##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146547_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2723825
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21123053059576	31.0	31.0	34.0	28.0	34.0
2	31.3743382926583	31.0	31.0	34.0	28.0	34.0
3	31.578297063871577	33.0	31.0	34.0	28.0	34.0
4	35.050599065652165	37.0	35.0	37.0	32.0	37.0
5	35.14487457894688	37.0	35.0	37.0	32.0	37.0
6	35.280830082696205	37.0	35.0	37.0	32.0	37.0
7	35.24255486310611	37.0	35.0	37.0	32.0	37.0
8	35.21947885785614	37.0	35.0	37.0	32.0	37.0
9	36.85776729415436	39.0	37.0	39.0	33.0	39.0
10-11	36.832577533431845	39.0	37.0	39.0	33.0	39.0
12-13	36.82553669931071	39.0	37.0	39.0	33.0	39.0
14-15	37.99633878828486	40.0	38.0	41.0	33.0	41.0
16-17	38.13747138674474	40.0	38.0	41.0	33.0	41.0
18-19	38.219885271630886	40.0	38.0	41.0	33.0	41.0
20-21	38.23877066257928	40.0	38.0	41.0	33.0	41.0
22-23	38.18431011537085	40.0	38.0	41.0	33.0	41.0
24-25	38.122537975090175	40.0	38.0	41.0	33.0	41.0
26-27	37.96314319018292	40.0	38.0	41.0	32.5	41.0
28-29	37.95589621212817	40.0	38.0	41.0	32.5	41.0
30-31	38.01769093095187	40.0	38.0	41.0	32.5	41.0
32-33	37.88830082696208	40.0	38.0	41.0	32.0	41.0
34-35	37.583650748487884	40.0	37.0	41.0	31.0	41.0
36-37	37.39149743467367	40.0	37.0	41.0	30.5	41.0
38-39	37.26710086000385	40.0	37.0	41.0	30.0	41.0
40-41	37.39807696896827	40.0	37.0	41.0	31.0	41.0
42-43	37.18913917010087	40.0	37.0	41.0	30.0	41.0
44-45	36.93241930006516	39.5	36.0	41.0	30.0	41.0
46-47	36.62102080713702	39.0	35.5	41.0	29.5	41.0
48-49	36.348919993024516	39.0	35.0	40.0	28.5	41.0
50-51	36.05125457766193	39.0	35.0	40.0	28.0	41.0
52-53	36.09970464328656	39.0	35.0	40.0	28.0	41.0
54-55	35.842248492469224	38.5	34.5	40.0	27.5	41.0
56-57	35.20879333290502	38.0	33.5	40.0	26.5	41.0
58-59	34.86623112718328	38.0	33.0	40.0	26.0	41.0
60-61	34.30024414196947	37.0	32.0	40.0	24.0	41.0
62-63	34.387549860949214	37.0	32.5	40.0	25.5	41.0
64-65	34.18160069020587	36.0	32.5	39.0	25.5	41.0
66-67	33.69476948776078	36.0	32.0	39.0	24.5	40.0
68-69	33.033490404119206	35.0	31.0	38.5	22.5	40.0
70-71	32.37289913999614	35.0	30.0	37.0	21.5	39.5
72-73	32.134127743155304	35.0	30.5	37.0	22.0	39.0
74-75	31.99432562664635	34.0	30.0	36.0	22.5	39.0
76-77	31.788393711049718	34.0	30.0	36.0	22.0	37.5
78-79	31.345948987177955	34.0	29.5	35.0	21.0	37.0
80-81	30.719034996741712	34.0	29.0	35.0	20.0	37.0
82-83	30.364514607215956	34.0	28.5	35.0	19.0	36.0
84-85	30.361740199902712	34.0	29.0	35.0	20.0	36.0
86-87	30.630537020550143	34.0	29.0	35.0	20.0	36.0
88-89	30.395470891118187	34.0	29.0	35.0	20.0	35.0
90-91	30.04388387653392	34.0	29.0	35.0	18.5	35.0
92-93	29.582825071361043	34.0	27.0	35.0	18.0	35.0
94-95	29.563840738667132	34.0	27.5	35.0	17.5	35.0
96-97	29.59600781988564	34.0	27.5	35.0	18.0	35.0
98-99	29.77477958385726	34.0	29.0	35.0	18.0	35.0
100	28.52113076280598	32.0	25.0	35.0	11.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2562561695261536
1101	2	0.2891293007249871
1101	3	0.4529587018611494
1101	4	0.3439404020946384
1101	5	0.3145803734582415
1101	6	0.34584254135430825
1101	7	0.3068543815006777
1101	8	0.3927348707093614
1101	9	0.5646207309468281
1101	10-11	0.5098775084604128
1101	12-13	0.47697600426679543
1101	14-15	0.6061799014289235
1101	16-17	0.6086852171856094
1101	18-19	0.5259189721164361
1101	20-21	0.5116578157265508
1101	22-23	0.5472994973750787
1101	24-25	0.5850862545582629
