##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146549_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2146059
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02429569736899	31.0	31.0	34.0	28.0	34.0
2	31.203196184261476	31.0	31.0	34.0	28.0	34.0
3	31.285757754097162	31.0	31.0	34.0	28.0	34.0
4	34.93174558574578	37.0	35.0	37.0	32.0	37.0
5	34.936500347846916	37.0	35.0	37.0	32.0	37.0
6	35.161559397947585	37.0	35.0	37.0	32.0	37.0
7	35.14639718665703	37.0	35.0	37.0	32.0	37.0
8	35.06416598984464	37.0	35.0	37.0	32.0	37.0
9	36.711979493574034	39.0	37.0	39.0	32.0	39.0
10-11	36.68015371432006	39.0	37.0	39.0	32.0	39.0
12-13	36.62877232173021	39.0	37.0	39.0	32.0	39.0
14-15	38.00840564029228	40.0	38.0	41.0	33.0	41.0
16-17	38.01585907004421	40.0	38.0	41.0	33.0	41.0
18-19	38.04685332509497	40.0	38.0	41.0	33.0	41.0
20-21	38.1352637555631	40.0	38.0	41.0	33.0	41.0
22-23	38.15843320244224	40.0	38.0	41.0	33.0	41.0
24-25	38.21545866166774	40.0	38.0	41.0	33.0	41.0
26-27	38.24667867938393	40.0	38.0	41.0	33.0	41.0
28-29	38.21045367345446	40.0	38.0	41.0	33.0	41.0
30-31	38.1974927063981	40.0	38.0	41.0	33.0	41.0
32-33	38.207895495883385	40.0	38.0	41.0	33.0	41.0
34-35	38.1531330219719	40.0	38.0	41.0	32.5	41.0
36-37	38.058506546185356	40.0	38.0	41.0	32.0	41.0
38-39	38.01054863822476	40.0	38.0	41.0	32.0	41.0
40-41	37.94073275711432	40.0	38.0	41.0	32.0	41.0
42-43	37.81095743406868	40.0	38.0	41.0	32.0	41.0
44-45	37.654354330426145	40.0	37.0	41.0	31.5	41.0
46-47	37.540187618327366	40.0	37.0	41.0	31.0	41.0
48-49	37.40835410396453	40.0	36.5	41.0	31.0	41.0
50-51	37.26817529247798	40.0	36.0	41.0	31.0	41.0
52-53	37.12675187401651	40.0	36.0	41.0	30.5	41.0
54-55	36.939550124204416	39.0	35.0	41.0	30.0	41.0
56-57	36.7802660597868	39.0	35.0	41.0	30.0	41.0
58-59	36.5427581441144	39.0	35.0	41.0	30.0	41.0
60-61	36.36368781100613	38.5	35.0	40.5	30.0	41.0
62-63	36.123141302266156	38.0	35.0	40.0	30.0	41.0
64-65	35.87224838646095	37.0	35.0	40.0	30.0	41.0
66-67	35.615922488617514	37.0	35.0	40.0	29.5	41.0
68-69	35.32228680572156	36.0	34.5	39.5	29.0	41.0
70-71	35.084565708584904	36.0	34.0	39.0	29.0	41.0
72-73	34.781591512628495	35.0	34.0	39.0	29.0	41.0
74-75	34.47345110269569	35.0	34.0	38.0	29.0	40.0
76-77	34.17973806871106	35.0	34.0	37.0	28.5	39.5
78-79	33.90006355836442	35.0	34.0	37.0	28.0	39.0
80-81	33.63976549572961	35.0	34.0	36.0	28.0	39.0
82-83	33.3806565895905	35.0	34.0	36.0	28.0	37.0
84-85	33.16141890786787	35.0	33.0	35.5	27.0	37.0
86-87	32.96860897114199	35.0	33.0	35.0	27.0	37.0
88-89	32.83204306125786	35.0	33.0	35.0	27.0	36.0
90-91	32.65563877787144	35.0	33.0	35.0	26.5	36.0
92-93	32.54719255155614	35.0	33.0	35.0	26.5	36.0
94-95	32.45133078820294	35.0	33.0	35.0	26.0	36.0
96-97	32.36355151466013	35.0	33.0	35.0	26.0	35.0
98-99	32.28490829003303	35.0	33.0	35.0	25.5	35.0
100	31.309272485052833	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35781812523194745
1101	2	0.3241858910344746
1101	3	0.270223237156781
1101	4	0.43214266222620523
1101	5	0.422243789496747
1101	6	0.41863497155343055
1101	7	0.3807999136659461
1101	8	0.4512260102371215
1101	9	0.5893582654007616
1101	10-11	0.5712318262251301
1101	12-13	0.48544391621969396
1101	14-15	0.6040320078899342
1101	16-17	0.6277559150035614
