##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146550_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2554215 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4510360325971 34.0 31.0 34.0 31.0 34.0 2 32.48685055878225 34.0 31.0 34.0 31.0 34.0 3 32.630655602601976 34.0 31.0 34.0 31.0 34.0 4 36.14876351442616 37.0 35.0 37.0 35.0 37.0 5 36.19209815931705 37.0 37.0 37.0 35.0 37.0 6 36.28460564204658 37.0 37.0 37.0 35.0 37.0 7 36.26517892972988 37.0 37.0 37.0 35.0 37.0 8 36.27733530654233 37.0 37.0 37.0 35.0 37.0 9 38.085419199245166 39.0 38.0 39.0 37.0 39.0 10-11 38.05975123472378 39.0 38.0 39.0 36.0 39.0 12-13 38.040081786380554 39.0 38.0 39.0 35.0 39.0 14-15 39.47310132467314 41.0 39.0 41.0 36.5 41.0 16-17 39.469017878291375 41.0 39.0 41.0 36.5 41.0 18-19 39.5250501621829 41.0 39.5 41.0 37.0 41.0 20-21 39.56026450396698 41.0 40.0 41.0 37.0 41.0 22-23 39.519142672014695 41.0 39.0 41.0 36.5 41.0 24-25 39.49820277462939 41.0 39.0 41.0 36.0 41.0 26-27 39.419631471900374 41.0 39.0 41.0 36.0 41.0 28-29 39.33298077883029 41.0 39.0 41.0 36.0 41.0 30-31 39.2319203747531 41.0 39.0 41.0 35.5 41.0 32-33 39.102474341431716 41.0 39.0 41.0 35.0 41.0 34-35 39.0237337107487 41.0 39.0 41.0 35.0 41.0 36-37 38.94444927306432 41.0 39.0 41.0 35.0 41.0 38-39 38.83198379932777 40.0 38.0 41.0 35.0 41.0 40-41 38.73249080441545 40.0 38.0 41.0 35.0 41.0 42-43 38.62385840659459 40.0 38.0 41.0 35.0 41.0 44-45 38.43856037960782 40.0 38.0 41.0 35.0 41.0 46-47 38.15709640731105 40.0 38.0 41.0 34.0 41.0 48-49 37.89767130018421 40.0 37.5 41.0 33.5 41.0 50-51 37.72028920823031 40.0 37.0 41.0 33.0 41.0 52-53 37.576604357894695 40.0 37.0 41.0 33.0 41.0 54-55 37.42820161967571 40.0 36.0 41.0 33.0 41.0 56-57 37.19747397928522 39.5 36.0 41.0 33.0 41.0 58-59 36.958140955244566 39.0 35.0 41.0 32.0 41.0 60-61 36.76800974076184 39.0 35.0 41.0 32.0 41.0 62-63 36.505413209146454 38.5 35.0 41.0 32.0 41.0 64-65 36.215741627075246 37.5 35.0 41.0 31.5 41.0 66-67 35.97180366570551 37.0 35.0 40.0 31.0 41.0 68-69 35.674263521277574 36.5 35.0 40.0 31.0 41.0 70-71 35.43628805719174 36.0 35.0 39.0 31.0 41.0 72-73 35.14050833622072 36.0 35.0 39.0 31.0 41.0 74-75 34.82927200725076 35.0 35.0 38.5 31.0 40.5 76-77 34.54877682575665 35.0 35.0 37.0 30.5 39.5 78-79 34.27819388735874 35.0 35.0 37.0 30.0 39.0 80-81 34.04948252202732 35.0 35.0 36.5 30.0 39.0 82-83 33.80737702190301 35.0 34.0 36.0 30.0 37.5 84-85 33.605532815365976 35.0 34.0 36.0 30.0 37.0 86-87 33.43697731005417 35.0 34.0 35.5 30.0 37.0 88-89 33.30328966042404 35.0 34.0 35.0 30.0 36.0 90-91 33.216513096978915 35.0 34.0 35.0 29.5 36.0 92-93 33.12597921474895 35.0 34.0 35.0 29.0 36.0 94-95 33.06004271371047 35.0 34.0 35.0 29.0 36.0 96-97 33.018166834037075 35.0 34.0 35.0 29.0 35.5 98-99 32.97353883678547 35.0 34.0 35.0 29.0 35.0 100 32.42972655003592 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3240171478313272 1101 2 0.3064103716848692 1101 3 0.32670055705619916 1101 4 0.1980547295780397 1101 5 0.23416161811807257 1101 6 0.17113029794318635 1101 7 0.19470564763564369 1101 8 0.17213204475300614 1101 9 0.25594639254052254 1101 10-11 0.2748638952531337 1101 12-13 0.22949062662193143 1101 14-15 0.3702285369635021 1101 16-17 0.3951744598516953 1101 18-19 0.33849224887504903 1101 20-21 0.35128737853434444 1101 22-23 0.40224326628195683 1101 24-25 0.4042069891871378 1101 26-27 0.3702838164754212 1101 28-29 0.41178425658092266 1101 30-31 0.43637744488123786 1101 32-33 0.34434048312404286 1101 34-35 0.39697135952980744 1101 36-37 0.38017104960049153 1101 38-39 0.4181698292351115 1101 40-41 0.3518460622959765 1101 42-43 