##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146546_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006444
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15075983454207	31.0	31.0	34.0	28.0	34.0
2	31.36148819003447	31.0	31.0	34.0	28.0	34.0
3	31.397926254405537	33.0	31.0	34.0	28.0	34.0
4	34.95307446272074	37.0	35.0	37.0	32.0	37.0
5	35.034075805170495	37.0	35.0	37.0	32.0	37.0
6	35.04377696707472	37.0	35.0	37.0	32.0	37.0
7	35.02327101386222	37.0	35.0	37.0	32.0	37.0
8	34.98661209056281	37.0	35.0	37.0	32.0	37.0
9	36.56146164704881	39.0	37.0	39.0	32.0	39.0
10-11	36.562358553826385	39.0	37.0	39.0	32.0	39.0
12-13	36.53919414431135	39.0	37.0	39.0	32.0	39.0
14-15	37.855055341127255	40.0	38.0	41.0	32.0	41.0
16-17	37.849012487842785	40.0	38.0	41.0	32.0	41.0
18-19	37.85588073484821	40.0	38.0	41.0	32.0	41.0
20-21	37.947118921889114	40.0	38.0	41.0	32.0	41.0
22-23	37.93268509242148	40.0	38.0	41.0	32.0	41.0
24-25	37.95748382474445	40.0	38.0	41.0	32.0	41.0
26-27	37.95952444149965	40.0	38.0	41.0	32.0	41.0
28-29	37.91410649923964	40.0	38.0	41.0	32.0	41.0
30-31	37.90936668037057	40.0	38.0	41.0	32.0	41.0
32-33	37.90351475031632	40.0	38.0	41.0	32.0	41.0
34-35	37.82105670353414	40.0	38.0	41.0	31.5	41.0
36-37	37.722968896144415	40.0	38.0	41.0	31.0	41.0
38-39	37.66270467702043	40.0	38.0	41.0	31.0	41.0
40-41	37.6187956602551	40.0	38.0	41.0	31.0	41.0
42-43	37.468871863237766	40.0	37.0	41.0	30.5	41.0
44-45	37.292550268689524	40.0	37.0	41.0	30.0	41.0
46-47	37.154077541440984	40.0	36.5	41.0	30.0	41.0
48-49	37.019531213619814	40.0	36.0	41.0	30.0	41.0
50-51	36.86824800328894	39.5	36.0	41.0	30.0	41.0
52-53	36.770505287974764	39.0	35.5	41.0	30.0	41.0
54-55	36.581746575023516	39.0	35.0	41.0	29.0	41.0
56-57	36.37527540842271	39.0	35.0	41.0	29.0	41.0
58-59	36.14726617226198	39.0	35.0	40.0	28.5	41.0
60-61	35.92246038841901	38.0	35.0	40.0	28.0	41.0
62-63	35.70827412717483	38.0	34.5	40.0	28.0	41.0
64-65	35.472284200204626	37.0	34.0	40.0	28.0	41.0
66-67	35.17852935228463	37.0	34.0	40.0	28.0	41.0
68-69	34.84378089197736	36.0	34.0	39.0	28.0	41.0
70-71	34.521706208397696	36.0	34.0	39.0	27.0	41.0
72-73	34.22066966821933	35.0	33.5	38.5	26.5	40.0
74-75	33.92563407134808	35.0	33.0	37.5	26.0	40.0
76-77	33.618875655092864	35.0	33.0	37.0	26.0	39.0
78-79	33.31955825553378	35.0	33.0	36.5	26.0	39.0
80-81	33.01235479523317	35.0	33.0	36.0	26.0	38.5
82-83	32.75823115281708	35.0	33.0	36.0	26.0	37.0
84-85	32.563217542053	35.0	32.0	35.0	25.5	37.0
86-87	32.42557686090278	35.0	32.0	35.0	25.0	36.5
88-89	32.27249019106958	35.0	32.0	35.0	25.0	36.0
90-91	32.090849854512506	35.0	32.0	35.0	25.0	36.0
92-93	31.940486667970532	35.0	32.0	35.0	25.0	36.0
94-95	31.85063217542053	35.0	32.0	35.0	25.0	35.0
96-97	31.797887304736093	35.0	32.0	35.0	25.0	35.0
98-99	31.75538260483149	35.0	32.0	35.0	24.0	35.0
100	30.719105361683106	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25917755656475094
1101	2	0.3977795452134849
1101	3	0.3326310953658691
1101	4	0.35859236736637
1101	5	0.35932158722353336
1101	6	0.30608866713154725
1101	7	0.2182787645529416
1101	8	0.3214820190157326
1101	9	0.39699201522952876
1101	10-11	0.46222592531271545
