##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2146059
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47077922834367	34.0	31.0	34.0	31.0	34.0
2	32.507168255858765	34.0	31.0	34.0	31.0	34.0
3	32.65406449682884	34.0	31.0	34.0	31.0	34.0
4	36.161881383503435	37.0	35.0	37.0	35.0	37.0
5	36.19793864008399	37.0	37.0	37.0	35.0	37.0
6	36.28777028031382	37.0	37.0	37.0	35.0	37.0
7	36.26649174137337	37.0	37.0	37.0	35.0	37.0
8	36.27571795556413	37.0	37.0	37.0	35.0	37.0
9	38.09613202619313	39.0	39.0	39.0	37.0	39.0
10-11	38.06591710665923	39.0	38.0	39.0	36.0	39.0
12-13	38.052964294085115	39.0	38.0	39.0	35.0	39.0
14-15	39.5034279113482	41.0	39.0	41.0	37.0	41.0
16-17	39.4998226982576	41.0	39.0	41.0	37.0	41.0
18-19	39.54286904507285	41.0	39.5	41.0	37.0	41.0
20-21	39.5739511355466	41.0	40.0	41.0	37.0	41.0
22-23	39.53375373184055	41.0	39.0	41.0	37.0	41.0
24-25	39.51691565795721	41.0	39.0	41.0	36.0	41.0
26-27	39.44024954579534	41.0	39.0	41.0	36.0	41.0
28-29	39.353737478792524	41.0	39.0	41.0	36.0	41.0
30-31	39.24374213383695	41.0	39.0	41.0	35.5	41.0
32-33	39.12025741137592	41.0	39.0	41.0	35.0	41.0
34-35	39.03714692839293	41.0	39.0	41.0	35.0	41.0
36-37	38.94627384428853	41.0	39.0	41.0	35.0	41.0
38-39	38.83002238055897	41.0	38.0	41.0	35.0	41.0
40-41	38.72923204814033	40.0	38.0	41.0	35.0	41.0
42-43	38.6244991400516	40.0	38.0	41.0	35.0	41.0
44-45	38.444796718077185	40.0	38.0	41.0	35.0	41.0
46-47	38.18515474178483	40.0	38.0	41.0	34.0	41.0
48-49	37.94105544162579	40.0	38.0	41.0	33.5	41.0
50-51	37.76517910271805	40.0	37.0	41.0	33.0	41.0
52-53	37.62126623732153	40.0	37.0	41.0	33.0	41.0
54-55	37.46615540392878	40.0	36.5	41.0	33.0	41.0
56-57	37.23927930219999	40.0	36.0	41.0	33.0	41.0
58-59	37.007032425483175	39.0	35.0	41.0	32.0	41.0
60-61	36.81941875782539	39.0	35.0	41.0	32.0	41.0
62-63	36.55530882422151	38.5	35.0	41.0	32.0	41.0
64-65	36.261193424784686	37.5	35.0	41.0	31.5	41.0
66-67	36.003371062957726	37.0	35.0	40.0	31.0	41.0
68-69	35.69117205072181	37.0	35.0	40.0	31.0	41.0
70-71	35.422474638395315	36.0	35.0	39.0	31.0	41.0
72-73	35.11033783320961	36.0	35.0	39.0	31.0	41.0
74-75	34.79996589096572	35.0	35.0	38.5	30.5	40.5
76-77	34.51338989282215	35.0	35.0	37.0	30.0	39.5
78-79	34.23680732915544	35.0	35.0	37.0	30.0	39.0
80-81	34.00053982672424	35.0	35.0	36.5	30.0	39.0
82-83	33.75152127690804	35.0	34.0	36.0	30.0	37.5
84-85	33.55242912706501	35.0	34.0	36.0	30.0	37.0
86-87	33.37901194701544	35.0	34.0	35.5	29.0	37.0
88-89	33.24156255722699	35.0	34.0	35.0	29.0	36.0
90-91	33.157634995123615	35.0	34.0	35.0	29.0	36.0
92-93	33.06893216822091	35.0	34.0	35.0	29.0	36.0
94-95	33.00213787225794	35.0	34.0	35.0	29.0	36.0
96-97	32.95557647762713	35.0	34.0	35.0	29.0	35.5
98-99	32.91050945011297	35.0	34.0	35.0	29.0	35.0
100	32.35134868146682	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18928411173168058
1101	2	0.22819618233103256
1101	3	0.28579463757850476
1101	4	0.17500754007332375
1101	5	0.1648358723055665
1101	6	0.1486366757023987
1101	7	0.17230232684917013
1101	8	0.1756999309563696
1101	9	0.20249047523581964
1101	10-11	0.22776570790244932
1101	12-13	0.2520629599249844
1101	14-15	0.37516432536220634
1101	16-17	0.36958321706429587
1101	18-19	0.287138750096986
1101	20-21	0.2961820496836509
1101	22-23	0.35760381170474886
