##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006444
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43814553006808	34.0	31.0	34.0	31.0	34.0
2	32.4572155676274	34.0	31.0	34.0	31.0	34.0
3	32.62489938279243	34.0	31.0	34.0	31.0	34.0
4	36.11558838282037	37.0	35.0	37.0	35.0	37.0
5	36.15822213884576	37.0	35.0	37.0	35.0	37.0
6	36.26758090288727	37.0	37.0	37.0	35.0	37.0
7	36.25162018650605	37.0	37.0	37.0	35.0	37.0
8	36.26194001950477	37.0	37.0	37.0	35.0	37.0
9	38.068143627488155	39.0	38.0	39.0	35.0	39.0
10-11	38.0388666810358	39.0	38.0	39.0	35.0	39.0
12-13	38.03407480731389	39.0	38.0	39.0	35.0	39.0
14-15	39.46524598495765	41.0	39.0	41.0	37.0	41.0
16-17	39.466852866708976	41.0	39.0	41.0	36.5	41.0
18-19	39.51636817449452	41.0	39.0	41.0	37.0	41.0
20-21	39.54477914772403	41.0	39.0	41.0	37.0	41.0
22-23	39.50798501485476	41.0	39.0	41.0	37.0	41.0
24-25	39.493916567213624	41.0	39.0	41.0	36.0	41.0
26-27	39.411588907027706	41.0	39.0	41.0	36.0	41.0
28-29	39.33363335555228	41.0	39.0	41.0	36.0	41.0
30-31	39.27043876420116	41.0	39.0	41.0	36.0	41.0
32-33	39.16654559339871	41.0	39.0	41.0	35.0	41.0
34-35	39.093535086633906	41.0	39.0	41.0	35.0	41.0
36-37	39.0212435355523	40.5	39.0	41.0	35.0	41.0
38-39	38.92934160756029	40.0	39.0	41.0	35.0	41.0
40-41	38.87519059061137	40.0	39.0	41.0	35.0	41.0
42-43	38.79741116082654	40.0	38.0	41.0	35.0	41.0
44-45	38.67244758259259	40.0	38.0	41.0	35.0	41.0
46-47	38.472214350242346	40.0	38.0	41.0	35.0	41.0
48-49	38.27344414198302	40.0	38.0	41.0	34.0	41.0
50-51	38.114954910186256	40.0	38.0	41.0	34.0	41.0
52-53	38.02424342512283	40.0	37.5	41.0	34.0	41.0
54-55	37.89225377223058	40.0	37.0	41.0	34.0	41.0
56-57	37.66693392592711	40.0	36.5	41.0	33.0	41.0
58-59	37.458303563944646	39.5	36.0	41.0	33.0	41.0
60-61	37.22647885674904	39.0	35.5	41.0	33.0	41.0
62-63	36.979630254213944	39.0	35.0	41.0	33.0	41.0
64-65	36.69531330036415	38.5	35.0	41.0	32.0	41.0
66-67	36.40670306847558	37.5	35.0	40.0	32.0	41.0
68-69	36.05923110491997	37.0	35.0	40.0	31.5	41.0
70-71	35.72416981656735	36.5	35.0	39.0	31.0	41.0
72-73	35.38526228993456	36.0	35.0	39.0	31.0	41.0
74-75	35.0728774592176	35.5	35.0	38.5	31.0	40.5
76-77	34.761042281180025	35.0	35.0	37.0	31.0	39.5
78-79	34.462429035764515	35.0	34.5	37.0	31.0	39.0
80-81	34.1940413658129	35.0	34.0	36.5	30.5	39.0
82-83	33.93502007687487	35.0	34.0	36.0	30.0	37.0
84-85	33.7379121314084	35.0	34.0	36.0	30.0	37.0
86-87	33.595290815328674	35.0	34.0	35.0	30.0	37.0
88-89	33.44767506063642	35.0	34.0	35.0	30.0	36.0
90-91	33.3465733936837	35.0	34.0	35.0	30.0	36.0
92-93	33.24931447251304	35.0	34.0	35.0	30.0	36.0
94-95	33.16002110799336	35.0	34.0	35.0	29.5	36.0
96-97	33.117970931771886	35.0	34.0	35.0	29.0	35.5
98-99	33.09729002103482	35.0	34.0	35.0	29.0	35.0
100	32.50711405234889	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.216665427023905
1101	2	0.2219550623464741
1101	3	0.2653073487939821
1101	4	0.1799704007101468
1101	5	0.12200822656142662
1101	6	0.1368031940035408
1101	7	0.21724355611846846
1101	8	0.17917851036223453
1101	9	0.21776260775401823
1101	10-11	0.20461019777182798
1101	12-13	0.2325279418840367
1101	14-15	0.3259564486715263
1101	16-17	0.3431370521546384
