##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146548_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2168195 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23818199008853 31.0 31.0 34.0 28.0 34.0 2 31.319181162210963 31.0 31.0 34.0 28.0 34.0 3 31.51489141889913 33.0 31.0 34.0 28.0 34.0 4 35.03191871579816 37.0 35.0 37.0 32.0 37.0 5 35.000296560041875 37.0 35.0 37.0 32.0 37.0 6 35.07598394055885 37.0 35.0 37.0 32.0 37.0 7 34.90372729390115 37.0 35.0 37.0 32.0 37.0 8 34.87845696535597 37.0 35.0 37.0 32.0 37.0 9 36.4429767617765 39.0 37.0 39.0 32.0 39.0 10-11 36.45137499164051 39.0 37.0 39.0 32.0 39.0 12-13 36.42458658008159 39.0 37.0 39.0 32.0 39.0 14-15 37.77021324189015 40.0 38.0 41.0 32.0 41.0 16-17 37.78013186083355 40.0 38.0 41.0 32.0 41.0 18-19 37.807162870498274 40.0 38.0 41.0 32.0 41.0 20-21 37.920283230982456 40.0 38.0 41.0 32.0 41.0 22-23 37.94527521740434 40.0 38.0 41.0 32.0 41.0 24-25 38.00187275590987 40.0 38.0 41.0 32.0 41.0 26-27 38.04000401255422 40.0 38.0 41.0 32.0 41.0 28-29 38.02007337900881 40.0 38.0 41.0 32.0 41.0 30-31 38.025682653082406 40.0 38.0 41.0 32.0 41.0 32-33 38.049814477018906 40.0 38.0 41.0 32.0 41.0 34-35 37.992501366343895 40.0 38.0 41.0 32.0 41.0 36-37 37.89370697746282 40.0 38.0 41.0 31.0 41.0 38-39 37.84613561049629 40.0 38.0 41.0 31.0 41.0 40-41 37.78446126847447 40.0 38.0 41.0 31.0 41.0 42-43 37.6437947232606 40.0 37.0 41.0 31.0 41.0 44-45 37.469128007397856 40.0 37.0 41.0 30.5 41.0 46-47 37.361244030172564 40.0 37.0 41.0 30.0 41.0 48-49 37.23838953599653 40.0 36.0 41.0 30.0 41.0 50-51 37.089819642605946 40.0 36.0 41.0 30.0 41.0 52-53 36.95481633340175 39.5 35.0 41.0 30.0 41.0 54-55 36.7875698449632 39.0 35.0 41.0 30.0 41.0 56-57 36.633196045558634 39.0 35.0 41.0 30.0 41.0 58-59 36.39735840180427 39.0 35.0 40.5 30.0 41.0 60-61 36.23181609587699 38.0 35.0 40.0 30.0 41.0 62-63 35.99808181459693 37.5 35.0 40.0 29.5 41.0 64-65 35.75565274341099 37.0 35.0 40.0 29.0 41.0 66-67 35.52159653536698 37.0 34.5 40.0 29.0 41.0 68-69 35.24509211579217 36.0 34.0 39.5 29.0 41.0 70-71 35.04083857771096 36.0 34.0 39.0 29.0 41.0 72-73 34.75547102543821 35.0 34.0 39.0 29.0 41.0 74-75 34.4554963921603 35.0 34.0 38.5 28.0 40.0 76-77 34.169032997493304 35.0 34.0 37.0 28.0 39.5 78-79 33.89959274880719 35.0 34.0 37.0 28.0 39.0 80-81 33.65459656534583 35.0 34.0 36.5 28.0 39.0 82-83 33.392936059717876 35.0 33.0 36.0 27.0 37.5 84-85 33.16921586849891 35.0 33.0 36.0 27.0 37.0 86-87 32.96902492626356 35.0 33.0 35.0 27.0 37.0 88-89 32.834849494625715 35.0 33.0 35.0 27.0 36.5 90-91 32.655730919036344 35.0 33.0 35.0 26.5 36.0 92-93 32.541726412984076 35.0 33.0 35.0 26.0 36.0 94-95 32.44941506644928 35.0 33.0 35.0 26.0 36.0 96-97 32.36600905361372 35.0 33.0 35.0 26.0 35.5 98-99 32.29074253007686 35.0 33.0 35.0 25.5 35.0 100 31.328634186500754 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3970454066144349 1101 2 0.34072352854525434 1101 3 0.4898130154659377 1101 4 0.43489951795081083 1101 5 0.41390972656223113 1101 6 0.3410485144022246 1101 7 0.40975017482971765 1101 8 0.41087984162913926 1101 9 0.46595228238314235 1101 10-11 0.5836724325317277 1101 12-13 0.5318381334185602 1101 14-15 0.6698407008650094 1101 16-17 0.6274215342880183 1101 18-19 0.5906241028124342 1101 20-21 0.5332394060427319 1101 22-23 0.624952802663266 1101 24-25 0.605737551852009 1101 26-27 0.6015253182154865 1101 28-29 0.569542467765487 1101 30-31 0.5998335615095627 1101 32-33 0.5533526962652289 1101 34-35 0.5714920750760868 1101 36-37 0.6199886472136811 1101 38-39 0.6569964819944261 1101 40-41 0.5954145703066587 1101 42-43 0.5931291593763035 1101 44-45 0.4486306356869534 1101 46-47 0.4390326793352344 1101 48-49 0.43973122293518685 