FastQCFastQC Report
Mon 16 Jan 2017
ERR1146549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146059
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.29113831446386146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57570.26825916715244086No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT38720.18042374417478738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28950.13489843475878344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42300.057.1771
GTATCAA79950.043.0815431
ATCAACG93650.035.7936253
CAACGCA93650.035.7434235
TATCAAC99550.034.360652
AACGCAG105700.032.5046046
TCAACGC110100.030.5311134
ACGCAGA110950.030.2019047
CGCAGAG111400.030.0377168
GCAGAGT123950.026.8826359
TCTACCG1406.356513E-726.864945
GTGGTAT12650.025.2939721
GAGTACT89250.023.66974612-13
CAGAGTA121500.023.20850210-11
GTACTAG13100.022.6291311
CTGTGCG14600.022.5329139
AGAGTAC117050.022.40448410-11
GGGCACC14150.021.92147394
GTACTTT96800.021.70222714-15
TAGTACT18100.021.5587444
Produced by FastQC (version 0.11.5)