##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146551_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3141082
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16938780967832	31.0	31.0	34.0	28.0	34.0
2	31.318091982316922	31.0	31.0	34.0	28.0	34.0
3	31.344304924226748	33.0	31.0	34.0	28.0	34.0
4	34.88028965814964	37.0	35.0	37.0	32.0	37.0
5	34.879714378675885	37.0	35.0	37.0	32.0	37.0
6	34.98834573564141	37.0	35.0	37.0	32.0	37.0
7	34.832486385264694	37.0	35.0	37.0	32.0	37.0
8	34.84409130356992	37.0	35.0	37.0	32.0	37.0
9	36.39556369429388	39.0	37.0	39.0	32.0	39.0
10-11	36.38914902571789	39.0	37.0	39.0	32.0	39.0
12-13	36.39834585661883	39.0	37.0	39.0	32.0	39.0
14-15	37.75606128716156	40.0	38.0	41.0	32.0	41.0
16-17	37.76162529344984	40.0	38.0	41.0	32.0	41.0
18-19	37.77582358563068	40.0	38.0	41.0	32.0	41.0
20-21	37.86002641764844	40.0	38.0	41.0	32.0	41.0
22-23	37.88214013515088	40.0	38.0	41.0	32.0	41.0
24-25	37.93029663026944	40.0	38.0	41.0	32.0	41.0
26-27	37.95326037333632	40.0	38.0	41.0	32.0	41.0
28-29	37.90945046961525	40.0	38.0	41.0	32.0	41.0
30-31	37.90516595873652	40.0	38.0	41.0	32.0	41.0
32-33	37.91719206948434	40.0	38.0	41.0	32.0	41.0
34-35	37.868701613011055	40.0	38.0	41.0	31.5	41.0
36-37	37.78136132708411	40.0	38.0	41.0	31.0	41.0
38-39	37.739196079567485	40.0	38.0	41.0	31.0	41.0
40-41	37.668578375222296	40.0	38.0	41.0	31.0	41.0
42-43	37.5518203599906	40.0	37.0	41.0	30.5	41.0
44-45	37.422721692716074	40.0	37.0	41.0	30.0	41.0
46-47	37.31370288964121	40.0	37.0	41.0	30.0	41.0
48-49	37.19646112390571	40.0	36.5	41.0	30.0	41.0
50-51	37.07404247962963	40.0	36.0	41.0	30.0	41.0
52-53	36.94780031212174	40.0	36.0	41.0	30.0	41.0
54-55	36.763835200736565	39.0	35.0	41.0	30.0	41.0
56-57	36.62686918074727	39.0	35.0	41.0	30.0	41.0
58-59	36.410826110238446	39.0	35.0	41.0	29.5	41.0
60-61	36.23380112330719	38.5	35.0	40.0	29.0	41.0
62-63	35.995334410244624	38.0	35.0	40.0	29.0	41.0
64-65	35.75013371188654	37.0	35.0	40.0	29.0	41.0
66-67	35.492203005206484	37.0	34.5	40.0	29.0	41.0
68-69	35.20187502268327	36.5	34.0	39.5	29.0	41.0
70-71	34.94577696475291	36.0	34.0	39.0	28.5	41.0
72-73	34.62959642569025	35.5	34.0	39.0	28.0	40.5
74-75	34.31411484959641	35.0	34.0	37.5	28.0	40.0
76-77	34.01818258803813	35.0	34.0	37.0	28.0	39.0
78-79	33.74248666542293	35.0	34.0	37.0	28.0	39.0
80-81	33.469937906746786	35.0	33.0	36.0	27.0	39.0
82-83	33.224457050150235	35.0	33.0	36.0	27.0	37.0
84-85	33.00706110187508	35.0	33.0	35.5	27.0	37.0
86-87	32.81230305353378	35.0	33.0	35.0	26.5	37.0
88-89	32.67479947992443	35.0	33.0	35.0	26.5	36.0
90-91	32.520995790622464	35.0	33.0	35.0	26.0	36.0
92-93	32.43318560292282	35.0	33.0	35.0	26.0	36.0
94-95	32.339146033118524	35.0	33.0	35.0	26.0	35.5
96-97	32.270605638439235	35.0	33.0	35.0	25.0	35.0
98-99	32.19896806259754	35.0	33.0	35.0	25.0	35.0
100	31.254700131992735	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3855479149075549
1101	2	0.42029697544085565
1101	3	0.4680155651887574
1101	4	0.4094338344566637
1101	5	0.436450031694136
1101	6	0.47118648573904665
1101	7	0.4427338952081499
1101	8	0.5085643713196433
1101	9	0.6766529438245072
1101	10-11	0.6830898587171887
1101	12-13	0.6752583481434655
1101	14-15	0.8826978043268454
1101	16-17	0.7709495250125187
1101	18-19	0.6700459630716722