1101	24-25	0.2834058207370589
1101	26-27	0.36423150248267433
1101	28-29	0.3442332434332087
1101	30-31	0.3697807697218778
1101	32-33	0.3401513838862513
1101	34-35	0.36635244767605
1101	36-37	0.3990117870691634
1101	38-39	0.5079438419695421
1101	40-41	0.37020289187888267
1101	42-43	0.3118267966665371
1101	44-45	0.3986980797329096
1101	46-47	0.42245809588690264
1101	48-49	0.4050835006029132
1101	50-51	0.3834505875564318
1101	52-53	0.4515251031449097
1101	54-55	0.39123570059501844
1101	56-57	0.4560800470282089
1101	58-59	0.5226219126168772
1101	60-61	0.526041351059618
1101	62-63	0.49221919467617425
1101	64-65	0.4924422890585447
1101	66-67	0.39827166265308733
1101	68-69	0.3887196748679713
1101	70-71	0.37634320518053244
1101	72-73	0.41762467698934813
1101	74-75	0.46917301448173987
1101	76-77	0.45911433243020383
1101	78-79	0.3462857547849225
1101	80-81	0.3837357208733252
1101	82-83	0.3771401726242729
1101	84-85	0.4433938964457411
1101	86-87	0.43036492357709477
1101	88-89	0.42153514599190345
1101	90-91	0.47578619989111104
1101	92-93	0.40769549680912576
1101	94-95	0.33655679051582155
1101	96-97	0.3362296864138372
1101	98-99	0.37742352626077036
1101	100	0.49082187027984503
1102	1	0.20520508749604005
1102	2	0.22747646860822357
1102	3	0.2418464531823119
1102	4	0.1321224885013308
1102	5	0.11272864607704491
1102	6	0.18692885156514194
1102	7	0.15613916020340213
1102	8	0.1696534369295435
1102	9	0.15865983856235033
1102	10-11	0.21657561407713644
1102	12-13	0.20509957000949441
1102	14-15	0.31137446379415934
1102	16-17	0.3014185461860919
1102	18-19	0.2600201120422625
1102	20-21	0.257960000379839
1102	22-23	0.20754692995964774
1102	24-25	0.20840292343403632
1102	26-27	0.27397968545977847
1102	28-29	0.31311242471450385
1102	30-31	0.3439728952070311
1102	32-33	0.31405117740906974
1102	34-35	0.3805992553211439
1102	36-37	0.37106759503828357
1102	38-39	0.37733377638406296
1102	40-41	0.37145606413005083
1102	42-43	0.3672940666029447
1102	44-45	0.38618858958729163
1102	46-47	0.40203116350404144
1102	48-49	0.35952358792408035
1102	50-51	0.3399712932211898
1102	52-53	0.33346118745760833
1102	54-55	0.30132375495551855
1102	56-57	0.3177333545656893
1102	58-59	0.3682398896134771
1102	60-61	0.41042630684322745
1102	62-63	0.3779134949124554
1102	64-65	0.415543018232853
1102	66-67	0.41749319872036494
1102	68-69	0.41904769687328525
1102	70-71	0.3591266028063984
1102	72-73	0.40688336948971227
1102	74-75	0.43621495087352713
1102	76-77	0.4189215257831975
1102	78-79	0.34631023403905203
1102	80-81	0.3796890754772022
1102	82-83	0.4564491717657191
1102	84-85	0.38661337956687447
1102	86-87	0.3873281479324149
1102	88-89	0.35409255398184314
1102	90-91	0.42165783872395934
1102	92-93	0.48014935873666786
1102	94-95	0.39403857298394485
1102	96-97	0.32028588699708394
1102	98-99	0.3207965073135952
1102	100	0.32446346088737954
1103	1	0.1696454203848461
1103	2	0.15431023336999772
1103	3	0.19637813747213784
1103	4	0.17876365012210726
1103	5	0.04400035055216733
1103	6	0.0756515345237645
1103	7	0.080596368777897
1103	8	0.10973964822652249
1103	9	0.1151118799671309
1103	10-11	0.1348289663627753
1103	12-13	0.18785339321412664
1103	14-15	0.24090802962322755
1103	16-17	0.21880698396720533
1103	18-19	0.2266754380328848
