##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146550_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2554215
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151033487783916	31.0	31.0	34.0	28.0	34.0
2	31.184314163059884	31.0	31.0	34.0	28.0	34.0
3	31.437373126381296	33.0	31.0	34.0	28.0	34.0
4	34.97975503236807	37.0	35.0	37.0	32.0	37.0
5	34.96394352080776	37.0	35.0	37.0	32.0	37.0
6	34.92928590584583	37.0	35.0	37.0	32.0	37.0
7	34.9630007654015	37.0	35.0	37.0	32.0	37.0
8	34.88754274796757	37.0	35.0	37.0	32.0	37.0
9	36.47593879137034	39.0	37.0	39.0	32.0	39.0
10-11	36.46315795655417	39.0	37.0	39.0	32.0	39.0
12-13	36.40405604070135	39.0	37.0	39.0	32.0	39.0
14-15	37.74240304751166	40.0	38.0	41.0	32.0	41.0
16-17	37.760832193061276	40.0	38.0	41.0	32.0	41.0
18-19	37.78430789890436	40.0	38.0	41.0	32.0	41.0
20-21	37.888619791207866	40.0	38.0	41.0	32.0	41.0
22-23	37.91413076033145	40.0	38.0	41.0	32.0	41.0
24-25	37.96721399725552	40.0	38.0	41.0	32.0	41.0
26-27	38.00010981847652	40.0	38.0	41.0	32.0	41.0
28-29	37.972885798572165	40.0	38.0	41.0	32.0	41.0
30-31	37.97516027429171	40.0	38.0	41.0	32.0	41.0
32-33	37.98963497591237	40.0	38.0	41.0	32.0	41.0
34-35	37.931359537078905	40.0	38.0	41.0	31.5	41.0
36-37	37.83995454572148	40.0	38.0	41.0	31.0	41.0
38-39	37.78911877034627	40.0	38.0	41.0	31.0	41.0
40-41	37.71790373950509	40.0	37.5	41.0	31.0	41.0
42-43	37.58667026855609	40.0	37.0	41.0	30.5	41.0
44-45	37.41304490812246	40.0	37.0	41.0	30.0	41.0
46-47	37.30162613562288	40.0	36.5	41.0	30.0	41.0
48-49	37.17381113179587	40.0	36.0	41.0	30.0	41.0
50-51	37.0261350356176	40.0	36.0	41.0	30.0	41.0
52-53	36.88620476349877	39.0	35.0	41.0	30.0	41.0
54-55	36.70527598498951	39.0	35.0	41.0	30.0	41.0
56-57	36.5536468151663	39.0	35.0	41.0	30.0	41.0
58-59	36.309519363091994	38.5	35.0	40.5	29.5	41.0
60-61	36.14044726070436	38.0	35.0	40.0	29.5	41.0
62-63	35.902587291985995	37.5	35.0	40.0	29.0	41.0
64-65	35.65263378376527	37.0	34.5	40.0	29.0	41.0
66-67	35.4081700248413	36.5	34.0	40.0	29.0	41.0
68-69	35.11991238012462	36.0	34.0	39.0	29.0	41.0
70-71	34.90804532899541	36.0	34.0	39.0	29.0	41.0
72-73	34.61601959897659	35.0	34.0	39.0	28.5	40.5
74-75	34.3130574364335	35.0	34.0	37.5	28.0	40.0
76-77	34.030728814919655	35.0	34.0	37.0	28.0	39.0
78-79	33.764999618278026	35.0	34.0	37.0	28.0	39.0
80-81	33.520473805063396	35.0	33.0	36.0	27.5	39.0
82-83	33.26839439906194	35.0	33.0	36.0	27.0	37.0
84-85	33.04942751491163	35.0	33.0	35.5	27.0	37.0
86-87	32.86472654024818	35.0	33.0	35.0	27.0	37.0
88-89	32.73845623802225	35.0	33.0	35.0	27.0	36.0
90-91	32.57058665774025	35.0	33.0	35.0	26.5	36.0
92-93	32.46945676068773	35.0	33.0	35.0	26.0	36.0
94-95	32.3838950519044	35.0	33.0	35.0	26.0	36.0
96-97	32.30283570490346	35.0	33.0	35.0	26.0	35.0
98-99	32.23242326898871	35.0	33.0	35.0	25.0	35.0
100	31.25922602443412	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4654956393937155
1101	2	0.43398606976413845
1101	3	0.4917068949977583
1101	4	0.4978883770532576
1101	5	0.49292592778949285
1101	6	0.4628933366908825
1101	7	0.47744477429863963
1101	8	0.5454005210464601
1101	9	0.6839446649044305