1101	26-27	0.5911274074497186
1101	28-29	0.6326591796323839
1101	30-31	0.6678554902941727
1101	32-33	0.6710442893106929
1101	34-35	0.5859561446062855
1101	36-37	0.6896246496451681
1101	38-39	0.6436165632329107
1101	40-41	0.634188338081735
1101	42-43	0.684746470092584
1101	44-45	0.6591830249303712
1101	46-47	0.6680859902020728
1101	48-49	0.6015500220327468
1101	50-51	0.6716436897233393
1101	52-53	0.7295116972081033
1101	54-55	0.825375736649562
1101	56-57	0.8314206230641048
1101	58-59	0.8490387113581335
1101	60-61	0.8383482742196335
1101	62-63	0.8083479104614213
1101	64-65	0.7231804331156653
1101	66-67	0.7055978942211709
1101	68-69	0.7029942311528146
1101	70-71	0.6698298971380225
1101	72-73	0.6905713783024439
1101	74-75	0.6557751338069906
1101	76-77	0.6198018458183654
1101	78-79	0.704067768422945
1101	80-81	0.7079483233659865
1101	82-83	0.6404931300623531
1101	84-85	0.5924305793769982
1101	86-87	0.5792485451216152
1101	88-89	0.5270688299050477
1101	90-91	0.6415357833684503
1101	92-93	0.7402766267478036
1101	94-95	0.6791703556491129
1101	96-97	0.7110685971982669
1101	98-99	0.5535696788226332
1101	100	0.40500151290478925
1102	1	0.2443138700728298
1102	2	0.25445392906654973
1102	3	0.3737743198696606
1102	4	0.2875496564755906
1102	5	0.1813098408841185
1102	6	0.22543003844356235
1102	7	0.24947596278190076
1102	8	0.3136625140746574
1102	9	0.39286060557239466
1102	10-11	0.40490284850802283
1102	12-13	0.41408173153746475
1102	14-15	0.5582447210915973
1102	16-17	0.520369300393881
1102	18-19	0.4342237950209409
1102	20-21	0.46698664024727066
1102	22-23	0.44105673387543476
1102	24-25	0.48659750304929617
1102	26-27	0.4881529433581733
1102	28-29	0.5563775097116448
1102	30-31	0.5849838883844285
1102	32-33	0.5782018433581584
1102	34-35	0.612463832658392
1102	36-37	0.6788480085324125
1102	38-39	0.7120694886510393
1102	40-41	0.6787332464485161
1102	42-43	0.6950560697113204
1102	44-45	0.7304191858346059
1102	46-47	0.789149356031082
1102	48-49	0.7858662371672338
1102	50-51	0.7847037394255949
1102	52-53	0.8424802032537997
1102	54-55	0.8789341347486257
1102	56-57	0.9546416515890215
1102	58-59	0.9240979394432287
1102	60-61	0.8952608627277456
1102	62-63	0.9357940788271577
1102	64-65	0.906197088041452
1102	66-67	0.8884176238392385
1102	68-69	0.9054361269918445
1102	70-71	1.037029562735718
1102	72-73	0.837110492183335
1102	74-75	0.8694245572801691
1102	76-77	0.8917548148426917
1102	78-79	0.8949445143961228
1102	80-81	0.9100179514564886
1102	82-83	0.9244068247290933
1102	84-85	0.9365227026877001
1102	86-87	0.9573999373339888
1102	88-89	0.8799255425730763
1102	90-91	0.9109580878372228
1102	92-93	0.9452480125646012
1102	94-95	0.8767351842679361
1102	96-97	0.8731561111720723
1102	98-99	0.8361975044530361
1102	100	0.6948012738194791
1103	1	0.2233704666307048
1103	2	0.19581454336930193
1103	3	0.190405679116477
1103	4	0.17099924547636647
1103	5	0.16204044951990682
1103	6	0.16449895396881686
1103	7	0.18171121218014719
1103	8	0.2217184581792253
1103	9	0.27550940374876376
1103	10-11	0.30151420536753193
1103	12-13	0.30586994612159657
1103	14-15	0.39401509693018966
1103	16-17	0.3383807490372277
1103	18-19	0.35474067015204014
1103	20-21	0.3353436110097121
1103	22-23	0.3528357468978456
1103	24-25	0.4074561049450054
1103	26-27	0.420259701175155
1103	28-29	0.42752232769107934
1103	30-31	0.449086440600972
1103	32-33	0.4475240220191097