1101	12-13	0.45863155482941664
1101	14-15	0.5427990529833266
1101	16-17	0.5193738397817711
1101	18-19	0.4742362664282638
1101	20-21	0.5078416570762556
1101	22-23	0.47641707490865315
1101	24-25	0.5073616899062756
1101	26-27	0.5452562958641991
1101	28-29	0.6058895624160101
1101	30-31	0.6173759448051044
1101	32-33	0.6338683149325632
1101	34-35	0.6299198484898696
1101	36-37	0.5563802487224052
1101	38-39	0.6110824262389798
1101	40-41	0.600929972674912
1101	42-43	0.6151844041025285
1101	44-45	0.5416220106479273
1101	46-47	0.4794228300370946
1101	48-49	0.5073811006823234
1101	50-51	0.4651964991262787
1101	52-53	0.48800286659667336
1101	54-55	0.5015527839456695
1101	56-57	0.4484629307935677
1101	58-59	0.494557682422311
1101	60-61	0.4826579381494369
1101	62-63	0.49655879688626925
1101	64-65	0.4699869100595322
1101	66-67	0.46044002130330597
1101	68-69	0.4344590898306038
1101	70-71	0.4323394414474322
1101	72-73	0.4588087463772297
1101	74-75	0.4262962353145596
1101	76-77	0.4104526913035116
1101	78-79	0.4066537074790233
1101	80-81	0.4329528867018695
1101	82-83	0.45845240606047355
1101	84-85	0.4548210294954558
1101	86-87	0.4877629516899802
1101	88-89	0.3928008767111848
1101	90-91	0.42673701778392115
1101	92-93	0.33880555907261467
1101	94-95	0.375200294286568
1101	96-97	0.3602545159381414
1101	98-99	0.3595504361399122
1101	100	0.28138981951621744
1102	1	0.18594513097084686
1102	2	0.24239977657326506
1102	3	0.24999733009417113
1102	4	0.13645114829251526
1102	5	0.15761635331097779
1102	6	0.17548161071537294
1102	7	0.13613545485841172
1102	8	0.21580652166144176
1102	9	0.2679710744031709
1102	10-11	0.29034211811097066
1102	12-13	0.2616622153613548
1102	14-15	0.32096530976061644
1102	16-17	0.3986823753960991
1102	18-19	0.28662174864739853
1102	20-21	0.25957440180911817
1102	22-23	0.33370525755763936
1102	24-25	0.3001940540587924
1102	26-27	0.33883022535381
1102	28-29	0.40248951408301537
1102	30-31	0.3758929552982053
1102	32-33	0.4161742359265972
1102	34-35	0.420670599785673
1102	36-37	0.45427524242533224
1102	38-39	0.43595368669166135
1102	40-41	0.37574575773489727
1102	42-43	0.432390413931131
1102	44-45	0.35868483648813054
1102	46-47	0.30607024417123796
1102	48-49	0.371540501433806
1102	50-51	0.2975882128739329
1102	52-53	0.3499507805639297
1102	54-55	0.3291417168317068
1102	56-57	0.2852769122730834
1102	58-59	0.2770212402486436
1102	60-61	0.26530017345988455
1102	62-63	0.22777364580531412
1102	64-65	0.2320372711621559
1102	66-67	0.3477356017916975
1102	68-69	0.25897470948955714
1102	70-71	0.3509981301442693
1102	72-73	0.2768509036423197
1102	74-75	0.2536625944045383
1102	76-77	0.23926561201151486
1102	78-79	0.25371220082719503
1102	80-81	0.27843060865133396
1102	82-83	0.2564577455460153
1102	84-85	0.3135913344807122
1102	86-87	0.301112774709857
1102	88-89	0.33751007708911374
1102	90-91	0.3543633936190389
1102	92-93	0.30317953413910814
1102	94-95	0.24769777596254627
1102	96-97	0.27688587397104314
1102	98-99	0.3246664134899504
1102	100	0.18221527857047093
1103	1	0.1643657862874477
1103	2	0.24899039849167792
1103	3	0.2968981424481498
1103	4	0.1663291738110857
1103	5	0.29980142019405775