1101	10-11	0.7268453533294519
1101	12-13	0.7230184985840964
1101	14-15	0.7956769554385659
1101	16-17	0.7592653249057335
1101	18-19	0.6830837740913367
1101	20-21	0.6600635509662851
1101	22-23	0.6941495692984319
1101	24-25	0.7093662663422862
1101	26-27	0.6982299495978381
1101	28-29	0.7950463347697223
1101	30-31	0.7976956011497194
1101	32-33	0.750802140147492
1101	34-35	0.779160188117018
1101	36-37	0.7852316661866183
1101	38-39	0.7030061100989329
1101	40-41	0.7275330855904727
1101	42-43	0.6939983608178437
1101	44-45	0.6838590799499897
1101	46-47	0.7071340693558383
1101	48-49	0.7214803912606556
1101	50-51	0.7225237890112268
1101	52-53	0.6347396746181033
1101	54-55	0.6273631517063194
1101	56-57	0.6560296959577414
1101	58-59	0.6547814419872466
1101	60-61	0.6461805014562714
1101	62-63	0.6178250392774416
1101	64-65	0.5188275945346348
1101	66-67	0.5204132439295179
1101	68-69	0.4544915118553732
1101	70-71	0.5247129500862968
1101	72-73	0.5396819999037774
1101	74-75	0.5004855576046197
1101	76-77	0.5030849599492768
1101	78-79	0.48308473909445127
1101	80-81	0.4608205339803959
1101	82-83	0.5146269774843759
1101	84-85	0.5027003275178501
1101	86-87	0.47827154639075076
1101	88-89	0.45870194993820235
1101	90-91	0.45206785843068786
1101	92-93	0.5110686106387661
1101	94-95	0.4785290270058269
1101	96-97	0.3840023566486792
1101	98-99	0.40032871943842707
1101	100	0.3812115427843956
1102	1	0.31066138988072467
1102	2	0.32898782103218593
1102	3	0.35530863760633835
1102	4	0.33244164533724785
1102	5	0.39592176703268933
1102	6	0.4136624878894182
1102	7	0.3188546065694524
1102	8	0.4073662305610455
1102	9	0.44393097822658945
1102	10-11	0.4871105723036635
1102	12-13	0.4741793559658447
1102	14-15	0.5644258003513514
1102	16-17	0.6035005615644664
1102	18-19	0.5433677524399698
1102	20-21	0.5337216491953285
1102	22-23	0.5599701323020483
1102	24-25	0.5178579452498582
1102	26-27	0.6203867587621801
1102	28-29	0.5944838146688909
1102	30-31	0.5841032761345843
1102	32-33	0.5990206481951574
1102	34-35	0.5721025610372124
1102	36-37	0.5752670099599442
1102	38-39	0.6120935655106763
1102	40-41	0.5890725184356995
1102	42-43	0.4923831424585501
1102	44-45	0.4459864637678024
1102	46-47	0.48614282636996364
1102	48-49	0.5073166929329602
1102	50-51	0.42625421739042935
1102	52-53	0.42173169527144694
1102	54-55	0.4288898126775109
1102	56-57	0.466083785627724
1102	58-59	0.5164613384914318
1102	60-61	0.4496383166183904
1102	62-63	0.4637371214096362
1102	64-65	0.47830077536809057
1102	66-67	0.42658293009453985
1102	68-69	0.35558092019633136
1102	70-71	0.41737845586749955
1102	72-73	0.4043009352642031
1102	74-75	0.3298912855567906
1102	76-77	0.47402288149819327
1102	78-79	0.44123958319958234
1102	80-81	0.47182298710343673
1102	82-83	0.4303329878464268
1102	84-85	0.44211369736695616
1102	86-87	0.44431122427872793
1102	88-89	0.36450449316015465
1102	90-91	0.41678182893357274
1102	92-93	0.3412461516495213
1102	94-95	0.34950395346351826
1102	96-97	0.31172232695040947
1102	98-99	0.3213028540769969
1102	100	0.36146972299198055
1103	1	0.15138503672609005
1103	2	0.2020764100543886
1103	3	0.2706153675503735