1103	34-35	0.4722156338582977
1103	36-37	0.5462897483924465
1103	38-39	0.533320026084823
1103	40-41	0.5320490245773115
1103	42-43	0.49163636840282265
1103	44-45	0.48782780470800446
1103	46-47	0.5389090318672487
1103	48-49	0.4947541461528928
1103	50-51	0.4825344191011567
1103	52-53	0.5023663194414851
1103	54-55	0.5758036626297098
1103	56-57	0.6775829470159422
1103	58-59	0.8056497063400911
1103	60-61	0.6380008710949099
1103	62-63	0.6125344661503291
1103	64-65	0.5765062327018171
1103	66-67	0.5802567135567855
1103	68-69	0.5756695094233493
1103	70-71	0.5413737950137332
1103	72-73	0.5573334736052189
1103	74-75	0.44328997806618986
1103	76-77	0.4564269309816993
1103	78-79	0.539926910904633
1103	80-81	0.608863816026652
1103	82-83	0.5490788597460181
1103	84-85	0.5131126664956618
1103	86-87	0.5971399642433433
1103	88-89	0.5246386427460976
1103	90-91	0.5871148307625091
1103	92-93	0.6279794104023502
1103	94-95	0.587574948457636
1103	96-97	0.4109421968810274
1103	98-99	0.44052629456355774
1103	100	0.5010446609212984
1104	1	0.08005940484791196
1104	2	0.12175434929128315
1104	3	0.25818878336836804
1104	4	0.20567892817596345
1104	5	0.21724391037493973
1104	6	0.1937653767001919
1104	7	0.22740202696461864
1104	8	0.16597887478823026
1104	9	0.2543194165359921
1104	10-11	0.236227931986555
1104	12-13	0.3419425233293296
1104	14-15	0.45390194933457906
1104	16-17	0.4178893541222237
1104	18-19	0.34149896939531743
1104	20-21	0.40459378676490587
1104	22-23	0.3636049734089255
1104	24-25	0.4454679400506478
1104	26-27	0.3948727629690154
1104	28-29	0.4134015741856629
1104	30-31	0.386726283611452
1104	32-33	0.4227275935860746
1104	34-35	0.45809557230246867
1104	36-37	0.4687786633601405
1104	38-39	0.5045264816976456
1104	40-41	0.5099902254696431
1104	42-43	0.5495644499310899
1104	44-45	0.5576009559571133
1104	46-47	0.6361965895066461
1104	48-49	0.6026509515600722
1104	50-51	0.5796668416443254
1104	52-53	0.6847777914119817
1104	54-55	0.6497974295478741
1104	56-57	0.7309218805278874
1104	58-59	0.8077125464333079
1104	60-61	0.7824193289883894
1104	62-63	0.7048244871302387
1104	64-65	0.7426339167742526
1104	66-67	0.708391245162268
1104	68-69	0.6455344131869936
1104	70-71	0.6867427148527554
1104	72-73	0.6083221311676752
1104	74-75	0.639842408469903
1104	76-77	0.6463702741499375
1104	78-79	0.7187495171742526
1104	80-81	0.6116240029083961
1104	82-83	0.6316501720699179
1104	84-85	0.6866389522763754
1104	86-87	0.5856473736394641
1104	88-89	0.5169016776923208
1104	90-91	0.6245760123909676
1104	92-93	0.6989558619741807
1104	94-95	0.5075320199055824
1104	96-97	0.506760468778463
1104	98-99	0.43875850177201414
1104	100	0.5129777466289411
1105	1	0.18359473290541928
1105	2	0.24181236234421988
1105	3	0.2686257729953816
1105	4	0.2944215583112211
1105	5	0.2796574425501106
1105	6	0.2749726157202801
1105	7	0.2783021751501309
1105	8	0.2059126642386815
1105	9	0.36739978353161007
1105	10-11	0.3478529280454552
1105	12-13	0.36386909267699963
1105	14-15	0.3898076150658909
1105	16-17	0.4444916494212805
1105	18-19	0.40129939188168606
1105	20-21	0.44696254799704604
1105	22-23	0.4232712407103705
1105	24-25	0.4808893229052771
1105	26-27	0.4633339982687019
1105	28-29	0.4626100428302422
1105	30-31	0.40092371716443154
1105	32-33	0.40804177840809785
1105	34-35	0.4564579647103244
1105	36-37	0.4885371524984663
1105	38-39	0.42211074250415237
1105	40-41	0.4980560892020307
