FastQCFastQC Report
Mon 16 Jan 2017
ERR1146546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006444
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT306331.0189113783592842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77360.25731395628855885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69230.23027204231976384No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG58820.19564641816045802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.1286902400310799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC7650.021.5103364
GAGTACT87700.021.2744412-13
TCTAACG6750.020.911762
CTAACGC6800.020.7424553
CTAGTAC19700.020.524853
CATGGGG60500.020.5158334
TAGTACT22200.020.1192234
CTGTGCG18700.020.1055349
AGTACTT94400.019.7147112-13
GTACCCG4100.019.510441
CCGACGT1456.604956E-419.4475594
GCTATTG21700.019.2751378
CTATTGA21300.018.9751859
GGGCTAT22600.018.9240726
CGCCTAC7300.018.6699147
GGCTATT22750.018.5920947
GTACTTT101250.018.28809214-15
ATTTAGG24800.017.8352091
GTCCTAC10850.017.7811
CCTAGTA23200.017.4414882
Produced by FastQC (version 0.11.5)