1103	4	0.23833194235267996
1103	5	0.2701722656403689
1103	6	0.2347232752223789
1103	7	0.1617287359933428
1103	8	0.26178811552066605
1103	9	0.44734471440959567
1103	10-11	0.3720818494682163
1103	12-13	0.4006585098354094
1103	14-15	0.4397700887312652
1103	16-17	0.323392908615304
1103	18-19	0.3005068393197661
1103	20-21	0.3209232611767163
1103	22-23	0.27709292586827416
1103	24-25	0.36093830934476046
1103	26-27	0.2915967981958474
1103	28-29	0.30880061003892223
1103	30-31	0.3067939573930545
1103	32-33	0.37291566003111853
1103	34-35	0.37293355753346447
1103	36-37	0.40485259853062416
1103	38-39	0.33693093811928065
1103	40-41	0.31024915429367894
1103	42-43	0.24086665110426964
1103	44-45	0.23633361309833134
1103	46-47	0.2085731358467129
1103	48-49	0.1706704918334907
1103	50-51	0.2137123283933562
1103	52-53	0.20432786161391903
1103	54-55	0.18652514281686194
1103	56-57	0.13848133562994747
1103	58-59	0.1186297254517612
1103	60-61	0.14296409956412504
1103	62-63	0.1460351178310475
1103	64-65	0.1125099340953355
1103	66-67	0.10745999042333665
1103	68-69	0.12459042468359627
1103	70-71	0.09867552722918305
1103	72-73	0.1101326353798413
1103	74-75	0.05499351419575049
1103	76-77	0.05535080449043761
1103	78-79	0.13645029967325684
1103	80-81	0.09937772469579897
1103	82-83	0.07530144890284873
1103	84-85	0.0887349301700695
1103	86-87	0.08310397812430637
1103	88-89	-0.029856596802517288
1103	90-91	0.07023530371261444
1103	92-93	0.08824468097903804
1103	94-95	0.019049562104065387
1103	96-97	0.02142519737503079
1103	98-99	0.02189499503177217
1103	100	-0.019528265292549918
1104	1	0.24155349544184546
1104	2	0.23748079422239243
1104	3	0.2416197163964
1104	4	0.2467769735224863
1104	5	0.24688825159950056
1104	6	0.2506454296301115
1104	7	0.18000111913232786
1104	8	0.21314587866230283
1104	9	0.26146557055854913
1104	10-11	0.2923792111876935
1104	12-13	0.2754638158212259
1104	14-15	0.3196021898672967
1104	16-17	0.30447068382401454
1104	18-19	0.29074837745857707
1104	20-21	0.3088120049160068
1104	22-23	0.295136849649694
1104	24-25	0.29177459389866556
1104	26-27	0.323021715981497
1104	28-29	0.30061662604317974
1104	30-31	0.29943945012275464
1104	32-33	0.30598162520067973
1104	34-35	0.29521402765886506
1104	36-37	0.3306842981970206
1104	38-39	0.2694387364105779
1104	40-41	0.3343133612528888
1104	42-43	0.33937820354265114
1104	44-45	0.26780884861710774
1104	46-47	0.2948830209377107
1104	48-49	0.29995486108538216
1104	50-51	0.3202997238241849
1104	52-53	0.32353497514749563
1104	54-55	0.3632658357785914
1104	56-57	0.34871296033287535
1104	58-59	0.38619878998689927
1104	60-61	0.39852641095400543
1104	62-63	0.3781840212529346
1104	64-65	0.30217699995955627
1104	66-67	0.3202088420570206
1104	68-69	0.32378354881514326
1104	70-71	0.31212752514570496
1104	72-73	0.314538623052222
1104	74-75	0.29313417209589687
1104	76-77	0.2705776321595579
1104	78-79	0.33513867643774375
1104	80-81	0.30921186228317055
1104	82-83	0.30583551305083034
1104	84-85	0.3407813569715188
1104	86-87	0.27527280032249024
1104	88-89	0.2541966183742588
1104	90-91	0.28946396120102946
1104	92-93	0.38761397844621825
1104	94-95	0.295929321945259