1105	42-43	0.47005204333525086
1105	44-45	0.4937599558625294
1105	46-47	0.4300514622878069
1105	48-49	0.4141078989384468
1105	50-51	0.45975859054869517
1105	52-53	0.45848006464085245
1105	54-55	0.5296894432944441
1105	56-57	0.5682780302267858
1105	58-59	0.6051788894039518
1105	60-61	0.5504260761586792
1105	62-63	0.5314486949683044
1105	64-65	0.5350684156234422
1105	66-67	0.5383367002809791
1105	68-69	0.4789633314327375
1105	70-71	0.5547716898503907
1105	72-73	0.5255291494859264
1105	74-75	0.427276749674931
1105	76-77	0.4090109715677315
1105	78-79	0.503890923990781
1105	80-81	0.5846072610980073
1105	82-83	0.6059647352379329
1105	84-85	0.6017860342828705
1105	86-87	0.5862331793587927
1105	88-89	0.558106709694087
1105	90-91	0.5606402681528628
1105	92-93	0.5476392179031784
1105	94-95	0.5393878070229015
1105	96-97	0.49970778409832306
1105	98-99	0.44355009618510266
1105	100	0.488643162651897
1106	1	0.013502749856453278
1106	2	0.06495646112327691
1106	3	0.1292450915350507
1106	4	0.10504324615668281
1106	5	0.09890472309690779
1106	6	0.11245923214166709
1106	7	0.09501177923613824
1106	8	0.08822492674576665
1106	9	0.0897157649490552
1106	10-11	0.11625094742577602
1106	12-13	0.09784730706018507
1106	14-15	0.1516754078026139
1106	16-17	0.11105831538814925
1106	18-19	0.15130308080082955
1106	20-21	0.1461889846511113
1106	22-23	0.11106757638402343
1106	24-25	0.11802270282495186
1106	26-27	0.2048137047147094
1106	28-29	0.13694503267822
1106	30-31	0.09116637606538092
1106	32-33	0.13281847883487075
1106	34-35	0.2500802433826692
1106	36-37	0.22831977618972132
1106	38-39	0.16163553174090595
1106	40-41	0.21186755244330158
1106	42-43	0.301285521926026
1106	44-45	0.3068087125768102
1106	46-47	0.2572057760738673
1106	48-49	0.2635694367078756
1106	50-51	0.3053835924231407
1106	52-53	0.27053242329976257
1106	54-55	0.25616889481514704
1106	56-57	0.22954407927441167
1106	58-59	0.31237642052733605
1106	60-61	0.3142337005247384
1106	62-63	0.3340184320353501
1106	64-65	0.25082651849930926
1106	66-67	0.26458788868277594
1106	68-69	0.28635550552572653
1106	70-71	0.23765150606424612
1106	72-73	0.27034009987746543
1106	74-75	0.22761328107123546
1106	76-77	0.28322947554606515
1106	78-79	0.17970109161166903
1106	80-81	0.33601840763207136
1106	82-83	0.33465228978445083
1106	84-85	0.33772207502293483
1106	86-87	0.24734615325744258
1106	88-89	0.2521056180139496
1106	90-91	0.2692164053446646
1106	92-93	0.3546848435348693
1106	94-95	0.19141263374866924
1106	96-97	0.13127779408539553
1106	98-99	0.22784534767641418
1106	100	0.19634118713072368
1107	1	0.16445306029079987
1107	2	0.18612951218704055
1107	3	0.22688239040794755
1107	4	0.17719730281759638
1107	5	0.188313002999017
1107	6	0.17405840290003738
1107	7	0.1034870678592128
1107	8	0.16556186164486775
1107	9	0.23936837834702374
1107	10-11	0.2539931883379438
1107	12-13	0.14670497477087707
1107	14-15	0.3591170958476795
1107	16-17	0.34953363942872073
1107	18-19	0.24510749830699297
1107	20-21	0.2735716081194539
1107	22-23	0.2452560848234171
1107	24-25	0.3483175298636141
1107	26-27	0.3853401031450119
1107	28-29	0.3615091908810868
1107	30-31	0.3339697770894148
1107	32-33	0.3472736574039814
1107	34-35	0.2720357259519517
1107	36-37	0.34300327954670706
1107	38-39	0.2997271072173575
1107	40-41	0.3898255921557592
1107	42-43	0.4019141824717636
1107	44-45	0.3196561729652956
1107	46-47	0.3860380376344281
