0.08823523618023899
1106	7	0.074569637312635
1106	8	0.06649346800307399
1106	9	0.09222890207006174
1106	10-11	0.07335089890710833
1106	12-13	0.06162255840656172
1106	14-15	0.16512947937305
1106	16-17	0.1493028636069056
1106	18-19	0.13913167414758476
1106	20-21	0.12881208000867872
1106	22-23	0.1481388849078229
1106	24-25	0.12224644397850426
1106	26-27	0.13637349867097015
1106	28-29	0.19985208027700452
1106	30-31	0.18925779796256137
1106	32-33	0.18589268891155797
1106	34-35	0.2140617293731495
1106	36-37	0.19990711031118735
1106	38-39	0.22366930498409943
1106	40-41	0.22467549253678243
1106	42-43	0.17423297145104755
1106	44-45	0.1821582087394944
1106	46-47	0.22974941204492438
1106	48-49	0.21116938339457647
1106	50-51	0.25016263478595846
1106	52-53	0.21230136993499116
1106	54-55	0.21482835276475498
1106	56-57	0.2215215815491618
1106	58-59	0.1880564371952005
1106	60-61	0.2137218928736857
1106	62-63	0.17090725335376078
1106	64-65	0.19703975933132511
1106	66-67	0.19220033330956454
1106	68-69	0.19799078068096776
1106	70-71	0.17093299232922732
1106	72-73	0.15819521131236058
1106	74-75	0.14340243910277906
1106	76-77	0.1812990722038066
1106	78-79	0.16259971040358323
1106	80-81	0.1471019583628106
1106	82-83	0.21329303863731042
1106	84-85	0.17614164121065556
1106	86-87	0.18550298425473954
1106	88-89	0.22530320389382297
1106	90-91	0.1808435611718835
1106	92-93	0.14346775283706847
1106	94-95	0.18558777163440965
1106	96-97	0.20507252911234986
1106	98-99	0.19865427892990084
1106	100	0.140653935218225
1107	1	0.1032439684917108
1107	2	0.08990470601310818
1107	3	0.1275120893968591
1107	4	0.06492552030908882
1107	5	0.07152669895020836
1107	6	0.06207701246674446
1107	7	0.08382278125230869
1107	8	0.08845856475537062
1107	9	0.08116240596092439
1107	10-11	0.07192042605969107
1107	12-13	0.04058527680396651
1107	14-15	0.07635893898262935
1107	16-17	0.073119846769373
1107	18-19	0.0626235445760912
1107	20-21	0.11193904931759846
1107	22-23	0.1123653858017164
1107	24-25	0.08051643739018743
1107	26-27	0.11230795545698413
1107	28-29	0.13696901260139782
1107	30-31	0.10351631606397405
1107	32-33	0.06135069232672663
1107	34-35	0.06844731600234155
1107	36-37	0.12057160544482315
1107	38-39	0.13893019726764777
1107	40-41	0.15106380606188452
1107	42-43	0.15768708191687608
1107	44-45	0.16095685669706938
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2216	42-43	-0.6170474159030732
2216	44-45	-0.5468939499058649
2216	46-47	-0.6024853490392559
2216	48-49	-0.5927733052129014
2216	50-51	-0.5418792311106628
2216	52-53	-0.6102916735725188
2216	54-55	-0.6395599758007648
2216	56-57	-0.6473709125169194
2216	58-59	-0.6409048637018202
2216	60-61	-0.6767889508802298
2216	62-63	-0.6845591581443315
2216	64-65	-0.5928238233772305
2216	66-67	-0.5880421389551458
2216	68-69	-0.5978382563757734
2216	70-71	-0.6504782256840613
2216	72-73	-0.6352387178177423
2216	74-75	-0.5612067253090416
2216	76-77	-0.5613498954634579
2216	78-79	-0.6411512054838582
2216	80-81	-0.6927458530343671
2216	82-83	-0.6341927786963097
2216	84-85	-0.6777845263456825
2216	86-87	-0.6576706084542749
2216	88-89	-0.6463903072049533
2216	90-91	-0.6419877817630493
2216	92-93	-0.7273499229846649
2216	94-95	-0.6468292511854159