1104	96-97	0.2806291876861451
1104	98-99	0.24198701904305864
1104	100	0.20118660341377748
1105	1	0.2018540852751869
1105	2	0.13088837578871093
1105	3	0.18005538596105808
1105	4	0.1562975767491679
1105	5	0.10285918103297575
1105	6	0.13003014016727832
1105	7	0.018747204212203883
1105	8	0.14503628429335436
1105	9	0.15435803490390043
1105	10-11	0.2229485597391374
1105	12-13	0.21577177491597865
1105	14-15	0.29052449533488556
1105	16-17	0.3048732882145728
1105	18-19	0.26095543323393855
1105	20-21	0.24055952144566106
1105	22-23	0.25385886034682414
1105	24-25	0.23644664714450414
1105	26-27	0.3416626209385001
1105	28-29	0.39510558092399606
1105	30-31	0.30956067008643373
1105	32-33	0.30278385557198106
1105	34-35	0.3251183880976285
1105	36-37	0.3021011776072413
1105	38-39	0.2728926390485924
1105	40-41	0.24360341918347217
1105	42-43	0.2797392388654032
1105	44-45	0.1409400317052345
1105	46-47	0.122354465049753
1105	48-49	0.1806967073778054
1105	50-51	0.14035785910861875
1105	52-53	0.07462004040347381
1105	54-55	0.17431735188715436
1105	56-57	0.2236892734212077
1105	58-59	0.19833913640066925
1105	60-61	0.22946721999363007
1105	62-63	0.13382494229364283
1105	64-65	0.15621316928881157
1105	66-67	0.09841066916595764
1105	68-69	0.1786167023034082
1105	70-71	0.1284125693466578
1105	72-73	0.14540537393902042
1105	74-75	0.1996976033058857
1105	76-77	0.15237087758812606
1105	78-79	0.16024497510922942
1105	80-81	0.21822407420206957
1105	82-83	0.1875419430238452
1105	84-85	0.21329741143386372
1105	86-87	0.23610513939039635
1105	88-89	0.1411684514195457
1105	90-91	0.12676806897940196
1105	92-93	0.1865762504596873
1105	94-95	0.20400045432585046
1105	96-97	0.16818281222227682
1105	98-99	0.1166734233406217
1105	100	0.1736170852523493
1106	1	0.20918554253368526
1106	2	0.19980198949100725
1106	3	0.21420168505602533
1106	4	0.2007062657550236
1106	5	0.2015402830532267
1106	6	0.21982246910573622
1106	7	0.24814686026515176
1106	8	0.2477127308686491
1106	9	0.31533915255810996
1106	10-11	0.23526073511739298
1106	12-13	0.31844691866152175
1106	14-15	0.2894225185078767
1106	16-17	0.27111740376688687
1106	18-19	0.2551652235652071
1106	20-21	0.2312627455150107
1106	22-23	0.320424557708165
1106	24-25	0.2921513043265662
1106	26-27	0.34616628623285095
1106	28-29	0.3998042364036962
1106	30-31	0.3215217328480833
1106	32-33	0.298607634820506
1106	34-35	0.29624116677290857
1106	36-37	0.30023270261288815
1106	38-39	0.2601301188547325
1106	40-41	0.24333568767062275
1106	42-43	0.20203673569518088
1106	44-45	0.15237996256840347
1106	46-47	0.0664242011833025
1106	48-49	0.13958309193839824
1106	50-51	0.1760892677281376
1106	52-53	0.22375756711148398
1106	54-55	0.22693147977291517
1106	56-57	0.13358252823734063
1106	58-59	0.1691726868722938
1106	60-61	0.21462885773748042
1106	62-63	0.19678940109815812
1106	64-65	0.23080994348236317
1106	66-67	0.26215508871187865
1106	68-69	0.2560056577813725
1106	70-71	0.13887342365554645
1106	72-73	0.18881696391244418
1106	74-75	0.13904023884361294
1106	76-77	0.1555228570207845
1106	78-79	0.2086194778625412
1106	80-81	0.18592562196381834