1103	6	0.3051836859347077
1103	7	0.31784806859894843
1103	8	0.3125936368279625
1103	9	0.3803370741465315
1103	10-11	0.4524845962356707
1103	12-13	0.4245639271196211
1103	14-15	0.48741752977479536
1103	16-17	0.503706900925657
1103	18-19	0.3853799233422279
1103	20-21	0.39944188418229487
1103	22-23	0.34807838548460523
1103	24-25	0.3059615707665557
1103	26-27	0.3572830418880528
1103	28-29	0.40804229512662005
1103	30-31	0.4176825964266442
1103	32-33	0.4299546009367674
1103	34-35	0.4588377687546057
1103	36-37	0.47871349045976785
1103	38-39	0.49218003831249746
1103	40-41	0.48265001510787187
1103	42-43	0.42381803905294646
1103	44-45	0.32996632575365936
1103	46-47	0.37298395800576145
1103	48-49	0.37287631333114746
1103	50-51	0.32162906987442597
1103	52-53	0.2923285026202649
1103	54-55	0.3371221958280799
1103	56-57	0.37992302905460207
1103	58-59	0.38248419646800613
1103	60-61	0.3446754920952415
1103	62-63	0.296950320945804
1103	64-65	0.3415631533124497
1103	66-67	0.27912972468176633
1103	68-69	0.23994386963322967
1103	70-71	0.2376226126688934
1103	72-73	0.23398035890158297
1103	74-75	0.2504649693867691
1103	76-77	0.2008498930140732
1103	78-79	0.17400740889824817
1103	80-81	0.26814815177476703
1103	82-83	0.2722536778802933
1103	84-85	0.31258268378366694
1103	86-87	0.34192468938899623
1103	88-89	0.33488614021850793
1103	90-91	0.3268113339485339
1103	92-93	0.3021321558794874
1103	94-95	0.2168498273095132
1103	96-97	0.28486346285079733
1103	98-99	0.21923524855331067
1103	100	0.1874914754544612
1104	1	0.11356024847580315
1104	2	0.18138699608371311
1104	3	0.2418795407680605
1104	4	0.17848705073362936
1104	5	0.1693929607541378
1104	6	0.1694841532682574
1104	7	0.18889942464377185
1104	8	0.16765764151592322
1104	9	0.262720992422544
1104	10-11	0.293029624927307
1104	12-13	0.26799746806560876
1104	14-15	0.37727368462865485
1104	16-17	0.29855574600225054
1104	18-19	0.3292644092450061
1104	20-21	0.32167318343947215
1104	22-23	0.3907780775787444
1104	24-25	0.3365586684693298
1104	26-27	0.36034319748520005
1104	28-29	0.3450491883232729
1104	30-31	0.4100287299842478
1104	32-33	0.41057067303089667
1104	34-35	0.32594970946034607
1104	36-37	0.37149676617443106
1104	38-39	0.39361708096100756
1104	40-41	0.39178978682311794
1104	42-43	0.32921117096527297
1104	44-45	0.2734481408325209
1104	46-47	0.2689352794187556
1104	48-49	0.31394959335784733
1104	50-51	0.3235167899836071
1104	52-53	0.3039751944600937
1104	54-55	0.2911528986759109
1104	56-57	0.23014071739594044
1104	58-59	0.27519533739602053
1104	60-61	0.35862291805142377
1104	62-63	0.3463675539017501
1104	64-65	0.37883722163238787
1104	66-67	0.28048601916860605
1104	68-69	0.27556561442838046
1104	70-71	0.28734167041202596
1104	72-73	0.20697065164576145
1104	74-75	0.24202725015140203
1104	76-77	0.21931613090229973
1104	78-79	0.23768450327693813
1104	80-81	0.21568253305089513
1104	82-83	0.23277793817327108
1104	84-85	0.2589712900183301
1104	86-87	0.21505894854423246
1104	88-89	0.14750038335250082
1104	90-91	0.11103630317757052
1104	92-93	0.2042811840304246
1104	94-95	0.1772258055874083
1104	96-97	0.20841530128029362
1104	98-99	0.13773212366365328