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2216	80-81	-2.7453552384853204
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2216	84-85	-2.669022558034822
2216	86-87	-2.655480728573515
2216	88-89	-2.547949329817463
2216	90-91	-2.5967267581474296
2216	92-93	-2.8592792478575504
2216	94-95	-2.605941891084857
2216	96-97	-2.4844960126105278
2216	98-99	-2.4118459359930355
2216	100	-2.4453134368658773
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	77.0
1	34.0
2	31.0
3	26.0
4	25.0
5	46.0
6	93.0
7	421.0
8	1234.0
9	1658.0
10	1905.0
11	3099.0
12	4700.0
13	6116.0
14	7177.0
15	7564.0
16	8178.0
17	9226.0
18	10270.0
19	11356.0
20	13079.0
21	15237.0
22	17445.0
23	20981.0
24	25125.0
25	30434.0
26	34874.0
27	38212.0
28	44350.0
29	53786.0
30	65930.0
31	81062.0
32	106384.0
33	147491.0
34	210569.0
35	300576.0
36	416910.0
37	536968.0
38	402566.0
39	88485.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.746603130101555	17.422449096543545	13.075065424735472	19.755882348619433
2	13.371990985347887	26.75363842844271	32.15240607350804	27.721964512701362
3	30.490662998862867	22.158391404649645	29.26441857023946	18.086527026248028
4	11.10990762090793	14.847295451541365	44.18441304762884	29.858383879921867
5	11.080729386589432	42.652342909512555	34.129761255211136	12.13716644868687
6	27.507826343660017	37.95554533991997	15.367392033909383	19.169236282510628
7	25.37311734118626	33.076272670565956	22.614338147422973	18.936271840824805
8	23.793021282595134	24.05087086189609	20.295952426146822	31.860155429361953
9	19.874344488637803	23.818262125239155	26.34190973319866	29.96548365292438
10-11	27.039847674827172	21.91469746263007	26.833873846353807	24.211581016188955
12-13	33.58470961541815	21.731375560020524	23.69372602616504	20.990188798396282
14-15	24.115725049466523	25.10011716287402	31.151904548423587	19.632253239235865
16-17	20.300436614523697	27.555768266607476	29.42034341330376	22.72345170556507
18-19	19.01750334343324	35.39673724974735	24.604419298195694	20.981340108623716
20-21	24.153811782428704	25.715416553689813	21.87577618999277	28.254995473888716
22-23	20.926528715106116	27.02897981391886	24.759009427416093	27.285482043558932
24-25	28.070449830572137	26.432050513166615	22.60247958173465	22.895020074526602
26-27	25.244544390646873	21.36827370981436	31.91981458959591	21.467367309942862
28-29	19.955023919931563	34.55170780601174	24.59776698351856	20.895501290538135
30-31	23.071113413636983	25.01464066215814	24.014614593518306	27.89963133068657
32-33	24.32470439294626	26.674176478622734	26.21949383671593	22.78162529171507
34-35	28.292888991126	24.208720600738054	25.60876719491143	21.88962321322452
36-37	24.806960705852674	22.326646518646463	31.598224168848265	21.268168606652594
38-39	22.33714692569278	28.28778674923228	28.500430906438407	20.87463541863653
40-41	22.08026761379232	29.852230405634284	25.292789794837383	22.774712185736014
42-43	20.735910181215143	29.506417516043793	20.925284925080017	28.83238737766105
44-45	24.348216312054134	23.366161525280695	23.651060973182435	28.63456118948274
46-47	28.44589733598874	28.079867087722032	24.981977591618655	18.49225798467058
48-49	25.665846359278476	26.377662862110135	26.055036370306905	21.90145440830448
50-51	16.97356391131524	28.149962237306898	33.047084835776815	21.82938901560105
52-53	23.107515878234523	31.30307013782835	22.23984787302627	23.34956611091086