1103	20-21	0.21868325456664195
1103	22-23	0.23763286110314397
1103	24-25	0.2301421803198167
1103	26-27	0.2720780250281081
1103	28-29	0.2747516976735014
1103	30-31	0.33175784749903414
1103	32-33	0.26163802320618856
1103	34-35	0.3449325126015168
1103	36-37	0.34715554613583066
1103	38-39	0.2778901540660854
1103	40-41	0.30663110188547904
1103	42-43	0.2796129729027612
1103	44-45	0.2764877293974095
1103	46-47	0.27279155701996416
1103	48-49	0.29869906300383064
1103	50-51	0.3160846480661945
1103	52-53	0.3008507292333533
1103	54-55	0.21853786842289935
1103	56-57	0.19801712629568158
1103	58-59	0.21829112446115317
1103	60-61	0.18710890743672337
1103	62-63	0.2943741023659143
1103	64-65	0.2559749579134376
1103	66-67	0.31710281197324264
1103	68-69	0.28201655923886904
1103	70-71	0.1949543506787137
1103	72-73	0.24959488002265573
1103	74-75	0.20866385076067928
1103	76-77	0.18043583367234106
1103	78-79	0.20764494693040803
1103	80-81	0.2351380724683949
1103	82-83	0.20479407013529993
1103	84-85	0.12569617845205272
1103	86-87	0.15242005133074343
1103	88-89	0.1423555240787806
1103	90-91	0.172311569739108
1103	92-93	0.17931758781294604
1103	94-95	0.14837311707096035
1103	96-97	0.14370206424591458
1103	98-99	0.12944580668061434
1103	100	0.13017785581403984
1104	1	0.1968730669041392
1104	2	0.17060614334808122
1104	3	0.2325771464743056
1104	4	0.21207317449605512
1104	5	0.16170731070462807
1104	6	0.1115825638965049
1104	7	0.11997093638876066
1104	8	0.12812179107179844
1104	9	0.176219022918346
1104	10-11	0.1616079187845969
1104	12-13	0.22706179055050768
1104	14-15	0.28245426292330933
1104	16-17	0.2707049038841305
1104	18-19	0.2548144443346416
1104	20-21	0.24674169519776257
1104	22-23	0.24769704222439515
1104	24-25	0.2697224280223196
1104	26-27	0.2829681331097902
1104	28-29	0.3204896935317194
1104	30-31	0.27831486468878097
1104	32-33	0.28740477992087676
1104	34-35	0.2459053359169303
1104	36-37	0.2334409098557657
1104	38-39	0.3387118529034723
1104	40-41	0.35793826414496976
1104	42-43	0.3555679051422729
1104	44-45	0.32268976519513615
1104	46-47	0.39907186119287275
1104	48-49	0.38963686229785566
1104	50-51	0.24573381119673599
1104	52-53	0.2346737323527961
1104	54-55	0.3071808917278531
1104	56-57	0.287112336594987
1104	58-59	0.26402923484058505
1104	60-61	0.24701878840681957
1104	62-63	0.20446722299671904
1104	64-65	0.19909009742259798
1104	66-67	0.14964211250288884
1104	68-69	0.15828034372735544
1104	70-71	0.15884919180744816
1104	72-73	0.17639527081482242
1104	74-75	0.1657033377881163
1104	76-77	0.20115197602083157
1104	78-79	0.21585697653597435
1104	80-81	0.16153466147708428
1104	82-83	0.20862635061789803
1104	84-85	0.18588400239676162
1104	86-87	0.1720868931003423
1104	88-89	0.17717501059544816
1104	90-91	0.08463716682120292
1104	92-93	0.15085408566504555
1104	94-95	0.14488199527043832
1104	96-97	0.10204944074251898
1104	98-99	0.12389965904238665
1104	100	0.09676434618217655
1105	1	0.17968807085294713
1105	2	0.1648544890645809
1105	3	0.12722056499461587
1105	4	0.10445752800283259
1105	5	0.12282877306824247
1105	6	0.07586211672239784
1105	7	0.13701983263853634
1105	8	0.11610187832902596
1105	9	0.17265646684906244
1105	10-11	0.16855044633042837
1105	12-13	0.15074526458420223
1105	14-15	0.20839248894866103