1101	18-19	0.33616990811682257
1101	20-21	0.31309289461706413
1101	22-23	0.3285534415111755
1101	24-25	0.3525654879374258
1101	26-27	0.36029844019968493
1101	28-29	0.3630994125243987
1101	30-31	0.3798870464596149
1101	32-33	0.3697040489438592
1101	34-35	0.34348368875588875
1101	36-37	0.357730521712881
1101	38-39	0.39734962645520255
1101	40-41	0.3715280733538222
1101	42-43	0.30842357481605376
1101	44-45	0.3193085755987184
1101	46-47	0.29572129253621426
1101	48-49	0.3529403581094357
1101	50-51	0.2865790882340207
1101	52-53	0.2769348240907519
1101	54-55	0.2967836085965203
1101	56-57	0.2903257827968204
1101	58-59	0.2894712871238525
1101	60-61	0.3049750142930421
1101	62-63	0.24740374913531582
1101	64-65	0.2673736190871878
1101	66-67	0.18566870721176798
1101	68-69	0.21105789989372425
1101	70-71	0.22471733762963453
1101	72-73	0.19104090233941662
1101	74-75	0.2404401591062637
1101	76-77	0.2067736213110436
1101	78-79	0.22019035590686542
1101	80-81	0.1633557874944742
1101	82-83	0.16400944163861197
1101	84-85	0.2438360255765275
1101	86-87	0.2366439062049821
1101	88-89	0.2600067823590564
1101	90-91	0.26718369917829676
1101	92-93	0.250981777197957
1101	94-95	0.24063990686159542
1101	96-97	0.21965840444288176
1101	98-99	0.20902566433857572
1101	100	0.15805649156953905
1102	1	0.22157771338650178
1102	2	0.2226572688924051
1102	3	0.24248906440033124
1102	4	0.15806116658651348
1102	5	0.15517273051126068
1102	6	0.14356221770385247
1102	7	0.16273079005589608
1102	8	0.19508860426202546
1102	9	0.20703926980586118
1102	10-11	0.18418002735936057
1102	12-13	0.22345172397145774
1102	14-15	0.28214789574739285
1102	16-17	0.2928187030593037
1102	18-19	0.24789378619801283
1102	20-21	0.2799220381381744
1102	22-23	0.2576397898589988
1102	24-25	0.28948090821436523
1102	26-27	0.26526000067659794
1102	28-29	0.3231335346805082
1102	30-31	0.2901769671471115
1102	32-33	0.16731596857394493
1102	34-35	0.2330713222304226
1102	36-37	0.22714339677678197
1102	38-39	0.2936211624530429
1102	40-41	0.25540885016393133
1102	42-43	0.19409872261319094
1102	44-45	0.2268764879997036
1102	46-47	0.1821331992595816
1102	48-49	0.18495693652972278
1102	50-51	0.16157822274394817
1102	52-53	0.1821918611972606
1102	54-55	0.23441441103521043
1102	56-57	0.18256581562830831
1102	58-59	0.2388123886927218
1102	60-61	0.28599866553179965
1102	62-63	0.20762515325934316
1102	64-65	0.19801821928622587
1102	66-67	0.11334405465981234
1102	68-69	0.1410185108524047
1102	70-71	0.15479878108557443
1102	72-73	0.11221905784059061
1102	74-75	0.1299584189300731
1102	76-77	0.1264916026319156
1102	78-79	0.15912914071847695
1102	80-81	0.17394831039970882
1102	82-83	0.17272813652664354
1102	84-85	0.17741448586465225
1102	86-87	0.08074847353893233
1102	88-89	0.10568512994860413
1102	90-91	0.1271901592634137
1102	92-93	0.10183108344465097
1102	94-95	0.1225103939190717
1102	96-97	0.14066107553009743
1102	98-99	0.16504532423963525
1102	100	0.18465389214154015
1103	1	0.2500927599115954
1103	2	0.22638031710994966
1103	3	0.2748213769444803
1103	4	0.17152564185116148
1103	5	0.15182135128981145
1103	6	0.16460400069770742
1103	7	0.14691896208026378
1103	8	0.15587954825502948
