FastQCFastQC Report
Mon 16 Jan 2017
ERR1146550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554215
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86150.33728562395882883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79010.3093318299360077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.15805247404779943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.055.8599051
GTATCAA106150.043.083551
CAACGCA126250.035.1939355
ATCAACG126850.035.0274663
TATCAAC135800.033.3970762
AACGCAG139150.032.491686
TCAACGC146500.030.6180924
GTGGTAT13050.030.2854461
ACGCAGA149700.029.6355887
CGCAGAG151450.029.3241798
GCAGAGT165250.026.5909259
TGATCGC12350.026.257029
GTACTAG16350.025.6116431
CGTTAAC5300.024.856921
GTATAAG15150.024.8452031
GAGTACT103250.024.55639812-13
TACATGG108700.024.3674452
GTACATG111050.023.9807681
ACATGGG109050.023.8864443
GTGATCG13400.023.8488488
Produced by FastQC (version 0.11.5)