1101 50-51 0.48589734059947176 1101 52-53 0.511759130707901 1101 54-55 0.4998533589015466 1101 56-57 0.3236777595610363 1101 58-59 0.3865376712556312 1101 60-61 0.40887158982756944 1101 62-63 0.382478185295966 1101 64-65 0.4496360742598142 1101 66-67 0.4096068702813014 1101 68-69 0.33497429724858563 1101 70-71 0.41686274857167405 1101 72-73 0.4341643855033652 1101 74-75 0.41744370941802345 1101 76-77 0.4376698907311365 1101 78-79 0.43444766562347326 1101 80-81 0.4390851373493021 1101 82-83 0.40834141064737395 1101 84-85 0.43430044549035784 1101 86-87 0.3300933878971293 1101 88-89 0.307362100684891 1101 90-91 0.3163548057033623 1101 92-93 0.33588305616316916 1101 94-95 0.45947372578707046 1101 96-97 0.29288835796902646 1101 98-99 0.34761445412729586 1101 100 0.2680492405401935 1102 1 0.3066661754403057 1102 2 0.2828007081106847 1102 3 0.3242923611303574 1102 4 0.3051878020505896 1102 5 0.3536599779698051 1102 6 0.330122781531216 1102 7 0.2886065417393624 1102 8 0.31194328031129714 1102 9 0.46002935544597534 1102 10-11 0.4618693025842262 1102 12-13 0.47273136763620727 1102 14-15 0.5789735034604035 1102 16-17 0.5904176418228673 1102 18-19 0.4694663888497388 1102 20-21 0.446975757837194 1102 22-23 0.4774927872203847 1102 24-25 0.43370551812345326 1102 26-27 0.5001863040608825 1102 28-29 0.5006708730324476 1102 30-31 0.558573149344511 1102 32-33 0.46906689859149964 1102 34-35 0.4853100959301173 1102 36-37 0.5444459191905935 1102 38-39 0.5298442846614932 1102 40-41 0.44833512244069595 1102 42-43 0.4004197486428893 1102 44-45 0.34025707372804703 1102 46-47 0.42438370780214996 1102 48-49 0.4142317871941401 1102 50-51 0.3898588788246897 1102 52-53 0.3668272718893846 1102 54-55 0.3181411094642641 1102 56-57 0.29849155922555326 1102 58-59 0.3672935990468673 1102 60-61 0.39796736228886687 1102 62-63 0.35497606548463523 1102 64-65 0.21934404720815337 1102 66-67 0.24708057745240097 1102 68-69 0.25040521802499427 1102 70-71 0.21425102837038423 1102 72-73 0.2755491189703747 1102 74-75 0.1630317880906702 1102 76-77 0.19310638753298548 1102 78-79 0.24873952063247629 1102 80-81 0.2537136532151223 1102 82-83 0.2666869388695332 1102 84-85 0.21387130146402455 1102 86-87 0.22308234234883173 1102 88-89 0.26984208024458667 1102 90-91 0.31780551957798764 1102 92-93 0.25066804892303196 1102 94-95 0.2682375583240244 1102 96-97 0.2585384539237765 1102 98-99 0.23844378678611378 1102 100 0.28961243242305557 1103 1 0.21105763579230086 1103 2 0.28542297204895917 1103 3 0.3315161891517846 1103 4 0.2820393411814024 1103 5 0.1917971825618423 1103 6 0.21416271132189024 1103 7 0.20863152432313115 1103 8 0.3063736159026078 1103 9 0.22869420874134505 1103 10-11 0.30203004101213793 1103 12-13 0.21867686467167147 1103 14-15 0.31841601780925544 1103 16-17 0.2649025398610547 1103 18-19 0.20122774165967883 1103 20-21 0.3137348069665862 1103 22-23 0.3769451968097499 1103 24-25 0.2862025414858991 1103 26-27 0.26860049977391753 1103 28-29 0.3164912353504832 1103 30-31 0.33063681359793407 1103 32-33 0.3865336363681209 1103 34-35 0.4171646293415492 1103 36-37 0.3692119469093669 1103 38-39 0.3777321826260902 1103 40-41 0.3298853633441894 1103 42-43 0.3089354020765853 1103 44-45 0.28536747396355366 1103 46-47 0.20892782605707794 1103 48-49 0.20987188272882662 1103 50-51 0.2694841350272341 1103 52-53 0.2793395842301578 1103 54-55 0.32261085462779704 1103 56-57 0.22925348266269907 1103 58-59 0.29431998097794576 1103 60-61 0.22360823411852948 1103 62-63 0.16439867341232173 1103 64-65 0.2678828987553459 1103 66-67 0.26269002949905484 1103 68-69 0.2385081194526748 1103 70-71 0.16516234322251933 1103 72-73 0.22169126059682753 1103 74-75 0.19047142107454107 1103 76-77 0.2076685974935515 