FastQCFastQC Report
Mon 16 Jan 2017
ERR1146546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006444
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT270800.900731894557158No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68730.22860894797973952No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT65680.21846407250559133No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG58620.19498118042444829No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA48110.1600229373971376No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA42360.14089735248685822No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC39510.13141771474871974No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31650.10527387172353785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13550.024.291041
CATGGGG64250.021.6497574
TGATCGC9100.021.172389
GTGATCG9200.020.431468
GCATCGT13750.019.8222318
ATGGGGG43100.018.9717085
GTATAAG16050.018.7496131
CTGTGCG18050.018.4845289
GTACATG172950.018.215521
TCTAACG6450.018.2146992
GAGTACG7000.018.1364861
CCGTATC2354.9039954E-918.00186394
ACATGGG164000.017.8806133
GTACCCG2905.8635123E-817.8353671
CTAGTAC17950.017.802713
TACATGG171850.017.7748322
CTCGTCG9550.017.7143969
CTAACGC6400.017.622723
CAATGCG8600.017.21117218-19
TGGGGGG40200.017.0665916
Produced by FastQC (version 0.11.5)