1105	16-17	0.20818621412266936
1105	18-19	0.19055425218472521
1105	20-21	0.23318347131933592
1105	22-23	0.29245087410471626
1105	24-25	0.30668454627196695
1105	26-27	0.23467522716816802
1105	28-29	0.20966659643696772
1105	30-31	0.2298614184884542
1105	32-33	0.25350682410155656
1105	34-35	0.24377805768293115
1105	36-37	0.263751869148507
1105	38-39	0.2703164312241171
1105	40-41	0.2703460613217601
1105	42-43	0.26969563959814735
1105	44-45	0.259215501475893
1105	46-47	0.23458614592131966
1105	48-49	0.296874783919705
1105	50-51	0.2833356943579233
1105	52-53	0.23329787837446503
1105	54-55	0.2571651731592368
1105	56-57	0.27439131818509566
1105	58-59	0.22334066569038669
1105	60-61	0.24861652339941998
1105	62-63	0.2656594189190926
1105	64-65	0.18813837643102715
1105	66-67	0.23720268649901044
1105	68-69	0.23533535488370205
1105	70-71	0.28644754787519844
1105	72-73	0.2030253055490263
1105	74-75	0.18802466366854986
1105	76-77	0.18312689915320135
1105	78-79	0.21455444392952927
1105	80-81	0.25702975805044304
1105	82-83	0.27943323075039217
1105	84-85	0.30934281591319746
1105	86-87	0.24476993774874245
1105	88-89	0.2962172897112225
1105	90-91	0.2716052274565399
1105	92-93	0.23973684047837196
1105	94-95	0.26856125212718496
1105	96-97	0.22123165345693252
1105	98-99	0.2887941529428204
1105	100	0.3000063968036315
1106	1	0.1514539083519253
1106	2	0.18078039710925253
1106	3	0.22301060161267827
1106	4	0.15023660891166912
1106	5	0.06609883150694174
1106	6	0.042337130690413005
1106	7	0.1282028594183373
1106	8	0.08922789498170403
1106	9	0.12222791499162611
1106	10-11	0.13553946049816545
1106	12-13	0.10090885746261335
1106	14-15	0.16642340550617973
1106	16-17	0.17260667154724985
1106	18-19	0.16416914930834992
1106	20-21	0.19462246968279828
1106	22-23	0.17128588813875467
1106	24-25	0.19571646803127862
1106	26-27	0.1415079068727536
1106	28-29	0.22032587222951605
1106	30-31	0.19745088186089532
1106	32-33	0.18169581235521548
1106	34-35	0.16645948092653384
1106	36-37	0.20940876604599623
1106	38-39	0.2043731199012413
1106	40-41	0.17717966947627417
1106	42-43	0.15878847798926188
1106	44-45	0.17462814176467134
1106	46-47	0.14290702770942687
1106	48-49	0.0467510421756927
1106	50-51	0.09105536354915245
1106	52-53	0.17845686820443518
1106	54-55	0.12701547401827895
1106	56-57	0.19287975836444105
1106	58-59	0.1621233708551273
1106	60-61	0.07177311700326072
1106	62-63	0.06669847818886154
1106	64-65	0.17534222153421553
1106	66-67	0.10811209083028928
1106	68-69	0.19644253555676983
1106	70-71	0.12359681043836446
1106	72-73	0.06621578344903156
1106	74-75	0.07215834147744715
1106	76-77	0.060186046365743096
1106	78-79	0.06901156068396119
1106	80-81	0.0374578477461327
1106	82-83	0.09647025609584148
1106	84-85	0.12412520375438874
1106	86-87	0.13966465977907916
1106	88-89	0.014279540422421633
1106	90-91	0.1505353044477289
1106	92-93	0.0659516450045885
1106	94-95	0.134085079453655
1106	96-97	0.0758183405577384
1106	98-99	0.10202829893805898
1106	100	0.027823767813053735
1107	1	0.07754495815169804
1107	2	0.08940885201429438
1107	3	0.13843344513772848
1107	4	0.0787562757666933
1107	5	-0.0175202629087039
1107	6	0.03830715746976665
1107	7	0.03139947883213523
1107	8	0.0498782647333158
1107	9	0.06664911244635618
1107	10-11	0.05640504780130584