1103 78-79 0.1863928262880421 1103 80-81 0.23460381654960116 1103 82-83 0.24594335414096946 1103 84-85 0.21970989663224572 1103 86-87 0.13878263098942512 1103 88-89 0.14050944692096579 1103 90-91 0.13720578634116976 1103 92-93 0.09846004421284249 1103 94-95 0.12963903792208242 1103 96-97 0.0033440827680664142 1103 98-99 0.08473133249426468 1103 100 -0.06301048682916033 1104 1 0.19896168423512606 1104 2 0.3226819836275574 1104 3 0.27823639976468684 1104 4 0.3002036582811485 1104 5 0.2819686192327495 1104 6 0.3209189845145204 1104 7 0.2781602709858291 1104 8 0.23991042087887138 1104 9 0.42322405912346284 1104 10-11 0.353735403694337 1104 12-13 0.37148571441014866 1104 14-15 0.45361996192154663 1104 16-17 0.45073484886531645 1104 18-19 0.4136155398672372 1104 20-21 0.41608607787044605 1104 22-23 0.39710387680779036 1104 24-25 0.4158699966342283 1104 26-27 0.4336881699500239 1104 28-29 0.5059641338106218 1104 30-31 0.46907947711687825 1104 32-33 0.5096352970592193 1104 34-35 0.4769926698561875 1104 36-37 0.4905236070660237 1104 38-39 0.47511514770440044 1104 40-41 0.5257618675975735 1104 42-43 0.45626335545547647 1104 44-45 0.43543997090218767 1104 46-47 0.4472676693986273 1104 48-49 0.4509106105005003 1104 50-51 0.4486708332331659 1104 52-53 0.40474650815252033 1104 54-55 0.4595896376551565 1104 56-57 0.40281283836939963 1104 58-59 0.5408312400778215 1104 60-61 0.5093967428890309 1104 62-63 0.5094097454608004 1104 64-65 0.4529434174337723 1104 66-67 0.29516964554411373 1104 68-69 0.37606705226055936 1104 70-71 0.434899809036267 1104 72-73 0.38313526252078134 1104 74-75 0.3619397512980953 1104 76-77 0.35531680377523145 1104 78-79 0.3983648708178933 1104 80-81 0.3560171931642202 1104 82-83 0.3385249073878782 1104 84-85 0.30026085012964643 1104 86-87 0.27914291986184026 1104 88-89 0.199872414927043 1104 90-91 0.25602315679355314 1104 92-93 0.3785587051107342 1104 94-95 0.2611405262005846 1104 96-97 0.1862996342443779 1104 98-99 0.23711664442860325 1104 100 0.1739864204328505 1105 1 0.037108938969012684 1105 2 0.08179939419436977 1105 3 0.12406483377707289 1105 4 0.05593174782745791 1105 5 -0.06811407174517115 1105 6 0.03357150077389548 1105 7 -0.06196508789938804 1105 8 0.09454658588693832 1105 9 -0.04884614695073708 1105 10-11 -0.031661698881777056 1105 12-13 0.028344338861394647 1105 14-15 0.04543764451352672 1105 16-17 0.07388423327692806 1105 18-19 0.07494723106502477 1105 20-21 0.08931273612462576 1105 22-23 0.00143742576436523 1105 24-25 0.07408348912512253 1105 26-27 0.0839326845693833 1105 28-29 0.08733508330698925 1105 30-31 0.1462844370884966 1105 32-33 0.07963599810026523 1105 34-35 0.13599212495609692 1105 36-37 0.09305702070042088 1105 38-39 0.07896763379776672 1105 40-41 0.14582444082184054 1105 42-43 0.16296105912117298 1105 44-45 0.06049071671426276 1105 46-47 0.028264093027111414 1105 48-49 0.06654722481214748 1105 50-51 0.005248244628482723 1105 52-53 -0.04465585435580266 1105 54-55 0.0205619922077247 1105 56-57 -0.02545218063838206 1105 58-59 -0.008955739040715116 1105 60-61 0.02164244290507611 1105 62-63 0.04394451444122183 1105 64-65 0.004724274127170247 1105 66-67 -0.024632108545560527 1105 68-69 0.024124956126591712 1105 70-71 -0.024860541144363424 1105 72-73 -0.038257164323994175 1105 74-75 0.048122179173184065 1105 76-77 -0.08691056295527488 1105 78-79 -0.06767627604276782 1105 80-81 -0.08769962024307887 1105 82-83 -0.06314234879057778 1105 84-85 -0.08014351247003049 1105 86-87 -0.018565578773788616 1105 88-89 -0.037797760131404345 1105 90-91 -0.01470471500515913 1105 92-93 0.008309824917589026 1105 94-95 -0.0323824387356737 1105 96-97 -0.08441766882947377 1105 98-99 -0.13548534376628396 1105 100 -0.21016474101159233 1106 1 0.2693488993413595 1106 2 0.3078557999198068 1106 3 0.2828629133789349 1106 4 0.24293207785379423 1106 5 0.25761457313240754 1106 6 0.2450654179415679 1106 7 0.21542296490238044 1106 8 0.19226683254335342 1106 9 0.3336819282487298 1106 10-11 0.25171121924291384 1106 12-13 0.3087597250687466 1106 14-15 0.4511039946243258 1106 16-17 0.39010808909888794 1106 18-19 0.2999077840397604 1106 20-21 0.2482490410420155 1106 22-23 0.32192039695654984 1106 24-25 0.32393179334695077 1106 26-27 0.37518322109178825 1106 28-29 0.36331164592657217 1106 30-31 0.2896397027841857 1106 32-33 0.23129825319040975 1106 34-35 0.2953052966806169 1106 36-37 0.3567587773099845 1106 38-39 0.2549299912022036 1106 40-41 0.3161656353947109 1106 42-43 0.26579763240691534 1106 44-45 0.15346201299524864 1106 46-47 0.22026456694247543 1106 48-49 0.29382515658181063 1106 50-51 0.24206326394941158 1106 52-53 0.2383551981587999 1106 54-55 0.2633528455186678 1106 56-57 0.21866811549137566 1106 58-59 0.21802002576011148 1106 60-61 0.1816540887323157 1106 62-63 0.2512925905836241 1106 64-65 0.25732560415166716 1106 66-67 0.1659789177628639 1106 68-69 0.31734677239804654 1106 70-71 0.2833243568750561 1106 72-73 0.3191425281820841 1106 74-75 0.27423108908823934 1106 76-77 0.2688693064179759 1106 78-79 0.226740584567942 1106 80-81 0.24726945586074578 1106 82-83 0.3131815068477053 1106 84-85 0.2592348440478105 1106 86-87 0.21415457297709395 1106 88-89 0.1736154873338478 1106 90-91 0.19657474627430105 1106 92-93 0.23593615183469296 1106 94-95 0.19713235519991912 1106 96-97 0.2739082710567544 1106 98-99 0.2606593792105869 1106 100 0.25720765543881896 1107 1 0.03429175726759581 1107 2 0.03720312153309635 1107 3 0.15340561975716227 1107 4 0.025372748553429858 1107 5 0.07670872482498226 1107 6 0.06480773647653848 1107 7 0.014441193036965672 1107 8 0.011363676207125195 1107 9 0.12557381070929097 1107 10-11 0.0827340935203793 1107 12-13 0.00793682935520934 1107 14-15 0.05478147653452936 1107 16-17 0.07562997008884054 1107 18-19 0.11187210046602303 1107 20-21 0.10475865112992722 1107 22-23 0.13523279273614008 1107 24-25 0.0781849832081889 1107 26-27 0.11607476207081646 1107 28-29 0.18439062786295324 1107 30-31 0.08930544969402376 1107 32-33 0.13238017635458021 1107 34-35 0.12592572126429502 1107 36-37 0.14895684656841723 1107 38-39 0.09987743544974137 1107 40-41 0.08190606456471983 1107 42-43 0.10019180179671139 1107 44-45 0.06415327630243439 1107 46-47 -0.019987581026207124 1107 48-49 0.04886180976369303 1107 50-51 0.03229729096383949 1107 52-53 0.03137028067519054 1107 54-55 0.012686707275975095 1107 56-57 0.015914577917186534 1107 58-59 -0.08189288398656913 1107 60-61 -0.04025392062975186 1107 62-63 0.03257232377936958 1107 64-65 0.020934440796374076 1107 66-67 6.461726750899288E-4 1107 68-69 0.0937616649809172 1107 70-71 0.035256020687732814 1107 72-73 -0.02452393658674623 1107 74-75 -0.06685890998950583 1107 76-77 -0.05708418728704601 1107 78-79 -0.029576599430782835 1107 80-81 -0.029491504574757244 1107 82-83 0.028485861609617302 1107 84-85 0.04420252793062218 1107 86-87 -0.08750108901102038 1107 88-89 -0.03456596149501934 1107 90-91 -0.027968852442519676 1107 92-93 0.07864950924621184 1107 94-95 0.06431996814431074 1107 96-97 0.04267706811951655 1107 98-99 -0.06333037135834019 1107 100 0.021742131364369754 1108 1 0.1367003365632833 1108 2 0.20535296936653324 1108 3 0.12941740662736123 1108 4 0.12391678024272323 1108 5 0.16018391926003517 1108 6 0.09765107737794665 1108 7 0.016231444715629095 1108 8 0.07765564277878667 1108 9 0.08968134174644149 1108 10-11 0.05397847784963972 1108 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2 -0.3898778420807858 