1101	18-19	0.5624182487403928
1101	20-21	0.4845518376569302
1101	22-23	0.5595326053815128
1101	24-25	0.5658983436506375
1101	26-27	0.6118549186271736
1101	28-29	0.6897539442899117
1101	30-31	0.5968237102622282
1101	32-33	0.6653614160641368
1101	34-35	0.6146872344817069
1101	36-37	0.6454408512730865
1101	38-39	0.636623811393882
1101	40-41	0.6471069300697323
1101	42-43	0.5702992006447971
1101	44-45	0.5533630517847214
1101	46-47	0.526417991812572
1101	48-49	0.48464213046030835
1101	50-51	0.49294517301145646
1101	52-53	0.42795379320340743
1101	54-55	0.4920263577596202
1101	56-57	0.4946157849774764
1101	58-59	0.48979352432678525
1101	60-61	0.3719078814499852
1101	62-63	0.40068502240646353
1101	64-65	0.36219418606535925
1101	66-67	0.36523338716084197
1101	68-69	0.4349468023918348
1101	70-71	0.3598830581746526
1101	72-73	0.40045889087539877
1101	74-75	0.3577980961040481
1101	76-77	0.31665253120906556
1101	78-79	0.38088228989625605
1101	80-81	0.31873976238327373
1101	82-83	0.32523364378320707
1101	84-85	0.4223694470070214
1101	86-87	0.3734898486299798
1101	88-89	0.34864554282552973
1101	90-91	0.4541692036838185
1101	92-93	0.3765598571166322
1101	94-95	0.3249222635901319
1101	96-97	0.23365982425957554
1101	98-99	0.3043045846418835
1101	100	0.17050189821837236
1102	1	0.1230176134144969
1102	2	0.20778739242872746
1102	3	0.23346958832883047
1102	4	0.27634912049202853
1102	5	0.20709408762331805
1102	6	0.3472537030313845
1102	7	0.32304424736903314
1102	8	0.39576784611022475
1102	9	0.4878303815199345
1102	10-11	0.46211253539030395
1102	12-13	0.5231657372869876
1102	14-15	0.6023105368641524
1102	16-17	0.47938289847355975
1102	18-19	0.5617752839834296
1102	20-21	0.5077684589522278
1102	22-23	0.4795471746767177
1102	24-25	0.5211409247810295
1102	26-27	0.5132790908854332
1102	28-29	0.5282996637316373
1102	30-31	0.450169681355419
1102	32-33	0.4514437183912037
1102	34-35	0.4635556307003057
1102	36-37	0.43840241529638746
1102	38-39	0.4597241857963894
1102	40-41	0.4787612073167935
1102	42-43	0.4384451868749224
1102	44-45	0.4810283931816244
1102	46-47	0.4872348657053962
1102	48-49	0.4299904808896997
1102	50-51	0.3905136853586981
1102	52-53	0.39897343100504656
1102	54-55	0.38150240711546957
1102	56-57	0.334096328109446
1102	58-59	0.3401077338833929
1102	60-61	0.38790690701010533
1102	62-63	0.4181252492712062
1102	64-65	0.3293982531692592
1102	66-67	0.24948258360271325
1102	68-69	0.318039324511858
1102	70-71	0.3208585150615946
1102	72-73	0.37478381927063253
1102	74-75	0.3344324706531907
1102	76-77	0.2896756836166716
1102	78-79	0.32274808208985917
1102	80-81	0.27005218152486776
1102	82-83	0.24886747671407505
1102	84-85	0.28190453039022856
1102	86-87	0.34378941793701046
1102	88-89	0.2894782395405855
1102	90-91	0.31795256110221004
1102	92-93	0.3868252973766033
1102	94-95	0.31768314503248973
1102	96-97	0.39507756332254473
1102	98-99	0.3523356011576908
1102	100	0.2529350178214038
1103	1	-0.0012350831220686587
1103	2	0.04491648881268162
1103	3	0.05184062881646767
1103	4	0.025832320424036936
1103	5	0.1041241521274685
1103	6	0.07152831326786213
1103	7	0.1418659076012716
1103	8	0.14448347299992292
1103	9	0.2394757490041357
1103	10-11	0.2025767520752524