1107	12-13	0.08570948640888787
1107	14-15	0.1643977562911374
1107	16-17	0.10558374940392667
1107	18-19	0.09714141179514257
1107	20-21	0.06191215348988521
1107	22-23	0.10570708559348674
1107	24-25	0.06168742840274888
1107	26-27	0.06168745137807008
1107	28-29	0.11343473398417814
1107	30-31	0.11496895178203204
1107	32-33	0.08670767880241215
1107	34-35	0.07400216819773675
1107	36-37	0.0878896151234585
1107	38-39	0.10220522072918925
1107	40-41	0.11794683651556426
1107	42-43	0.1505875100016496
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2216	38-39	-0.5725517287237096
2216	40-41	-0.5631423678776954
2216	42-43	-0.6037455279756472
2216	44-45	-0.6160438136207631
2216	46-47	-0.5937184905668502
2216	48-49	-0.5474598863962328
2216	50-51	-0.5270422394415348
2216	52-53	-0.5269408282237791
2216	54-55	-0.5213712548579394
2216	56-57	-0.4591128412394383
2216	58-59	-0.37899487471985793
2216	60-61	-0.45786085103392793
2216	62-63	-0.45722567048881047
2216	64-65	-0.38755909205833916
2216	66-67	-0.45372266086381785
2216	68-69	-0.49321438931410455
2216	70-71	-0.46099927565762755
2216	72-73	-0.4166589080193148
2216	74-75	-0.4337941622094519
2216	76-77	-0.40269848081220516
2216	78-79	-0.3920474225161854
2216	80-81	-0.43523642227712145
2216	82-83	-0.4061812188370908
2216	84-85	-0.4133406704999629
2216	86-87	-0.41063862932292494
2216	88-89	-0.3394636888645124
2216	90-91	-0.3757220446351681
2216	92-93	-0.380812405682164
2216	94-95	-0.33524724475549306
2216	96-97	-0.416797091496079
2216	98-99	-0.41610881458404236
2216	100	-0.58065509982778
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	20.0
8	68.0
9	91.0
10	110.0
11	155.0
12	116.0
13	127.0
14	206.0
15	396.0
16	852.0
17	1644.0
18	2960.0
19	4677.0
20	6896.0
21	10078.0
22	15081.0
23	17092.0
24	15807.0
25	13171.0
26	11069.0
27	12795.0
28	15629.0
29	18337.0
30	20860.0
31	26686.0
32	35043.0
33	46853.0
34	69111.0
35	113305.0
36	209217.0
37	516421.0
38	689178.0
39	271349.0
40	658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79787962285973	20.892299361724636	13.56655524691056	23.74326576850507
2	15.471336801328995	21.364177787478464	39.66541024112737	23.499075170065172
3	17.488850461754673	27.082835326061378	31.365954149315044	24.062360062868905
4	12.473981377026448	18.50084270749313	39.583161506743295	29.442014408737133
5	12.614343285077979	38.32797637499796	35.71589396718698	13.341786372737083
6	28.471173413397217	39.31786949667583	18.42104650008644	13.789910589840504
7	24.657453180414127	33.981017279596955	23.850158686372264	17.511370853616658
8	25.22959527207779	34.401104536268576	21.412598628462685	18.956701563190947
9	26.06522001492037	14.625133791755026	21.80182371500504	37.50782247831956
10-11	24.00768105629901	26.352234491223214	29.05122832130897	20.588856131168807
12-13	25.209045976834748	24.53425092227194	28.67465433149788	21.58204876939544
14-15	22.563247926372927	25.48376477155679	27.050660548163062	24.90232675390722
16-17	21.292168575048496	28.139044639499662	27.967451034664002	22.60133575078784
18-19	20.97314193132621	27.245336684592548	30.677185482784957	21.104335901296285
20-21	22.267607740514123	26.3876249441418	30.162614354964145	21.182152960379934
22-23	22.417218805348806	26.228991043540944	29.846639102835027	21.507151048275226
