FastQCFastQC Report
Mon 16 Jan 2017
ERR1146549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146059
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.035.7459141
CAACGCA31100.029.9189625
GTATCAA35100.028.9314771
ATCAACG32950.028.667013
TAACGCC6700.026.6532754
CTAACGC6850.026.0696243
GTACATG62150.025.8707471
ACGCAGA37150.024.79017
TACATGG63200.024.6866842
TATCAAC40000.024.6718122
GTACTAG12650.024.528861
CGCCTAC7100.024.486377
TCTAACG7300.024.4625952
ACATGGG63250.024.2956773
AACGCAG40200.024.1972396
CGCAGAG38750.023.7698338
GGCGTCG8500.023.220538
ATACGGG8700.022.6867263
CATGGGG49550.022.5722624
CTGTGCG15100.022.0964839
Produced by FastQC (version 0.11.5)