1103	12-13	0.25517863764233084
1103	14-15	0.2967002498184286
1103	16-17	0.24759916603628085
1103	18-19	0.23179799540849189
1103	20-21	0.2842049755808844
1103	22-23	0.216448693844562
1103	24-25	0.2255029737665808
1103	26-27	0.2347299677539496
1103	28-29	0.26328996638523705
1103	30-31	0.18437056849226963
1103	32-33	0.18917519223353452
1103	34-35	0.24352558305285044
1103	36-37	0.2682915623643751
1103	38-39	0.21443527514944094
1103	40-41	0.27970385254016605
1103	42-43	0.25075203233015486
1103	44-45	0.23177341149487773
1103	46-47	0.23990018177905625
1103	48-49	0.20539796768272112
1103	50-51	0.13088234121793363
1103	52-53	0.17660071861843818
1103	54-55	0.21554459205617604
1103	56-57	0.17312085269730915
1103	58-59	0.2661412806002872
1103	60-61	0.14676272459885809
1103	62-63	0.12956627803275467
1103	64-65	0.18188533939979123
1103	66-67	0.17404099168518883
1103	68-69	0.1418163180709371
1103	70-71	0.1282395857559564
1103	72-73	0.07228145517889573
1103	74-75	0.08267889982410281
1103	76-77	0.11702956043033197
1103	78-79	0.13105217539331449
1103	80-81	0.13141158590907764
1103	82-83	0.20311776108172808
1103	84-85	0.22614490729636572
1103	86-87	0.1578231246737829
1103	88-89	0.12090306688412511
1103	90-91	0.21434604805619983
1103	92-93	0.28551354994778677
1103	94-95	0.19962314612936183
1103	96-97	0.14449025004898886
1103	98-99	0.0791258352924018
1103	100	0.050821141131194736
1104	1	0.13799762706700847
1104	2	0.04664415577401115
1104	3	0.040221879541530114
1104	4	0.13408040130624244
1104	5	0.0791061923388412
1104	6	0.07414466481223769
1104	7	0.1277775084508832
1104	8	0.10749140462434781
1104	9	0.16603249592382952
1104	10-11	0.19277929085012602
1104	12-13	0.1800164784731919
1104	14-15	0.3015281204328275
1104	16-17	0.23264435667801564
1104	18-19	0.19829830856674135
1104	20-21	0.25536687455782925
1104	22-23	0.24782853584422782
1104	24-25	0.25184542859970094
1104	26-27	0.28582641098859085
1104	28-29	0.2508377890106672
1104	30-31	0.33549292787654394
1104	32-33	0.3030870340357694
1104	34-35	0.26254978905726034
1104	36-37	0.2301973418718788
1104	38-39	0.24056391888507278
1104	40-41	0.24298394381814603
1104	42-43	0.1471840897025345
1104	44-45	0.2025999351108041
1104	46-47	0.18105105350877437
1104	48-49	0.1939303864038706
1104	50-51	0.21250521440160952
1104	52-53	0.168768670384857
1104	54-55	0.22756313487227686
1104	56-57	0.14894840299587742
1104	58-59	0.1772753990223208
1104	60-61	0.15001793270851493
1104	62-63	0.08290106120459484
1104	64-65	0.09642405884882521
1104	66-67	0.0768251397052282
1104	68-69	0.13507691669391164
1104	70-71	0.07393523167103666
1104	72-73	0.12373514732550461
1104	74-75	0.11658021254636708
1104	76-77	0.11595649670054087
1104	78-79	0.17923101904288785
1104	80-81	0.13447720415697972
1104	82-83	0.11947404664127248
1104	84-85	0.09813464573793595
1104	86-87	0.15712271295858216
1104	88-89	0.0920407446253364
1104	90-91	0.08221445448326392
1104	92-93	0.031386806806146694
1104	94-95	0.047497294649851085
1104	96-97	0.0013319818524593074
1104	98-99	-0.039986529138829496
1104	100	-0.12251106073259166
1105	1	0.09287575065329534
1105	2	0.16396218458883283
1105	3	0.15376936878772796
1105	4	0.1681686093127439
1105	5	0.21991485518704934
1105	6	0.1521308094690781