1104	100	0.15403627088948113
1105	1	0.22603578149224646
1105	2	0.23387819133423804
1105	3	0.22919514992550205
1105	4	0.24668107962605035
1105	5	0.16995008318733085
1105	6	0.2375361397728284
1105	7	0.2429436652679513
1105	8	0.26875043702843726
1105	9	0.3105144146767671
1105	10-11	0.35133146416033156
1105	12-13	0.2491213076625769
1105	14-15	0.4137578299111553
1105	16-17	0.39719608127387573
1105	18-19	0.29986483133464503
1105	20-21	0.326813738391742
1105	22-23	0.3407198278628414
1105	24-25	0.3056024282354457
1105	26-27	0.41447312337137276
1105	28-29	0.4400777327869392
1105	30-31	0.35964035648734693
1105	32-33	0.3806216552010184
1105	34-35	0.3543152602839612
1105	36-37	0.3597803440163858
1105	38-39	0.2377022323724205
1105	40-41	0.3366653679534082
1105	42-43	0.3730397993197485
1105	44-45	0.25558640869485316
1105	46-47	0.2580438863294887
1105	48-49	0.2210292820534221
1105	50-51	0.24410864635088103
1105	52-53	0.16205639975116526
1105	54-55	0.31725269677159673
1105	56-57	0.23107484376656373
1105	58-59	0.3022063264747814
1105	60-61	0.19596355145504418
1105	62-63	0.26351001770631655
1105	64-65	0.19694560732037303
1105	66-67	0.1510190930909303
1105	68-69	0.1524184811135001
1105	70-71	0.22382732976657138
1105	72-73	0.25494122032841915
1105	74-75	0.20633429162380423
1105	76-77	0.20517245499895154
1105	78-79	0.20400845693399816
1105	80-81	0.20952267115669088
1105	82-83	0.2099551246965774
1105	84-85	0.2662268914540036
1105	86-87	0.1994458096513867
1105	88-89	0.15322890294613245
1105	90-91	0.247569936185009
1105	92-93	0.2376988007071965
1105	94-95	0.2072665649674299
1105	96-97	0.19514115134061072
1105	98-99	0.21441448901428117
1105	100	0.21788603456806754
1106	1	0.1481016084219604
1106	2	0.12619307203771157
1106	3	0.1470214326853423
1106	4	0.10471487635162902
1106	5	0.1772835880050323
1106	6	0.1850436305908616
1106	7	0.12164190043632317
1106	8	0.1612252863604624
1106	9	0.20766290145239452
1106	10-11	0.18574505065752334
1106	12-13	0.22570392821371854
1106	14-15	0.2800984080566593
1106	16-17	0.23282367516491576
1106	18-19	0.23469378908967542
1106	20-21	0.19436164516039156
1106	22-23	0.2519342349100384
1106	24-25	0.24833832630613983
1106	26-27	0.27581390402578876
1106	28-29	0.2483026791489067
1106	30-31	0.32717635412269175
1106	32-33	0.25556795010601974
1106	34-35	0.2944469474031308
1106	36-37	0.24589351672483417
1106	38-39	0.28849567837938395
1106	40-41	0.25140254491481784
1106	42-43	0.23212736658690858
1106	44-45	0.1704382781203293
1106	46-47	0.21412777705407393
1106	48-49	0.19083120688050315
1106	50-51	0.16507153733054736
1106	52-53	0.20026653211688483
1106	54-55	0.25463291811517763
1106	56-57	0.12462540434073333
1106	58-59	0.19136399937649173
1106	60-61	0.25061046040463
1106	62-63	0.18259866859731488
1106	64-65	0.1683713049233262
1106	66-67	0.14703854574823083
1106	68-69	0.15501201676083554
1106	70-71	0.12926143511035804
1106	72-73	0.16196025965297167
1106	74-75	0.12922490650255014
1106	76-77	0.07795836874732487
1106	78-79	0.08608027598169343
1106	80-81	0.16042281656076796
1106	82-83	0.1391274318846243
1106	84-85	0.1566679880491435
1106	86-87	0.2006877927795827
1106	88-89	0.13152665327330482