0.42326427974287384 1101 44-45 0.4297115134701741 1101 46-47 0.42893868955636805 1101 48-49 0.41585454695248103 1101 50-51 0.4294149108598617 1101 52-53 0.47764867116959664 1101 54-55 0.4097807206390556 1101 56-57 0.46539941871504453 1101 58-59 0.5017249669825006 1101 60-61 0.5123654911637701 1101 62-63 0.378507759783659 1101 64-65 0.3868378800247143 1101 66-67 0.3691836330868199 1101 68-69 0.40142295031667885 1101 70-71 0.4038604286796428 1101 72-73 0.32630629378937925 1101 74-75 0.3549154120618283 1101 76-77 0.4045042828039698 1101 78-79 0.291488929234788 1101 80-81 0.319861079313732 1101 82-83 0.299128096691625 1101 84-85 0.30547106469621355 1101 86-87 0.33843460110495727 1101 88-89 0.27768531962639287 1101 90-91 0.31009141762201864 1101 92-93 0.35190164577719685 1101 94-95 0.32909499032729883 1101 96-97 0.3157767944343206 1101 98-99 0.3296114648877193 1101 100 0.34646533039806826 1102 1 0.29581389830671867 1102 2 0.26623801793175517 1102 3 0.2697969809146201 1102 4 0.19513527560976485 1102 5 0.16716698109217276 1102 6 0.19025921603690676 1102 7 0.20047371303114403 1102 8 0.20284173875871403 1102 9 0.25186026890116153 1102 10-11 0.2469547138744943 1102 12-13 0.27600486192934426 1102 14-15 0.3655426103354884 1102 16-17 0.366513682073041 1102 18-19 0.3218193178520963 1102 20-21 0.3009472669484978 1102 22-23 0.36072347223579015 1102 24-25 0.2873769410327398 1102 26-27 0.3677539964828611 1102 28-29 0.39256457411386947 1102 30-31 0.3872602726661398 1102 32-33 0.35161987010103957 1102 34-35 0.34775853724888606 1102 36-37 0.3778979390536108 1102 38-39 0.4136533434766534 1102 40-41 0.4163892188812426 1102 42-43 0.3368243760348122 1102 44-45 0.31954243313102637 1102 46-47 0.31664791301934514 1102 48-49 0.2658362003348387 1102 50-51 0.21378171416306202 1102 52-53 0.19560118190169362 1102 54-55 0.21503962948871447 1102 56-57 0.2376910224503419 1102 58-59 0.17121267180041855 1102 60-61 0.19475108719888112 1102 62-63 0.17673240864135664 1102 64-65 0.2503185332721287 1102 66-67 0.17168852762408449 1102 68-69 0.19383648914335794 1102 70-71 0.18813777326871417 1102 72-73 0.23491410897677412 1102 74-75 0.21105522811382116 1102 76-77 0.20824652108122166 1102 78-79 0.19016682715380284 1102 80-81 0.23169189317407302 1102 82-83 0.21022753081050638 1102 84-85 0.23506377146924962 1102 86-87 0.19910745530460616 1102 88-89 0.23991845341569729 1102 90-91 0.21758222030591412 1102 92-93 0.25048166137434436 1102 94-95 0.23287237189600063 1102 96-97 0.172631732664847 1102 98-99 0.19243507673191118 1102 100 0.2728676799781269 1103 1 0.14717883021054945 1103 2 0.10893318506948191 1103 3 0.1681681713619625 1103 4 0.1116573184638554 1103 5 0.12331396465226874 1103 6 0.10940992543689276 1103 7 0.08314155397305001 1103 8 0.1377279211906668 1103 9 0.13936058076027535 1103 10-11 0.20360132426356614 1103 12-13 0.22725031714335842 1103 14-15 0.30433803842387164 1103 16-17 0.28434647080117514 1103 18-19 0.25705592393140364 1103 20-21 0.24597783892500757 1103 22-23 0.28081652610205765 1103 24-25 0.23294566361054336 1103 26-27 0.26253236616334164 1103 28-29 0.2986037889496558 1103 30-31 0.29370543430047746 1103 32-33 0.2734623924207753 1103 34-35 0.2516130669188854 1103 36-37 0.3042098304493521 1103 38-39 0.32514063503451496 1103 40-41 0.3556437524973717 1103 42-43 0.39056047262194227 1103 44-45 0.36670131919148474 1103 46-47 0.2988565750549057 1103 48-49 0.30025953492973656 1103 50-51 0.34443694278881054 1103 52-53 0.2751722985790721 1103 54-55 0.33947785505033323 1103 56-57 0.32976161528593906 1103 58-59 0.2995996451723002 1103 60-61 0.26502709025599813 1103 62-63 0.358658253192516 