1105	7	0.20915752209919702
1105	8	0.30772775822313747
1105	9	0.2834569126115838
1105	10-11	0.2813927214085581
1105	12-13	0.31608608872937083
1105	14-15	0.43827487512347574
1105	16-17	0.4475258156678876
1105	18-19	0.3866129904765785
1105	20-21	0.4132138907134504
1105	22-23	0.3906973854750291
1105	24-25	0.34833443710306256
1105	26-27	0.3674340563470082
1105	28-29	0.42418245121479714
1105	30-31	0.3478142729259588
1105	32-33	0.36743061127528165
1105	34-35	0.3108589886370794
1105	36-37	0.37094993543571064
1105	38-39	0.38070837636442434
1105	40-41	0.34628513076177114
1105	42-43	0.3011723889534039
1105	44-45	0.33969891992130385
1105	46-47	0.2803310663903389
1105	48-49	0.24842685547663024
1105	50-51	0.17109445369955978
1105	52-53	0.22947109871201832
1105	54-55	0.2860170411282681
1105	56-57	0.26610349542873735
1105	58-59	0.23548681760397017
1105	60-61	0.2016658904447297
1105	62-63	0.17542304803166786
1105	64-65	0.26589658585792364
1105	66-67	0.2343970809101137
1105	68-69	0.15518901692470877
1105	70-71	0.17739600042405357
1105	72-73	0.15424690174778277
1105	74-75	0.1831751872049665
1105	76-77	0.2356299146841181
1105	78-79	0.21852456522864117
1105	80-81	0.17402138483617335
1105	82-83	0.143232088030949
1105	84-85	0.18996319449234278
1105	86-87	0.18964050177769565
1105	88-89	0.12056946743413732
1105	90-91	0.14990206135036743
1105	92-93	0.13952960041750373
1105	94-95	0.15756992593824037
1105	96-97	0.12659076316499807
1105	98-99	0.1732751505244181
1105	100	0.0962578686411959
1106	1	0.08573308214788611
1106	2	0.08142298543260651
1106	3	0.15316422575279276
1106	4	0.14318360893723536
1106	5	0.1556955206504398
1106	6	0.1559868683682879
1106	7	0.1451427560314471
1106	8	0.24074824519306048
1106	9	0.29782013625577974
1106	10-11	0.23113624227227092
1106	12-13	0.1507855329798744
1106	14-15	0.24006927999531769
1106	16-17	0.27516971096514453
1106	18-19	0.226415401888012
1106	20-21	0.282611314326779
1106	22-23	0.2604268331009649
1106	24-25	0.3242120698775963
1106	26-27	0.19720374092987214
1106	28-29	0.24254973899383714
1106	30-31	0.2926971004810355
1106	32-33	0.15868545881554752
1106	34-35	0.20457803514788253
1106	36-37	0.26365418616835967
1106	38-39	0.25392214185200146
1106	40-41	0.2525650732721516
1106	42-43	0.2256456689895856
1106	44-45	0.19248312463584938
1106	46-47	0.15882502981518343
1106	48-49	0.18447635877654278
1106	50-51	0.2681350092179784
1106	52-53	0.20127820065252422
1106	54-55	0.2602337801315002
1106	56-57	0.1219414235576366
1106	58-59	0.25687778828392993
1106	60-61	0.2636354876295144
1106	62-63	0.25445693604162756
1106	64-65	0.19137480978685062
1106	66-67	0.15419829771596483
1106	68-69	0.18409352027826742
1106	70-71	0.16754762248798727
1106	72-73	0.25778892793090336
1106	74-75	0.172508122749079
1106	76-77	0.2288996326911814
1106	78-79	0.132729704797363
1106	80-81	0.11432045506577992
1106	82-83	0.1533385750487497
1106	84-85	0.20744358562972565
1106	86-87	0.1362721163645162
1106	88-89	0.1582797510281324
1106	90-91	0.2769286612346633
1106	92-93	0.22409560578023502
1106	94-95	0.24938480416275155
1106	96-97	0.15379362411125896
1106	98-99	0.18852102057277875
1106	100	0.13340009561708044
1107	1	0.06296797883854666
1107	2	0.09316433127935042
1107	3	0.12825038647804732