2216	96-97	-0.7047695617704619
2216	98-99	-0.6794835131700481
2216	100	-0.8232102791996354
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	19.0
8	73.0
9	124.0
10	221.0
11	217.0
12	161.0
13	206.0
14	371.0
15	571.0
16	1161.0
17	2222.0
18	4065.0
19	6449.0
20	9102.0
21	13232.0
22	19359.0
23	25035.0
24	25230.0
25	22344.0
26	20620.0
27	22045.0
28	27204.0
29	33246.0
30	42598.0
31	54085.0
32	70844.0
33	94108.0
34	143102.0
35	234808.0
36	457884.0
37	1073426.0
38	1238006.0
39	382661.0
40	981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34905789271031	19.089838655587435	13.082535305734618	22.478568145967632
2	14.767205974483149	22.623233659172044	37.18752796809896	25.422032398245843
3	21.89961955047539	25.750938207008655	29.99721295138016	22.352229291135796
4	12.15307042285708	16.979264396150707	39.99852450990255	30.869140671089664
5	12.214187184920359	39.626497913488485	34.82129655698886	13.338018344602293
6	29.079880604370818	37.83055876280056	17.382119669873468	15.707440962955161
7	25.435102125576194	33.162575491600506	23.244023082240727	18.15829930058257
8	25.567958117940343	30.90955518941542	20.579559593529805	22.94292709911443
9	24.790667947411944	17.28357801877449	22.508253677981987	35.41750035583158
10-11	25.456948602022813	24.65546436828034	27.74767181821366	22.13991521148319
12-13	28.60711996007731	23.185911007081607	26.578867553898238	21.628101478942842
14-15	23.90629242191387	24.533686523775895	27.81036912359074	23.749651930719494
16-17	21.381938063694967	27.268274155996064	28.3553799871379	22.994407793171064
18-19	21.025746308604468	29.119591949984365	28.489192531834195	21.365469209576972
20-21	23.25030844946608	25.716264744654517	27.25595356528336	23.77747324059605
22-23	22.236633595855487	25.961984030078327	27.62040566050273	24.180976713563453
24-25	24.132919798667636	26.13345087574312	27.198896313535187	22.53473301205406
26-27	23.480164469950054	24.246959782462085	29.981536501861378	22.291339245726483
28-29	21.44843980545385	28.379754979159316	28.26487041020622	21.90693480518061
30-31	22.92680963868829	25.428911096891632	27.800416615330807	23.843862649089267
32-33	23.114413947593686	26.296570184847447	28.08357705918328	22.50543880837559
34-35	24.16847990586516	25.533281110719603	28.27212411610411	22.02611486731113
36-37	23.56980624305174	24.549253621601697	29.81730437351945	22.063635761827115
38-39	22.920645479644104	26.218664072698992	29.071610374264377	21.789080073392526
40-41	22.449897415751312	26.647983445427283	28.335882155606875	22.566236983214534
42-43	22.315858293786885	26.643621693986823	26.44348514805248	24.597034864173807
44-45	23.511543730973298	25.152390612795383	27.1481024746529	24.18796318157842
46-47	24.70316500211699	26.485584659903644	27.326903331504692	21.48434700647467
48-49	24.200624405157644	26.04162229089401	27.426481738875335	22.331271565073006
50-51	21.21220213081348	26.77520490334757	29.25897217144825	22.753620794390695
52-53	22.737034368553573	28.03300619179704	25.74289951672113	23.487059922928253
54-55	22.031938232976657	26.01096502776655	26.890313455422003	25.06678328383479
56-57	24.293589326083982	24.83290645518748	26.752156560571862	24.12134765815668
58-59	25.638404317103618	25.260603100806772	26.3965156538229	22.704476928266704