1103 64-65 0.3843976080232494 1103 66-67 0.3333547407022124 1103 68-69 0.34873829469753304 1103 70-71 0.248003919262473 1103 72-73 0.2769461943464293 1103 74-75 0.2690935128349139 1103 76-77 0.2500958937022233 1103 78-79 0.234509360480331 1103 80-81 0.2680919660961152 1103 82-83 0.3060750839680111 1103 84-85 0.15010813210263763 1103 86-87 0.18826315608097133 1103 88-89 0.13823114480138798 1103 90-91 0.18870802109080387 1103 92-93 0.1878098840774669 1103 94-95 0.23575370546915053 1103 96-97 0.22095301750155016 1103 98-99 0.15555070495802426 1103 100 0.162134215074218 1104 1 0.18603116619790683 1104 2 0.20464463607590488 1104 3 0.19600276188551646 1104 4 0.11812846997484172 1104 5 0.13014660733578154 1104 6 0.0974466354781569 1104 7 0.1261634455567915 1104 8 0.08756994878240221 1104 9 0.19004815305372347 1104 10-11 0.16926958090422772 1104 12-13 0.15855588923108854 1104 14-15 0.2420478631795362 1104 16-17 0.23357951596528181 1104 18-19 0.19720550775320334 1104 20-21 0.19732652649377513 1104 22-23 0.19810868610142052 1104 24-25 0.2076443067243261 1104 26-27 0.2162320866280396 1104 28-29 0.23870761197154167 1104 30-31 0.2373073737006166 1104 32-33 0.19777471864940566 1104 34-35 0.21826171519080617 1104 36-37 0.24606527732864691 1104 38-39 0.1594153133360834 1104 40-41 0.17340287944104205 1104 42-43 0.21874616910552902 1104 44-45 0.24964526960225442 1104 46-47 0.21948785658629077 1104 48-49 0.2012901064373196 1104 50-51 0.1758147135478012 1104 52-53 0.22001238353868757 1104 54-55 0.20822454600838824 1104 56-57 0.2224239724978858 1104 58-59 0.21515867710199643 1104 60-61 0.21493414030651792 1104 62-63 0.27547147568183306 1104 64-65 0.22263628211038622 1104 66-67 0.23380244598764932 1104 68-69 0.2602133188358735 1104 70-71 0.1560488746723081 1104 72-73 0.16508206172267137 1104 74-75 0.1622577453971985 1104 76-77 0.15167134422399897 1104 78-79 0.2390809601254844 1104 80-81 0.16762334487015806 1104 82-83 0.17916116244514058 1104 84-85 0.11442929801582835 1104 86-87 0.06569578297222023 1104 88-89 0.17123310612075926 1104 90-91 0.13183829875661957 1104 92-93 0.12937192572752565 1104 94-95 0.05706693397055318 1104 96-97 0.09300838661428656 1104 98-99 0.1256610141424943 1104 100 0.05643496209726351 1105 1 0.18065584562041437 1105 2 0.10050030382748787 1105 3 0.2080379793518361 1105 4 0.12432427885362785 1105 5 0.10965099526305977 1105 6 0.09801475581936359 1105 7 0.10372177823215623 1105 8 0.12025306176897033 1105 9 0.17133044840318945 1105 10-11 0.18166777835948977 1105 12-13 0.14828929450245454 1105 14-15 0.24711960381662124 1105 16-17 0.22121977318759178 1105 18-19 0.2108912058298884 1105 20-21 0.20497882456285765 1105 22-23 0.2340055793143847 1105 24-25 0.23201740775045465 1105 26-27 0.22923616457845242 1105 28-29 0.2857106693377318 1105 30-31 0.2940521486895804 1105 32-33 0.22974481173019257 1105 34-35 0.18835896312258882 1105 36-37 0.18019596035974672 1105 38-39 0.2357570599095311 1105 40-41 0.230985432714661 1105 42-43 0.17186564033178087 1105 44-45 0.21879606457594747 1105 46-47 0.24161717104028213 1105 48-49 0.2216936758502115 1105 50-51 0.13101092013276627 1105 52-53 0.13535196173620534 1105 54-55 0.2350114091302089 1105 56-57 0.22103890958135963 1105 58-59 0.16834705238456138 1105 60-61 0.09747202474362382 1105 62-63 1.7484097787701103E-4 1105 64-65 0.017906758860661398 1105 66-67 0.04986973874910916 1105 68-69 0.1335382374669578 1105 70-71 0.05314865359446941 1105 72-73 0.07832692005447228 1105 74-75 0.054785109628788575 1105 76-77 0.0327074857723062 1105 78-79 0.08627022293615738 1105 80-81 0.07958921963365384 1105 82-83 