1103	9	0.2057271568647252
1103	10-11	0.21786491447718248
1103	12-13	0.23833437945683755
1103	14-15	0.3126984514012179
1103	16-17	0.30034312165454224
1103	18-19	0.2809018080056731
1103	20-21	0.2578239175232042
1103	22-23	0.26517721397898697
1103	24-25	0.2955252980934091
1103	26-27	0.32952781275427867
1103	28-29	0.37436142962361174
1103	30-31	0.33305444816772223
1103	32-33	0.3264897189164202
1103	34-35	0.2678544536602061
1103	36-37	0.30618086078037976
1103	38-39	0.31600973978735425
1103	40-41	0.2994007601495099
1103	42-43	0.30524191401867284
1103	44-45	0.3298348691644648
1103	46-47	0.40525627082926974
1103	48-49	0.4468827137109841
1103	50-51	0.4306162878918016
1103	52-53	0.3580207755422933
1103	54-55	0.3181095869681698
1103	56-57	0.3952162606484606
1103	58-59	0.33035914442122305
1103	60-61	0.3383210476352758
1103	62-63	0.32414136045527187
1103	64-65	0.3322499742690823
1103	66-67	0.275213416764835
1103	68-69	0.36947095745172476
1103	70-71	0.36091496146121926
1103	72-73	0.27989899939086627
1103	74-75	0.2502155930665353
1103	76-77	0.18630776809455796
1103	78-79	0.22889395458918216
1103	80-81	0.2581989667451907
1103	82-83	0.23000995873360353
1103	84-85	0.22761610039939484
1103	86-87	0.2281871943964333
1103	88-89	0.24714073139922021
1103	90-91	0.2600707847308925
1103	92-93	0.2475452717207105
1103	94-95	0.1676019932749213
1103	96-97	0.22319384656234575
1103	98-99	0.24567379743727003
1103	100	0.2172451591339808
1104	1	0.20108818430132658
1104	2	0.18400411698861774
1104	3	0.2169227337204589
1104	4	0.15344718416957903
1104	5	0.10791774833109002
1104	6	0.11080428966484845
1104	7	0.06964379167632018
1104	8	0.10327251324977738
1104	9	0.18555010296711316
1104	10-11	0.1865817783954924
1104	12-13	0.1844944548408094
1104	14-15	0.2689821848909091
1104	16-17	0.24558051375998247
1104	18-19	0.2671116697672389
1104	20-21	0.2628855682453164
1104	22-23	0.25397608150742457
1104	24-25	0.2641237503965286
1104	26-27	0.2643956286556062
1104	28-29	0.32739606609103333
1104	30-31	0.30517716183381083
1104	32-33	0.2780110406753735
1104	34-35	0.29416216193536115
1104	36-37	0.32432075340387456
1104	38-39	0.3326160771627329
1104	40-41	0.33120453284344364
1104	42-43	0.3478482499674129
1104	44-45	0.28424595149299137
1104	46-47	0.3608471893717109
1104	48-49	0.2765844305724059
1104	50-51	0.2755343431308219
1104	52-53	0.29497764696475315
1104	54-55	0.26985836959333653
1104	56-57	0.31503064330843245
1104	58-59	0.2915748821808606
1104	60-61	0.25363721556434626
1104	62-63	0.28714682557931326
1104	64-65	0.33986371629368506
1104	66-67	0.34592093329877116
1104	68-69	0.362415390661333
1104	70-71	0.22599598246893748
1104	72-73	0.1898642641282109
1104	74-75	0.23367844802051962
1104	76-77	0.1926233514554383
1104	78-79	0.21990349920735497
1104	80-81	0.1976363296757242
1104	82-83	0.19731595895687803
1104	84-85	0.2702720122954432
1104	86-87	0.17563793855280352
1104	88-89	0.26374599879012095
1104	90-91	0.19063200228762156
1104	92-93	0.15624536898229735
1104	94-95	0.12909200372153862
1104	96-97	0.13891553860725736
1104	98-99	0.12190441333103053
1104	100	0.16057806804013808
1105	1	0.2352692017072613
1105	2	0.16518773439270973