24-25	22.117705058435018	26.371875144159596	30.194579925342218	21.315839872063165
26-27	21.957248146486187	26.603299350111065	29.785085125805022	21.654367377597726
28-29	21.53356956485576	26.817861675252562	30.080470779338032	21.568097980553652
30-31	21.89235087349791	26.425900914491727	30.08002355950254	21.601724652507823
32-33	21.999948276476676	26.730507163125505	29.49258990631752	21.7769546540803
34-35	22.100170034822423	26.658585081017005	29.776853176864638	21.464391707295935
36-37	22.145173368076378	26.33863938293816	29.714367060088787	21.80182018889667
38-39	22.19890281788389	26.198845969069584	29.99371401225418	21.60853720079234
40-41	22.05664269140504	26.02485098657685	30.072974231738907	21.845532090279196
42-43	22.23466110730049	26.151418793987837	29.429700547238458	22.18421955147322
44-45	22.179942936944062	26.67029193675628	29.318835980759044	21.830929145540615
46-47	22.48305783901272	26.445660786798452	28.590017527538365	22.481263846650464
48-49	22.809940499960625	26.48255002565179	28.502378091087387	22.205131383300195
50-51	22.625337975976766	26.62180124158482	28.13761111997754	22.615249662460872
52-53	22.213469658971555	26.904602807592408	28.141300387038815	22.740627146397223
54-55	22.223438938338045	26.898230524575244	27.964035010669697	22.914295526417018
56-57	22.83995373809087	26.69081033828788	27.632380913327886	22.83685501029337
58-59	22.020491069930774	26.879000451070077	28.37762021390414	22.722888265095005
60-61	22.309129852207843	27.30428250801891	27.872007746431127	22.51457989334212
62-63	22.533762807033895	26.938951439571767	27.60368819277389	22.923597560620447
64-65	21.848892997871364	27.08473906511536	27.75494693323691	23.31142100377637
66-67	22.355541669083983	26.848677301933133	27.688855430880206	23.106925598102677
68-69	22.105531712636125	26.61375402494886	27.992604811765197	23.288109450649817
70-71	22.14889288387	26.465662578179387	27.81450457729776	23.570939960652854
72-73	22.471662979110476	26.438356611225956	27.865226500450145	23.224753909213423
74-75	22.426927065579772	26.82405948398163	27.69186428917756	23.05714916126104
76-77	22.318857125549137	26.651048407671507	27.41521696837244	23.614877498406912
78-79	22.20075893220145	26.890812689316583	27.336330634799	23.572097743682964
80-81	22.154061073838637	26.857358934381082	27.655030758721338	23.33354923305894
82-83	22.560354361923242	26.116578120453138	28.25459465265211	23.068472864971508
84-85	22.6765878073136	26.597266521811864	27.327256452181835	23.3988892186927
86-87	21.857908346860068	26.87559542295795	27.759471608066605	23.50702462211538
88-89	22.221481845284416	26.27315062901493	27.677146055038687	23.828221470661965
90-91	22.109107904302725	26.286928737746724	27.646420718162922	23.95754263978763
92-93	22.44171758558362	26.211884202624436	27.667738864588532	23.67865934720341
94-95	21.61112532320873	26.26470194901445	28.08897611855033	24.035196609226496
96-97	21.890791377734473	26.15959052183583	27.83334113837595	24.116276962053746
98-99	21.66221974118055	26.480462728768924	27.603944359223355	24.253373170827174
100	22.034678611718558	26.005510591789843	27.36848040282398	24.59133039366762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	94.0
2	106.5
3	152.0
4	92.0
5	84.0