1107	4	0.1775214218748573
1107	5	0.12431139663009816
1107	6	0.11536853556727067
1107	7	0.24205197648202414
1107	8	0.21716266182082933
1107	9	0.24698722467677925
1107	10-11	0.3152839955091338
1107	12-13	0.20351791142863362
1107	14-15	0.22607193336239106
1107	16-17	0.30732684925961706
1107	18-19	0.24524497218995833
1107	20-21	0.17280363430319312
1107	22-23	0.20941762002085085
1107	24-25	0.20648232022769264
1107	26-27	0.15190762025081028
1107	28-29	0.0727840214791513
1107	30-31	0.13479389924279417
1107	32-33	0.11898239024566237
1107	34-35	0.10665316474521092
1107	36-37	0.15161173229517289
1107	38-39	0.10382902832228069
1107	40-41	0.14224666922331153
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2216	62-63	-0.4847744121513031
2216	64-65	-0.5474074034240601
2216	66-67	-0.5905328685385882
2216	68-69	-0.6142993302491462
2216	70-71	-0.5853958393789185
2216	72-73	-0.5571756730114501
2216	74-75	-0.5554945514414129
2216	76-77	-0.5841985704865564
2216	78-79	-0.5073397492069631
2216	80-81	-0.4507870057939414
2216	82-83	-0.49584905916449884
2216	84-85	-0.5090440102622367
2216	86-87	-0.47753194613604677
2216	88-89	-0.5400546037211669
2216	90-91	-0.4924474770566434
2216	92-93	-0.5412831071124415
2216	94-95	-0.4839791274448473
2216	96-97	-0.588240935540103
2216	98-99	-0.5101232668315134
2216	100	-0.5404096667353713
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	55.0
1	27.0
2	20.0
3	28.0
4	22.0
5	35.0
6	121.0
7	749.0
8	2184.0
9	2432.0
10	1977.0
11	2118.0
12	2500.0
13	3394.0
14	4385.0
15	4721.0
16	5077.0
17	5523.0
18	6353.0
19	6993.0
20	7751.0
21	8761.0
22	10120.0
23	11871.0
24	15309.0
25	19513.0
26	21327.0
27	19972.0
28	21095.0
29	25309.0
30	29399.0
31	32759.0
32	41225.0
33	55446.0
34	84858.0
35	133723.0
36	264691.0
37	536386.0
38	584379.0
39	173248.0
40	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.465577696376485	21.051721115181177	13.263945360001792	24.218755828440553
2	15.360791256437418	21.25965758139071	39.63812991849861	23.741421243673262
3	17.8445284847807	26.5831544951871	30.70484365934304	24.867473360689164
4	12.492945662846724	18.196951035094365	39.96564520330704	29.344458098751865
5	13.304862237988408	38.37744582212152	35.58977016518766	12.72792177470242
6	29.14590709186377	38.8793534523536	18.829460568730404	13.145278887052223
7	25.560625462489213	33.13897136807338	23.87795690206319	17.422446267374216
8	25.086814778363685	34.746321905283814	21.564129233327183	18.60273408302531
9	25.93778309641775	15.465499051219247	21.656107053784126	36.940610798578874
10-11	24.036742265908202	26.63886508403618	29.110694196105076	20.213698453950542
12-13	24.674757914946362	24.837460506817525	28.22287668807143	22.264904890164683
14-15	22.372137229823863	26.138251798649996	25.86700673464143	25.622604236884715
16-17	22.019897794177272	28.576062065385255	26.924910502333322	22.47912963810415
18-19	21.727915940282482	28.753256787468956	28.119793858343083	21.399033413905475
20-21	23.075725813573158	27.943708978831683	27.181122125037778	21.799443082557385
22-23	23.34097469404074	27.647552081282516	26.79643233341713	22.21504089125961
24-25	22.85366858940769	27.781157479689362	27.275538023044465	22.08963590785848