1105	3	0.19346089772867003
1105	4	0.13002869549946894
1105	5	0.10356439868503031
1105	6	0.09161393127558881
1105	7	0.12892442240508473
1105	8	0.13790304182073498
1105	9	0.12286757667623505
1105	10-11	0.13430560941270642
1105	12-13	0.13892259684170227
1105	14-15	0.17947627426209323
1105	16-17	0.19109321079613295
1105	18-19	0.1725886010572495
1105	20-21	0.22216619897407952
1105	22-23	0.24149488474002112
1105	24-25	0.1894732136439714
1105	26-27	0.19236379164601658
1105	28-29	0.23884127308571834
1105	30-31	0.24023387364506021
1105	32-33	0.21336064131084242
1105	34-35	0.2579499888522534
1105	36-37	0.209253157096974
1105	38-39	0.22387275975800236
1105	40-41	0.222916194086082
1105	42-43	0.17259533685074757
1105	44-45	0.2397630757879341
1105	46-47	0.28605646076379543
1105	48-49	0.23311752524312368
1105	50-51	0.2503921955990762
1105	52-53	0.18892424676551656
1105	54-55	0.20776187329622786
1105	56-57	0.27734435286623693
1105	58-59	0.23532929296711558
1105	60-61	0.1854264197260207
1105	62-63	0.20019462174932556
1105	64-65	0.19028553956727023
1105	66-67	0.18039214784530344
1105	68-69	0.22378745956507373
1105	70-71	0.19322543531975356
1105	72-73	0.2307964165975136
1105	74-75	0.24191093434269106
1105	76-77	0.1490348156724508
1105	78-79	0.21153898019579742
1105	80-81	0.2251821226443198
1105	82-83	0.2467480557700057
1105	84-85	0.20554607755924081
1105	86-87	0.22312120034623462
1105	88-89	0.19195983398785899
1105	90-91	0.1465946843372592
1105	92-93	0.16388997477822898
1105	94-95	0.19087898444038842
1105	96-97	0.1678216972635056
1105	98-99	0.16675512442618157
1105	100	0.2891319806406045
1106	1	0.09760739909900451
1106	2	0.050736510742666496
1106	3	0.10718850741808694
1106	4	0.06571508403064996
1106	5	0.0567897755460649
1106	6	0.052789405649356524
1106	7	0.06197368893390376
1106	8	0.007364939433841755
1106	9	0.07037345815192708
1106	10-11	0.08664858386896412
1106	12-13	0.07293470606990837
1106	14-15	0.14674962722761364
1106	16-17	0.1377203645320435
1106	18-19	0.13267755477283316
1106	20-21	0.06792945774211745
1106	22-23	0.0965369140563368
1106	24-25	0.09114231622908875
1106	26-27	0.09147157225295643
1106	28-29	0.18164908193661944
1106	30-31	0.13962420112208207
1106	32-33	0.08403558071239559
1106	34-35	0.05862186245665413
1106	36-37	0.12993948259558863
1106	38-39	0.1496579377687013
1106	40-41	0.12272720365066903
1106	42-43	0.16107504746953794
1106	44-45	0.12427184147332326
1106	46-47	0.12072448428487093
1106	48-49	0.1181309538638331
1106	50-51	0.17085943596053
1106	52-53	0.20057542932367767
1106	54-55	0.16000576590909787
1106	56-57	0.23581613210953378
1106	58-59	0.13900645245643517
1106	60-61	0.2163918025040772
1106	62-63	0.17624709811143902
1106	64-65	0.1744353146214479
1106	66-67	0.131152092709236
1106	68-69	0.1561420266506417
1106	70-71	0.11646637720345154
1106	72-73	0.05478379828066693
1106	74-75	0.08114675422617523
1106	76-77	0.04050286043684537
1106	78-79	0.030597777786397273
1106	80-81	0.07630771008779647
1106	82-83	0.21941200819141216
1106	84-85	0.16900131946887598
1106	86-87	0.09796532883505904
1106	88-89	0.07580187596433774
1106	90-91	0.13082484604129263
1106	92-93	0.10715655348539599
1106	94-95	0.08888173695223855