1106	90-91	0.12195210295525527
1106	92-93	0.04733739338024279
1106	94-95	0.13319494762397355
1106	96-97	0.12125096052357875
1106	98-99	0.13527070741920255
1106	100	0.12410354094039278
1107	1	0.15831910127423754
1107	2	0.06461038955872667
1107	3	0.15012511765041125
1107	4	0.14640701428341885
1107	5	0.1437418828534831
1107	6	0.10318070525794099
1107	7	0.08212270727467796
1107	8	0.18343250895511432
1107	9	0.2327254086853543
1107	10-11	0.25645515139309083
1107	12-13	0.11549842920074127
1107	14-15	0.21235485460576342
1107	16-17	0.20150676632230358
1107	18-19	0.18331071524826825
1107	20-21	0.16011263834312928
1107	22-23	0.19680671386362292
1107	24-25	0.24151188350282382
1107	26-27	0.2237275366121807
1107	28-29	0.20355492800645436
1107	30-31	0.18095011875597322
1107	32-33	0.16790082338801682
1107	34-35	0.20945225508290832
1107	36-37	0.27780636311607054
1107	38-39	0.2330722179730813
1107	40-41	0.1495111002208489
1107	42-43	0.1257603020349336
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2216	68-69	-0.8587990660036411
2216	70-71	-0.912288884934803
2216	72-73	-0.8331617156606512
2216	74-75	-0.7733655739876681
2216	76-77	-0.8240790160252729
2216	78-79	-0.8664224952769217
2216	80-81	-0.9042399264156558
2216	82-83	-0.9138499318613
2216	84-85	-0.8077640584197212
2216	86-87	-0.7935365553703697
2216	88-89	-0.8387178480325588
2216	90-91	-0.8466702925135436
2216	92-93	-0.8192762021601894
2216	94-95	-0.7583829904250976
2216	96-97	-0.6831206920545441
2216	98-99	-0.7504952802313021
2216	100	-0.7258945463972353
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	94.0
1	53.0
2	29.0
3	28.0
4	39.0
5	44.0
6	122.0
7	611.0
8	1884.0
9	2568.0
10	2888.0
11	3798.0
12	5519.0
13	6942.0
14	8153.0
15	8967.0
16	9198.0
17	9826.0
18	10870.0
19	11674.0
20	12661.0
21	14285.0
22	16225.0
23	18886.0
24	22920.0
25	27738.0
26	30949.0
27	30769.0
28	33584.0
29	40603.0
30	47420.0
31	55165.0
32	69819.0
33	93965.0
34	140221.0
35	216338.0
36	388488.0
37	717242.0
38	750784.0
39	194873.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39707416757222	19.702224744268403	12.98519111433578	23.9155099738236
2	14.466168885127695	21.543621749899707	39.325174027273526	24.665035337699067
3	19.40170507997802	26.308050761191666	30.120229827855315	24.170014330974997
4	11.909055496731524	17.291637351674456	40.286999179346545	30.512307972247477
5	12.404081479221855	39.391337584957355	35.606225676274015	12.598355259546775
6	29.32547554092458	38.36375533373274	18.505852926529794	13.804916198812881
7	25.84809681111541	32.95462369804703	23.364186925736167	17.833092565101392
8	25.227457794903458	33.750039916547806	21.20117540927767	19.82132687927107
9	25.666843966146114	16.431237573651632	21.44778164421483	36.454136815987425
10-11	24.46106882112734	25.90754926106736	28.715253799778328	20.916128118026975
12-13	26.028360549102064	23.991489739637725	27.839768003596504	22.14038170766371
14-15	22.99379168772086	25.42808347340818	26.517806442899527	25.060318395971432
16-17	21.903361754063933	28.124314943520574	27.361026885317013	22.61129641709848
18-19	21.590267046481777	29.29233405833512	27.56066255749161	21.556736337691493