1101	20-21	0.69288641250008
1101	22-23	0.7796828922128398
1101	24-25	0.6549811529851794
1101	26-27	0.6394453897773644
1101	28-29	0.6849684297179266
1101	30-31	0.7128717156395012
1101	32-33	0.6902256656228971
1101	34-35	0.6375166785955884
1101	36-37	0.6163641450065711
1101	38-39	0.6426809883110636
1101	40-41	0.6576411404153717
1101	42-43	0.598551100002993
1101	44-45	0.5740180058703999
1101	46-47	0.5446071757122439
1101	48-49	0.586729694854526
1101	50-51	0.5859057767225835
1101	52-53	0.5679491960701668
1101	54-55	0.6339855087755168
1101	56-57	0.5394211056682892
1101	58-59	0.5647342057302325
1101	60-61	0.584558526906946
1101	62-63	0.6137564211486506
1101	64-65	0.5995072830672115
1101	66-67	0.5462048043659493
1101	68-69	0.4734596206164383
1101	70-71	0.4577712993177414
1101	72-73	0.44143178899162194
1101	74-75	0.4318150015814268
1101	76-77	0.43089457840475376
1101	78-79	0.4748854531724902
1101	80-81	0.41966217975014786
1101	82-83	0.40250077218001934
1101	84-85	0.4186602075736161
1101	86-87	0.44842568593732324
1101	88-89	0.37661685929152355
1101	90-91	0.46156384543787965
1101	92-93	0.36879533036042034
1101	94-95	0.3737575563899682
1101	96-97	0.3589032111565089
1101	98-99	0.36488662788708837
1101	100	0.38854812347480916
1102	1	0.22319301807207736
1102	2	0.2777287951329974
1102	3	0.3254396298991864
1102	4	0.2857167619598684
1102	5	0.25595521852417136
1102	6	0.19484164962030803
1102	7	0.22897868132515242
1102	8	0.34398630018880283
1102	9	0.42398233691128695
1102	10-11	0.44075255614559694
1102	12-13	0.3809403277970205
1102	14-15	0.5038457408634187
1102	16-17	0.44269615210252766
1102	18-19	0.4154836775043691
1102	20-21	0.3430329398968226
1102	22-23	0.34678829995247185
1102	24-25	0.3898373440077947
1102	26-27	0.41206598541006656
1102	28-29	0.513718091630551
1102	30-31	0.4793865159027888
1102	32-33	0.4500910164719869
1102	34-35	0.5005298481055291
1102	36-37	0.4515406585314352
1102	38-39	0.3934385307167858
1102	40-41	0.3955520783875741
1102	42-43	0.4012360894246001
1102	44-45	0.38018665238389815
1102	46-47	0.3040127456264443
1102	48-49	0.3729477262846359
1102	50-51	0.3583833227695834
1102	52-53	0.35764581402719386
1102	54-55	0.3800742240959778
1102	56-57	0.27669673167726216
1102	58-59	0.3664025767032655
1102	60-61	0.36515258594668865
1102	62-63	0.3554584415838704
1102	64-65	0.32669448319548167
1102	66-67	0.33155436732501187
1102	68-69	0.3479215292796951
1102	70-71	0.29704312920351583
1102	72-73	0.3600235403874308
1102	74-75	0.286826431827933
1102	76-77	0.2675800920631346
1102	78-79	0.25581956816025553
1102	80-81	0.23282173348793833
1102	82-83	0.24343169092225025
1102	84-85	0.29907242422299163
1102	86-87	0.23596161685547656
1102	88-89	0.22498492480179522
1102	90-91	0.23101492039168647
1102	92-93	0.2576038657080417
1102	94-95	0.23752200466656603
1102	96-97	0.16755049895903085
1102	98-99	0.1990728600743381
1102	100	0.21299943763997575
1103	1	0.22665716928900181
1103	2	0.1991446571143598
1103	3	0.27868356379019943
1103	4	0.19994862176987738
1103	5	0.2385861578837165
1103	6	0.22088277857723426
1103	7	0.12948913185860533
1103	8	0.2477900924565759
1103	9	0.3323029767219978
1103	10-11	0.33735894101320696
1103	12-13	0.43787922293405757
1103	14-15	0.5779235211450313
1103	16-17	0.46633734494723456