0.10871533675157252 1105 84-85 0.12774554606750144 1105 86-87 0.09358518410275707 1105 88-89 0.13653364477867314 1105 90-91 0.05800294180070864 1105 92-93 0.10627057060224132 1105 94-95 0.15219148764772683 1105 96-97 0.08416355489371341 1105 98-99 0.13664103086955492 1105 100 0.07553904121739663 1106 1 0.13781098658659374 1106 2 0.18251951622347917 1106 3 0.17603136097672945 1106 4 0.14605417619064553 1106 5 0.10126772012278451 1106 6 0.10349662911482937 1106 7 0.13180601051801233 1106 8 0.09534382557462351 1106 9 0.11980917753620446 1106 10-11 0.16457638919556672 1106 12-13 0.1375787311588752 1106 14-15 0.21977559596491147 1106 16-17 0.2355569802680364 1106 18-19 0.22342746792448764 1106 20-21 0.1927884637658721 1106 22-23 0.2146479178069285 1106 24-25 0.19727006986590823 1106 26-27 0.2307176991381823 1106 28-29 0.26263342890484154 1106 30-31 0.20742265609833765 1106 32-33 0.17605215795286 1106 34-35 0.161673101430992 1106 36-37 0.18029432400559386 1106 38-39 0.19026137661874998 1106 40-41 0.2645956173690678 1106 42-43 0.3149369746031496 1106 44-45 0.26807907393894936 1106 46-47 0.2743392600843535 1106 48-49 0.2523436141829407 1106 50-51 0.2248980429635523 1106 52-53 0.2719612604663695 1106 54-55 0.2621026680111669 1106 56-57 0.2983394897748681 1106 58-59 0.2871824963020799 1106 60-61 0.23163721486992017 1106 62-63 0.20377651381127038 1106 64-65 0.23182858039035636 1106 66-67 0.22414384544217114 1106 68-69 0.2461993540789038 1106 70-71 0.19074984003809448 1106 72-73 0.21225130381600366 1106 74-75 0.2834864489377651 1106 76-77 0.18040495133416812 1106 78-79 0.13117767859651508 1106 80-81 0.1273450953958175 1106 82-83 0.08921762104638731 1106 84-85 0.10713977366317096 1106 86-87 0.1278346238859669 1106 88-89 0.20335209111078 1106 90-91 0.1878288403189572 1106 92-93 0.15216643976338418 1106 94-95 0.10018751835976758 1106 96-97 0.06900491365828287 1106 98-99 0.06669362176364046 1106 100 0.08578015157576147 1107 1 0.12058940397096052 1107 2 0.08681119701148532 1107 3 0.13743477089746392 1107 4 0.056478090992243324 1107 5 0.09613932739294029 1107 6 0.07563575854531734 1107 7 0.07538626189602127 1107 8 0.0729935699048525 1107 9 0.08139031305701394 1107 10-11 0.09721719538580231 1107 12-13 0.08495604513076671 1107 14-15 0.13158900819271224 1107 16-17 0.15019325809968365 1107 18-19 0.14498453199848882 1107 20-21 0.14353296303240626 1107 22-23 0.14547145170388376 1107 24-25 0.13520571133339132 1107 26-27 0.1448055224957514 1107 28-29 0.1352457317212341 1107 30-31 0.13144222890093715 1107 32-33 0.11069097552258 1107 34-35 0.1303847057633476 1107 36-37 0.1329302413401976 1107 38-39 0.10294680763276887 1107 40-41 0.13637136242196846 1107 42-43 0.14090233436994737 1107 44-45 0.047638429383702885 1107 46-47 0.10225224293943569 1107 48-49 0.12488072000844141 1107 50-51 0.08515778584450828 1107 52-53 0.1575293810910381 1107 54-55 0.07696585433963321 1107 56-57 0.12037470299092234 1107 58-59 0.08964280448500261 1107 60-61 0.05190890797537406 1107 62-63 0.12768886257281054 1107 64-65 0.13864731457481128 1107 66-67 0.11359557878537885 1107 68-69 0.13025638661641636 1107 70-71 0.0687569137412396 1107 72-73 0.008345587527166742 1107 74-75 0.08725923544811565 1107 76-77 0.0802997456279968 1107 78-79 0.17125515182064532 1107 80-81 0.15712828978640658 1107 82-83 0.12743696471191157 1107 84-85 0.11749221978694635 1107 86-87 0.15068673011654 1107 88-89 0.16668406206806452 1107 90-91 0.13451893929305214 1107 92-93 0.19408136862969627 1107 94-95 0.1560703058035955 1107 96-97 0.14903868993202707 1107 98-99 0.09160071528570768 1107 100 0.0917489565280789 1108 1 