1106	96-97	0.0632592385531936
1106	98-99	0.04235835287420997
1106	100	0.0013443408725777317
1107	1	0.06732092543539636
1107	2	0.0941521288827758
1107	3	0.06140319277843531
1107	4	0.039925894224133174
1107	5	0.09766116754527587
1107	6	0.007789271454328173
1107	7	-0.00275549915471629
1107	8	0.005114217355497885
1107	9	0.037684056327407234
1107	10-11	0.06173455366955238
1107	12-13	0.017201227241095296
1107	14-15	0.09461331085123703
1107	16-17	0.10182707540477764
1107	18-19	0.08358386335473966
1107	20-21	0.11202439699641076
1107	22-23	0.11271167687741013
1107	24-25	0.16127966547593786
1107	26-27	0.1276895423220452
1107	28-29	0.1443116361302188
1107	30-31	0.14238828894925604
1107	32-33	0.0857011718739642
1107	34-35	0.09148691010045695
1107	36-37	0.1727221771089944
1107	38-39	0.24212906896784858
1107	40-41	0.11780919681368118
1107	42-43	0.11792926534614878
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2216	56-57	-0.748607565903356
2216	58-59	-0.6926350994820254
2216	60-61	-0.8131116451223903
2216	62-63	-0.8564945079280335
2216	64-65	-0.7924694469830555
2216	66-67	-0.7635192509423874
2216	68-69	-0.8297111868113092
2216	70-71	-0.7802569589169082
2216	72-73	-0.8023633585992656
2216	74-75	-0.8273087898253877
2216	76-77	-0.798913432840294
2216	78-79	-0.7922721573890641
2216	80-81	-0.8415329109095424
2216	82-83	-0.7793205061861386
2216	84-85	-0.7748392561290061
2216	86-87	-0.7736307521827968
2216	88-89	-0.6708626930791937
2216	90-91	-0.6581876863110736
2216	92-93	-0.6847941645616942
2216	94-95	-0.7366637396432907
2216	96-97	-0.7133039635883094
2216	98-99	-0.7373353941490848
2216	100	-0.9530926364819194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	21.0
8	65.0
9	116.0
10	205.0
11	197.0
12	148.0
13	159.0
14	215.0
15	428.0
16	820.0
17	1480.0
18	2778.0
19	4397.0
20	6292.0
21	9235.0
22	14046.0
23	17069.0
24	16985.0
25	15717.0
26	14546.0
27	15910.0
28	19601.0
29	24314.0
30	30467.0
31	39195.0
32	50873.0
33	67841.0
34	101077.0
35	161746.0
36	304169.0
37	735693.0
38	999515.0
39	350208.0
40	914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60337417160056	19.7844889096013	13.351023152107494	23.26111376669065
2	14.952882132760934	21.875853041788893	38.891947818143805	24.279317007306364
3	19.323744127598257	26.381037214828456	30.68886216104384	23.606356496529447
4	12.061292344044992	17.65331401482948	39.85362774094578	30.431765900179748
5	12.146985805396273	39.39810274991124	35.4697389309609	12.985172513731587
6	29.06129348686226	38.55806015223167	18.16764509923864	14.21300126166743
7	25.473216863510512	33.40132062995353	23.386366085648028	17.739096420887932
8	25.37393013141106	33.53855917489233	20.966962963554288	20.12054773014232
9	25.72637308394901	15.978544752538216	21.53893436897544	36.756147794537334
10-11	24.507956908560413	25.661745237895666	28.765445157135805	21.064852696408114
12-13	26.574867185286006	23.708391042706932	28.179570283032046	21.537171488975016
14-15	23.18004645688502	25.049963520019585	27.306500188678086	24.46348983441731
16-17	21.449609571972736	27.830935816532755	28.106227822636974	22.613226788857535
18-19	21.187921677569914	28.338079804579763	29.264955542162102	21.209042975688224