1103	18-19	0.4512949602367087
1103	20-21	0.42768607374205914
1103	22-23	0.4001576884922571
1103	24-25	0.41496848944772324
1103	26-27	0.4275928417713004
1103	28-29	0.5425596271917925
1103	30-31	0.48539674595023996
1103	32-33	0.5213235155027576
1103	34-35	0.4935957434084628
1103	36-37	0.4640890959962434
1103	38-39	0.42476689342557705
1103	40-41	0.4284303027738403
1103	42-43	0.4367877851889048
1103	44-45	0.39855570307761923
1103	46-47	0.3718671555808726
1103	48-49	0.3279545478025625
1103	50-51	0.32605819983876927
1103	52-53	0.35495427846024086
1103	54-55	0.35993859862287536
1103	56-57	0.3272050328008902
1103	58-59	0.41517154224376895
1103	60-61	0.38646995808281304
1103	62-63	0.31783473761796444
1103	64-65	0.28291254859631465
1103	66-67	0.2831789267102849
1103	68-69	0.29023757209731116
1103	70-71	0.27444377130539976
1103	72-73	0.26432401210284695
1103	74-75	0.20875466509194496
1103	76-77	0.18575594739962042
1103	78-79	0.2419650784032754
1103	80-81	0.28151841369016495
1103	82-83	0.26757090659575056
1103	84-85	0.2568833966628645
1103	86-87	0.18496109516627257
1103	88-89	0.16765430544135285
1103	90-91	0.19203668808233232
1103	92-93	0.1587096913289372
1103	94-95	0.14937502230647937
1103	96-97	0.13414452586346926
1103	98-99	0.14218492767282243
1103	100	0.021507259033622717
1104	1	0.10798266303181592
1104	2	0.17229138479024186
1104	3	0.1868747504775179
1104	4	0.2311042226715898
1104	5	0.18279392958574192
1104	6	0.1951693160792587
1104	7	0.15825343647736645
1104	8	0.1735953531449681
1104	9	0.33299199429504966
1104	10-11	0.3164155687461303
1104	12-13	0.2922265844320222
1104	14-15	0.4045942539305756
1104	16-17	0.38896592301881583
1104	18-19	0.32793090711371775
1104	20-21	0.32796954716419435
1104	22-23	0.32839850754282907
1104	24-25	0.3403263081819574
1104	26-27	0.36551686908555325
1104	28-29	0.2953171819660412
1104	30-31	0.2547516543369923
1104	32-33	0.30455664221253187
1104	34-35	0.28485144688379904
1104	36-37	0.2975856951159841
1104	38-39	0.25357590683010045
1104	40-41	0.3315403389565219
1104	42-43	0.3670222500026199
1104	44-45	0.1962385056010305
1104	46-47	0.17513596869416403
1104	48-49	0.16117743022434183
1104	50-51	0.19352369761610788
1104	52-53	0.19155573305651785
1104	54-55	0.1887248136518096
1104	56-57	0.19489007049119778
1104	58-59	0.2444443405240122
1104	60-61	0.19161638221616784
1104	62-63	0.18569162112850535
1104	64-65	0.16401101192042233
1104	66-67	0.21327799041885953
1104	68-69	0.14601243331730274
1104	70-71	0.18146878158645308
1104	72-73	0.1888044066166259
1104	74-75	0.15702321699087918
1104	76-77	0.15615009127321855
1104	78-79	0.17585676220413404
1104	80-81	0.20520876413742428
1104	82-83	0.19136124848476044
1104	84-85	0.15109103489769637
1104	86-87	0.09622259928923427
1104	88-89	0.08254777873764851
1104	90-91	0.15448773626556545
1104	92-93	0.07148444103573581
1104	94-95	0.05232536090729667
1104	96-97	0.12302126106635569
1104	98-99	0.1005761762344406
1104	100	0.055858990628809835
1105	1	0.15856502348725243
1105	2	0.2432513013490727
1105	3	0.18863618546662053
1105	4	0.1576654703778999
1105	5	0.17324595374224572
1105	6	0.2034122873541122
1105	7	0.10732685417462307
1105	8	0.1260159612461038
1105	9	0.17556785246504347
1105	10-11	0.2307946411182087
1105	12-13	0.22266042988201917
