FastQCFastQC Report
Mon 16 Jan 2017
ERR1146547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723825
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1844596.772057676245721No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC476181.7482033537396862No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT434371.5947059741356364No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC362941.3324644571512487No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA341671.2543757399979807No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA318941.17092691343974No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG310491.139904362431507No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC267460.981927987297275No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG172830.6345121290831827No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT148150.5439042522922728No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA91350.33537396859196167No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG85330.313272695565978No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52660.19333106936018282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45620.16748506236634145No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA45210.16597982616357512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.16194138757078738No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT36810.13514084054592346No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32780.12034547006507393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6250.028.6139431
GAGTACG23400.024.7379321
GTACGCA23300.024.2141153
CTAGTAC7950.023.6557183
ATACGGG4650.023.2550963
GAGTACT51400.023.08692412-13
CGTTAAC2052.948218E-822.9572721
TACGCAG24650.022.8879874
AGTACGC24700.022.8626462
GTGATCG4650.022.2350238
CGCAGTG25600.022.0297286
TACGGGC4650.021.2329144
AGTAGGC15700.020.681962
GCTTATG19350.020.6734091
TCGACGG1605.4184715E-520.56145594
TGATCGC4800.020.5610799
GCTATTG9850.020.5163488
CGTAGAC1405.2012905E-420.1496033
CGGGCGT4900.020.1414666
GTACTTT58900.020.10726714-15
Produced by FastQC (version 0.11.5)