20-21	22.798661807770245	26.580205718117483	28.31401482947961	22.307117644632662
22-23	22.209748946216067	26.41009969473736	28.328123661721428	23.052027697325148
24-25	22.87666093231738	26.67621615436709	28.23308865889403	22.214034254421502
26-27	22.672582625853003	25.84538411492115	29.392614663702364	22.089418595523483
28-29	21.77144582010133	27.665438470883778	28.68751023842964	21.875605470585253
30-31	22.482321283644442	26.288567209057888	28.554902143398834	22.674209363898832
32-33	22.425953124171034	26.93689556858817	28.59641107714087	22.040740230099924
34-35	22.941350804500132	26.73580470469508	28.74071023792235	21.582134252882433
36-37	22.848648649547627	25.861951687697506	29.25700463857577	22.032395024179102
38-39	22.631712170908752	26.612023657349614	28.990498010426347	21.76576616131529
40-41	22.40613558253691	26.756940823693476	28.757640997240053	22.079282596529566
42-43	22.48530895353049	26.582087750126977	27.743185173876068	23.189418122466464
44-45	22.79932291720371	26.269505296486496	28.12526297827953	22.80590880803026
46-47	23.362124378312167	26.864597824830593	27.923377624476853	21.849900172380387
48-49	23.271864875832385	26.713050738087162	27.886339899282202	22.12874448679825
50-51	21.720131964241197	27.073175558774963	28.496047043319457	22.710645433664386
52-53	22.48789790718239	27.670040079477076	26.84972383096536	22.992338182375175
54-55	22.2293828792405	26.7779504835855	27.359610191202293	23.633056445971707
56-57	23.33971026245418	26.128866966104596	27.356277527636365	23.175145243804863
58-59	23.596557695390903	26.416414811542737	27.46281380993215	22.52421368313421
60-61	22.896303769275214	27.08879462291448	28.179954876068848	21.834946731741457
62-63	22.27545288612258	26.974309081006016	27.7924103894592	22.957827643412205
64-65	21.649634907812622	28.059999424540933	27.184985640965763	23.105380026680677
66-67	23.020528712772155	26.438499265625886	26.877377402100223	23.663594619501737
68-69	22.617729365740143	26.442684302385338	27.309440801936454	23.63014552993807
70-71	23.05992467380324	26.45574045425229	27.094912138840222	23.389422733104244
72-73	23.062386851535077	25.724312335916533	28.41133526207201	22.80196555047638
74-75	22.40403722555335	27.83599028979597	27.090810631728736	22.669161852921942
76-77	22.62297683037632	26.367743910886652	27.00977288410099	23.99950637463604
78-79	22.620802792425607	26.77313554317205	27.302453068326983	23.303608596075364
80-81	22.965012142255816	26.520126940707005	27.45481953258385	23.06004138445332
82-83	22.82905883972994	25.93126816518418	28.30688675435848	22.9327862407274
84-85	22.645134670052506	26.955813693968	27.323025351740704	23.076026284238786
86-87	22.175803403589654	27.24428381907061	27.325426205415138	23.254486571924602
88-89	22.250600211945166	26.85106095652064	26.778350326865212	24.119988504668978
90-91	22.726350465865984	25.928572093809166	27.080664066917592	24.264413373407255
92-93	23.2954447180789	26.814485817796708	27.06268601710193	22.827383447022463
94-95	22.415704802036025	26.47089492950724	27.75325729553873	23.360142972918002
96-97	21.759876531403915	26.45680553280341	28.37056877212444	23.412749163668234