1105	14-15	0.32249130213137533
1105	16-17	0.2742825161177507
1105	18-19	0.25115974882606196
1105	20-21	0.21124023283504556
1105	22-23	0.2758224694108762
1105	24-25	0.2950066623572809
1105	26-27	0.3374833337614973
1105	28-29	0.3345677000703944
1105	30-31	0.32824605209074775
1105	32-33	0.3038274139388477
1105	34-35	0.26763314074587896
1105	36-37	0.30132949006090826
1105	38-39	0.2762767819703029
1105	40-41	0.2533866089289205
1105	42-43	0.25224475817572056
1105	44-45	0.18575041971971729
1105	46-47	0.20153149336151444
1105	48-49	0.22572256975526273
1105	50-51	0.20221751692217538
1105	52-53	0.25569618760167145
1105	54-55	0.25905050451581246
1105	56-57	0.287737082386073
1105	58-59	0.21987451204815045
1105	60-61	0.19579435449560378
1105	62-63	0.1476923229291529
1105	64-65	0.2289205871556632
1105	66-67	0.20028603726728988
1105	68-69	0.15446661773099635
1105	70-71	0.12162669030006157
1105	72-73	0.16631553481646932
1105	74-75	0.20151480840871727
1105	76-77	0.08158910030582689
1105	78-79	0.12026827310098298
1105	80-81	0.14801675283723625
1105	82-83	0.1901684524892744
1105	84-85	0.13313144354490447
1105	86-87	0.1180981220009727
1105	88-89	0.09992167804470142
1105	90-91	0.13410403698495088
1105	92-93	0.0921751717256214
1105	94-95	0.11451432432490805
1105	96-97	0.07557452101089979
1105	98-99	0.035251668887937626
1105	100	0.10865697334196156
1106	1	0.12242141077678781
1106	2	0.13498240009653628
1106	3	0.20158948895898732
1106	4	0.1720713152362876
1106	5	0.12880643200148256
1106	6	0.11707687550646284
1106	7	-0.0022586013884975387
1106	8	0.04981934502323071
1106	9	0.14230367208422479
1106	10-11	0.167117442349749
1106	12-13	0.19904314626163
1106	14-15	0.2736122646382668
1106	16-17	0.21479850939315526
1106	18-19	0.15314037907940303
1106	20-21	0.22451074796698123
1106	22-23	0.24110669583067335
1106	24-25	0.25376715307243103
1106	26-27	0.23443678456901296
1106	28-29	0.32070585333724466
1106	30-31	0.23334425420713245
1106	32-33	0.2083887541314482
1106	34-35	0.2115482365166983
1106	36-37	0.18825310955025998
1106	38-39	0.20065343227926746
1106	40-41	0.25284752629909235
1106	42-43	0.19200750792280985
1106	44-45	0.17700191083649486
1106	46-47	0.13825898707966644
1106	48-49	0.18808579422443472
1106	50-51	0.18553482457609505
1106	52-53	0.1921496362134434
1106	54-55	0.1758461135262337
1106	56-57	0.08229322862172239
1106	58-59	0.12467623743194167
1106	60-61	0.13433139926583237
1106	62-63	0.07019422325468128
1106	64-65	0.07439837131221338
1106	66-67	0.11858473989588703
1106	68-69	0.05011222238982782
1106	70-71	0.06797091410891198
1106	72-73	0.09168980612546562
1106	74-75	0.15524547479220274
1106	76-77	0.09460633019012477
1106	78-79	0.08369746142244594
1106	80-81	0.08832726842430816
1106	82-83	0.11723961768425539
1106	84-85	0.11716262086044793
1106	86-87	0.03159540864750454
1106	88-89	0.09883952050451938
1106	90-91	0.12473802211131613
1106	92-93	0.11284964556300991
1106	94-95	0.11380953710148134
1106	96-97	0.03743065099795473
1106	98-99	0.05091148905965781
1106	100	-0.03926740008957097
1107	1	0.2258502111875167
1107	2	0.2270664471345789
1107	3	0.2186581413673494
1107	4	0.11719748243953632
1107	5	0.15361709333643603
1107	6	0.15552133412737845
1107	7	0.08708630936848749
1107	8	0.11918141525330128
1107	9	0.2484740917807926