2215 3 -0.516327391313677 2215 4 -0.4848157130817867 2215 5 -0.5420632073760459 2215 6 -0.42917128291919937 2215 7 -0.40424071723911936 2215 8 -0.3306571728087633 2215 9 -0.4327898492276603 2215 10-11 -0.38952680751162205 2215 12-13 -0.5059145106207623 2215 14-15 -0.6586255978883742 2215 16-17 -0.6759539861928658 2215 18-19 -0.6352129269062914 2215 20-21 -0.6881697512518201 2215 22-23 -0.6320480303878071 2215 24-25 -0.5788233552370983 2215 26-27 -0.5065462460121921 2215 28-29 -0.5137857895663132 2215 30-31 -0.47081650176147605 2215 32-33 -0.5712492630596202 2215 34-35 -0.6174026517035855 2215 36-37 -0.4646612336733966 2215 38-39 -0.5931530253224153 2215 40-41 -0.5475211583595296 2215 42-43 -0.5517503844666223 2215 44-45 -0.5193886181443403 2215 46-47 -0.47558389887403507 2215 48-49 -0.39530838043955185 2215 50-51 -0.4947630055357095 2215 52-53 -0.5124768987861898 2215 54-55 -0.41768272706819687 2215 56-57 -0.4142984984834044 2215 58-59 -0.3086353945765268 2215 60-61 -0.38799932809894244 2215 62-63 -0.3925623317590521 2215 64-65 -0.4328496343018813 2215 66-67 -0.3599892830367182 2215 68-69 -0.3587756103271005 2215 70-71 -0.3864876413004481 2215 72-73 -0.34854814110602916 2215 74-75 -0.2792875861911668 2215 76-77 -0.380555178824757 2215 78-79 -0.4006160120822315 2215 80-81 -0.4107485605172272 2215 82-83 -0.3015563434244015 2215 84-85 -0.31303597405190686 2215 86-87 -0.3005238092909224 2215 88-89 -0.3411493662666416 2215 90-91 -0.3775311856591017 2215 92-93 -0.4222942502721736 2215 94-95 -0.45644022560021114 2215 96-97 -0.3951098090369385 2215 98-99 -0.3755901113165052 2215 100 -0.3405246717929771 2216 1 -0.24150182300957823 2216 2 -0.33702566407103873 2216 3 -0.32561191550229296 2216 4 -0.28538513822822864 2216 5 -0.2581724500476099 2216 6 -0.274866885907727 2216 7 -0.22766262958831618 2216 8 -0.21486343450909118 2216 9 -0.2806714786684452 2216 10-11 -0.33048158914963466 2216 12-13 -0.3379075081959826 2216 14-15 -0.6068321349935957 2216 16-17 -0.559855389961001 2216 18-19 -0.564055470844373 2216 20-21 -0.5873064652441471 2216 22-23 -0.617999638813032 2216 24-25 -0.7429829447307768 2216 26-27 -0.6802946119908881 2216 28-29 -0.6266959046084253 2216 30-31 -0.6207142608771719 2216 32-33 -0.6122355696343718 2216 34-35 -0.5867145761304329 2216 36-37 -0.46854450661846414 2216 38-39 -0.5403219170631033 2216 40-41 -0.5877059537773448 2216 42-43 -0.5217471873471524 2216 44-45 -0.5062404244849645 2216 46-47 -0.42106481991220335 2216 48-49 -0.4520244510490343 2216 50-51 -0.4219582519960099 2216 52-53 -0.3930207852275629 2216 54-55 -0.3406360048169148 2216 56-57 -0.38199283346183677 2216 58-59 -0.48372361817981613 2216 60-61 -0.4659516033610487 2216 62-63 -0.5812632716739188 2216 64-65 -0.5375056666415574 2216 66-67 -0.54550454681182 2216 68-69 -0.5104478185612678 2216 70-71 -0.5993833167102665 2216 72-73 -0.5445110685454466 2216 74-75 -0.6131340205423257 2216 76-77 -0.5281480474951792 2216 78-79 -0.5734817435834856 2216 80-81 -0.5388908938534271 2216 82-83 -0.4944131242661669 2216 84-85 -0.36564213642796517 2216 86-87 -0.41779552205125015 2216 88-89 -0.4237153431141394 2216 90-91 -0.36406799763691566 2216 92-93 -0.45926525529927176 2216 94-95 -0.4910779709265327 2216 96-97 -0.437679151944419 2216 98-99 -0.4307243887404084 2216 100 -0.5025153520144094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 68.0 1 50.0 2 28.0 3 27.0 4 21.0 5 28.0 6 105.0 7 569.0 8 1600.0 9 1887.0 10 1753.0 11 2214.0 12 2870.0 13 3699.0 14 4661.0 15 5197.0 16 5580.0 17 6393.0 18 6968.0 19 7745.0 20 8470.0 21 9688.0 22 11227.0 23 13329.0 24 17098.0 25 21866.0 26 24109.0 27 22516.0 28 22493.0 29 26988.0 30 31117.0 31 34032.0 32 41880.0 33 56621.0 34 88486.0 35 137077.0 