6	181.5
7	447.0
8	868.5
9	1067.5
10	822.0
11	610.0
12	654.5
13	721.0
14	875.5
15	1122.5
16	1400.5
17	1738.5
18	2211.5
19	2647.0
20	3072.5
21	3652.5
22	4275.5
23	5051.5
24	6111.0
25	7687.0
26	9848.5
27	12528.5
28	15337.0
29	19454.5
30	24284.5
31	28703.0
32	35326.0
33	41028.0
34	45284.0
35	49761.5
36	54495.5
37	61561.0
38	65050.5
39	65703.5
40	66217.5
41	66081.0
42	67215.5
43	70353.5
44	75054.5
45	83127.5
46	89467.5
47	92245.5
48	93754.0
49	90100.0
50	85236.5
51	83901.5
52	82511.0
53	79899.5
54	76657.5
55	68586.0
56	61348.5
57	52018.0
58	41647.0
59	37056.5
60	30319.5
61	22475.0
62	16965.0
63	12844.0
64	8922.0
65	5137.5
66	3446.0
67	2476.0
68	1702.5
69	1272.0
70	939.0
71	658.5
72	558.0
73	557.5
74	464.5
75	296.5
76	167.0
77	104.5
78	56.0
79	18.5
80	7.0
81	2.0
82	1.5
83	2.0
84	1.0
85	0.5
86	1.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005591644964094649
2	0.08764903481218363
3	2.795822482047325E-4
4	0.0
5	0.04072581415515603
6	0.004939286384950274
7	9.319408273491083E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.3298520683727708E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.6597041367455415E-4
24-25	0.0
26-27	0.0
28-29	2.3298520683727708E-5
30-31	1.6308964478609395E-4
32-33	0.0015610008858097562
34-35	0.0013047171582887516
36-37	8.387467446141974E-4
38-39	7.455526618792866E-4
40-41	0.001281418637605024
42-43	5.125674550420095E-4
44-45	5.59164496409465E-4
46-47	3.494778102559156E-4
48-49	4.6597041367455415E-4
50-51	5.358659757257372E-4
52-53	9.785378687165638E-4
54-55	0.001281418637605024
56-57	7.92149703246742E-4
58-59	0.002376449109740226
60-61	0.0017939860926470335
62-63	0.0010018363894002914
64-65	0.003867554433498799
66-67	0.003984047036917438
68-69	0.0032151958543544237
70-71	0.0015377023651260288
72-73	0.004799495260847908
74-75	0.0032384943750381513
76-77	0.003098703250935785
78-79	8.154482239304697E-4
80-81	4.6597041367455415E-5
82-83	2.0968668615354934E-4
84-85	9.319408273491083E-5
86-87	2.3298520683727708E-5
88-89	2.3298520683727708E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	4.892689343582819E-4
98-99	3.261792895721879E-4
100	4.6597041367455415E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2146059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.147829936616155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.65009892395499	34.75658023945048
2	16.905720983661737	17.63193325623751
3	6.849358257512611	10.715375087631756
4	3.522658563390329	7.347959987537737
5	1.9828805331321464	5.170145841320097
6	1.1488407631204494	3.5945731643674512
7	0.7754530184066395	2.830673448939358
8	0.519036651793668	2.1653308038885486
9	0.3731023303065987	1.7510829182805325
>10	1.2335603225875242	9.605859580519589
>50	0.0222816039775008	0.831255460267549
>100	0.015040198099793088	1.6424303814396035
>500	0.0011244857462766155	0.3598265794897224
>1k	5.622428731383078E-4	0.7170143818636107
>5k	2.811214365691539E-4	0.8799588687663715
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6248	0.29113831446386146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5757	0.26825916715244086	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	3872	0.18042374417478738	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2895	0.13489843475878344	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.6597041367455415E-5
