FastQCFastQC Report
Mon 16 Jan 2017
ERR1146548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168195
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.038.016421
GTATCAA40600.034.0456851
CAACGCA40550.031.8670755
ATCAACG41150.031.289683
TATCAAC50050.027.134022
CGTTAAC4200.026.8659841
AACGCAG54300.026.3930956
GTGATCG11500.026.1512558
ACGCAGA49600.026.0514147
CGCAGAG49650.026.0269818
GTACTAG11950.025.9667051
GCATCGT10800.025.6707538
TATTCGC2800.025.17359
TCGCCGG1151.3768378E-424.52415394
TGATCGC12650.024.51689
GTACATG83750.024.4199771
TACATGG83450.024.382712
ACGTTAA3300.024.2072418
GTCCTAC12100.024.0905721
ACATGGG84100.023.8031233
Produced by FastQC (version 0.11.5)