1106	82-83	0.1916764732234384
1106	84-85	0.2631656084912066
1106	86-87	0.15324842460588428
1106	88-89	0.16390847608646197
1106	90-91	0.2627018504861667
1106	92-93	0.1604794849516722
1106	94-95	0.1776678766337696
1106	96-97	0.08242491273124841
1106	98-99	0.165903668098899
1106	100	0.1697676576288849
1107	1	0.13487760013359917
1107	2	0.202842667068456
1107	3	0.20174715715997493
1107	4	0.16410894851454572
1107	5	0.09708108046930874
1107	6	0.10271699681116786
1107	7	0.10443090389818366
1107	8	0.09898737943309754
1107	9	0.17909201642114425
1107	10-11	0.2627826235387971
1107	12-13	0.16007469734101676
1107	14-15	0.2831635078339758
1107	16-17	0.14979178944320637
1107	18-19	0.07483667228394353
1107	20-21	0.0925221948964321
1107	22-23	0.1350643956582971
1107	24-25	0.19644032203380846
1107	26-27	0.22732777909424584
1107	28-29	0.19265033248149166
1107	30-31	0.20398834552429435
1107	32-33	0.2278350022082023
1107	34-35	0.20601809311236963
1107	36-37	0.14318312137532985
1107	38-39	0.1943658300949238
1107	40-41	0.0821992586040281
1107	42-43	0.14828104896654537
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2216	48-49	-0.632760086911432
2216	50-51	-0.6757801712148606
2216	52-53	-0.5420668719103787
2216	54-55	-0.47797471781786527
2216	56-57	-0.5187152346095658
2216	58-59	-0.5474891355208698
2216	60-61	-0.4856330334518759
2216	62-63	-0.5097013245589892
2216	64-65	-0.5182602564246608
2216	66-67	-0.5387899576992652
2216	68-69	-0.4681732378594603
2216	70-71	-0.599064523078475
2216	72-73	-0.5213478991072762
2216	74-75	-0.5279172719959178
2216	76-77	-0.5815960695182483
2216	78-79	-0.6004016108747834
2216	80-81	-0.544845240895178
2216	82-83	-0.520732715424586
2216	84-85	-0.446841346233235
2216	86-87	-0.46780133321799156
2216	88-89	-0.530560001962975
2216	90-91	-0.5143830835228087
2216	92-93	-0.529818787107228
2216	94-95	-0.5076402304269294
2216	96-97	-0.5144138045051285
2216	98-99	-0.5041051717614309
2216	100	-0.5630665277708502
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	73.0
1	36.0
2	33.0
3	27.0
4	26.0
5	37.0
6	95.0
7	436.0
8	1473.0
9	1979.0
10	1810.0
11	2418.0
12	3628.0
13	4718.0
14	5872.0
15	6471.0
16	7092.0
17	7753.0
18	8773.0
19	9430.0
20	10613.0
21	11765.0
22	13248.0
23	15835.0
24	20034.0
25	26030.0
26	28237.0
27	26371.0
28	26656.0
29	32840.0
30	37828.0
31	41476.0
32	51608.0
33	68598.0
34	107041.0
35	166872.0
36	321361.0
37	628778.0
38	661153.0
39	195536.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41704349270434	21.483530452884633	13.33049448795208	23.76893156645895
2	14.789622471593356	21.420018950814026	40.56547036390262	23.224888213690008
3	17.47009204285649	27.662936843337633	30.66358016836086	24.20339094544502
4	11.971999736880848	18.10851002515294	41.04580465908842	28.873685578877794
5	12.5729912216713	39.53662296492858	35.45506622608611	12.435319587314005
6	28.189690918637723	39.53200625077577	19.198307858146077	13.079994972440437
7	25.466499721235586	33.447266542632164	24.072246966479362	17.01398676965289
8	25.244580314783978	35.05167636426424	21.415474679814835	18.288268641136945
9	26.33943219147778	15.299389592134782	21.594617134489415	36.76656108189802
