FastQCFastQC Report
Mon 16 Jan 2017
ERR1146545_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146545_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933960
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT242040.6152579080621053No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC56970.14481591068541624No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC46310.1177185329794914No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA40710.10348351279626634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16500.027.0694391
GCATCGT14250.023.7421348
TAGGCAT19600.021.8171735
TATACCG4950.020.8848155
CATGGGG70350.019.9051444
CGACGAG8400.019.01901224-25
CTAGTAC21550.018.7527243
TAGTACT24400.018.6807824
GTATAAG17900.018.3858871
GTATATA24150.018.2999671
CAATGCG9400.017.99591818-19
TACCTGG39500.017.9635852
CATCGTC20550.017.8354849
GTACCTG42300.017.783581
CTGTGCG20250.017.1715229
CTAGGCA28550.017.1175024
TGCGACG9400.016.99495722-23
GTGATCG9250.016.7638728
ACATGGG230650.016.6041763
TACTAGG26100.016.563822
Produced by FastQC (version 0.11.5)