20-21	23.252200518723992	27.635957146226342	26.23660985894925	22.87523247610041
22-23	22.740154525944366	27.388190220985138	26.255972346588365	23.61568290648213
24-25	23.343988663881422	27.60481252827377	26.20531081188962	22.845887995955188
26-27	23.143858521659716	26.801198223653888	27.340352298776878	22.714590955909514
28-29	22.284680915964007	28.557521845712614	26.692925263953953	22.464871974369427
30-31	22.874872199682354	27.326705929452395	26.53273476130385	23.2656871095614
32-33	22.843377093069055	27.8229653452699	26.6338712596204	22.699786302040643
34-35	23.384818717236016	27.60360850660673	26.77997321081208	22.231599565345174
36-37	23.217691830807205	26.76154057150233	27.41233071330585	22.608436884384613
38-39	23.082691270940046	27.533859957845863	27.066075801260432	22.31737296995366
40-41	22.852482473123125	27.581092214651697	27.011958380955214	22.554466931269957
42-43	22.704900143327293	27.424287389194156	26.136620722971095	23.734191744507452
44-45	22.94007822415107	27.120084602259087	26.618885754178002	23.32095141941184
46-47	23.413054110976912	27.602337721968794	26.671740714492483	22.312867452561814
48-49	23.282750075362486	27.23780009409498	26.881516858904785	22.597932971637753
50-51	21.81987691407792	27.45724741441539	27.642959325880568	23.07991634562612
52-53	22.595296206750902	28.060590704973414	26.028007554789777	23.31610553348591
54-55	22.325766571185813	27.099243689803515	26.683047647758663	23.891942091252012
56-57	23.383777564002525	26.464520388269396	26.6398645296388	23.51183751808928
58-59	23.760369730825804	26.732680679920602	26.736107933876625	22.77084165537697
60-61	23.083639417635414	27.28177341393096	27.44633271991693	22.188254448516695
62-63	22.46820428051038	27.18801864355122	27.105385042303347	23.23839203363505
64-65	21.73586959849123	28.300798172866916	26.61469625642684	23.34863597221501
66-67	23.185473539764057	26.762701385750916	26.094249429840993	23.957575644644034
68-69	22.634590981608536	26.826389567105696	26.58635766267789	23.952661788607877
70-71	23.186015271422633	26.75703384210346	26.40504641755132	23.651904468922584
72-73	23.14466639315434	26.122939465599504	27.726630085224713	23.005764056021448
74-75	22.44724487422523	28.105853317507712	26.61784215838068	22.829059649886382
76-77	22.55953920907207	26.731713566263238	26.58482148427719	24.123925740387502
78-79	22.766091288436073	27.026350294321844	26.816637349002104	23.39092106823998
80-81	23.102100251433868	26.744779985385158	26.973619409789286	23.179500353391695
82-83	22.91601607375573	26.11878826921295	27.946177242222543	23.019018414808777
84-85	22.79943727563146	27.202509234276278	26.891583390979974	23.106470099112293
86-87	22.288798237850283	27.529230523520837	27.032727538913033	23.149243699715846
88-89	22.496513479355457	26.992598744270303	26.444055047681697	24.066832728692546
90-91	22.887895387683688	26.1698986880538	26.689875842664947	24.252330081597567
92-93	23.566726282077944	26.92527554797654	26.775776733352743	22.73222143659277
94-95	22.690646122291298	26.61668663716069	27.37773382531402	23.314933415233988
96-97	21.97112910714978	26.672360834087943	28.042238433334237	23.31427162542804