10-11	24.14501247734706	27.121173471635107	28.88066832457038	19.853145726447455
12-13	24.687775481426275	25.0679753325256	28.24240231647639	22.001846869571736
14-15	22.60002623382166	26.154160899467687	25.905194100914073	25.34061876579658
16-17	21.7774501829017	28.69135961988553	26.90327891799719	22.627911279215574
18-19	21.358575168342853	28.95254293829243	28.290917747514303	21.397964145850416
20-21	23.119656479881975	28.104840667369345	27.198566343047137	21.57693650970154
22-23	23.340934713288203	27.651267558982145	26.880343160314336	22.127454567415313
24-25	22.679495918540738	27.952622262697275	27.443717207278773	21.924164611483214
26-27	22.547197846277086	28.267652380163728	26.738751077194255	22.446398696364927
28-29	22.055767539261687	28.42232635754568	27.243217973347022	22.278688129845616
30-31	22.612189762087716	27.974627910277555	27.20671107434943	22.206471253285304
32-33	22.72890122010955	28.346471799967972	26.538821614040366	22.385805365882117
34-35	22.742486505958524	28.37061284581846	27.179965582118363	21.706935066104652
36-37	22.595102127603752	27.86883480571546	26.98716765185513	22.548895414825658
38-39	22.605405318621756	27.956411133459724	27.424227858475785	22.01395568944274
40-41	22.472063445943643	27.17374917424805	27.884786702446966	22.469400677361335
42-43	22.3187967651727	27.49726560679641	27.333965647484337	22.84997198054655
44-45	22.450622404039027	28.092771998749942	27.277846833701062	22.17875876350996
46-47	22.38316518979323	27.559294413950525	26.999536112987133	23.05800428326911
48-49	22.808171497667313	27.406768459546388	27.336313035134697	22.4487470076516
50-51	22.70308269977334	27.32549726096471	27.05886845287342	22.912551586388535
52-53	22.28613210037354	27.661604701455538	27.113634174061318	22.93862902410961
54-55	22.36776580247201	27.576785553077464	27.03933612805729	23.016112516393235
56-57	22.99506323752057	27.32311696948701	26.83466947152221	22.84715032147021
58-59	22.31961174395044	27.469256502511392	27.56135432163582	22.649777431902347
60-61	22.47620625443431	27.814002928430863	27.07858569753112	22.631205119603703
62-63	22.690245779925796	27.366473568274607	26.815709408949733	23.127571242849868
64-65	21.929553154915933	27.46125505766848	27.22119327098528	23.3879985164303
66-67	22.798243729613006	27.158194169277877	26.92791916503329	23.115642936075822
68-69	22.187111392241455	27.285762684646826	27.306423153825516	23.220702769286206
70-71	22.230484217782458	26.984145324084775	27.199934199961618	23.58543625817115
72-73	22.682546878381064	27.023018408656306	27.384004707665525	22.9104300052971
74-75	22.61598449173073	27.48574005710831	27.08148558378376	22.816789867377192
76-77	22.380130920262484	27.430941980042174	26.973796168662208	23.21513093103314
78-79	22.350387799711037	27.360451440144352	26.972122991433274	23.31703776871134
80-81	22.14060599461487	27.414128829130703	27.39415343802919	23.05111173822524
82-83	22.805112830897883	26.809443043274513	28.022262837546343	22.363181288281265
84-85	22.89124138619794	27.20199035371664	27.035070095150814	22.871698164934603