26-27	22.701613542085088	27.944530238652877	26.838522769741495	22.51533344952054
28-29	22.292279793385955	28.253601521202775	27.080825197984932	22.373293487426334
30-31	22.668552448701178	27.932936682227478	27.056187838802913	22.34232303026843
32-33	22.80773823740148	28.264232496635927	26.3908613769682	22.537167888994393
34-35	22.9980699600454	28.036236191406466	26.930593358546805	22.03510049000133
36-37	22.78894975302603	27.679283618205304	26.91190147233628	22.619865156432382
38-39	22.75730898283492	27.65725955758694	27.240582113373268	22.34484934620487
40-41	22.775284899621003	27.208496375021674	27.458465149314804	22.55775357604252
42-43	22.568205191305925	27.533296559194365	27.061408384270642	22.837089865229075
44-45	22.52488936441842	27.89219203759408	27.224195265829525	22.358723332157975
46-47	22.589480317779348	27.656139588736394	26.86949866411111	22.88488142937315
48-49	22.80438762394525	27.39848113538451	27.103727709082776	22.693403531587467
50-51	22.775174593334167	27.355492188310258	26.983663447677937	22.88566977067764
52-53	22.387919518145523	27.668620634685354	26.910269425714255	23.033190421454865
54-55	22.437505652064065	27.546062574059523	26.937667405979955	23.07876436789646
56-57	22.923352247194153	27.36483084743171	26.716162493869934	22.9956544115042
58-59	22.376224746986235	27.360774254057475	27.36219600706495	22.900804991891345
60-61	22.481879489339327	27.617014750461603	27.01859155769965	22.882514202499415
62-63	22.79628875619168	27.20919993930971	26.79273497152054	23.201776332978064
64-65	22.171822206083444	27.413628467872396	26.936325139614542	23.478224186429614
66-67	22.741204302578982	27.12307566805347	26.813481103380767	23.322238925986785
68-69	22.327578872781476	27.09128393268994	27.082659817098826	23.498477377429758
70-71	22.27510090147038	26.9700843106488	27.02478880382927	23.730025984051554
72-73	22.618395295116837	26.999611469096646	27.154860680089897	23.227132555696617
74-75	22.577496342994912	27.338641934217726	27.04505598084652	23.038805741940838
76-77	22.414829493502157	27.11455257441982	26.96792367559096	23.502694256487064
78-79	22.436952990673092	27.290618854867127	26.83549590734296	23.43693224711682
80-81	22.388366541219316	27.268389738024347	27.18958306819702	23.153660652559317
82-83	22.678883669501356	26.572680398674812	27.851111804128927	22.897324127694905
84-85	22.846515621500338	27.110285221646517	26.954292798221598	23.088906358631544
86-87	22.114657632453063	27.382481730049417	27.32351633271587	23.17934430478165
88-89	22.649547750170715	26.626479629530365	27.209195552313453	23.514777067985467
90-91	22.55122502841044	26.718820064717075	27.24682544195833	23.483129464914153
92-93	22.893719492625788	26.592862798128262	27.370966174353423	23.142451534892526
94-95	22.010702989704896	26.73013409037407	27.679022186794207	23.580140733126825
96-97	22.287972762961505	26.680010478652544	27.446378478861373	23.58563827952458
98-99	22.06451633953721	27.009143838920767	27.192677843868147	23.733661977673876
100	22.497373506487694	26.55247440392347	26.945073425292453	24.005078664296377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	484.0
1	355.5
2	226.5
3	227.5
4	217.0
5	191.0
6	171.0
7	179.5
8	197.5
9	201.5
10	171.5
11	146.0
12	159.0
13	173.5
14	187.0