1107	10-11	0.2980220754838854
1107	12-13	0.22077316265710323
1107	14-15	0.32495306812334235
1107	16-17	0.28315911694240015
1107	18-19	0.24195192130805765
1107	20-21	0.25131196738973216
1107	22-23	0.293517387878488
1107	24-25	0.24001178405207924
1107	26-27	0.23787937520633307
1107	28-29	0.2726620982088832
1107	30-31	0.19887460874965512
1107	32-33	0.25808017393650573
1107	34-35	0.23513210248638927
1107	36-37	0.2315120667035373
1107	38-39	0.24999985766917376
1107	40-41	0.20423396932215354
1107	42-43	0.22998549589437545
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2216	60-61	-0.5863713345421218
2216	62-63	-0.5734983909309577
2216	64-65	-0.5744636943817696
2216	66-67	-0.547096113123871
2216	68-69	-0.5430254736451019
2216	70-71	-0.55546360072033
2216	72-73	-0.5187280852266412
2216	74-75	-0.5015158120018413
2216	76-77	-0.5715772833326369
2216	78-79	-0.5842030564489704
2216	80-81	-0.5713283841346026
2216	82-83	-0.49307605506491115
2216	84-85	-0.4630447416383774
2216	86-87	-0.48382514445764
2216	88-89	-0.5366938207040022
2216	90-91	-0.4893853178198242
2216	92-93	-0.45039446711052733
2216	94-95	-0.46514666969735075
2216	96-97	-0.4668494889758392
2216	98-99	-0.4952919395635078
2216	100	-0.5653823444144415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	94.0
1	33.0
2	35.0
3	33.0
4	40.0
5	60.0
6	170.0
7	908.0
8	2435.0
9	2852.0
10	2770.0
11	3886.0
12	5569.0
13	6775.0
14	7900.0
15	8464.0
16	9208.0
17	10067.0
18	11225.0
19	12262.0
20	13303.0
21	14927.0
22	16624.0
23	19550.0
24	23974.0
25	30271.0
26	32839.0
27	30587.0
28	32849.0
29	39360.0
30	44628.0
31	50025.0
32	63481.0
33	84473.0
34	126169.0
35	197372.0
36	375205.0
37	758945.0
38	865576.0
39	235811.0
40	327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20978938110212	20.10769064630634	13.505578054375396	24.176941918216144
2	15.001440722621892	20.944031508046553	39.92648334731604	24.128044422015517
3	17.818499521924757	26.292610067949408	30.479326549111846	25.40956386101399
4	12.510904934443037	17.66710817058183	39.50318073854265	30.318806156432476
5	13.405251437417496	38.44430321628731	35.13880017906825	13.011645167226945
6	29.898396135671774	38.3445297856197	18.5953124865676	13.161761592140927
7	26.457416092457564	33.06342878846681	23.071569541675913	17.407585577399715
8	25.800433248305275	34.80708224713492	21.063419554245822	18.329064950313985
9	26.36885410574248	15.638852708034781	20.770222904130943	37.2220702820918
10-11	24.30108016439423	26.44528281942758	29.033710304917644	20.21992671126055
12-13	24.859996048895002	24.440338394154402	28.325745524322937	22.37392003262766
14-15	22.62375719052066	25.651154638341527	25.743409173619053	25.98167899751876
16-17	22.253967637400322	28.377084589653673	26.584555585767383	22.78439218717862
18-19	22.10911194574315	28.257215390094075	27.759609416402657	21.874063247760112
20-21	23.493517796112762	27.442059417641968	26.985800265117753	22.078622521127517
22-23	23.54239188641077	27.134971579133026	26.631390237892237	22.691246296563964
24-25	23.055483081401857	27.274391497012147	27.011972270318708	22.658153151267292
26-27	22.84468205242739	27.5916450979796	26.712639184293717	22.85103366529929
28-29	22.71690008359041	27.693227807669146	26.83126437381772	22.758607734922723
