FastQCFastQC Report
Mon 16 Jan 2017
ERR1146548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168195
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71360.32912168877799275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69770.3217884000285952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.16737424447524324No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT26570.12254432834685072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.060.565821
GTATCAA86450.042.734111
GTGGTAT9650.037.5520321
ATCAACG108550.033.392853
CAACGCA110150.032.9077955
TATCAAC113300.032.3969882
AACGCAG124700.029.811156
TCGACCG404.7855725E-429.3723580-81
ACGCAGA128450.028.2084317
TCAACGC130300.028.1435664
CGCAGAG129200.028.0083078
GTGATCG11150.026.5536548
GCAGAGT138450.025.9673189
TGATCGC12050.025.3504079
TATTCGC1301.0891699E-525.3054089
CTATTGA19650.025.1122369
GTACATG87050.024.6527881
GGGCTAT20750.024.4610086
TACATGG85850.024.3926852
GCTATTG20050.024.3768528
Produced by FastQC (version 0.11.5)