54-55	20.0376962397046	25.054208544236158	26.297736389284925	28.610358826774316
56-57	26.320277030418143	22.0610647351274	25.804214771662377	25.81444346279208
58-59	31.9027526888118	23.252187399747427	23.272405479558714	21.572654431882057
60-61	24.03935887844495	26.03561933325033	31.48462368209679	18.44039810620793
62-63	20.51569214406724	26.81636608894192	29.259420850314164	23.408520916676675
64-65	19.092495829281976	34.387503546372784	24.212879691426547	22.307120932918696
66-67	23.976668754601217	25.29638295180324	21.921017310758668	28.805930982836887
68-69	20.908381889437706	26.579662648549174	25.039964785078983	27.471990676934134
70-71	27.624020034263335	26.019053264630372	22.8109561138054	23.545970587300896
72-73	24.992705190727357	21.3059297020945	31.806451861695923	21.894913245482222
74-75	20.173357850217215	33.761662613472154	24.740267145398427	21.3247123909122
76-77	22.835567037381598	24.715334493676814	24.27207055201768	28.17702791692391
78-79	24.12340415685897	26.028554670470626	26.48321915456168	23.36482201810872
80-81	27.75844198392591	23.86174899233906	25.740016498680323	22.63979252505471
82-83	24.40072867103845	22.110318003940847	31.31337078300918	22.175582542011522
84-85	22.113007314346493	27.8795740373753	28.255703177678832	21.751715470599382
86-87	21.89109430681929	29.42807075724531	25.46039346428488	23.220441471650517
88-89	20.74783457832876	28.82894164952698	21.427956291084527	28.995267481059734
90-91	24.13140277655105	22.985514948067518	23.985602905420507	28.897479369960934
92-93	28.003516892911783	27.469684427171742	25.0381951611138	19.48860351880267
94-95	24.881563236729185	25.959017335711803	26.22738462905852	22.932034798500492
96-97	17.320339329765318	27.44613549465881	32.717010595700096	22.51651457987577
98-99	23.1364520756319	30.368332024717056	22.88779049995997	23.607425399691074
100	22.395925716600363	19.882109311278498	23.702557921668905	34.01940705045223
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1024.0
1	742.0
2	476.0
3	439.0
4	344.0
5	265.5
6	221.5
7	208.0
8	212.0
9	226.0
10	209.0
11	204.5
12	200.0
13	199.0
14	237.5
15	277.0
16	326.5
17	385.5
18	469.5
19	599.5
20	747.0
21	1011.5
22	1594.0
23	2311.0
24	3150.5
25	4191.0
26	5577.5
27	7103.0
28	8642.0
29	10885.5
30	13763.5
31	16601.5
32	19822.0
33	22927.5
34	25597.5
35	28715.5
36	32286.5
37	36240.0
38	38608.5
39	39410.0
40	39859.5
41	39907.0
42	40958.5
43	44562.0
44	49989.0
45	57491.0
46	74613.0
47	196036.0
48	462944.0
49	478263.5
50	246718.5
51	127171.5
52	84629.0
53	68412.0
54	61020.0
55	53932.5
56	47906.5
57	42205.0
58	35772.5
59	31327.5
60	26550.0
61	21042.0
62	16715.5
63	12974.0
64	9372.0
65	6461.0
66	4828.0
67	3705.0
68	2728.0
69	2112.0
70	1729.5
71	1397.5
72	1110.0
73	913.5
74	727.5
75	553.5
76	412.0
77	266.5
78	156.5
79	98.0
80	65.0
81	47.0
82	32.0
83	25.5
84	21.5
85	18.0
86	14.5
87	9.0
88	7.0
89	8.5
90	10.0
91	8.5
92	6.5
93	5.0
94	3.5
95	5.0
96	5.0
97	5.0
98	6.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059218195001514415
2	0.009508687232109257
3	0.011234201903573101
4	0.00972896570080677
5	0.0121520288564794
6	0.0121520288564794
7	0.0030838985617651646
8	0.005286683248740282
9	0.006902058685855368
10-11	0.0065349279046928496
12-13	0.0055987444127284245
14-15	0.009765678778923021
16-17	0.009380191458702378
18-19	0.007434398318541022
20-21	0.006773562912448486
22-23	0.0024597762337888814