36 263925.0 37 523111.0 38 571415.0 39 191093.0 40 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.944173287651005 21.886491345955047 13.44902826594205 23.7203071004519 2 14.781354878348035 21.670092269883412 40.491229656252706 23.057323195515842 3 17.185793307551513 27.889753203046585 31.170754118202755 23.75369937119915 4 11.964682477632497 18.402491894562196 41.105097857968836 28.52772776983647 5 12.361573404380941 39.65015572415422 35.79243114187873 12.195839729586114 6 28.002584951926536 40.05176361620199 19.09829275087964 12.847358680991839 7 25.00590375404961 33.736264547218966 24.412807087456738 16.845024611274695 8 24.757026972300775 35.534877370748404 21.644121715522648 18.063973941428173 9 26.07454878983101 15.405836615539739 22.138714752700988 36.38089984192826 10-11 23.795843286772993 27.164783758015986 29.28700546261066 19.75236749260036 12-13 24.282519825519742 25.41734719716758 28.52022034658971 21.779912630722972 14-15 22.184408887712156 26.496251429051142 26.190872108081354 25.12846757515535 16-17 21.51514368767776 29.06233308086935 27.203770723341602 22.21875250811129 18-19 21.101639994557182 29.33380842667995 28.582445992725997 20.982105586036866 20-21 22.707960968904736 28.389932726480215 27.48991589080334 21.412190413811715 22-23 23.058133313654224 28.040399055402837 27.15110325996716 21.750364370975774 24-25 22.456120283051504 28.258150276326678 27.662094584808905 21.623634855812917 26-27 22.276366890215215 28.687743881626893 27.058245620508657 21.977643607649235 28-29 21.763773077359488 28.830428858588007 27.410248178764725 21.995549885287776 30-31 22.22566629558314 28.4550032968546 27.565417598123226 21.753912809439036 32-33 22.52795799882792 28.817801419273927 26.629340901810224 22.024899680087938 34-35 22.597710093913225 28.589022907133177 27.49577875972915 21.31748823922445 36-37 22.35702749867722 28.195843022598726 27.41446652492392 22.032662953800138 38-39 22.39052389151512 28.237345564042272 27.75516747068124 21.61696307376137 40-41 22.237055639461236 27.616042786669116 28.014722909818257 22.132178664051395 42-43 22.050802505387175 27.94645258526634 27.566686303917287 22.4360586054292 44-45 21.98838605680932 28.581008107246515 27.53633451699532 21.89427131894885 46-47 22.078352878377515 28.021219696569506 27.32842156303914 22.572005862013842 48-49 22.60654249242904 27.729052748522253 27.628867834344952 22.03553692470376 50-51 22.274184875446114 27.793832041008077 27.26519861470176 22.666784468844053 52-53 21.839832157443514 28.248137549697063 27.24002108412543 22.672009208733996 54-55 21.917474821063333 28.162252518816437 27.21514431894258 22.705128341177648 56-57 22.670602690590407 27.789548527659136 27.062433010924497 22.47741577082596 58-59 21.988229193003956 27.950729815871213 27.838867470457636 22.222173520667194 60-61 22.176616482153356 28.310391149451515 27.253579628937118 22.25941273945801 62-63 22.511262009426687 27.805421451059857 26.944945798407726 22.738370741105733 64-65 21.624588794921127 27.906810432728463 27.389487914146375 23.07911285820403 66-67 22.479817733265108 27.52578813153066 27.2058385889277 22.78855554627653 68-69 21.728929487966234 27.681724601558237 27.472456741465717 23.11688916900981 70-71 21.97209170987301 27.41152293185156 27.378855753645183 23.23752960463025 72-73 22.376889814931015 27.555979308002925 27.60417051150499 22.462960365561074 74-75 22.2684512107176 27.9597536347366 27.360450614598193 22.411344539947613 76-77 22.06784815312862 27.755450250524316 27.23029389054721 22.94640770579986 78-79 21.862958445614478 27.931134795277856 27.25148446419603 22.95442229491163 80-81 21.81008114806026 27.846468811266504 27.748488538361343 22.594961502311893 82-83 