0.09303835981003772 1108 2 0.04861099500265453 1108 3 0.07542661798039774 1108 4 0.0597698049241302 1108 5 0.0377191914277617 1108 6 -0.007980277475553521 1108 7 0.0318477939354338 1108 8 0.0037634128766086405 1108 9 0.0032502585455844724 1108 10-11 -0.008643251808742036 1108 12-13 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-0.3730887876022564 2214 68-69 -0.3555116562264615 2214 70-71 -0.39661110056275106 2214 72-73 -0.36196949462100036 2214 74-75 -0.2929531180315763 2214 76-77 -0.3014882685351097 2214 78-79 -0.3267511017424596 2214 80-81 -0.3317634671303509 2214 82-83 -0.24886821834716244 2214 84-85 -0.24735553905755125 2214 86-87 -0.23150886085108624 2214 88-89 -0.34724773678451726 2214 90-91 -0.25668713812870436 2214 92-93 -0.3076009720870161 2214 94-95 -0.28986279911252666 2214 96-97 -0.34538304634629924 2214 98-99 -0.35719746994945467 2214 100 -0.27760322186910713 2215 1 -0.2675286538999444 2215 2 -0.2541680237071233 2215 3 -0.28745512661439676 2215 4 -0.22994422335847275 2215 5 -0.23527834626007404 2215 6 -0.19783915939046182 2215 7 -0.193422889093128 2215 8 -0.17464478053447863 2215 9 -0.2425954282057745 2215 10-11 -0.2873641933484805 2215 12-13 -0.2803495812617598 2215 14-15 -0.3917420215431022 2215 16-17 -0.38220648284379166 2215 18-19 -0.4378699441779901 2215 20-21 -0.41774088249439956 2215 22-23 -0.3549512595957367 2215 24-25 -0.3896511918228711 2215 26-27 -0.43195268040243207 2215 28-29 -0.38997461766311403 2215 30-31 -0.3821143962755613 2215 32-33 -0.4146622870484933 2215 34-35 -0.4128218996696731 2215 36-37 -0.36815229930836324 2215 38-39 -0.3728322045836947 2215 40-41 -0.3504609899762272 2215 42-43 -0.4108171267314944 2215 44-45 -0.39934855979586104 2215 46-47 -0.3315982907812369 2215 48-49 -0.325518285262568 2215 50-51 -0.29204221332370395 2215 52-53 -0.3481297400807364 2215 54-55 -0.35760256077372077 2215 56-57 -0.22988441129546544 2215 58-59 -0.2753910459216371 2215 60-61 -0.2766794650262767 2215 62-63 -0.2910227378775545 2215 64-65 -0.22948328022700082 2215 66-67 -0.1502585847765232 2215 68-69 -0.2015624526523183 2215 70-71 -0.28931820063573355 2215 72-73 -0.24507878989498266 2215 74-75 -0.3056182235613676 2215 76-77 -0.28871699484851376 2215 78-79 -0.1899179777425104 2215 80-81 -0.14863032276858945 2215 82-83 -0.15874990672308797 2215 84-85 -0.1750931421331714 2215 86-87 -0.11247716287546439 2215 88-89 -0.1662728283109658 2215 90-91 -0.1533183430810965 2215 92-93 -0.20384944858365373 2215 94-95 -0.2683146833357668 2215 96-97 -0.2613325936107884 2215 98-99 -0.1824240508136512 2215 100 -0.19047258485823448 2216 1 -0.4671351293199777 2216 2 -0.4843619507877719 2216 3 -0.48637384710547593 2216 4 -0.39644962975602027 2216 5 -0.3526591080647279 2216 6 -0.3459389191405151 2216 7 -0.3357411122463887 2216 8 -0.39468147730512726 2216 9 -0.47280018850987204 2216 10-11 -0.441574974448649 2216 12-13 -0.4994173235746544 2216 14-15 -0.6214537154197401 2216 16-17 -0.5751757517745304 2216 18-19 -0.5682321576469676 2216 20-21 -0.6315681303418401 2216 22-23 -0.6282091070280558 2216 24-25 -0.6053823013120905 2216 26-27 -0.629372547048554 2216 28-29 -0.5913789438954282 2216 30-31 -0.6633007704328051 2216 32-33 -0.634688460116358 2216 34-35 -0.6168955321609317 2216 36-37 -0.6421974125650749 2216 38-39 -0.5459296274526935 2216 40-41 -0.5624450422332217 2216 42-43 -0.6947296041906483 2216 44-45 -0.6153737139216773 2216 46-47 -0.6566613865698301 2216 48-49 -0.6612411181690803 2216 50-51 -0.6999019336036838 2216 52-53 -0.6818476528466633 2216 54-55 -0.6476809995019366 2216 56-57 -0.6978714621887079 2216 58-59 -0.6899559124779913 2216 60-61 -0.6700972603600732 2216 62-63 -0.6938352593505499 2216 64-65 -0.6703888200898618 2216 66-67 -0.7327060699520302 2216 68-69 -0.7597831359377665 2216 70-71 -0.742162417025348 2216 72-73 -0.6378122350271909 2216 74-75 -0.6963741411322957 2216 76-77 -0.6113402939550525 2216 78-79 -0.6324118229239843 2216 80-81 -0.6504052690512765 2216 82-83 -0.6324031977635087 2216 84-85 -0.6215816610829634 2216 86-87 -0.6017041411923074 2216 88-89 -0.5772924451119934 2216 90-91 -0.6001371205858774 2216 92-93 -0.5996462197878287 2216 94-95 -0.5260172599715318 2216 96-97 -0.5884255259539088 2216 98-99 -0.6228629882663341 2216 100 -0.7562698698667205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 18.0 8 68.0 9 109.0 10 178.0 11 213.0 12 149.0 13 143.0 14 247.0 15 439.0 16 967.0 17 1770.0 18 3378.0 19 5465.0 20 7988.0 21 12186.0 22 18456.0 23 21878.0 24 19747.0 25 15358.0 26 12424.0 27 13184.0 28 16620.0 29 20608.0 30 24435.0 31 31371.0 32 41222.0 33 55483.0 34 84661.0 35 140952.0 36 250973.0 37 624970.0 38 803989.0 39 323866.0 40 699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.951268979088596 21.388878339240918 13.764784787833964 22.895067893836522 2 15.272018664620685 21.38852991033678 40.605738101265125 22.73371332377741 3 17.27957196790699 28.101586366930402 31.300120859713076 23.318720805449534 4 12.033795119048317 18.55184469592419 40.53918718666988 28.875172998357616 5 12.103229232799208 39.21745025690595 35.64160298033619 13.037717529958654 6 27.590232855698687 40.08153120808233 18.727081093482905 13.60115484273607 7 24.63153048256724 34.299162090568764 23.962361806802406 17.10694562006159 8 25.566563503855395 34.36746710828963 21.171906045497344 18.894063342357633 9 26.668585064295687 14.432222816011963 21.735640891624236 37.163551228068116 10-11 24.173571919356828 26.687769040585856 28.849822743974173 20.28883629608314 12-13 25.493116280344452 24.76459499298219 28.59571336007345 21.146575366599915 14-15 22.927190125807783 25.47732883775596 27.12629012291061 24.469190913525647 16-17 21.0924099968092 28.223642097474176 28.02342402656002 22.66052387915661 18-19 20.637808485190163 27.396656115479708 30.99251629169823 20.97301910763189 20-21 22.29953234163921 26.559725786591965 30.322741037853117 20.818000833915704 22-23 22.450623540885356 26.172606484292753 30.043579151696242 21.333190823125648 24-25 21.840839553444013 26.51752103875359 30.52955604755277 21.112083360249624 26-27 21.731921549282266 26.86878355972383 29.849053427373967 21.550241463619937 28-29 21.19678648821654 26.911986657348734 30.48556993048745 21.40565692394728 30-31 21.89667498234289 26.426839275674848 30.30705751910961 21.369428222872653 32-33 22.053835736521002 26.832811508364536 29.63065989553582 21.48269285957864 34-35 21.936997515841647 26.993913909916472 30.13920291599865 20.929885658243236 36-37 21.949490172249895 26.45687723260079 29.85303398799466 21.74059860715466 38-39 21.960735643398255 26.415908389301617 30.36098002524861 21.262375942051513 40-41 21.791476904013134 25.921299518142337 30.59981148530202 21.68741209254251 42-43 21.898076561304972 26.21649075063185 29.787567660578073 22.097865027485106 44-45 22.00672342669642 26.8809630869271 29.529068455195524 21.583245031180954 46-47 22.23642535265307 26.398945270749074 28.80544277878483 22.55918659781302 48-49 22.802055986621248 26.572708983773584 28.708368316570414 21.91686671303475 50-51 22.533332837418204 26.729440386531913 28.22033744022836 22.51688933582152 52-53 22.05433465293002 27.071562231384945 28.23361383327275 22.640489282412286 54-55 22.16483029239411 27.047625013580717 28.024418867558865 