98-99	22.51617828613561	27.309715589231654	26.669255258993154	23.504850865639582
100	22.580508855225464	25.841288008597367	26.33144461782624	25.246758518350926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1335.0
1	965.5
2	583.0
3	560.0
4	488.0
5	376.5
6	306.0
7	296.5
8	301.0
9	310.5
10	306.5
11	278.0
12	274.5
13	287.0
14	306.5
15	362.5
16	428.0
17	541.5
18	744.0
19	976.0
20	1277.5
21	1756.0
22	2495.5
23	3666.5
24	5197.0
25	7028.5
26	9809.0
27	13169.0
28	16970.5
29	22457.0
30	28747.5
31	34555.5
32	42102.0
33	49233.5
34	55083.0
35	61108.5
36	67745.0
37	76079.5
38	80360.0
39	82197.5
40	83117.5
41	83572.5
42	86467.5
43	93149.0
44	105090.5
45	116458.5
46	121656.5
47	140679.0
48	184268.0
49	185845.0
50	153527.5
51	134443.0
52	115962.5
53	105825.0
54	98600.5
55	87655.0
56	78928.0
57	68955.0
58	59151.5
59	53957.5
60	45735.5
61	35111.5
62	26289.0
63	19773.0
64	14526.0
65	9904.5
66	6779.0
67	4889.5
68	3632.5
69	2604.5
70	2117.5
71	1801.0
72	1399.0
73	1120.5
74	851.0
75	585.5
76	424.0
77	316.0
78	224.0
79	139.5
80	92.0
81	69.5
82	53.5
83	54.5
84	49.0
85	29.5
86	20.5
87	25.5
88	20.5
89	11.0
90	13.0
91	16.0
92	10.0
93	5.0
94	8.0
95	8.0
96	6.5
97	5.0
98	4.5
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06083599095808869
2	0.010178137360948683
3	0.012240374342578807
4	0.010078351700547225
5	0.01253973132378318
6	0.011342303398965688
7	0.0031598792460461596
8	0.005721044529683573
9	0.00768349585091224
10-11	0.007184567548904951
12-13	0.006336389435492562
14-15	0.010410970568552082
16-17	0.010144875474148198
18-19	0.0081325313227188
20-21	0.007350876982907382
22-23	0.002927046038442758
24-25	0.00412447396326025
26-27	0.005338532831477985
28-29	0.006336389435492562
30-31	0.009629316228740666
32-33	0.011042946417761317
34-35	0.012656147927584882
36-37	0.018610025664871854
38-39	0.0189759064196772
40-41	0.01864328755167234
42-43	0.024480748685157615
44-45	0.02828923472381325
46-47	0.031731840007663534
48-49	0.03226403019647131
50-51	0.03267980378147739
52-53	0.03104997132825358
54-55	0.03756930114114881
56-57	0.04028014491538841
58-59	0.03733646793354541
60-61	0.038068229443156104
62-63	0.03352798189488977
64-65	0.03592283774452476
66-67	0.03798507472615489
68-69	0.04052960906639206
70-71	0.040679287556994244
72-73	0.03642176604653205
74-75	0.0396149071793787
76-77	0.03913260982077165
78-79	0.038101491329956584
80-81	0.042575215104621936
82-83	0.03899956227356971
84-85	0.03542390944251747
86-87	0.03406017208369755
88-89	0.03128280453585698
90-91	0.03404354114029731
92-93	0.03555695698971942
94-95	0.03914924076417189
96-97	0.03424311246110022
98-99	0.031582161517061356
100	0.02907088906362467
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3006444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.26603438131577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58350671592571	42.87419132207907
2	13.969995820255212	16.279525135396554
3	5.412205878035276	9.46043321305088
4	2.566911504189381	5.982550158275738
5	1.460753820159377	4.255616615402231
6	0.8572451930292891	2.9968966734157343
7	0.5496686817952137	2.24189100182687
8	0.37161053219227663	1.7321817636139392