98-99	22.27584067801402	27.08521991632927	27.01541946379881	23.623519941857904
100	22.35481638109417	25.68928395495369	26.667824190722712	25.288075473229426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	269.0
1	174.0
2	279.5
3	444.0
4	265.0
5	176.0
6	361.0
7	901.0
8	1760.5
9	2155.5
10	1597.5
11	992.0
12	973.5
13	1165.0
14	1362.5
15	1640.5
16	1932.5
17	2247.5
18	2657.0
19	3114.5
20	3582.0
21	4135.0
22	4924.0
23	6152.0
24	7678.5
25	9466.0
26	12225.0
27	15442.0
28	18880.5
29	24297.0
30	30463.0
31	35631.5
32	42420.5
33	49225.0
34	54531.0
35	59953.5
36	66665.5
37	74710.0
38	79099.0
39	81024.0
40	82131.0
41	82638.0
42	85253.0
43	91993.0
44	103988.0
45	115708.0
46	119843.5
47	141750.5
48	189287.5
49	187071.0
50	153222.5
51	132528.0
52	110804.5
53	101530.0
54	95870.0
55	86091.0
56	77723.0
57	67821.0
58	57863.5
59	52741.5
60	44385.0
61	33649.0
62	24868.0
63	18424.0
64	13125.0
65	8735.0
66	6065.0
67	4277.0
68	2908.0
69	1983.0
70	1647.5
71	1415.5
72	1122.0
73	957.5
74	741.0
75	477.5
76	329.0
77	242.0
78	165.0
79	78.5
80	40.0
81	29.0
82	15.5
83	11.5
84	13.0
85	14.5
86	12.0
87	7.0
88	5.0
89	1.0
90	1.0
91	1.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0064860679260947485
2	0.08275557435960888
3	2.660950944038871E-4
4	0.0
5	0.038151384160157314
6	0.0034924981140510188
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6630943400242944E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.488211322080172E-4
24-25	0.0
26-27	0.0
28-29	4.989283020072884E-5
30-31	1.3304754720194355E-4
32-33	0.0018127728306264808
34-35	0.0014302611324208932
36-37	8.481781134123902E-4
38-39	6.984996228102037E-4
40-41	0.0015466777362225937
42-43	6.984996228102037E-4
44-45	5.654520756082601E-4
46-47	3.8251169820558773E-4
48-49	7.982852832116614E-4
50-51	7.816543398114184E-4
52-53	0.0014136301890206502
54-55	0.0015633086796228368
56-57	0.0011142732078162773
58-59	0.0028605222648417864
60-61	0.0018959275476276957
62-63	0.0010477494342153054
64-65	0.0049393901898721546
66-67	0.004739818869069239
68-69	0.004024688302858793
70-71	0.0017628800004257522
72-73	0.0053717947182784715
74-75	0.004057950189659279
76-77	0.00384174792545612
78-79	8.481781134123902E-4
80-81	3.326188680048589E-5
82-83	1.9957132080291535E-4
84-85	1.1641660380170062E-4
86-87	1.6630943400242944E-5
88-89	1.9957132080291535E-4
90-91	0.0
92-93	0.0
94-95	1.6630943400242944E-5
96-97	3.8251169820558773E-4
98-99	5.488211322080172E-4
100	8.980709436131189E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3006444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.840231500626416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.5505485561259	38.690084153141285
2	15.346821424853033	16.832464794754273
3	5.986879048190739	9.8496549890709
4	2.995346042297544	6.570618815363298
5	1.660646056645399	4.5535107093518015
6	0.9404567651287687	3.0944920029595147
7	0.6518814291196328	2.50245299387159
8	0.43751013887894374	1.9194525839993988
9	0.3074069428298293	1.51724411187189
>10	1.0889315623251488	9.262840594997627
>50	0.022437680254267484	0.843931807494371
>100	0.009422033890220323	0.9517202821109625
>500	0.0010464174481609253	0.38683229308364225
