FastQCFastQC Report
Mon 16 Jan 2017
ERR1146551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141082
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.042.1837041
GTATCAA49100.037.5345151
CAACGCA54650.031.1260035
ATCAACG56300.030.2967663
TATCAAC62950.028.3652022
AACGCAG64150.027.4679166
TCAACGC67200.025.8025154
GTACATG105650.025.320311
ACGCAGA67800.025.088237
TACATGG105350.024.7992362
CGCAGAG71400.023.6916548
ACATGGG110400.022.9412843
GTACTAG13600.021.7784961
TAGTACT18850.021.4383834
CATGGGG85950.021.0484334
GTATAAG16750.019.6475981
GTGGTAT14950.019.4974021
GCAGAGT86000.019.068579
CTGTGCG18600.018.9469579
AGAGTAC68950.018.77568210-11
Produced by FastQC (version 0.11.5)