15	216.0
16	263.5
17	342.5
18	445.5
19	595.5
20	821.5
21	1120.5
22	1612.0
23	2282.5
24	3169.5
25	4733.5
26	7110.0
27	9997.5
28	12856.0
29	17050.0
30	22360.0
31	27492.5
32	34488.5
33	40646.5
34	45745.5
35	50878.5
36	56372.5
37	63837.5
38	66913.0
39	67373.5
40	67772.0
41	67499.0
42	68567.0
43	71903.0
44	76707.5
45	84430.0
46	90593.0
47	93441.5
48	94675.5
49	91334.5
50	86209.0
51	85011.0
52	84515.5
53	81798.5
54	77765.0
55	69015.0
56	61845.5
57	52148.0
58	42015.5
59	37913.0
60	31099.0
61	23458.5
62	18152.5
63	13994.0
64	10012.5
65	6069.0
66	4149.0
67	2979.5
68	2115.0
69	1583.5
70	1227.5
71	913.5
72	778.0
73	736.5
74	582.0
75	382.0
76	251.0
77	176.5
78	112.0
79	69.5
80	45.0
81	36.5
82	32.0
83	27.5
84	22.0
85	19.5
86	16.0
87	14.0
88	11.5
89	10.0
90	9.0
91	5.5
92	3.0
93	6.5
94	5.5
95	3.0
96	3.5
97	3.0
98	4.5
99	4.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06537564903853996
2	0.009598990521695817
3	0.01230161892100823
4	0.01006496093537037
5	0.011975439631436042
6	0.01220842483827332
7	0.002935613606149691
8	0.005172271591787551
9	0.0068497650810159455
10-11	0.006430391708708847
12-13	0.005545047922727195
14-15	0.009948468331951732
16-17	0.008993228983918895
18-19	0.007269138453323045
20-21	0.00650028727076003
22-23	0.0029123150854659633
24-25	0.003611270705977794
26-27	0.004519913012643175
28-29	0.005847928691615654
30-31	0.00822437780135588
32-33	0.010321244662891374
34-35	0.011579364779812671
36-37	0.018056353529888974
38-39	0.018382532819461162
40-41	0.018219443174675068
42-43	0.024673133404067644
44-45	0.02863388192030135
46-47	0.03126661475756258
48-49	0.03292080972610725
50-51	0.03338678013978181
52-53	0.031732585171237136
54-55	0.03844255912815072
56-57	0.0417043520238726
58-59	0.03820957392131344
60-61	0.03904832066592764
62-63	0.034505109132600735
64-65	0.036229199663196585
66-67	0.038861932500457816
68-69	0.042473203206435606
70-71	0.041797546106607505
72-73	0.03720773753191315
74-75	0.04012005261737911
76-77	0.03949099255891846
78-79	0.037953290193792434
80-81	0.04361483071993827
82-83	0.039700679245072014
84-85	0.035530244042684754
86-87	0.034155631322344816
88-89	0.03140640588166495
90-91	0.03359646682593535
92-93	0.03627579670456404
94-95	0.04025984374148148
96-97	0.034714795818754286
98-99	0.03168598812986968
100	0.02856398635825017
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2146059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.349197554689034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.8954127406344	39.38550420688537
2	15.24785849526982	17.18409181271805
3	6.243484278544561	10.554459871239079
4	3.154574297094687	7.110309210717312
5	1.8075676030171948	5.092749197793581
6	1.066793525055045	3.6067775456033893
7	0.6923886861880959	2.731088280385123
8	0.4709772407787211	2.1231351667522
9	0.30820421232374046	1.5630354042676
>10	1.0917938232212805	9.046075978024373
>50	0.011089148643855866	0.4173186095370565
>100	0.009593129169268006	1.1020020596860833
>500	2.628200593956085E-4	0.08345265639070981
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.319408273491083E-5
2	0.0	0.0	0.0	0.0	9.319408273491083E-5
3	0.0	0.0	0.0	0.0	9.319408273491083E-5
4	0.0	0.0	0.0	0.0	9.319408273491083E-5