86-87	21.92201354023248	27.58832648906624	27.483953945272365	23.005706025428914
88-89	22.708682621011658	26.729676499396298	27.304358757755804	23.25728212183624
90-91	22.685634122308052	26.748248187875095	27.37105597496001	23.19506171485684
92-93	22.92860420117663	26.906717304234878	27.368843208723113	22.79583528586538
94-95	21.888556411120074	27.007856745599675	27.765860364559263	23.337726478720988
96-97	22.200303172185233	26.99640411391457	27.4812407070049	23.322052006895298
98-99	21.903621129771185	27.320923223213576	27.364864600247042	23.410591046768197
100	22.4768775702739	26.72477837047817	26.95763769579706	23.840706363450874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	883.0
1	649.0
2	403.0
3	383.0
4	343.0
5	284.0
6	250.5
7	239.5
8	229.5
9	214.0
10	186.5
11	167.5
12	183.0
13	206.0
14	218.0
15	259.0
16	310.5
17	368.5
18	447.5
19	568.0
20	789.5
21	1136.0
22	1696.0
23	2513.5
24	3430.0
25	4753.0
26	7260.0
27	10210.0
28	13424.5
29	19062.5
30	25855.0
31	32195.0
32	41006.0
33	50087.5
34	57933.5
35	65014.5
36	73778.5
37	85469.0
38	88926.0
39	87038.5
40	85091.5
41	81091.0
42	79697.5
43	82247.5
44	86494.5
45	97593.0
46	105987.0
47	109408.0
48	113002.5
49	109502.5
50	104167.5
51	104497.5
52	104428.0
53	100068.5
54	93634.5
55	81281.0
56	71893.5
57	60030.0
58	47242.5
59	42623.0
60	34776.0
61	24471.5
62	17209.0
63	13386.0
64	9401.5
65	5071.5
66	3432.5
67	2427.0
68	1625.5
69	1167.5
70	826.5
71	570.0
72	434.5
73	344.0
74	244.5
75	187.5
76	148.5
77	112.5
78	98.5
79	83.0
80	55.5
81	45.0
82	36.5
83	23.0
84	15.5
85	17.0
86	19.5
87	13.0
88	7.0
89	6.5
90	5.5
91	4.5
92	6.0
93	6.0
94	4.0
95	3.0
96	2.0
97	3.5
98	4.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06005759107984254
2	0.009200478424878094
3	0.011588687718144322
4	0.009357082312961125
5	0.012215103270476447
6	0.01143208383006129
7	0.0034061345658059324
8	0.005716041915030645
9	0.007242929823840201
10-11	0.007301656281871338
12-13	0.006401183925393907
14-15	0.01017925272539704
16-17	0.009689865575137567
18-19	0.007869345376172326
20-21	0.0073212317678817175
22-23	0.0028775964435257015
24-25	0.004071701090158816
26-27	0.004933022474615488
28-29	0.00651863684145618
30-31	0.00890684613472241
32-33	0.010335856613480071
34-35	0.012136801326434932
36-37	0.01853798525182884
38-39	0.019164400804160965
40-41	0.018870768514005282
42-43	0.024743414317118958
44-45	0.02854105860313247
46-47	0.030987994354429834
48-49	0.03239742934717712
50-51	0.033376203647696064
52-53	0.03104672081246097
54-55	0.03725214987775109
56-57	0.040071019863245655
58-59	0.03725214987775109
60-61	0.03799601834614549
62-63	0.03341535461971682
64-65	0.03578398842697267
66-67	0.037721961542000186
68-69	0.040325501181380584
70-71	0.04012974632127679
72-73	0.03650828140935669
74-75	0.039013943618685196
76-77	0.03842667903837382
78-79	0.037741537028010565
80-81	0.04202856846428355
82-83	0.0388181887585814
84-85	0.03523587481868206
86-87	0.033728562395882886
88-89	0.0314773815046893
90-91	0.033630684965830986
92-93	0.03549035613681699
94-95	0.03895521716065405
96-97	0.03447243086427728
98-99	0.03182974025287613