60-61	23.483320327292674	26.351807458764558	28.61887026421659	21.54600194972618
62-63	22.239178406032885	26.47097654577654	27.808011728540038	23.481833319650534
64-65	21.412705017295668	28.83068878358992	26.467622637759824	23.28898356135459
66-67	23.51799142683397	25.747941168287632	25.726217612152674	25.00784979272573
68-69	22.527667842802536	26.06514239325598	26.713230187111925	24.69395957682956
70-71	24.273167229818895	25.934703321966783	26.105021421727017	23.6871080264873
72-73	23.868607581132512	24.429014153187897	28.825359555609598	22.87701871006999
74-75	22.44805338204795	28.536837652209364	26.325850681993817	22.689258283748874
76-77	23.06100391317924	25.735382339198342	26.26582144843524	24.937792299187183
78-79	23.418401793658703	26.1727290747684	26.983127068875323	23.425742062697573
80-81	24.215179747221562	25.759502168338372	26.898573778959772	23.126744305480294
82-83	23.435961669468405	24.892005199002178	28.74439626183546	22.927636869693956
84-85	22.855883118229954	26.908504009473944	27.177892966017126	23.057719906278972
86-87	22.553828078037053	27.449810968309247	26.539577423505506	23.456783530148194
88-89	22.469611441275283	26.832585030211575	25.41139283740398	25.286410691109168
90-91	23.430065300380885	25.043739965455146	26.30704778862136	25.219146945542608
92-93	24.648372576650342	26.748263256247668	26.366697542663147	22.236666624438836
94-95	23.035629608267318	26.244708790666955	27.197604638702405	23.522056962363326
96-97	21.112903726611645	26.505082872241992	28.92014581043402	23.461867590712348
98-99	22.803002612052403	27.682427287359175	25.70409349883246	23.810476601755965
100	22.59613258872526	24.361943711246276	25.787121976520105	27.254801723508358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	460.0
1	320.5
2	442.0
3	727.5
4	476.5
5	285.5
6	578.5
7	1373.0
8	2579.5
9	3192.0
10	2414.0
11	1539.0
12	1472.5
13	1668.5
14	1977.5
15	2373.5
16	2830.0
17	3305.5
18	3922.0
19	4543.0
20	5026.0
21	5654.5
22	6597.5
23	8013.5
24	9723.5
25	11629.0
26	14950.0
27	18619.0
28	21972.0
29	27074.5
30	33474.0
31	39845.0
32	47567.0
33	55137.0
34	61896.0
35	68892.5
36	76254.5
37	87097.0
38	92415.5
39	90861.5
40	89349.5
41	87653.0
42	89098.0
43	96179.0
44	106900.5
45	120987.5
46	134932.0
47	205225.0
48	348817.0
49	344841.0
50	234654.5
51	182434.0
52	145464.5
53	131835.0
54	127427.0
55	117697.0
56	107498.5
57	95222.5
58	83163.0
59	77163.5
60	66600.5
61	52561.0
62	41775.0
63	32828.0
64	23479.0
65	15773.5
66	11344.5
67	8126.5
68	5988.0
69	4562.0
70	3591.5
71	2928.5
72	2527.0
73	2341.0
74	1942.5
75	1484.5
76	1002.5
77	597.5
78	356.5
79	178.0
80	111.0
81	83.0
82	45.5
83	22.0
84	12.0
85	7.0
86	6.0
87	7.0
88	5.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005738017045636611
2	0.08299010800637192
3	4.222783107178458E-4
4	0.0
5	0.03974384100873843
6	0.004297302809069843
7	4.967980126092304E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.967980126092304E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.334174660767687E-4
24-25	0.0
26-27	0.0
28-29	2.483990063046152E-5
30-31	1.241995031523076E-4
32-33	0.002061711752328306
34-35	0.0016518533919256911
36-37	0.001030855876164153
38-39	7.576169692290762E-4
40-41	0.0016270134912952296