30-31	23.089657442862173	27.350460686107652	26.76799845389931	22.791883417130865
32-33	23.048887285599644	27.605945111152042	26.38662453468748	22.958543068560832
34-35	23.218095975111446	27.50623622827947	26.763986161774028	22.511681634835064
36-37	23.212423816273535	27.312258986698524	26.673446867736565	22.801870329291376
38-39	23.09988371248836	27.45257749422702	26.797250239771348	22.65028855351327
40-41	23.11529139197685	27.085901916630156	26.90785769963624	22.89094899175676
42-43	22.917823990594606	27.075130563653175	26.816017379084627	23.19102806666759
44-45	22.96994642103671	27.489113041262968	26.721381429674622	22.8195591080257
46-47	22.960845338913558	27.277551902990222	26.60043176460135	23.16117099349487
48-49	23.074047122778133	27.149974761910066	26.75216832611752	23.023809789194278
50-51	22.983796167658223	27.25348202543844	26.580619252852745	23.182102554050594
52-53	22.932248578560223	27.287662979128864	26.58617838902006	23.193910053290857
54-55	22.862091544482727	27.303978613590456	26.5924995971258	23.241430244801013
56-57	23.151355715470938	27.124564669387595	26.61430225281104	23.109777362330426
58-59	22.875124982344428	27.171508022357727	26.87304723795189	23.08031975734595
60-61	22.992764600381	27.36343197152657	26.629971341530705	23.01383208656173
62-63	23.067701062542884	27.131331663408503	26.549480002222886	23.251487271825727
64-65	22.726572088991254	27.215816335954784	26.63471649945828	23.422895075595687
66-67	23.004379871223346	26.94763342842561	26.60432987014848	23.44365683020256
68-69	22.778783229333456	27.060626738252342	26.721690920918427	23.438899111495775
70-71	22.840417300388857	26.797582749613557	26.680363718185767	23.681636231811822
72-73	23.010847922709747	26.929979273594917	26.802971124436137	23.2562016792592
74-75	22.883229311609423	27.205850270503195	26.677360250633065	23.233560167254318
76-77	22.815336629940912	26.968087622052266	26.640958796545554	23.575616951461267
78-79	22.883560727661852	27.083651812785035	26.56599531198247	23.46679214757064
80-81	22.78814970288263	27.13739379138961	26.74021280339772	23.334243702330042
82-83	23.055512383291568	26.77621664040236	27.13586460102246	23.03240637528361
84-85	23.21333030149006	26.984742694595294	26.606988398740896	23.19493860517375
86-87	22.70239258586117	27.08894065373706	26.86644451553309	23.34222224486868
88-89	23.019375055730354	26.784183407003553	26.703963542794547	23.49247799447155
90-91	22.923136843767995	26.762584521000953	26.886674980764624	23.427603654466427
92-93	23.09477703310487	26.787126077605254	26.88886153579618	23.229235353493696
94-95	22.594986638062682	26.81668046227112	27.121516079071583	23.466816820594612
96-97	22.718629922390782	26.720889138992938	26.990679855727333	23.56980108288895
98-99	22.672941188459692	26.970136203979067	26.74872651612294	23.6081960914383
100	22.910515942708347	26.61169799628242	26.717136703784107	23.76064935722513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	822.0
1	664.5
2	507.0
3	527.5
4	467.0
5	353.5
6	305.5
7	302.5
8	325.5
9	332.0
10	303.5
11	282.0
12	283.0
13	297.0
14	320.0
15	379.0
16	460.5
17	556.5
18	739.5
19	972.0
20	1309.0
21	1896.0
22	2843.5
23	4132.0
24	5754.0
25	7869.5
26	11367.0
27	15693.5
28	20174.5
29	26112.5
30	32727.0
31	39351.0
32	47340.5
33	54826.5
34	61636.5