100	0.029050021239402323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2554215.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.30707863964794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.70879122735036	38.399878856981644
2	14.58712571378095	15.84368366529465
3	6.049023741663016	9.855144240947723
4	3.0027827109106884	6.522894272768079
5	1.6913399051884617	4.592586461872225
6	1.093064503344047	3.5616683964787716
7	0.7067750587442595	2.6868022087036403
8	0.4676099068481856	2.031562238710629
9	0.33591813044760155	1.6418459094031272
>10	1.2943618194401687	11.24584081676555
>50	0.043891210567300463	1.6626187107697872
>100	0.018471054813531562	1.6634801661720684
>500	7.243002013357656E-4	0.21758605051269914
>1k	1.2071670022262762E-4	0.07440800461943764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.9575486010378923E-5	0.0
12-13	0.0	0.0	0.0	3.9150972020757845E-5	0.0
14-15	0.0	0.0	0.0	3.9150972020757845E-5	0.0
16-17	0.0	0.0	0.0	3.9150972020757845E-5	0.0
18-19	0.0	0.0	0.0	3.9150972020757845E-5	0.0
20-21	0.0	0.0	0.0	1.1745291606227355E-4	0.0
22-23	0.0	0.0	0.0	5.08962636269852E-4	0.0
24-25	0.0	0.0	0.0	0.0013115575626953878	0.0
26-27	0.0	0.0	0.0	0.002525237695338881	0.0
28-29	0.0	0.0	0.0	0.006342457467362771	0.0
30-31	0.0	0.0	0.0	0.017578786437320273	0.0
32-33	0.0	0.0	0.0	0.04195026652024203	0.0
34-35	0.0	0.0	0.0	0.08221704124359147	0.0
36-37	0.0	0.0	0.0	0.14503477585089744	0.0
38-39	0.0	0.0	0.0	0.22932681861158907	0.0
40-41	0.0	0.0	0.0	0.3153806551132148	0.0
42-43	0.0	0.0	0.0	0.4020804826531831	0.0
44-45	0.0	0.0	0.0	0.5092171175879869	0.0
46-47	0.0	0.0	0.0	0.6326209813974156	0.0
48-49	0.0	0.0	0.0	0.7614668303177297	0.0
50-51	0.0	0.0	0.0	0.8926225865872686	0.0
52-53	0.0	0.0	0.0	1.02236890786406	0.0
54-55	0.0	0.0	0.0	1.1635277374849022	0.0
56-57	0.0	0.0	0.0	1.3211887018124941	0.0
58-59	0.0	0.0	0.0	1.4987579354126415	0.0
60-61	0.0	0.0	0.0	1.6800856623267815	0.0
62-63	0.0	0.0	0.0	1.86186362541916	0.0
64-65	0.0	0.0	0.0	2.049240177510507	0.0
66-67	0.0	0.0	0.0	2.2535886759728525	0.0
68-69	0.0	0.0	0.0	2.4796855393927295	0.0
70-71	0.0	0.0	0.0	2.7179583551110618	0.0
72-73	0.0	0.0	0.0	2.9583648988045255	0.0
74-75	0.0	0.0	0.0	3.198125451459646	0.0
76-77	0.0	0.0	0.0	3.4551320072899108	0.0
78-79	0.0	0.0	0.0	3.7349635798082774	0.0
80-81	0.0	0.0	0.0	4.034820874515262	0.0
82-83	0.0	0.0	0.0	4.338260483162146	0.0
84-85	0.0	0.0	0.0	4.640995374312656	0.0
86-87	0.0	1.9575486010378923E-5	0.0	4.96154395773261	0.0
88	0.0	7.830194404151569E-5	0.0	5.2105637152706406	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3235	0.0	37.20871	1
GTATCAA	5200	0.0	36.440193	1
CAACGCA	5150	0.0	33.852478	5
ATCAACG	5360	0.0	32.791107	3
GTACTAG	1655	0.0	29.54706	1
TATCAAC	6375	0.0	29.11774	2
AACGCAG	6420	0.0	28.911379	6
ACGCAGA	6305	0.0	27.275784	7
CGTTAAC	370	0.0	26.686802	1
CGCAGAG	6490	0.0	26.499315	8
GCATCGT	1775	0.0	25.943344	8
TACATGG	11110	0.0	25.886786	2
GTACATG	11610	0.0	25.10952	1
ACATGGG	11370	0.0	24.220686	3
TCAACGC	7500	0.0	23.87286	4
ACCGTCC	140	1.9359473E-5	23.49461	8
CAATGCG	1025	0.0	22.921574	18-19
CATGGGG	7465	0.0	22.599844	4
CTCGTCG	1545	0.0	22.506165	9
GGGCACC	2005	0.0	22.506071	94
>>END_MODULE