22.36371060578447 27.09680693748832 28.326093325735748 22.213389130991462 84-85 22.51091099136325 27.772870519826643 27.15976957905569 22.556448909754412 86-87 21.59449677155927 28.034030694985596 27.67822169318394 22.693250840271194 88-89 22.306879424885683 27.138875925922935 27.506857556368523 23.04738709282286 90-91 22.288275157921497 27.121458430365404 27.78195702929728 22.808309382415818 92-93 22.560514232563634 27.144365846276504 27.78664903590622 22.508470885253647 94-95 21.444397228501018 27.369498285230748 28.119724252738486 23.06638023352975 96-97 21.824269847989047 27.31301983789651 27.76899502524288 23.09371528887156 98-99 21.468349069236822 27.725478553473355 27.535812646439172 23.27035973085065 100 22.124543780677016 27.170994992948238 27.20444300297618 23.500018223398566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 847.0 1 633.5 2 441.5 3 414.5 4 335.0 5 272.0 6 235.0 7 244.5 8 267.5 9 255.0 10 211.0 11 186.5 12 205.0 13 223.0 14 232.0 15 291.0 16 343.0 17 408.5 18 541.0 19 690.5 20 933.0 21 1293.0 22 1823.5 23 2680.5 24 3929.5 25 5713.5 26 8262.5 27 11410.5 28 14720.5 29 19200.5 30 25557.0 31 31535.5 32 39041.5 33 46653.0 34 52669.0 35 58558.0 36 65201.5 37 74000.0 38 77411.5 39 76358.0 40 74132.0 41 70253.5 42 67816.5 43 69715.0 44 76198.5 45 87371.5 46 94287.0 47 97046.5 48 96868.5 49 89532.5 50 83456.0 51 83312.5 52 82990.0 53 79337.0 54 73142.0 55 63225.0 56 56487.5 57 45783.5 58 34685.0 59 31268.5 60 25580.0 61 18265.0 62 13093.0 63 10272.0 64 7457.5 65 4066.5 66 2466.5 67 1741.5 68 1255.5 69 820.5 70 596.0 71 433.0 72 309.5 73 250.5 74 185.0 75 136.0 76 124.5 77 105.0 78 64.0 79 38.5 80 31.0 81 27.5 82 20.0 83 16.0 84 19.0 85 17.5 86 14.5 87 11.0 88 10.0 89 10.0 90 7.0 91 4.0 92 3.5 93 4.5 94 6.5 95 4.5 96 1.5 97 2.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06212540846187727 2 0.00954711176808359 3 0.01194542003832681 4 0.009500990455194298 5 0.01194542003832681 6 0.012129905289883981 7 0.0038280689698112946 8 0.007194924810729663 9 0.0088552920747442 10-11 0.008486321571629858 12-13 0.007194924810729663 14-15 0.010861569185428433 16-17 0.01056178065164803 18-19 0.009224262577858543 20-21 0.008301836320072687 22-23 0.003459098466696953 24-25 0.005027223104932905 26-27 0.006226377240054516 28-29 0.007425531375176125 30-31 0.01012362817919975 32-33 0.011783995443214287 34-35 0.01335212008145024 36-37 0.019278708787724353 38-39 0.019716861260172632 40-41 0.01923258747483506 42-43 0.025182236837553815 44-45 0.02896418449447582 46-47 0.03138555342116369 48-49 0.03242328296117277 50-51 0.0331842846238461 52-53 0.031708402611388735 54-55 0.03726602081454851 56-57 0.0398488143363489 58-59 0.03685092899854487 60-61 0.03811926510300043 62-63 0.03371467972207297 64-65 0.036205230618094775 66-67 0.037911719194998604 68-69 0.040448391403909705 70-71 0.04067899796835617 72-73 0.036297473243873356 74-75 0.038926388078563044 76-77 0.03876496348345052 78-79 0.03830375035455759 80-81 0.04252385048392788 82-83 0.038741902827005875 84-85 0.03553647158120003 86-87 0.0341297715380766 88-89 0.03207737311450307 90-91 0.03419895350741054 92-93 0.03555953223764468 94-95 0.03911087333012021 96-97 0.03470628794919276 98-99 0.03226185836606025 100 0.030071096003818847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2168195.