9	0.2621974260712446	1.3749483817943675
>10	0.9441159343969601	8.434401174468324
>50	0.01581764108276466	0.6261136473373404
>100	0.005181131604638834	0.46378120037650195
>500	0.0	0.0
>1k	4.3873403480149413E-4	0.9060653342659665
>5k	2.6324042088089647E-4	0.9868964646956296
>10k+	8.774680696029883E-5	1.384507914000811
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	27080	0.900731894557158	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	6873	0.22860894797973952	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	6568	0.21846407250559133	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	5862	0.19498118042444829	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	4811	0.1600229373971376	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4236	0.14089735248685822	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	3951	0.13141771474871974	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	3165	0.10527387172353785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	3.326188680048589E-5	0.0
18-19	0.0	0.0	0.0	8.315471700121472E-5	0.0
20-21	0.0	0.0	0.0	1.4967849060218652E-4	0.0
22-23	0.0	0.0	0.0	5.488211322080172E-4	0.0
24-25	0.0	0.0	0.0	0.0012306898116179778	0.0
26-27	0.0	0.0	0.0	0.00214539169863134	0.0
28-29	0.0	0.0	0.0	0.006020401510887946	0.0
30-31	0.0	0.0	0.0	0.016514526796441244	0.0
32-33	0.0	0.0	0.0	0.04064602567019376	0.0
34-35	0.0	0.0	0.0	0.077217470207328	0.0
36-37	0.0	0.0	0.0	0.1279085856912685	0.0
38-39	0.0	0.0	0.0	0.2055917223138033	0.0
40-41	0.0	0.0	0.0	0.28902916535282214	0.0
42-43	0.0	0.0	0.0	0.3680594083907766	0.0
44-45	0.0	0.0	0.0	0.45989547784691814	0.0
46-47	0.0	0.0	0.0	0.5727197978741663	0.0
48-49	0.0	0.0	0.0	0.6824840243157697	0.0
50-51	0.0	0.0	0.0	0.801278853023705	0.0
52-53	0.0	0.0	0.0	0.912855852295935	0.0
54-55	0.0	0.0	0.0	1.0368395353447462	0.0
56-57	0.0	0.0	0.0	1.1799820651906372	0.0
58-59	0.0	0.0	0.0	1.3308746146610413	0.0
60-61	0.0	0.0	0.0	1.485209769415296	0.0
62-63	0.0	0.0	0.0	1.6412745422831758	0.0
64-65	0.0	0.0	0.0	1.8054884774171747	0.0
66-67	0.0	0.0	0.0	1.98383871444138	0.0
68-69	0.0	0.0	0.0	2.1822292382628783	0.0
70-71	0.0	0.0	0.0	2.398165407371632	0.0
72-73	0.0	0.0	0.0	2.5975704187405455	0.0
74-75	0.0	0.0	0.0	2.8033450814317513	0.0
76-77	0.0	0.0	0.0	3.0166701924266675	0.0
78-79	0.0	0.0	0.0	3.2745662317342346	0.0
80-81	0.0	0.0	0.0	3.5381167917978846	0.0
82-83	0.0	0.0	0.0	3.7932188326142113	0.0
84-85	0.0	0.0	0.0	4.052777966261803	0.0
86-87	0.0	0.0	0.0	4.313301694626609	0.0
88	0.0	0.0	0.0	4.526377341470521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1355	0.0	24.29104	1
CATGGGG	6425	0.0	21.649757	4
TGATCGC	910	0.0	21.17238	9
GTGATCG	920	0.0	20.43146	8
GCATCGT	1375	0.0	19.822231	8
ATGGGGG	4310	0.0	18.971708	5
GTATAAG	1605	0.0	18.749613	1
CTGTGCG	1805	0.0	18.484528	9
GTACATG	17295	0.0	18.21552	1
TCTAACG	645	0.0	18.214699	2
GAGTACG	700	0.0	18.136486	1
CCGTATC	235	4.9039954E-9	18.001863	94
ACATGGG	16400	0.0	17.880613	3
GTACCCG	290	5.8635123E-8	17.835367	1
CTAGTAC	1795	0.0	17.80271	3
TACATGG	17185	0.0	17.774832	2
CTCGTCG	955	0.0	17.714396	9
CTAACGC	640	0.0	17.62272	3
CAATGCG	860	0.0	17.211172	18-19
TGGGGGG	4020	0.0	17.066591	6
>>END_MODULE