FastQCFastQC Report
Mon 16 Jan 2017
ERR1146551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141082
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88290.28108148720727444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80960.2577455793895225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.13603592647374377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.062.4351041
GTATCAA105150.043.996011
ATCAACG132100.034.3127673
CAACGCA133250.034.0160945
TATCAAC136300.033.7674452
AACGCAG144750.031.8511096
TCAACGC143800.031.6512664
ACGCAGA156900.028.8754147
CGCAGAG160900.028.1867778
GCAGAGT180750.024.9092889
GTACATG103350.024.2253571
TACATGG103150.023.76972
GTGGTAT16050.023.7508411
GAGTACT115850.023.30601912-13
GTACTAG13450.022.7436471
CAGAGTA171850.022.56204810-11
AGAGTAC165850.022.37230510-11
ACATGGG107300.022.0419393
TACGGGC10950.021.8993244
ATACGGG11300.021.6374683
Produced by FastQC (version 0.11.5)