22.7631258264663 56-57 22.88098714641684 26.745655815413926 27.752667626005184 22.62068941216405 58-59 22.010630132202262 26.952441600537476 28.5282076710708 22.508720596189463 60-61 22.25695203690135 27.408916781495613 28.016611322345902 22.317519859257136 62-63 22.52809894964662 26.95594451152382 27.686000404042304 22.82995613478726 64-65 21.821800599389334 27.023575236595125 27.981564539674352 23.17305962434118 66-67 22.508423186858987 26.769241189838887 27.886890563270462 22.835445060031663 68-69 22.034147817790082 26.64315181954254 28.33466646777319 22.988033894894194 70-71 22.16660089202937 26.32001475231151 28.123061997293842 23.39032235836528 72-73 22.54094220715966 26.338168457782192 28.302529121940985 22.818360213117167 74-75 22.527333342468918 26.89786873339689 27.858142644321028 22.716655279813164 76-77 22.338456876319068 26.878251236382567 27.564592094081554 23.218699793216814 78-79 22.200484575090446 26.880547365874158 27.696400805497934 23.222567253537463 80-81 21.97338516922029 27.043729678198584 27.995920468715436 22.986964683865686 82-83 22.729167103037472 26.36424195881978 28.566115217944947 22.3404757201978 84-85 22.865845004734332 26.70724275789788 27.537537484630786 22.889374752736995 86-87 21.739693017228383 27.203191587239132 28.040337246472983 23.016778149059498 88-89 22.416306868811205 26.396242755104115 27.910741160091646 23.276709215993037 90-91 22.3504286052662 26.404022370865416 27.923412868532992 23.322136155335397 92-93 22.656647932926553 26.562642534007512 27.75950732416809 23.021202208897844 94-95 21.6329674674998 26.700649710380688 28.295562433076306 23.37082038904321 96-97 22.00457480451373 26.62843563022973 27.970084654437827 23.396904910818716 98-99 21.673803275466575 26.89739374465831 27.918143482051725 23.51065949782339 100 22.228368982585874 26.456635388661226 27.407269072673106 23.90772655607979 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122.0 1 92.0 2 143.5 3 208.5 4 142.0 5 128.5 6 258.0 7 659.5 8 1327.0 9 1697.0 10 1285.5 11 844.5 12 905.5 13 1091.0 14 1318.5 15 1608.0 16 1976.5 17 2324.5 18 2810.0 19 3379.0 20 3856.0 21 4483.5 22 5208.5 23 6196.0 24 7300.5 25 8487.5 26 10808.0 27 13676.5 28 16596.5 29 21970.0 30 28325.5 31 33677.5 32 41888.0 33 50334.0 34 57188.5 35 63574.0 36 72274.0 37 83359.5 38 86414.5 39 85373.0 40 83492.5 41 79480.0 42 78535.5 43 81116.0 44 85270.0 45 96395.5 46 104919.5 47 108070.0 48 111456.0 49 107674.5 50 101721.0 51 100475.0 52 99234.0 53 95443.0 54 91559.5 55 80441.0 56 70422.5 57 59068.5 58 46473.0 59 42218.5 60 34199.5 61 22881.0 62 15591.0 63 12396.0 64 8555.5 65 4333.0 66 2970.5 67 2113.5 68 1346.5 69 908.5 70 605.0 71 429.5 72 309.5 73 261.5 74 214.0 75 146.0 76 97.5 77 66.0 78 31.0 79 17.0 80 10.0 81 5.0 82 5.0 83 4.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006029249691196709 2 0.08695430885810318 3 5.481136082906099E-4 4 0.0 5 0.03703681953163693 6 0.003954248174096542 7 3.9150972020757845E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.9150972020757845E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.698116642490941E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.1745291606227352E-4 32-33 0.0014877369367887981 34-35 0.0011745291606227353 36-37 7.438684683943991E-4 38-39 6.068400663217466E-4 40-41 0.001468161450778419 42-43 5.285381222802309E-4 44-45 4.5023617823871525E-4 46-47 1.9575486010378924E-4 48-49 6.068400663217466E-4 50-51 5.676890943009888E-4 52-53 9.98349786529325E-4 54-55 0.0016639163108822083 56-57 9.591988145085672E-4 58-59 0.0023686338072558496 60-61 0.002094577003110545 62-63 0.0010570762445604618 64-65 0.004521937268397531 66-67 0.004091276576169195 68-69 0.0032886816497436585 70-71 0.0015268879088095558 72-73 0.004972173446636246 74-75 0.003915097202075784 76-77 0.0035431629678785853 78-79 7.242929823840201E-4 80-81 0.0 82-83 1.3702840207265247E-4 84-85 5.872645803113676E-5 86-87 0.0 88-89 1.1745291606227352E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 2.936322901556838E-4 98-99 5.481136082906099E-4 100 9.396233284981882E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2554215.