>1k	2.853865767711606E-4	0.35500927234446117
>5k	2.853865767711606E-4	1.0716010967270504
>10k+	9.512885892372022E-5	1.5980894988578809
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	30633	1.0189113783592842	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7736	0.25731395628855885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6923	0.23027204231976384	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	5882	0.19564641816045802	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3869	0.1286902400310799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.326188680048589E-5	0.0
4	0.0	0.0	0.0	3.326188680048589E-5	0.0
5	0.0	0.0	0.0	3.326188680048589E-5	0.0
6	0.0	0.0	0.0	3.326188680048589E-5	0.0
7	0.0	0.0	0.0	3.326188680048589E-5	0.0
8	0.0	0.0	0.0	3.326188680048589E-5	0.0
9	0.0	0.0	0.0	3.326188680048589E-5	0.0
10-11	0.0	0.0	0.0	3.326188680048589E-5	0.0
12-13	0.0	0.0	0.0	3.326188680048589E-5	0.0
14-15	0.0	0.0	0.0	3.326188680048589E-5	0.0
16-17	0.0	0.0	0.0	6.652377360097177E-5	0.0
18-19	0.0	0.0	0.0	1.164166038017006E-4	0.0
20-21	0.0	0.0	0.0	1.829403774026724E-4	0.0
22-23	0.0	0.0	0.0	5.987139624087461E-4	0.0
24-25	0.0	0.0	0.0	0.0012972135852189497	0.0
26-27	0.0	0.0	0.0	0.0022784392458332836	0.0
28-29	0.0	0.0	0.0	0.006219972831690861	0.0
30-31	0.0	0.0	0.0	0.016747360004044646	0.0
32-33	0.0	0.0	0.0	0.04097864453819862	0.0
34-35	0.0	0.0	0.0	0.07783281511313699	0.0
36-37	0.0	0.0	0.0	0.129039489842485	0.0
38-39	0.0	0.0	0.0	0.20687230495562198	0.0
40-41	0.0	0.0	0.0	0.29080867629664814	0.0
42-43	0.0	0.0	0.0	0.37010501442900645	0.0
44-45	0.0	0.0	0.0	0.46227370275315294	0.0
46-47	0.0	0.0	0.0	0.5757133676862101	0.0
48-49	0.0	0.0	0.0	0.6862758794110251	0.0
50-51	0.0	0.0	0.0	0.8061184575531757	0.0
52-53	0.0	0.0	0.0	0.9189261466370237	0.0
54-55	0.0	0.0	0.0	1.0445562930824588	0.0
56-57	0.0	0.0	0.0	1.1882975368907587	0.0
58-59	0.0	0.0	0.0	1.3406369784369838	0.0
60-61	0.0	0.0	0.0	1.4963691324368589	0.0
62-63	0.0	0.0	0.0	1.6536645951163567	0.0
64-65	0.0	0.0	0.0	1.818726708363768	0.0
66-67	0.0	0.0	0.0	1.998640254067596	0.0
68-69	0.0	0.0	0.0	2.19879365788952	0.0
70-71	0.0	0.0	0.0	2.415993778696693	0.0
72-73	0.0	0.0	0.0	2.617461027047236	0.0
74-75	0.0	0.0	0.0	2.8252480338898716	0.0
76-77	0.0	0.0	0.0	3.040518965262616	0.0
78-79	0.0	0.0	0.0	3.300177884570609	0.0
80-81	0.0	0.0	0.0	3.5660734076536933	0.0
82-83	0.0	0.0	0.0	3.823636828093256	0.0
84-85	0.0	0.0	0.0	4.084992103628073	0.0
86-87	0.0	0.0	0.0	4.347644592748111	0.0
88	0.0	0.0	0.0	4.562399964875447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	765	0.0	21.510336	4
GAGTACT	8770	0.0	21.27444	12-13
TCTAACG	675	0.0	20.91176	2
CTAACGC	680	0.0	20.742455	3
CTAGTAC	1970	0.0	20.52485	3
CATGGGG	6050	0.0	20.515833	4
TAGTACT	2220	0.0	20.119223	4
CTGTGCG	1870	0.0	20.105534	9
AGTACTT	9440	0.0	19.71471	12-13
GTACCCG	410	0.0	19.51044	1
CCGACGT	145	6.604956E-4	19.44755	94
GCTATTG	2170	0.0	19.275137	8
CTATTGA	2130	0.0	18.975185	9
GGGCTAT	2260	0.0	18.924072	6
CGCCTAC	730	0.0	18.669914	7
GGCTATT	2275	0.0	18.592094	7
GTACTTT	10125	0.0	18.288092	14-15
ATTTAGG	2480	0.0	17.835209	1
GTCCTAC	1085	0.0	17.781	1
CCTAGTA	2320	0.0	17.441488	2
>>END_MODULE