24-25	0.00365295127256707
26-27	0.005048048240984645
28-29	0.005690527108019054
30-31	0.009031417216597983
32-33	0.010169522638201794
34-35	0.01132598459886373
36-37	0.017640634034859066
38-39	0.018209686745660973
40-41	0.018264756362835352
42-43	0.023973640009912534
44-45	0.02786522629023524
46-47	0.03032500252402412
48-49	0.03164667333620919
50-51	0.03302341376556864
52-53	0.030710489844244765
54-55	0.03697006966306573
56-57	0.040072324763889014
58-59	0.037245417748937616
60-61	0.03796132277220453
62-63	0.03311519646085927
64-65	0.035703468468055034
66-67	0.038126531623727665
68-69	0.04095343863867906
70-71	0.03999889860765651
72-73	0.036658008499077584
74-75	0.03948491551402898
76-77	0.039209567428157094
78-79	0.03847530586583205
80-81	0.04343157141152607
82-83	0.03891586280322708
84-85	0.035134415757253123
86-87	0.03339054454673116
88-89	0.031444751406569806
90-91	0.03357410993731242
92-93	0.03550154653841565
94-95	0.039062715115692086
96-97	0.034069736491881816
98-99	0.031095977164465414
100	0.029150184024304058
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2723825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.22206686945409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91352343463771	41.7041021083825
2	13.358478806504412	14.486486622409348
3	4.734955748993276	7.7021726173745835
4	2.0876581655496627	4.5278856261198275
5	1.0692655730451122	2.898889470142861
6	0.5917286687188739	1.9250850866308695
7	0.37942825988681417	1.440136913582041
8	0.24572427074397346	1.0658942271782057
9	0.15480797338059601	0.7554607456110611
>10	0.45960389751807895	3.5307788441545243
>50	0.002949474576247017	0.10701174405188314
>100	3.752104679861968E-4	0.04442950128809232
>500	7.502579886374655E-5	0.03112061220437073
>1k	4.5015479318247936E-4	0.359249838401043
>5k	3.001031954549862E-4	1.0900350379295605
>10k+	6.752321897737191E-4	18.331261004539236
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	188724	6.928638954411535	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	47377	1.739355501913669	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	43758	1.6064908722109534	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	35844	1.3159435719989354	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	33130	1.2163042779914275	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	30405	1.116261140124641	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	29485	1.0824851082576892	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	25540	0.937652015089075	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	16352	0.6003322533569522	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	8440	0.30985837930116655	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	7860	0.2885647939937404	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	5326	0.19553385404715795	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5169	0.1897699007829064	No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC	3007	0.11039622589556965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.6713078116251964E-5	0.0
8	0.0	0.0	0.0	3.6713078116251964E-5	0.0
9	0.0	0.0	0.0	3.6713078116251964E-5	0.0
10-11	0.0	0.0	0.0	7.342615623250393E-5	0.0
12-13	0.0	0.0	0.0	2.3863500775563777E-4	0.0
14-15	0.0	0.0	0.0	4.2220039833689755E-4	0.0
16-17	0.0	0.0	0.0	6.791919451506613E-4	3.6713078116251964E-5