42-43	7.327770685986147E-4
44-45	6.085775654463072E-4
46-47	4.346982610330766E-4
48-49	7.327770685986147E-4
50-51	0.001030855876164153
52-53	0.0012792548824687683
54-55	0.0018133127460236908
56-57	0.0010432758264793838
58-59	0.0029559481750249207
60-61	0.0021734913051653826
62-63	0.001130215478685999
64-65	0.005117019529875073
66-67	0.00489346042420092
68-69	0.004197943206547997
70-71	0.0019499321994912292
72-73	0.005502037989647227
74-75	0.00392470429961292
76-77	0.0038253446970910737
78-79	0.0010681157271098453
80-81	6.20997515761538E-5
82-83	2.111391553589229E-4
84-85	8.693965220661531E-5
86-87	2.483990063046152E-5
88-89	1.490394037827691E-4
90-91	1.241995031523076E-5
92-93	0.0
94-95	0.0
96-97	1.490394037827691E-4
98-99	4.843780622939996E-4
100	9.687561245879992E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4025781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.23453692573364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.23724534483974	32.23154684951179
2	15.61755050643753	14.753755321716675
3	6.478969810103212	9.18093416208101
4	3.339209056696082	6.309039739650212
5	1.8946071621632719	4.474544598048025
6	1.1241077196954674	3.1858024556674684
7	0.770741459115071	2.5483931137535256
8	0.5525337779666228	2.087894171046365
9	0.3890912222832593	1.6540689335775702
>10	1.5496015337261624	11.498792906003075
>50	0.03084232391361486	0.9838942425526187
>100	0.012692797562535583	1.0640587047432393
>500	0.0011059003474766555	0.3909715395960905
>1k	8.506925749819631E-4	0.882478617945399
>5k	1.701385149963926E-4	0.478407723579725
>10k+	6.805540599855704E-4	8.27541692052723
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	91825	2.280923875392129	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	23942	0.5947169008945097	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	22253	0.5527623087296601	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	17259	0.42871184498113535	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	15274	0.3794046422296692	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	12924	0.32103087574808464	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11681	0.29015487926442096	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10790	0.26802252780267977	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	6058	0.1504801180193359	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5848	0.14526373888693894	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	4360	0.10830196674881222	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	4263	0.10589249638765744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.4775860882646124E-4	0.0	0.0	0.0	0.0
7	3.4775860882646124E-4	0.0	0.0	0.0	0.0
8	3.974384100873843E-4	0.0	0.0	0.0	0.0
9	3.974384100873843E-4	0.0	0.0	2.483990063046152E-5	0.0
10-11	3.974384100873843E-4	0.0	0.0	2.483990063046152E-5	0.0
12-13	4.222783107178458E-4	0.0	0.0	2.483990063046152E-5	0.0
14-15	4.4711821134830733E-4	0.0	0.0	3.725985094569228E-5	0.0
16-17	6.458374163919995E-4	0.0	0.0	4.967980126092304E-5	0.0
18-19	6.458374163919995E-4	0.0	0.0	7.451970189138455E-5	2.483990063046152E-5
20-21	6.458374163919995E-4	0.0	0.0	2.2355910567415366E-4	2.483990063046152E-5
