FastQCFastQC Report
Mon 16 Jan 2017
ERR1146550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554215
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.037.208711
GTATCAA52000.036.4401931
CAACGCA51500.033.8524785
ATCAACG53600.032.7911073
GTACTAG16550.029.547061
TATCAAC63750.029.117742
AACGCAG64200.028.9113796
ACGCAGA63050.027.2757847
CGTTAAC3700.026.6868021
CGCAGAG64900.026.4993158
GCATCGT17750.025.9433448
TACATGG111100.025.8867862
GTACATG116100.025.109521
ACATGGG113700.024.2206863
TCAACGC75000.023.872864
ACCGTCC1401.9359473E-523.494618
CAATGCG10250.022.92157418-19
CATGGGG74650.022.5998444
CTCGTCG15450.022.5061659
GGGCACC20050.022.50607194
Produced by FastQC (version 0.11.5)