7	0.0	0.0	0.0	0.0	4.6597041367455415E-5
8	0.0	0.0	0.0	4.6597041367455415E-5	4.6597041367455415E-5
9	0.0	0.0	0.0	4.6597041367455415E-5	9.319408273491083E-5
10-11	0.0	0.0	0.0	4.6597041367455415E-5	9.319408273491083E-5
12-13	0.0	0.0	0.0	4.6597041367455415E-5	9.319408273491083E-5
14-15	0.0	0.0	0.0	4.6597041367455415E-5	1.8638816546982166E-4
16-17	0.0	0.0	0.0	6.989556205118312E-5	3.494778102559156E-4
18-19	0.0	0.0	0.0	9.319408273491083E-5	4.1937337230709874E-4
20-21	0.0	0.0	0.0	1.6308964478609395E-4	4.426718929908264E-4
22-23	0.0	0.0	0.0	6.989556205118312E-4	6.057615377769205E-4
24-25	0.0	0.0	0.0	0.0017007920099121226	8.154482239304697E-4
26-27	0.0	0.0	0.0	0.0032151958543544237	8.853437859816528E-4
28-29	0.0	0.0	0.0	0.00934270679417481	8.853437859816528E-4
30-31	0.0	0.0	0.0	0.02644382097603095	9.319408273491083E-4
32-33	0.0	0.0	0.0	0.06460679785597694	0.0011416275135026576
34-35	0.0	0.0	0.0	0.12616148950238554	0.0011649260341863854
36-37	0.0	0.0	0.0	0.2218019169090878	0.0011649260341863854
38-39	0.0	0.0	0.0	0.3457733454672029	0.001188224554870113
40-41	0.0	0.0	0.0	0.4705136252078811	0.0012581201169212962
42-43	0.0	0.0	0.0	0.5986554889683835	0.0012814186376050238
44-45	0.0	0.0	0.0	0.7558040109801268	0.0013280156789724795
46-47	0.0	0.0	0.0	0.9406078770434549	0.0015377023651260285
48-49	0.0	0.0	0.0	1.131585851087971	0.0017706875719633057
50-51	0.0	0.0	0.0	1.3176012402268529	0.0017706875719633057
52-53	0.0	0.0	0.0	1.4983744622118964	0.0018638816546982166
54-55	0.0	0.0	0.0	1.6930336025244412	0.001980374258116855
56-57	0.0	0.0	0.0	1.9199611939839492	0.002003672778800583
58-59	0.0	0.0	0.0	2.1648985419319784	0.002050269820168038
60-61	0.0	0.0	0.0	2.4146819821822234	0.0020968668615354937
62-63	0.0	0.0	0.0	2.6619957792399926	0.0020968668615354937
64-65	0.0	0.0	0.0	2.9183727008437326	0.0020968668615354937
66-67	0.0	0.0	0.0	3.204152355550337	0.0020968668615354937
68-69	0.0	0.0	0.0	3.508733916448709	0.0020968668615354937
70-71	0.0	0.0	0.0	3.8251977228957825	0.0022599565063215876
72-73	0.0	0.0	0.0	4.142593470170205	0.002469643192475137
74-75	0.0	0.0	0.0	4.466838982525644	0.002469643192475137
76-77	0.0	0.0	0.0	4.82544981288958	0.0025162402338425923
78-79	0.0	0.0	0.0	5.215443750614499	0.002562837275210048
80-81	0.0	0.0	0.0	5.608280107862832	0.0028191210027310528
82-83	0.0	0.0	0.0	6.012789023973712	0.003168598812986968
84-85	0.0	0.0	0.0	6.421771256055868	0.003191897333670696
86-87	0.0	0.0	0.0	6.840492269783822	0.0032151958543544237
88	0.0	0.0	0.0	7.175851176505399	0.0032151958543544237
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4230	0.0	57.177	1
GTATCAA	7995	0.0	43.081543	1
ATCAACG	9365	0.0	35.793625	3
CAACGCA	9365	0.0	35.743423	5
TATCAAC	9955	0.0	34.36065	2
AACGCAG	10570	0.0	32.504604	6
TCAACGC	11010	0.0	30.531113	4
ACGCAGA	11095	0.0	30.201904	7
CGCAGAG	11140	0.0	30.037716	8
GCAGAGT	12395	0.0	26.882635	9
TCTACCG	140	6.356513E-7	26.86494	5
GTGGTAT	1265	0.0	25.293972	1
GAGTACT	8925	0.0	23.669746	12-13
CAGAGTA	12150	0.0	23.208502	10-11
GTACTAG	1310	0.0	22.629131	1
CTGTGCG	1460	0.0	22.532913	9
AGAGTAC	11705	0.0	22.404484	10-11
GGGCACC	1415	0.0	21.921473	94
GTACTTT	9680	0.0	21.702227	14-15
TAGTACT	1810	0.0	21.558744	4
>>END_MODULE