5	0.0	0.0	0.0	0.0	9.319408273491083E-5
6	0.0	0.0	0.0	0.0	1.8638816546982166E-4
7	0.0	0.0	0.0	0.0	1.8638816546982166E-4
8	0.0	0.0	0.0	4.6597041367455415E-5	1.8638816546982166E-4
9	0.0	0.0	0.0	4.6597041367455415E-5	1.8638816546982166E-4
10-11	0.0	0.0	0.0	4.6597041367455415E-5	1.8638816546982166E-4
12-13	0.0	0.0	0.0	4.6597041367455415E-5	1.8638816546982166E-4
14-15	0.0	0.0	0.0	4.6597041367455415E-5	4.892689343582819E-4
16-17	0.0	0.0	0.0	6.989556205118312E-5	6.523585791443758E-4
18-19	0.0	0.0	0.0	9.319408273491083E-5	6.989556205118312E-4
20-21	0.0	0.0	0.0	1.6308964478609395E-4	6.989556205118312E-4
22-23	0.0	0.0	0.0	6.989556205118312E-4	7.222541411955589E-4
24-25	0.0	0.0	0.0	0.0017007920099121226	7.688511825630143E-4
26-27	0.0	0.0	0.0	0.0032151958543544237	8.620452652979252E-4
28-29	0.0	0.0	0.0	0.009272811232123628	8.853437859816528E-4
30-31	0.0	0.0	0.0	0.026117641686458762	8.853437859816528E-4
32-33	0.0	0.0	0.0	0.0639078422354651	9.086423066653806E-4
34-35	0.0	0.0	0.0	0.12541593684050625	9.785378687165638E-4
36-37	0.0	0.0	0.0	0.22066028939558513	9.785378687165638E-4
38-39	0.0	0.0	0.0	0.3440259564159234	0.0011416275135026576
40-41	0.0	0.0	0.0	0.46818377313950826	0.0012115230755538407
42-43	0.0	0.0	0.0	0.5960926516931734	0.0012348215962375685
44-45	0.0	0.0	0.0	0.7520995461914142	0.0013746127203399347
46-47	0.0	0.0	0.0	0.9358549788239745	0.0015610008858097564
48-49	0.0	0.0	0.0	1.1255748327515693	0.0017240905305958504
50-51	0.0	0.0	0.0	1.3100292210046416	0.0017240905305958504
52-53	0.0	0.0	0.0	1.4898938006830194	0.0018871801753819442
54-55	0.0	0.0	0.0	1.6830851341924897	0.002050269820168038
56-57	0.0	0.0	0.0	1.9086614114523412	0.0020968668615354933
58-59	0.0	0.0	0.0	2.152270743721398	0.002166762423586677
60-61	0.0	0.0	0.0	2.4006096756892514	0.0021900609442704047
62-63	0.0	0.0	0.0	2.6467352481921513	0.0021900609442704047
64-65	0.0	0.0	0.0	2.9007823177275185	0.0022133594649541323
66-67	0.0	0.0	0.0	3.1838127469934423	0.0022599565063215876
68-69	0.0	0.0	0.0	3.485947963220023	0.002283255027005315
70-71	0.0	0.0	0.0	3.800081917598724	0.002329852068372771
72-73	0.0	0.0	0.0	4.1153109024495595	0.002469643192475137
74-75	0.0	0.0	0.0	4.437179965695258	0.0025162402338425923
76-77	0.0	0.0	0.0	4.792272719435952	0.0025628372752100476
78-79	0.0	0.0	0.0	5.179657222844293	0.0026094343165775032
80-81	0.0	0.0	0.0	5.568532831576392	0.002702628399312414
82-83	0.0	0.0	0.0	5.969989641477704	0.003028807688884602
84-85	0.0	0.0	0.0	6.375383901374566	0.003028807688884602
86-87	0.0	0.0	0.0	6.791169301496371	0.003028807688884602
88	0.0	0.0	0.0	7.1227305493465	0.0030754047302520575
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2420	0.0	35.745914	1
CAACGCA	3110	0.0	29.918962	5
GTATCAA	3510	0.0	28.931477	1
ATCAACG	3295	0.0	28.66701	3
TAACGCC	670	0.0	26.653275	4
CTAACGC	685	0.0	26.069624	3
GTACATG	6215	0.0	25.870747	1
ACGCAGA	3715	0.0	24.7901	7
TACATGG	6320	0.0	24.686684	2
TATCAAC	4000	0.0	24.671812	2
GTACTAG	1265	0.0	24.52886	1
CGCCTAC	710	0.0	24.48637	7
TCTAACG	730	0.0	24.462595	2
ACATGGG	6325	0.0	24.295677	3
AACGCAG	4020	0.0	24.197239	6
CGCAGAG	3875	0.0	23.769833	8
GGCGTCG	850	0.0	23.22053	8
ATACGGG	870	0.0	22.686726	3
CATGGGG	4955	0.0	22.572262	4
CTGTGCG	1510	0.0	22.096483	9
>>END_MODULE