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.36061044630625 #Duplication Level Percentage of deduplicated Percentage of total 1 69.08227883593715 36.86272569707549 2 14.98026463878659 15.987121315457353 3 6.449316001634847 10.324183164250995 4 3.273786113083145 6.987649018590271 5 1.8974096967345668 5.062346984224864 6 1.1950087745932583 3.8259838620593185 7 0.763976327146039 2.8536370228127725 8 0.5247947167735083 2.2402693156824633 9 0.37280560581732725 1.7903821233795838 >10 1.4169971447664638 11.862701874755098 >50 0.03290404030413163 1.189619642017987 >100 0.010175459944063443 0.7940865084055042 >500 0.0 0.0 >1k 2.826444788703164E-4 0.21929347128834567 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3060656444646353E-4 2 0.0 0.0 0.0 0.0 2.3060656444646353E-4 3 0.0 0.0 0.0 0.0 2.3060656444646353E-4 4 0.0 0.0 0.0 0.0 2.3060656444646353E-4 5 0.0 0.0 0.0 0.0 2.3060656444646353E-4 6 0.0 0.0 0.0 0.0 5.073344417822198E-4 7 0.0 0.0 0.0 0.0 5.073344417822198E-4 8 0.0 0.0 0.0 0.0 5.073344417822198E-4 9 0.0 0.0 0.0 4.612131288929271E-5 5.073344417822198E-4 10-11 0.0 0.0 0.0 4.612131288929271E-5 5.073344417822198E-4 12-13 0.0 0.0 0.0 4.612131288929271E-5 0.0011991541351216103 14-15 0.0 0.0 0.0 4.612131288929271E-5 0.001637306607569891 16-17 0.0 0.0 0.0 4.612131288929271E-5 0.0021215803929074645 18-19 0.0 0.0 0.0 4.612131288929271E-5 0.002167701705796757 20-21 0.0 0.0 0.0 9.224262577858542E-5 0.0024444295831325133 22-23 0.0 0.0 0.0 2.9978853378040263E-4 0.002490550896021806 24-25 0.0 0.0 0.0 0.0010377295400090859 0.002490550896021806 26-27 0.0 0.0 0.0 0.0025597328653557453 0.002675036147578977 28-29 0.0 0.0 0.0 0.007310228092952894 0.002813400086246855 30-31 0.0 0.0 0.0 0.018563828437940313 0.002813400086246855 32-33 0.0 0.0 0.0 0.04690537520841068 0.002882582055580794 34-35 0.0 0.0 0.0 0.09334953728792844 0.0030209459942486727 36-37 0.0 0.0 0.0 0.1626698705605354 0.003044006650693319 38-39 0.0 0.0 0.0 0.2517762470626489 0.003136249276471904 40-41 0.0 0.0 0.0 0.34662472701947933 0.003182370589361197 42-43 0.0 0.0 0.0 0.4446094562527817 0.0032746132151397823 44-45 0.0 0.0 0.0 0.5617345303351404 0.0034821591231415996 46-47 0.0 0.0 0.0 0.6956708229656465 0.0036205230618094773 48-49 0.0 0.0 0.0 0.8365714338424357 0.0038741902827005873 50-51 0.0 0.0 0.0 0.9705769084422757 0.004012554221368466 52-53 0.0 0.0 0.0 1.1088947257972646 0.0044276460373721 54-55 0.0 0.0 0.0 1.2564598663865567 0.004496828006706039 56-57 0.0 0.0 0.0 1.4312596422369759 0.0046121312889292705 58-59 0.0 0.0 0.0 1.6208412988684135 0.004704373914707856 60-61 0.0 0.0 0.0 1.8151273294145591 0.004750495227597149 62-63 0.0 0.0 0.0 2.0063693533100113 0.004981101792043612 64-65 0.0 0.0 0.0 2.206697275844654 0.0050733444178221974 66-67 0.0 0.0 0.0 2.4314464335541777 0.00511946573071149 68-69 0.0 0.0 0.0 2.671899898302505 0.005188647700045429 70-71 0.0 0.0 0.0 2.9225231125429216 0.0053039509822686605 72-73 0.0 0.0 0.0 3.1756599383358046 0.00562680017249371 74-75 0.0 0.0 0.0 3.4336395019820634 0.005972710019163406 76-77 0.0 0.0 0.0 3.7178851533187744 0.0060418919884973445 78-79 0.0 0.0 0.0 4.018895901890743 0.006157195270720577 80-81 0.0 0.0 0.0 4.340568998637115 0.0062955592093884545 82-83 0.0 0.0 0.0 4.664571221684397 0.006664529712502796 84-85 0.0 0.0 0.0 4.985414134798761 0.006687590368947442 86-87 4.612131288929271E-5 0.0 0.0 5.327749579719536 0.006918196933393905 88 4.612131288929271E-5 0.0 0.0 5.597190289618784 0.007148803497840369 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2560 0.0 38.01642 1 GTATCAA 4060 0.0 34.045685 1 CAACGCA 4055 0.0 31.867075 5 ATCAACG 4115 0.0 31.28968 3 TATCAAC 5005 0.0 27.13402 2 CGTTAAC 420 0.0 26.865984 1 AACGCAG 5430 0.0 26.393095 6 GTGATCG 1150 0.0 26.151255 8 ACGCAGA 4960 0.0 26.051414 7 CGCAGAG 4965 0.0 26.026981 8 GTACTAG 1195 0.0 25.966705 1 GCATCGT 1080 0.0 25.670753 8 TATTCGC 280 0.0 25.1735 9 TCGCCGG 115 1.3768378E-4 24.524153 94 TGATCGC 1265 0.0 24.5168 9 GTACATG 8375 0.0 24.419977 1 TACATGG 8345 0.0 24.38271 2 ACGTTAA 330 0.0 24.207241 8 GTCCTAC 1210 0.0 24.090572 1 ACATGGG 8410 0.0 23.803123 3 >>END_MODULE