22-23	6.830972673376917E-4	0.0	0.0	5.216379132396919E-4	4.967980126092304E-5
24-25	7.327770685986147E-4	0.0	0.0	0.0011177955283707683	4.967980126092304E-5
26-27	7.451970189138455E-4	0.0	0.0	0.002074131702643537	4.967980126092304E-5
28-29	7.451970189138455E-4	0.0	0.0	0.005787696846897534	6.20997515761538E-5
30-31	7.451970189138455E-4	0.0	0.0	0.014096643607786912	7.451970189138455E-5
32-33	7.451970189138455E-4	0.0	0.0	0.035272658895255354	7.451970189138455E-5
34-35	7.70036919544307E-4	0.0	0.0	0.06557733766441841	1.241995031523076E-4
36-37	7.948768201747685E-4	0.0	0.0	0.10919620317150883	1.241995031523076E-4
38-39	8.197167208052301E-4	0.0	0.0	0.1715195138533368	1.241995031523076E-4
40-41	8.445566214356916E-4	0.0	0.0	0.2398044006864755	1.241995031523076E-4
42-43	8.445566214356916E-4	0.0	0.0	0.3044626620275668	1.3661945346753835E-4
44-45	8.569765717509224E-4	0.0	0.0	0.38280770861604246	1.490394037827691E-4
46-47	8.693965220661531E-4	0.0	0.0	0.48121097496361576	2.111391553589229E-4
48-49	8.693965220661531E-4	0.0	0.0	0.5756150173096848	2.980788075655382E-4
50-51	9.563361742727684E-4	0.0	0.0	0.6714721938426358	2.980788075655382E-4
52-53	9.687561245879992E-4	0.0	0.0	0.764708760859073	3.2291870819599973E-4
54-55	9.687561245879992E-4	0.0	0.0	0.8690611834076418	3.7259850945692275E-4
56-57	9.687561245879992E-4	0.0	0.0	0.9986509449967598	3.974384100873843E-4
58-59	9.687561245879992E-4	0.0	0.0	1.1274334098153873	3.974384100873843E-4
60-61	0.0011053755780555375	0.0	0.0	1.25943264176566	3.974384100873843E-4
62-63	0.001241995031523076	0.0	0.0	1.3918168921757046	4.222783107178458E-4
64-65	0.001241995031523076	0.0	0.0	1.5282376264381992	4.346982610330766E-4
66-67	0.001241995031523076	0.0	0.0	1.6836484647326817	4.4711821134830733E-4
68-69	0.001241995031523076	0.0	0.0	1.8567701521766833	4.4711821134830733E-4
70-71	0.0013040947830992296	0.0	0.0	2.041864671724567	4.7195811197876884E-4
72-73	0.001440714236566768	0.0	0.0	2.208813643861899	5.713177145006149E-4
74-75	0.001440714236566768	0.0	0.0	2.3865058730219055	5.713177145006149E-4
76-77	0.0014531341868819986	0.0	0.0	2.569700140171559	5.713177145006149E-4
78-79	0.0014655541371972295	0.0	0.0	2.8011210743952537	5.713177145006149E-4
80-81	0.0014655541371972295	0.0	0.0	3.0398946192055654	5.713177145006149E-4
82-83	0.0014655541371972295	0.0	0.0	3.266794691514516	5.961576151310764E-4
84-85	0.0014655541371972295	0.0	0.0	3.4940176825316627	5.961576151310764E-4
86-87	0.0014779740875124603	0.0	0.0	3.724258721475411	5.961576151310764E-4
88	0.0015649137397190757	0.0	0.0	3.9152651373733445	5.961576151310764E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1910	0.0	25.131435	1
TAGCGCG	95	0.0013017188	24.748028	4
GAGTACT	11930	0.0	23.18321	12-13
TAGGCAT	2750	0.0	21.031322	5
AGTACTT	12530	0.0	20.722815	12-13
GCTATTG	2685	0.0	20.654053	8
CTATTGA	2535	0.0	20.578447	9
GTACTTT	13395	0.0	20.542427	14-15
GGGCTAT	2820	0.0	19.832443	6
CTGTGCG	2975	0.0	19.430578	9
CTAGGCA	2980	0.0	19.250309	4
TACCTGG	3430	0.0	19.207863	2
TAGTACT	2920	0.0	19.16277	4
GGGCACC	2600	0.0	19.160917	94
CATGGGG	8295	0.0	19.103268	4
GGCTATT	2905	0.0	18.604555	7
TGCGCGA	1645	0.0	18.570826	94
ACTTTTT	14920	0.0	18.427227	16-17
CTAGTAC	3200	0.0	18.220734	3
ATTTAGG	3015	0.0	18.105944	1
>>END_MODULE