35	67891.0
36	75280.5
37	84598.5
38	89059.5
39	90932.5
40	92733.5
41	95019.0
42	98912.5
43	105050.0
44	109998.0
45	116886.0
46	125073.5
47	129001.5
48	130305.5
49	128249.0
50	125278.0
51	125038.5
52	123212.5
53	118938.0
54	113999.0
55	105065.5
56	96282.5
57	85155.5
58	73967.0
59	66065.0
60	54699.5
61	42877.0
62	33916.5
63	25670.0
64	18664.0
65	13137.5
66	9485.5
67	7014.0
68	5120.5
69	3808.5
70	3042.0
71	2345.5
72	1852.5
73	1567.5
74	1220.0
75	878.5
76	631.0
77	435.5
78	256.0
79	164.0
80	112.5
81	68.5
82	52.5
83	41.5
84	34.0
85	32.0
86	22.5
87	14.5
88	15.0
89	13.0
90	9.5
91	8.5
92	6.5
93	7.0
94	7.0
95	5.5
96	5.5
97	5.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060647891395385414
2	0.009455340548256938
3	0.011556527336758481
4	0.009614521365567662
5	0.012097742115614938
6	0.011301838029061323
7	0.0030244355289037344
8	0.005030113827018843
9	0.007163136778982529
10-11	0.006924365553016444
12-13	0.006001116812614253
14-15	0.010028391490575541
16-17	0.009423504384794792
18-19	0.007752105803032204
20-21	0.006797020899167866
22-23	0.002546893076971566
24-25	0.003677076879877698
26-27	0.0046958341106663245
28-29	0.005459902033757794
30-31	0.008834535360745119
32-33	0.010076145735768758
34-35	0.011508773091565264
36-37	0.018003350437842756
38-39	0.018783336442665298
40-41	0.018146613173422407
42-43	0.024052221495650228
44-45	0.027283592087057898
46-47	0.03008517447172662
48-49	0.03142229333713669
50-51	0.03156555607271635
52-53	0.030435372269810215
54-55	0.036420571000693396
56-57	0.03906297256805139
58-59	0.0362454721016516
60-61	0.03732790165936451
62-63	0.032488804813118534
64-65	0.0346377458468133
66-67	0.036595669899735186
68-69	0.03993846706326037
70-71	0.03926990763055534
72-73	0.035847520058374785
74-75	0.03798054301033847
76-77	0.03780544411129668
78-79	0.03699362194301199
80-81	0.04232617932292121
82-83	0.038394413135346354
84-85	0.034319384212191856
86-87	0.03271165795735355
88-89	0.03062638925058308
90-91	0.03291859301985749
92-93	0.034239793803536485
94-95	0.03798054301033847
96-97	0.0336030705342936
98-99	0.031056177457322028
100	0.027665626048603634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3141082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.409253445865836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66670378876951	39.862560042046276
2	14.947725769779494	16.863801027735832
3	6.039079318491324	10.219798675693916
4	3.073226953884118	6.934337525532619
5	1.6625115425462629	4.689051748008476
6	1.0377162544582739	3.512207952157883
7	0.6616863524403106	2.612766320953319
8	0.4427037439050217	1.9978070153097642
9	0.30661291580990974	1.5566225109927414
>10	1.1260915723229965	9.990505586004167
>50	0.02785999589292814	1.058224467013943
>100	0.007988955874130453	0.6737242026236042
>500	9.283582569381951E-5	0.028592925927487134
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5918081731072288E-4
2	0.0	0.0	0.0	0.0	1.5918081731072288E-4
3	0.0	0.0	0.0	0.0	1.5918081731072288E-4
4	0.0	0.0	0.0	0.0	1.5918081731072288E-4
5	0.0	0.0	0.0	0.0	1.9101698077286745E-4
6	0.0	0.0	0.0	0.0	1.9101698077286745E-4
7	0.0	0.0	0.0	0.0	2.2285314423501203E-4
8	0.0	0.0	0.0	0.0	2.2285314423501203E-4
9	0.0	0.0	0.0	3.1836163462144575E-5	2.2285314423501203E-4
