FastQCFastQC Report
Mon 16 Jan 2017
ERR1146547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723825
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1887246.928638954411535No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC473771.739355501913669No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT437581.6064908722109534No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC358441.3159435719989354No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA331301.2163042779914275No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA304051.116261140124641No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG294851.0824851082576892No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC255400.937652015089075No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG163520.6003322533569522No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA84400.30985837930116655No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG78600.2885647939937404No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC53260.19553385404715795No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51690.1897699007829064No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC30070.11039622589556965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5600.033.583321
GAGTACG24150.028.8135151
GTACGCA23800.028.631453
AGTACGC24950.027.123412
TACGCAG25500.026.3540974
TAGTACT9550.024.6047524
CTAGTAC9800.024.456623
CGCAGTG27950.023.7064046
GCATCGT7000.023.4966728
TAGGCAT10000.021.6173365
GTACCCG2406.679329E-921.54931
CATGGGG36700.021.000524
CCTAGTA11000.020.9341682
CAATGCG4050.020.30539518-19
TGGGGGG24000.019.9718066
TACCTGG19350.019.6723562
CTAGGCA11300.019.5466234
TACTAGG9500.019.2927132
ACGCAGT35100.018.8780145
GTATAAC3255.6024874E-1018.806661
Produced by FastQC (version 0.11.5)