18-19	0.0	0.0	0.0	0.0011013923434875588	3.6713078116251964E-5
20-21	0.0	0.0	0.0	0.002019219296393858	5.5069617174377946E-5
22-23	0.0	1.8356539058125982E-5	0.0	0.0032123943351720467	7.342615623250393E-5
24-25	0.0	3.6713078116251964E-5	0.0	0.004589134764531495	7.342615623250393E-5
26-27	0.0	3.6713078116251964E-5	0.0	0.006700136756215983	7.342615623250393E-5
28-29	0.0	3.6713078116251964E-5	0.0	0.012078602700246896	7.342615623250393E-5
30-31	0.0	3.6713078116251964E-5	0.0	0.022982386900773727	7.342615623250393E-5
32-33	0.0	3.6713078116251964E-5	0.0	0.05367452020596036	7.342615623250393E-5
34-35	0.0	3.6713078116251964E-5	0.0	0.09207639991555992	1.1013923434875588E-4
36-37	0.0	3.6713078116251964E-5	0.0	0.1389039310528393	1.1013923434875588E-4
38-39	0.0	3.6713078116251964E-5	0.0	0.2075023175130561	1.1013923434875588E-4
40-41	0.0	3.6713078116251964E-5	0.0	0.2757152166530522	1.1013923434875588E-4
42-43	0.0	3.6713078116251964E-5	0.0	0.34864574633098677	1.1013923434875588E-4
44-45	0.0	3.6713078116251964E-5	0.0	0.43745468229420026	1.1013923434875588E-4
46-47	0.0	3.6713078116251964E-5	0.0	0.5478509081897699	1.2849577340688187E-4
48-49	0.0	3.6713078116251964E-5	0.0	0.6382935761291566	1.4685231246500786E-4
50-51	0.0	3.6713078116251964E-5	0.0	0.7364827035510725	1.4685231246500786E-4
52-53	0.0	3.6713078116251964E-5	0.0	0.8326526116765944	1.4685231246500786E-4
54-55	0.0	3.6713078116251964E-5	0.0	0.9476379723366957	1.4685231246500786E-4
56-57	0.0	3.6713078116251964E-5	0.0	1.1185557075069066	1.4685231246500786E-4
58-59	0.0	3.6713078116251964E-5	0.0	1.2506310060301231	1.4685231246500786E-4
60-61	0.0	3.6713078116251964E-5	0.0	1.3827246610923978	1.4685231246500786E-4
62-63	0.0	3.6713078116251964E-5	0.0	1.514799959615614	1.4685231246500786E-4
64-65	0.0	3.6713078116251964E-5	0.0	1.6413315833432764	1.4685231246500786E-4
66-67	0.0	3.6713078116251964E-5	0.0	1.8043743632575513	1.4685231246500786E-4
68-69	0.0	3.6713078116251964E-5	0.0	1.9923453232127617	1.4685231246500786E-4
70-71	0.0	3.6713078116251964E-5	0.0	2.173818068341395	1.4685231246500786E-4
72-73	0.0	3.6713078116251964E-5	0.0	2.3206520242673445	1.6520885152313382E-4
74-75	0.0	3.6713078116251964E-5	0.0	2.5025653263333734	1.835653905812598E-4
76-77	0.0	3.6713078116251964E-5	0.0	2.6697750406138425	2.5699154681376374E-4
78-79	0.0	3.6713078116251964E-5	0.0	2.9509237928281	2.5699154681376374E-4
80-81	0.0	3.6713078116251964E-5	0.0	3.220269290427983	2.5699154681376374E-4
82-83	0.0	3.6713078116251964E-5	0.0	3.422852055473461	2.5699154681376374E-4
84-85	0.0	3.6713078116251964E-5	0.0	3.6412214441089277	2.5699154681376374E-4
86-87	0.0	3.6713078116251964E-5	0.0	3.8543775756518865	3.120611639881417E-4
88	0.0	3.6713078116251964E-5	0.0	4.049966499316219	3.671307811625196E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	560	0.0	33.58332	1
GAGTACG	2415	0.0	28.813515	1
GTACGCA	2380	0.0	28.63145	3
AGTACGC	2495	0.0	27.12341	2
TACGCAG	2550	0.0	26.354097	4
TAGTACT	955	0.0	24.604752	4
CTAGTAC	980	0.0	24.45662	3
CGCAGTG	2795	0.0	23.706404	6
GCATCGT	700	0.0	23.496672	8
TAGGCAT	1000	0.0	21.617336	5
GTACCCG	240	6.679329E-9	21.5493	1
CATGGGG	3670	0.0	21.00052	4
CCTAGTA	1100	0.0	20.934168	2
CAATGCG	405	0.0	20.305395	18-19
TGGGGGG	2400	0.0	19.971806	6
TACCTGG	1935	0.0	19.672356	2
CTAGGCA	1130	0.0	19.546623	4
TACTAGG	950	0.0	19.292713	2
ACGCAGT	3510	0.0	18.878014	5
GTATAAC	325	5.6024874E-10	18.80666	1
>>END_MODULE