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.71014406619903 #Duplication Level Percentage of deduplicated Percentage of total 1 66.59619616226304 32.439103093246835 2 16.37637327796755 15.953910033033026 3 6.879683990607996 10.053311949373157 4 3.4619768929056023 6.745335728291359 5 1.9760359596189714 4.812649813651496 6 1.2481061486668257 3.647725818688194 7 0.8286067708497977 2.8253088627625127 8 0.549539822599904 2.141453114316382 9 0.38887547563206565 1.7047962397664627 >10 1.6024133700051493 12.583739273314599 >50 0.05805612122596432 1.9692195790770715 >100 0.03130268685060831 2.7698909836488745 >500 0.0018888805376542433 0.6138224190537225 >1k 6.746001920193727E-4 0.6543057820234326 >5k 2.6984007680774905E-4 1.0854273097528135 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8615 0.33728562395882883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7901 0.3093318299360077 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4037 0.15805247404779943 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.830194404151569E-5 0.0 22-23 0.0 0.0 0.0 4.698116642490941E-4 0.0 24-25 0.0 0.0 0.0 0.001252831104664251 0.0 26-27 0.0 0.0 0.0 0.0024469357512973654 0.0 28-29 0.0 0.0 0.0 0.006401183925393908 0.0 30-31 0.0 0.0 0.0 0.017657088381361787 0.0 32-33 0.0 0.0 0.0 0.04183281360417976 0.0 34-35 0.0 0.0 0.0 0.08229534318763299 0.0 36-37 0.0 0.0 0.0 0.14536755911307386 0.0 38-39 0.0 0.0 0.0 0.23022729096806652 0.0 40-41 0.0 0.0 0.0 0.3168488165639932 0.0 42-43 0.0 0.0 0.0 0.40394015382416903 0.0 44-45 0.0 0.0 0.0 0.5114682984791805 0.0 46-47 0.0 0.0 0.0 0.6353615494388687 0.0 48-49 0.0 0.0 0.0 0.7652644746037431 0.0 50-51 0.0 0.0 0.0 0.8974577316318322 0.0 52-53 0.0 0.0 0.0 1.0283981575552568 0.0 54-55 0.0 0.0 0.0 1.171260054459002 0.0 56-57 0.0 0.0 0.0 1.330369604751362 0.0 58-59 0.0 0.0 0.0 1.5088784616800073 0.0 60-61 0.0 0.0 0.0 1.6921833126811956 0.0 62-63 0.0 0.0 0.0 1.875194531392228 0.0 64-65 0.0 0.0 0.0 2.0633541029239906 0.0 66-67 0.0 0.0 0.0 2.2690728854070623 0.0 68-69 0.0 0.0 0.0 2.496911967081863 0.0 70-71 0.0 0.0 0.0 2.7372206333452747 0.0 72-73 0.0 0.0 0.0 2.9800153863320045 0.0 74-75 0.0 0.0 0.0 3.2218117895322047 0.0 76-77 0.0 0.0 0.0 3.4812652811137665 0.0 78-79 0.0 0.0 0.0 3.7632697325792854 0.0 80-81 0.0 0.0 0.0 4.064967122971245 0.0 82-83 0.0 0.0 0.0 4.371127724173572 0.0 84-85 0.0 0.0 0.0 4.675918041355171 0.0 86-87 0.0 0.0 0.0 4.998306720460102 0.0 88 0.0 0.0 0.0 5.248931667850983 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5795 0.0 55.859905 1 GTATCAA 10615 0.0 43.08355 1 CAACGCA 12625 0.0 35.193935 5 ATCAACG 12685 0.0 35.027466 3 TATCAAC 13580 0.0 33.397076 2 AACGCAG 13915 0.0 32.49168 6 TCAACGC 14650 0.0 30.618092 4 GTGGTAT 1305 0.0 30.285446 1 ACGCAGA 14970 0.0 29.635588 7 CGCAGAG 15145 0.0 29.324179 8 GCAGAGT 16525 0.0 26.590925 9 TGATCGC 1235 0.0 26.25702 9 GTACTAG 1635 0.0 25.611643 1 CGTTAAC 530 0.0 24.85692 1 GTATAAG 1515 0.0 24.845203 1 GAGTACT 10325 0.0 24.556398 12-13 TACATGG 10870 0.0 24.367445 2 GTACATG 11105 0.0 23.980768 1 ACATGGG 10905 0.0 23.886444 3 GTGATCG 1340 0.0 23.848848 8 >>END_MODULE