10-11	0.0	0.0	0.0	4.775424519321686E-5	2.2285314423501203E-4
12-13	0.0	0.0	0.0	6.367232692428915E-5	6.367232692428915E-4
14-15	0.0	0.0	0.0	1.5918081731072288E-4	7.959040865536144E-4
16-17	0.0	0.0	0.0	1.5918081731072288E-4	9.710029855954094E-4
18-19	0.0	0.0	0.0	1.5918081731072288E-4	0.0010824295577129156
20-21	0.0	0.0	0.0	1.9101698077286745E-4	0.0012893646202168552
22-23	0.0	0.0	0.0	4.775424519321686E-4	0.0013530369471411443
24-25	0.0	0.0	0.0	9.39166822133265E-4	0.0013689550288722167
26-27	0.0	0.0	0.0	0.0019260878894597467	0.0014803816009897226
28-29	0.0	0.0	0.0	0.004982359581825626	0.0015758900913761566
30-31	0.0	0.0	0.0	0.014151174658923262	0.0016236443365693733
32-33	0.0	0.0	0.0	0.03395326833237719	0.0018146613173422408
34-35	0.0	0.0	0.0	0.06464332990988456	0.0019420059711908189
36-37	0.0	0.0	0.0	0.11309797069926861	0.0019738421346529637
38-39	0.0	0.0	0.0	0.1797310608255372	0.002037514461577253
40-41	0.0	0.0	0.0	0.2479718772066441	0.002037514461577253
42-43	0.0	0.0	0.0	0.31594208619832276	0.002053432543308325
44-45	0.0	0.0	0.0	0.399241407897024	0.002164859115425831
46-47	0.0	0.0	0.0	0.49799718695659645	0.0023399580144676264
48-49	0.0	0.0	0.0	0.6021332776412713	0.002483220750047277
50-51	0.0	0.0	0.0	0.7092301315279257	0.002546893076971566
52-53	0.0	0.0	0.0	0.8139233550731881	0.0027379100577444335
54-55	0.0	0.0	0.0	0.9265756194839867	0.002833418548130867
56-57	0.0	0.0	0.0	1.0562920675104948	0.0030085174471726622
58-59	0.0	0.0	0.0	1.1984246192872394	0.003056271692365879
60-61	0.0	3.1836163462144575E-5	0.0	1.3413849113139995	0.0030721897740969514
62-63	0.0	3.1836163462144575E-5	0.0	1.4887226758168044	0.0032472886731387467
64-65	0.0	3.1836163462144575E-5	0.0	1.6383685621706152	0.0032472886731387467
66-67	0.0	3.1836163462144575E-5	0.0	1.8053651576112943	0.0032791248366008914
68-69	0.0	3.1836163462144575E-5	0.0	1.9858125321147297	0.003310961000063036
70-71	0.0	3.1836163462144575E-5	0.0	2.174091602829853	0.0033746333269873245
72-73	0.0	3.1836163462144575E-5	0.0	2.3684036265210526	0.0036134045529534094
74-75	0.0	3.1836163462144575E-5	0.0	2.563686653197847	0.0038840119423816377
76-77	0.0	3.1836163462144575E-5	0.0	2.780395417884665	0.003995438514499143
78-79	0.0	3.1836163462144575E-5	0.0	3.0114145380477173	0.0041705374135409395
80-81	0.0	3.1836163462144575E-5	0.0	3.252350623129227	0.004266045903927373
82-83	0.0	3.1836163462144575E-5	0.0	3.5030126561484227	0.004520735211624529
84-85	0.0	3.1836163462144575E-5	0.0	3.753483672186845	0.004520735211624529
86-87	0.0	3.1836163462144575E-5	0.0	4.017739747004376	0.004727670274128469
88	0.0	3.1836163462144575E-5	0.0	4.226632733561238	0.004902769173170264
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2920	0.0	42.183704	1
GTATCAA	4910	0.0	37.534515	1
CAACGCA	5465	0.0	31.126003	5
ATCAACG	5630	0.0	30.296766	3
TATCAAC	6295	0.0	28.365202	2
AACGCAG	6415	0.0	27.467916	6
TCAACGC	6720	0.0	25.802515	4
GTACATG	10565	0.0	25.32031	1
ACGCAGA	6780	0.0	25.08823	7
TACATGG	10535	0.0	24.799236	2
CGCAGAG	7140	0.0	23.691654	8
ACATGGG	11040	0.0	22.941284	3
GTACTAG	1360	0.0	21.778496	1
TAGTACT	1885	0.0	21.438383	4
CATGGGG	8595	0.0	21.048433	4
GTATAAG	1675	0.0	19.647598	1
GTGGTAT	1495	0.0	19.497402	1
GCAGAGT	8600	0.0	19.06857	9
CTGTGCG	1860	0.0	18.946957	9
AGAGTAC	6895	0.0	18.775682	10-11
>>END_MODULE